• Animal Bioscience
• /
• v.35 no.12
• /
• pp.1850-1859
• /
• 2022

Objective: The quantitative reverse transcription polymerase chain reaction (qPCR) is the most accurate and reliable technique for analysis of gene expression. Endogenous reference genes (RGs) have been used to normalize qPCR data, although their expression may vary in different tissues and experimental conditions. Verification of the stability of RGs in selected samples is a prerequisite for reliable results. Therefore, we attempted to identify the most stable RGs in the hypothalamic-pituitary-gonadal (HPG) axis in sows. Methods: The cycle threshold values of nine commonly used RGs (18S, HPRT1, GAPDH, RPL4, PPIA, B2M, YWHAZ, ACTB, and SDHA) from HPG axis-related tissues in the domestic sows in the different stages of estrus cycle were analyzed using two RG-finding programs, geNorm and Normfinder, to rank the stability of the pool of RGs. In addition, the effect of the most and least stable RGs was examined by normalization of the target gene, gonadotropin-releasing hormone (GnRH), in the hypothalamus. Results: PPIA, HPRT1, and YWHAZ were the most stable RGs in the HPG axis-related tissues in sows regardless of the stages of estrus cycle. In contrast, traditional RGs, including 18S and ACTB, were found to be the least stable under these experimental conditions. In particular, in the normalization of GnRH expression in the hypothalamus against several stable RGs, PPIA, HPRT1, and YWHAZ, could generate significant (p<0.05) elevation of GnRH in the preovulatory phase compared to the luteal phase, but the traditional RGs with the least stability (18S and ACTB) did not show a significant difference between groups. Conclusion: These results indicate the importance of verifying RG stability prior to commencing research and may contribute to experimental design in the field of animal reproductive physiology as reference data.

• Journal of the Korean Geotechnical Society
• /
• v.25 no.9
• /
• pp.57-66
• /
• 2009

In the preliminary investigation (Park et al., 2009), the use of compressional wave velocity and its measurement techniques were proposed as a new quality control measure for trackbed fills. The methodology follows exactly the same procedure as the density control, except the density being replaced by the compressional wave velocity involving consistently with resilient modulus of design stage. The specifications for the control also include field compaction water content of optimum moisture content ${\pm}2%$ as well as the compressional wave velocity. In this sequel paper, crosshole and resonant column tests were performed as well direct-arrival method and laboratory compressional wave measurements to verify the practical applicability of a methodology far the new quality control procedure based upon compressional wave velocity. The stress-modified crosshole results reasonably well agree with the direct-arrival values, and the resonant column test results also agree well with the field crosshole results. The compressional wave velocity turned out to be an excellent control measure for trackbed fills both in the theoretical and practical point of view.

• Steel and Composite Structures
• /
• v.47 no.1
• /
• pp.91-102
• /
• 2023

To study the evaluation standard and control limit of mortar filling layer void length, in this paper, the train sub-model was developed by MATLAB and the track-bridge sub-model considering the mortar filling layer void was established by ANSYS. The two sub-models were assembled into a train-track-bridge coupling dynamic model through the wheel-rail contact relationship, and the validity was corroborated by the coupling dynamic model with the literature model. Considering the randomness of fastening stiffness, mortar elastic modulus, length of mortar filling layer void, and pier settlement, the test points were designed by the Box-Behnken method based on Design-Expert software. The coupled dynamic model was calculated, and the support vector regression (SVR) nonlinear mapping model of the wheel-rail system was established. The learning, prediction, and verification were carried out. Finally, the reliable probability of the amplification coefficient distribution of the response index of the train and structure in different ranges was obtained based on the SVR nonlinear mapping model and Latin hypercube sampling method. The limit of the length of the mortar filling layer void was, thus, obtained. The results show that the SVR nonlinear mapping model developed in this paper has a high fitting accuracy of 0.993, and the computational efficiency is significantly improved by 99.86%. It can be used to calculate the dynamic response of the wheel-rail system. The length of the mortar filling layer void significantly affects the wheel-rail vertical force, wheel weight load reduction ratio, rail vertical displacement, and track plate vertical displacement. The dynamic response of the track structure has a more significant effect on the limit value of the length of the mortar filling layer void than the dynamic response of the vehicle, and the rail vertical displacement is the most obvious. At 250 km/h - 350 km/h train running speed, the limit values of grade I, II, and III of the lengths of the mortar filling layer void are 3.932 m, 4.337 m, and 4.766 m, respectively. The results can provide some reference for the long-term service performance reliability of the ballastless track-bridge system of HRS.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
• /
• v.7 no.6
• /
• pp.55-62
• /
• 2017

