• 제목/요약/키워드: Detection agent

검색결과 461건 처리시간 0.03초

Screening and Evaluation of Streptomyces Species as a Potential Biocontrol Agent against a Wood Decay Fungus, Gloeophyllum trabeum

  • Jung, Su Jung;Kim, Nam Kyu;Lee, Dong-Hyeon;Hong, Soon Il;Lee, Jong Kyu
    • Mycobiology
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    • 제46권2호
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    • pp.138-146
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    • 2018
  • Two-hundred and fifty-five strains of actinomycetes isolated from soil samples were screened for their antagonistic activities against four well-known wood decay fungi (WDF), including a brown rot fungus, Gloeophyllum trabeum and three white rot fungi Donkioporia expansa, Trametes versicolor, and Schizophyllum commune. A dual culture assay using culture media supplemented with heated or unheated culture filtrates of selected bacterial strains was used for the detection of their antimicrobial activity against four WDF. It was shown that Streptomyces atratus, S. tsukiyonensis, and Streptomyces sp. greatly inhibited the mycelial growth of the WDF tested compared with the control. To evaluate the biocontrol efficacy of S. atratus, S. tsukiyonensis, and Streptomyces sp., wood blocks of Pinus densiflora inoculated with three selected Streptomyces isolates were tested for weight loss, compression strength (perpendicular or parallel to the grain), bending strength, and chemical component changes. Of these three isolates used, Streptomyces sp. exhibited higher inhibitory activity against WDF, especially G. trabeum, as observed in mechanical and chemical change analyses. Scanning electron microscopy showed that cell walls of the wood block treated with Streptomyces strains were thicker and collapsed to a lesser extent than those of the non-treated control. Taken together, our findings indicate that Streptomyces sp. exhibits the potential to be used as a biocontrol agent for wood decay brown rot fungus that causes severe damage to coniferous woods.

소화약제 누기 감시장치의 모듈개발 및 성능검증에 관한 연구 (A Study on the Development of a Fire Extinguishing Agent Leakage Monitoring Module and its Performance Assessment)

  • 손봉세;홍성호;고아라
    • 한국화재소방학회논문지
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    • 제30권2호
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    • pp.43-48
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    • 2016
  • 가스계소화설비는 소화약제의 자연 누기와 소화설비 노후로 인한 누기 등으로 화재진압 능력이 저하되는 문제점이 발생하고 있다. 이에 본 연구에서는 소화약제의 누기를 실시간으로 감지하는 압력누기 체크센서 모듈을 개발하였으며, 개발 모듈에 대한 성능평가를 하였다. 국내 외적으로 압력누기 감시 시스템에 대한 명확한 시험기준이 마련되어 있지 않다. 따라서 국외 유사 기준 ISO 7240, FM 1421와 국내 수신기 형식승인 및 제품검사의 기술기준을 참고하여 개발 모듈에 맞게 기본 성능과 내환경성에 대한 기준을 마련하고 평가를 실행하였다. 기본 성능 평가는 압축공기를 모듈에 인가하는 방식으로 최저작동 압력이 0.3 bar인 것을 확인하였다. 내환경성 검증은 통상적으로 발생할 수 있는 기후변화에도 오작동 없이 정상적으로 작동하는지를 확인하였다. 그 결과, 고온($50^{\circ}C$) 및 저온($-10^{\circ}C$) 환경 기준에서 이상 없이 정상 작동하였다.

