• 대한수의학회지
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• 제52권2호
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• pp.75-82
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• 2012

We have developed and optimized a multiplex polymerase chain reaction (mPCR) for simultaneous detection of Brucella, Salmonella and Leptospira with high sensitivity and specificity. Three pairs of oligonucleotide primers were designed to specifically amplify the targeted genes of Salmonella, Leptospira and Brucella species with sizes of 521, 408 and 223 bp, respectively. The mPCR did not produce any nonspecific amplification products when tested against 15 related species of bacteria. The sensitivity of the mPCR was 100 fg for Brucella and 1 pg for both Salmonella and Leptospira species. In the field application, kidney, liver and spleen were collected from wild rats and stray cats and examined by mPCR. The high specificity and sensitivity of this mPCR assay provide a valuable tool for diagnosis and for the simultaneous and rapid detection of three zoonotic bacteria that cause disease in both humans and animals. Therefore, this assay could be a useful alternative to the conventional method of culture and single PCR for the detection of each pathogen.

• Journal of Magnetics
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• 제18권2호
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• pp.135-141
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• 2013

This paper proposes an efficient fault detection method for onboard degaussing coils which are installed to minimize underwater magnetic fields due to the ferromagnetic hull. To achieve this, the method basically uses field signals measured at specific magnetic treatment facilities instead of time-consuming numerical field solutions in a three-dimensional analysis space. In addition, an analytical design sensitivity formula and the linear property of degaussing coil fields is being exploited for detecting fault coil positions and assessing individual degaussing coil currents. Such peculiar features make it possible to yield fast and accurate results on the fault detection of degaussing coils. For foreseeable fault conditions, the proposed method is tested with a model ship equipped with 20 degaussing coils.

• Journal of Gastric Cancer
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• 제14권1호
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• pp.1-6
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• 2014

Aerobic glycolysis has been the most important hypothesis in cancer metabolism. It seems to be related to increased bioenergetic and biosynthetic needs in rapidly proliferating cancer cells. To this end, F-18 fluorodeoxyglucose (FDG), a glucose analog, became widely popular for the detection of malignancies combined with positron emission tomography/computed tomography (PET/CT). Although the potential roles of FDG PET/CT in primary tumor detection are not fully established, it seems to have a limited sensitivity in detecting early gastric cancer and mainly signet ring or non-solid types of advanced gastric cancer. In evaluating lymph node metastases, the location of lymph nodes and the degree of FDG uptake in primary tumors appear to be important factors affecting the diagnostic accuracy of PET/CT. In spite of the limited sensitivity, the high specificity of PET/CT for lymph node metastases may play an important role in changing the extent of lymphadenectomy or reducing futile laparotomies. For peritoneal metastases, PET/CT seems to have a poorer sensitivity but a better specificity than CT. The roles of PET/CT in the evaluation of other distant metastases are yet to be known. Studies including primary tumors with low FDG uptake or peritoneal recurrence seem suffer from poorer diagnostic performance for the detection of recurrent gastric cancer. There are only a few reports using FDG PET/CT to predict response to neoadjuvant or adjuvant chemotherapy. A complete metabolic response seems to be predictive of more favorable prognosis.

• 한국해양공학회지
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• 제26권5호
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• pp.11-17
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• 2012

In this study, a damage detection method that uses sensitivity-based finite (FE) element model updating with the natural frequency and zero frequency was proposed. The stiffness matrix for a structure was modified using the sensitivity-based FE model updating method. A sensitivity analysis was used to update the FE model, and the natural frequencies and zero frequencies were considered as target parameters to supplement the information on the vibration characteristics. The locations and values of the damages were estimated from the modified stiffness matrix. Several numerical examples were considered to verify the performance of the proposed method.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3867-3870
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• 2015

Background: To explore whether combined detection of serum tumor markers (CEA, CA72-4, CA19-9 and TSGF) improve the sensitivity and accuracy in the diagnosis of gastric cancer (GC). Materials and Methods: An automatic chemiluminescence immune analyzer with matched kits were used to determine the levels of serum CEA, CA72-4, CA19-9 and TSGF in 45 patients with gastric cancer (GC group), 40 patients with gastric benign diseases (GBD group) hospitalized in the same period and 30 healthy people undergoing a physical examination. The values of those 4 tumor markers in the diagnosis of gastric cancer was analyzed. Results: The levels of serum CEA, CA72-4, CA19-9 and TSGF of the GC group were higher than those of the GBD group and healthy examined people and the differences were significant (P<0.001). The area under receiver operating characteristic (ROC) curves for single detection of CEA, CA72-4, CA19-9 and TSGF in the diagnosis of GC was 0.833, 0.805, 0.810 and 0.839, respectively. The optimal cutoff values for these 4 indices were 2.36 ng/mL, 3.06 U/mL, 5.72 U/mL and 60.7 U/mL, respectively. With combined detection of tumor markers, the diagnostic power of those 4 indices was best, with an area under the ROC curve of 0.913 (95%CI 0.866~0.985), a sensitivity of 88.9% and a diagnostic accuracy of 90.4%. Conclusions: Combined detection of serum CEA, CA72-4, CA19-9 and TSGF increases the sensitivity and accuracy in diagnosis of GC, so it can be regarded as the important means for early diagnosis.

