• 제목/요약/키워드: Desulfovibrio

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Precipitation of Soluble Uranium in Anaerobic Culture of Desulfovibrio desulfricans (황산환원균 Dseulfovibrio desulfricans의 혐기배양에 의한 가용성 우라늄의 침전)

  • 조규성;주현규;다야마사히또;도네세쯔지;홍고야쓰히로
    • Microbiology and Biotechnology Letters
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    • v.21 no.3
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    • pp.269-275
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    • 1993
  • The aims of the present study are to examine the precipitation of uranyl ion in the culture of Desulfovibrio desulfricans for the sedimentary recovery of aqueous uranium. D. desulfricans had the highest utilization rate of lactate and precipitated iron ion in the three sulfate reducing bacteria. So, subsequent experiments were conducted using lactate as an energy source. The normal growth was observed with increased pH and lactate utilization. During the culture, the amounts of SO42- consumed and S2- produced in aqueous phase were 8.5 and 7.5 mmol/m3-broth, respectively.

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Partial Assignment of Heme Groups of Cytochrome $c_3$ of Desulfovibrio vulgaris Miyazaki F by $^{1}H$-NMR

  • Jang-Su Park;Shin Won Kang
    • Bulletin of the Korean Chemical Society
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    • v.14 no.5
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    • pp.588-592
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    • 1993
  • The $^1H-NMR$ signals of the heme methyl, propionate and related chemical groups of cytochrome $c_3$ from Desulfovibrio vulgaris Miyazaki F (D.v. MF) were site-specifically assigned by means of 1D-NOE, 2D-DQFCOSY and 2D-TOCSY spectra. They were consistent with the site-specific assignments of the hemes with the highest and second-lowest redox potentials reported by Fan et al. (Biochemistry, 29, 2257-2263 1990). The site-specific heme assignments were also supported by NOE between the methyl groups of these hemes and the side chain of Val-18.

Change of Sponge(Axinella sp.)-Associated Bacterial Community during the Cultivation with Hexabromobenzene (Hexabromobenzens 농후 배양에 따른 해면(Axinella sp.) 공생 미생물의 군집구조 변화)

  • Seo, Hyun-Seok;Yang, Sung-Hyun;Bae, Seung Seob;Lee, Jung-Hyun;Kwon, Kae Kyoung
    • Journal of Marine Bioscience and Biotechnology
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    • v.6 no.2
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    • pp.76-83
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    • 2014
  • Bacteria associated with marine sponges seemed to be concerned in halogenation/dehalogenation process of natural compounds. In the present study, the effect of hexabromobenzene (HBB) on the community structure of bacteria associated with a marine sponge Axinella sp. from Chuuk State under anaerobic condition was investigated. Regardless of 100 ppm HBB, most of detected microorganisms displayed high similarity with clones reported from coral or sponges. Amongst, Desulfovibrio marinisediminis like clones were dominant. Clones affiliated with Lentisphaerae and Fusibacter paucivorans (Clostridia) were detected at the conditions without HBB but clones affiliated with Vallitalea guaymasensis (Clostridia) increased its proportion with HBB. From these results and previous reports clones affiliated with D. marinisediminis and V. guaymasensis seemed to be concerned in halogenation/dehalogenation process.

Metal Corrosion Mechanism by Sulfate-reducing and Iron-oxidizing Bacteria in Saline System and its Optimal Inactivation (염수계 철산화균 및 황환원균에 의한 금속 부식 및 최적 제어 방안)

  • Sung, Eun-Hae;Han, Ji-Sun;Kim, Chang-Gyun
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.8
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    • pp.798-807
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    • 2008
  • Due to economic impairment derived from metal corrosion of pumping station installed around coastal area, it was needed for related cause-effect to be investigated for understanding practical corrosion behavior and providing proper control. This research was thus carried out to determine whether the microbe can influence on metal corrosion along with its control in the laboratory. For this study, groundwater was sampled from the underground pump station(i.e. I Gas Station) where corrosion was observed. Microbial diversity on the samples were then obtained by 16S rDNA methods. From this, microbial populations showing corrosion behaviors against metals were reported as Leptothrix sp.(Iron oxidizing) and Desulfovibrio sp.(Sulfur reducing) Iron oxidizing bacteria were dominantly participating in the corrosion of iron, while sulfate reducing bacteria were more preferably producing precipitate of iron. In case of galvanized steel and stainless steel, iron oxidizing bacteria not only enhanced the corrosion, but also generated its scale of precipitate. Sulfate reducing bacteria had zinc steel corroded greater extent than that of iron oxidizing bacteria. In the inactivation test, chlorine or UV exposure could efficiently control bacterial growth. However as the inactivation intensity being increased beyond a threshold level, corrosion rate was unlikely escalated due to augmented chemical effect. It is decided that microbial corrosion could be differently taken place depending upon type of microbes or materials, although they were highly correlated. It could be efficiently retarded by given disinfection practices.

Isolation and Characterization of Sulfate- and Sulfur-reducing Bacteria from Woopo Wetland, Sunchun Bay, and Tidal Flat of Yellow Sea (우포늪, 순천만, 서해 갯벌에서부터 분리한 황산염/황-환원 세균의 특성 분석)

  • Kim, So-Jeong;Min, Ui-Gi;Hong, Heeji;Kim, Jong-Geol;Jung, Man-Young;Cha, In-Tae;Rhee, Sung-Keun
    • Korean Journal of Microbiology
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    • v.50 no.3
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    • pp.254-260
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    • 2014
  • Sulfur compound includes major electron acceptors for anaerobic respiration. In this study, cultivation-based study on sulfate- and sulfur-reducing bacteria of various wetlands of Korea was attempted. To isolate sulfate- and sulfur-reducing bacteria, anaerobic roll tube method was used to obtain typical black colonies of sulfate- and sulfur-reducing bacteria. Total 11 strains obtained were tentatively identified based on comparative 16S rDNA similarity and physiological property analysis. All sulfate-reducing bacteria (8 strains) belonged to genus Desulfovibrio with >99% 16S rDNA similarities. Three sulfur reducing bacteria were also isolated: two and one isolates were affiliated with Sulfurospirillum and Desulfitobacterium, respectively. These sulfate- and sulfur-reducing bacteria were able to utilize lactate and pyruvate and sulfite and thiosulfate as common electron donors and electron acceptors, respectively. This case study will provide fundamental information for obtaining useful indigenous sulfate- and sulfur-reducing bacteria from Korean wetlands employing various combinations of cultivation conditions.