This research analyzes the character factor in picture book, so the purpose is to develop the early childhood character program through Nuri curriculum. Picture book is the most familiar media for childhoods, and we are available it naturally in our lives. For this first, we analyze 6 elements(consideration, respect, cooperation, sharing, order, filial duty) in teacher's guide of the Nuri curriculum. Next, consulting the analyzed factors, the 12 picture books which will be utilized this program are chosen by the standard like literary, artistic, educational value. The chosen picture book devised total 12 times education plan to design each 2 times education plan in the factors for teacher's guide of Nuri curriculum. Lastly, it was finally developed through validation process by 2 teachers and 2 the professors of early childhood education. The developed program is needed later field application and It is required of the effectiveness verification after we apply 12 times activities on each age of childhoods for 3 to 5 years od in education field, so it may improve elements like consideration, respect, cooperaion, sharing, order, filial duty as childhoods experience this program personally. Also, this can provide it to teachers in childhood eucation field as study guidance plan.

• Journal of Korea Trade
• /
• v.27 no.2
• /
• pp.115-129
• /
• 2023

Purpose - This study empirically analyzes the consumer value of risk management associated with illegal, unreported, and unregulated (IUU) fishing of fishery products imported to Korea. The global regulatory paradigm for IUU fishing has shifted from production-centered to market-centered. As a result, changes in the international fisheries trade environment emphasizing "transparency" and "legality" of the production process are accelerating. Therefore, changes in the management systems of fishery products entering the country are also needed. Accordingly, this study estimated the consumer value for risk management of IUU fishing, targeting major fish species imported to Korea, and derived the feasibility of introducing related policies. Design/methodology - This study used the choice experiment as an analysis model to estimate consumers' willingness to pay for the "possibility to check for IUU fishing." The choice experiment assumes that the value of a good or service is composed of separable attributes and that the sum of the part-worth of these individual attributes becomes the total value. In this study, respondents were presented with profiles comprising three attributes (country of origin, price, and possibility of checking IUU fishing) and the levels of frozen poulp squid, the subject of the analysis. The participants were asked to select their preferred profile. The marginal willingness to pay for each attribute was derived from the results of the respondents' choices using conditional logit model estimates. Findings - There is a marked difference in utility based on the preference of the country of origin of fishery products among consumers. In addition, the utility of fishery products that have undergone IUU fishing verification was observed to be higher, with the utility marked to be higher for lower prices. Originality/value - Estimating the policy value of the risk management in IUU fishing of imported fisheries products in this study is a novel attempt that has never been conducted before. Several studies have been conducted to assess the risk of IUU fishing associated with the import of fishery products internationally. However, such studies are yet to be conducted in Korea. Instead, policies and studies have focused on issues related to complying with trading partners' legal and transparent standards for exporting fishery products. This study should be the beginning of more in-depth empirical and theoretical explorations to establish order in the domestic seafood market and respond to changes in international regulations on IUU fishing.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.29 no.5A
• /
• pp.467-475
• /
• 2009

Existing studies have suggested Universal Curve only for supplemental damping by damper. Therefore net damping has been determined by means of arithmetic summation between intrinsic, aero-damping of cable and supplemental damping of damper. However linear combination approach by means of the arithmetic summation is not enough theoretical background. So validity of this approach should be verified in order to design adequate cable-damper system by engineers. This study establishes governing differential equation which can consider intrinsic, aero-damping and supplemental damping as well. And also analysis method is solved by combination of muller method and successive iteration method. Consequently, this study succeeds in verification for validity of linear combination approach. As a result of this study, linear combination approach is limitedly effective in case of low stiffness and optimum damping coefficient of damper, short distance from support to damper, lower vibration mode, low aero-damping, and normal windy environment. Whereas this study will be effective in case of opposite conditions, and existing studies or linear combination approach occur to further error. Meaning of this study presents exact solution for net damping of cable-damper system, and verifies linear combination approach by means of the analysis method. In the future, if monitoring of optimum damping coefficient of a damper against aero-damping is feasible on time, algorithm of this study will be available for control of cable and semi-active damper system such as magneto-rheological damper.