• 한국축산식품학회지
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• 제34권5호
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• pp.717-723
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• 2014

A rapid and specific PCR assay for the simultaneous detection of Listeria monocytogenes, Escherichia coli O157:H7, Bacillus cereus, Salmonella spp., and Staphylococcus aureus in foods was developed to reduce the detection time and to increase sensitivity. Multiplex PCR developed in this study produced only actA, fliC, hbl, invA, ileS amplicons, but did not produce any non-specific amplicon. The primer sets successfully amplified the target genes in the multiplex PCR without any non-specific or additional bands on the other strains. The multiplex PCR assays also amplified some target genes from five pathogens, and multiplex amplification was obtained from as little as 1 pg of DNA. According to the results from the sensitivity evaluation, the multiplex PCR developed in this study detected 10 cells/mL of the pathogens inoculated in milk samples, respectively. The results suggested that multiplex PCR was an effective assay demonstrating high specificity for the simultaneous detection of five target pathogens in food system.

• Journal of Radiation Protection and Research
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• 제21권3호
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• pp.167-173
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• 1996

알파선 검출 효율 측정을 위한 고체비적검출기의 교정 방법을 제시하였으며, 제시된 교정 방법을 이용하여 CN-85와 LEXAN의 검출 효율을 측정하였다. $0{\sim}5.5MeV$ 에너지 영역의 알파선에 대한 검출 효율을 측정한 결과 CN-85와 LEXAN이 각각 97%, 57%였다. CN-85가 LEXAN에 비해 검출 효율이 높게 나타난 이유는 알파선에 대한 CN-85의 sensitivity가 LEXAN에 비해 좋을 뿐만 아니라 에너지 의존성이 CN-85가 LEXAN에 비해 낮기 때문인 것으로 분석되었으며, CN-85는 알파선의 유도 에너지가 약 3 MeV, LEXAN은 약 1.8 MeV에서 검출 효율이 최대로 나타났다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권6호
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• pp.413-417
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• 2000

In this study, a direct detection system for triazine derivative herbicides was developed using the photosynthetic reaction center (RC) from the purple bacterium, Rhodobacter sphaeroides, and surface plasmon resonance (SPR) apparatus. The histidine-tagged RCs were immobilized on an SPR gold chip using nickel-nitrilotriacetic acid groups as a binder for one of the triazine herbicide, atrazine. The SPR responses were proportional to the sample concentrations of atrazine in the range 0.1-1 $\mu\textrm{g}$/mL. The sensitivity of the direct detection of atrazine using the RC-assembled sensor chip was higher than that using the antibody-immobilized chip. The other types of herbicides, DCMU or MCPP, were not detected with such high sensitivity. The results indicated the high binding selectivity of the RC complex.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2010년도 춘계학술대회 초록집
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• pp.243.1-243.1
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• 2010

Devices based on nanomaterials platforms are emerging as a powerful tool for ultrasensitive sensors for the direct detection of biological and chemical species. In this talk, we will report the preparation and the full characterization of electrochemical polymerization of biopolymers platforms and nano-structure formation for electrochemical detection of enzymatic activity and toxic compound in electrolyte for biosensor applications. Formation of an electroactive polymer film of two different compounds has been quantified by observing new redox peak at higher potentials in cyclic voltammogram measurements. RCT value of at various biopolymer concentration based hybrid films has been obtained from electrochemical impedance spectroscopy analysis and possible mechanism for formation of complexes during electrochemical polymerization on conducting substrates has been investigated. Biosensors developed based on these hybrid biopolymers have very high sensitivity.

• 한국항공운항학회지
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• 제18권1호
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• pp.51-57
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• 2010

This study was to analyze the difference in pilot error by using the Signal Detection Theory. The task was to detect the targeted aircraft(signal) which is different shape from many other aircraft(noise). From the two experiments, we differentiated the task difficulty followed by change in noise stimuli. Experiment 1 was to search the signal stimuli(fighter plane) while the noise stimuli(cargo plane) were increasing. The results from the Experiment 1 showed the tendency to decrease the hit rate by increasing the number of noise stimuli. However, the false alarm rate was not increased. The sensitivity(d') showed quite high. In Experiment 2, a disturbance stimulus(helicopter) was added to noise stimuli. The result was generally similar to those of Experiment 1. However, the hit rate was lower than that of Experiment 1.