Polymeric nanoparticles as dual-imaging probes for cancer management

  • Menon, Jyothi U.;Jadeja, Parth;Tambe, Pranjali;Thakore, Dheeraj;Zhang, Shanrong;Takahashi, Masaya;Xie, Zhiwei;Yang, Jian;Nguyen, Kytai T.
    • Biomaterials and Biomechanics in Bioengineering
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    • 제3권3호
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    • pp.129-140
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    • 2016
  • This article reports the development of biodegradable photoluminescent polymer (BPLP)-based nanoparticles (NPs) incorporating either magnetic nanoparticles (BPLP-MNPs) or gadopentate dimeglumine (BPLP-Gd NPs), for cancer diagnosis and treatment. The aim of the study is to compare these nanoparticles in terms of their surface properties, fluorescence intensities, MR imaging capabilities, and in vitro characteristics to choose the most promising dual-imaging nanoprobe. Results indicate that BPLP-MNPs and BPLP-Gd NPs had a size of $195{\pm}43nm$ and $161{\pm}55nm$, respectively and showed good stability in DI water and 10% serum for 5 days. BPLP-Gd NPs showed similar fluorescence as the original BPLP materials under UV light, whereas BPLP-MNPs showed comparatively less fluorescence. VSM and MRI confirmed that the NPs retained their magnetic properties following encapsulation within BPLP. Further, in vitro studies using HPV-7 immortalized prostate epithelial cells and human dermal fibroblasts (HDFs) showed > 70% cell viability up to $100{\mu}g/ml$ NP concentration. Dose-dependent uptake of both types of NPs by PC3 and LNCaP prostate cancer cells was also observed. Thus, our results indicate that BPLP-Gd NPs would be more appropriate for use as a dual-imaging probe as the contrast agent does not mask the fluorescence of the polymer. Future studies would involve in vivo imaging following administration of BPLP-Gd NPs for biomedical applications including cancer detection.

Development of a Ubiquitous Vision System for Location-awareness of Multiple Targets by a Matching Technique for the Identity of a Target;a New Approach

  • Kim, Chi-Ho;You, Bum-Jae;Kim, Hag-Bae
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2005년도 ICCAS
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    • pp.68-73
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    • 2005
  • Various techniques have been proposed for detection and tracking of targets in order to develop a real-world computer vision system, e.g., visual surveillance systems, intelligent transport systems (ITSs), and so forth. Especially, the idea of distributed vision system is required to realize these techniques in a wide-spread area. In this paper, we develop a ubiquitous vision system for location-awareness of multiple targets. Here, each vision sensor that the system is composed of can perform exact segmentation for a target by color and motion information, and visual tracking for multiple targets in real-time. We construct the ubiquitous vision system as the multiagent system by regarding each vision sensor as the agent (the vision agent). Therefore, we solve matching problem for the identity of a target as handover by protocol-based approach. We propose the identified contract net (ICN) protocol for the approach. The ICN protocol not only is independent of the number of vision agents but also doesn't need calibration between vision agents. Therefore, the ICN protocol raises speed, scalability, and modularity of the system. We adapt the ICN protocol in our ubiquitous vision system that we construct in order to make an experiment. Our ubiquitous vision system shows us reliable results and the ICN protocol is successfully operated through several experiments.

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IEC1131-3 표준언어 처리를 위한 지능적 소프트웨어 PLC 개발 (Development of an Intelligent Software Programmable Logic Controller for IEC1131-3 International Standard Languages)

  • 조영임
    • 한국지능시스템학회논문지
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    • 제14권2호
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    • pp.207-215
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    • 2004
  • IEC1131-3의 PLC(Programmable Logic Controller) 프로그래밍 언어는 프로그래밍이 복잡하여 디버깅이 어려우며 범용성이 없을 뿐 아니라 국내는 PC기반 소프트웨어 PLC 연구개발이 매우 미약하다. 따라서 본 논문은 국제 PLC 표준언어로 제정된 5가지 언어 중 국내에서 90%이상을 사용하고 있는 PLC 언어인 LD언어에 대한 표준규격을 연구하고, LD를 기존 상용화된 편집기(Visual C++)에서 활용 가능한 C코드로 변환하여 LD에 익숙한 사용자나 고급언어에 익숙한 사용자 모두 사용할 수 있는 지능적 에이전트 기반의 통합 시스템 ISPLC(Intelligent Agent System based Software Programmable Logic Controller)를 개발하였다. ISPLC에서는 LD에서보다 C에서 논리오류 검출기능이 훨씬 효율적이며, GUI 기반 인터페이스를 제공하며 에이전트에 의한 프로그래밍 코드를 제공한다. ISPLC는 초보자는 물론 PLC에 익숙한 사용자들에게도 효율적인 프로그래밍 플랫폼을 제공한다. 이러한 LD에서 IL로, IL에서 C로의 코드변환체제에 관한 연구는 국내외적으로 처음 시도되는 연구이다. ISPLC를 실제 실시간 교통량 제어 시스템에 적용하여 시뮬레이션한 결과 ISPLC가 오류검색 뿐 아니라 프로그래밍 시간을 기존 소프트에어 PLC에 비해 단축시켜줌을 알 수 있었다.

NAC 의 post-connect에서 행위정보를 사용한 악성코드 감염 호스트 탐지 시스템 (The Detection System for Hosts infected Malware through Behavior information of NAC post-connect)

  • 한명묵;선종현
    • 컴퓨터교육학회논문지
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    • 제13권6호
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    • pp.91-98
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    • 2010
  • NAC(Network Access Control)는 운영체제 보안 패치 미 적용 혹은 AV(Anti-Virus)미설치 컴퓨터 등 웜의 공격대상이 되어 내부망에 바이러스를 유포하는 엔드 포인트 사용자 보안에 대한 솔루션으로 개발되었다. 현재 시장에 상용화된 NAC 제품들이 경우 연결 전 보안기능(pre-connect)기술들은 많이 발전되어 있으나, 정상적인 인증을 통해 연결된 이후에 발생하는 위협을 탐지하는 위협 관리 기능(post-connect)이 대체적으로 부족한 상태이며, 이에 따라 Zero-day 공격, 악성코드 감염 등으로 NAC 사용자들이 지속적으로 피해를 입고 있는 상황이다. 본 논문에서는 이러한 post-connect단계에서의 문제점을 해결하고자 기존에 사용되던 단말에 대한 인증과 정책 위반 여부 검사 외에 각 단말이 발생시키는 트래픽 정보와 Agent를 통해 획득한 각 단말의 정보, 그리고 Network Scanner에서 획득한 Open Port와 네트워크 구성 변경 정보를 활용하여 정상 Behavior profile을 생성하고 이를 기반으로 악성코드 감염 시스템을 탐지하는 시스템을 제안한다.

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고속액체크로마토그라피에 의한 계란중의 합성항균제 잔류량 (Residue of Synthetic Antimicrobial Agent in Eggs by High Performance Liquid Chromatography)

  • 김종배;이성국;김두희
    • 농촌의학ㆍ지역보건
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    • 제18권1호
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    • pp.55-63
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    • 1993
  • 고속액체크로마토그라피를 이용하여 계란 중의 합성항균제 설파메라진, 설파메타진, 설파디메톡신, 후라졸리돈, 죠렌 등의 잔류량에 관한 신속하고 간편한 동시분서법을 검토하였는데, 각 합성항균제 표준용액을 첨가하여 나타난 회수율은 설파메라진 81.2%, 설파메타진 87.6%, 설파디메톡신 92.5%, 후라졸리돈 86.1%, 죠렌 73.9%로 나타났으며, 검출한계는 설파메라진, 설파메타진, 설파디메톡신이 0.2ppb 수준으로 나타났고, 후라졸리돈과 죠렌 0.5ppb 수준으로 나타났다. 본 분석방법으로 대구지역 시판계란 84개에 대한 잔류량을 조사한 결과, 설파메타진 3개 계란, 설파디메톡신이 4개 계란에서 각각 0.005-0.008ppm과 0.012-0.019ppm 정도 검출되었으며, 설파메라진, 후라졸리돈, 죠렌은 모든 시료에서 검출되지 않았다. 이상의 결과로 추정해 볼 때 설파메라진, 설파메타진, 설파디메톡신, 후라졸리돈, 죠렌의 계란내 잔류정도는 비교적 안전한 수준으로 나타났다.

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Development of HPTLC Fingerprinting and Phytochemical Study of a Polyherbal Unani Formulation

  • Alam, Abrar;Siddiqui, Javed Inam;Naikodi, Mohammed Abdul Rasheed;Kazmi, Munawwar Husain
    • 셀메드
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    • 제10권1호
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    • pp.7.1-7.6
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    • 2020
  • Plants produce a wide range of active principles, making them a rich source of different types of medicines. Without any knowledge of the phytoconstituents or active principles the medicinal plants itself or in the form of polyherbal formulations, were used by all society of human being from ancient times for prevention and cure of disease, but most of the traditional formulations including the formulation of Ayurveda and Unani have not been scientifically validated in order to establish the pharmacopoeial standards to improve the efficacy. Globally, the people become conscious that uses of synthetic drugs for a long period is not safe; the trend of medical society at large is looking at alternatives from natural, safe sources to combat diseases. Due to this comprehension, it has been increased the demand for plant-derived medicine, and on the other side, it is extremely important to standardize the polyherbal formulations and validate them scientifically to improve their safety and efficacy. The polyherbal Unani formulation Safuf-e-Muallif is widely used and recommended in Unani system of medicine (USM) as a spermatogenic agent, semen thickening agent, etc. to treat sexual disorders viz. premature ejaculation, nocturnal emission, etc. The study mainly deals with phytochemical screening for the detection of nature of phytoconstituents and analytical technique like High-performance thin-layer chromatography (HPTLC) for developing fingerprint of Safuf-e-Muallif revealing specific identities of the drug. The phytochemical screening and HPTLC fingerprint profile for SM reported here may be used as a reference standard for quality control purpose in future.

Polymeric Precursor법에 의한 LaMeO3 (Me = Cr, Co)의 제조 및 NOx 가스 검지 특성 (Fabrication and NOx Gas Sensing Properties of LaMeO3 (Me = Cr, Co) by Polymeric Precursor Method)

  • 이영성;;송정환
    • 한국재료학회지
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    • 제21권8호
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    • pp.468-475
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    • 2011
  • [ $LaMeO_3$ ](Me = Cr, Co) powders were prepared using the polymeric precursor method. The effects of the chelating agent and the polymeric additive on the synthesis of the $LaMeO_3$ perovskite were studied. The samples were synthesized using ethylene glycol (EG) as the solvent, acetyl acetone (AcAc) as the chelating agent, and polyvinylpyrrolidone (PVP) as the polymer additive. The thermal decomposition behavior of the precursor powder was characterized using a thermal analysis (TG-DTA). The crystallization and particle sizes of the $LaMeO_3$ powders were investigated via powder X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), and particle size analyzer, respectively. The as-prepared precursor primarily has $LaMeO_3$ at the optimum condition, i.e. for a molar ratio of both metal-source (a : a) : EG (80a : 80a) : AcAc (8a) inclusive of 1 wt% PVP. When the as-prepared precursor was calcined at $700^{\circ}C$, only a single phase was observed to correspond with the orthorhombic structure of $LaCrO_3$ and the rhombohedral structure of $LaCoO_3$. A solid-electrolyte impedance-metric sensor device composed of $Li_{1.5}Al_{0.5}Ti_{1.5}(PO_4)_3$ as a transducer and $LaMeO_3$ as a receptor has been systematically investigated for the detection of NOx in the range of 20 to 250 ppm at $400^{\circ}C$. The sensor responses were able to divide the component between resistance and capacitance. The impedance-metric sensor for the NO showed higher sensitivity compared with $NO_2$. The responses of the impedance-metric sensor device showed dependence on each value of the NOx concentration.

엔진윤활유 중 Ethylene Glycol의 용제추출후 bistrimethylsilyltrifluoroacetamide(BSTFA)를 이용한 GC/MS 분석에 관한 연구 (GC/MS Analysis of Ethylene Glycol in the Contaminated Lubricant Oil Through Solvent Extraction Followed by Derivatization using Bistrimethylsilyltrifluoroacetamide (BSTFA))

  • 이준배;권오성;유재훈;손성건;성태명;팽기정
    • Tribology and Lubricants
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    • 제28권6호
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    • pp.315-320
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    • 2012
  • For proper functioning, general machines usually need lubricant oil as a cooling, cleaning, and sealing agent at points of mechanical contact. The quality of lubricant oil can deteriorate during operation owing to various causes such as high temperature, combustion products and extraneous impurities. In this study, a heavy load stopped during operation, and the oil was analyzed to check whether any impurities were added. Extraction using acetonitrile followed by reaction with BSTFA(bistrimethylsilyl trifluoroacetamide) showed that, trimethylsilylated ethylene glycol was present in the lubricant oil. To quantify the ethylene glycol in the oil, deuterium-substituted ethylene glycol, which acted as an internal standard, was added to the sample and then extracted with the solvent. Next, the extract was reacted with the derivatizing agent(BSTFA) and then analyzed with GC/MS. The detection limit of this method was found to be $0.5{\mu}g/g$ and the recovery of oil containing $20,000{\mu}g/g$ of ethylene glycol was measured to be 94.8%. A damaged O-ring and eroded cylinder liner were found during the overhaul, which implied the leakage of coolant containing ethylene glycol into the lubricating system. The erosion of the cylinder liner was assumed to be due to cavitation of the coolant in the cooling system.