• Journal of Service Research and Studies
• /
• v.11 no.2
• /
• pp.118-130
• /
• 2021

This study designs and validates a greenhouse complex environmental control algorithm with a multi-phase processing scheme that can combine and control actuators according to the degree of change in the greenhouse environment. The composite environmental control system is a system in which the complex environmental controller analyzes the information detected by sensors and operates appropriately actuators to maintain the crop growth environment. A composite environmental controller directs control devices driving actuators through a composite environmental control algorithm, which calculates the values necessary for the operation of the control devices. Most existing algorithms carry out control procedures on a single phase by iteration cycle, which can cause abnormal changes in the greenhouse environment due to errors in output. The proposed algorithm distributes control procedures over multiple phases: environmental control, environmental control, and device operation, and every iteration cycle, detects environmental changes in the environmental control phase first, and then combines control devices that can control the environment in the environmental control phase, and finally, performs the controls to derive the actuators in the device operation phase. The proposed algorithm is designed based on the analysis of the relationship between greenhouse environmental elements and control devices deriving actuators. According to verification analysis, the multi-phase processing scheme provides room to modify or supplement the setting value and enables the control devices to reflect changes in the associated environmental components.

• The Journal of Korean Academic Society of Nursing Education
• /
• v.29 no.2
• /
• pp.124-137
• /
• 2023

Purpose: This study's aim was to confirm the importance and performance of the clinical practice of nurses working in the general wards of a tertiary hospital and to analyze their educational needs. Methods: The study employed a descriptive research design, and a self-reported questionnaire was developed and used for nurses in a tertiary hospital. Data were collected from July 22 to July 29, 2022, analyzed by an independent t-test, paired t-test, and one-way ANOVA for the importance and performance of each clinical nursing practice according to general characteristics, and then a post hoc verification was performed by Scheffé's test. An Importance-Performance Analysis and Borich needs assessment model were used to analyze clinical nursing education needs. Results: Clinical nursing practice performance showed a significant difference according to length of total clinical career, time working in current department, and preceptor experience. According to the results of the Importance-Performance Analysis, neurological evaluation, and nursing intervention, artificial respirator and high-flow oxygen inhalation nursing, chemotherapy, emergency nursing, and cardiopulmonary resuscitation were identified as belonging to the "concentrate here" quadrant. All these items ranked in the top 10 in the Borich needs assessment model. Conclusion: Based on these results, the current education system should be reviewed, and short and long term education strategies based on educational needs should be established to strengthen the competence of nurses.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.28 no.1B
• /
• pp.11-20
• /
• 2008

The spillway type of small and midsize dams in Korea is almost overflow weir. To examine flood control capacity of overflow spillway, FLOW-3D was applied to Daesuho dam and analysis was focused on the discharge of dam spillway by changing weir shape. Overflow phases and discharges of linear labyrinth weir and curved labyrinth weir were compared with those of existing linear ogee weir. Hydraulic model experiment was performed to verify numerical result. Verification results showed that overflow behaviors and flow characteristics in the side channel by hydraulic model experiment and numerical simulation are well matched, and water surface elevation at side wall coincides with each other. When the reservoir elevation was increased up to design flood level, in case of the linear ogee weir the flow over the crest ran through smoothly in the side channel, whereas in cases of linear labyrinth weir and curved labyrinth weirs, the flow discharge was increased by 40 cms, and the flow over the weir crest, rotating counter-clockwise, was submerged in the side channel. The results of the water level-discharge curve revealed that labyrinth weir can increase discharge by 71% compared to the discharge of linear ogee weir at low reservoir elevation since it can have longer effective length. But as water surface elevation rises, the slope of water level-discharge curve of labyrinth weir becomes milder by submergence and nappe interference in the side channel.

• Journal of Biomedical Engineering Research
• /
• v.44 no.6
• /
• pp.363-376
• /
• 2023

According to the COVID-19, development of various medical software based on IoT(Internet of Things) was accelerated. Especially, interest in a central software system that can remotely monitor and control ventilators is increasing to solve problems related to the continuous increase in severe COVID-19 patients. Since medical device software is closely related to human life, this study aims to develop central monitoring system that can remotely monitor and control multiple ventilators in compliance with medical device software development standards and to verify performance of system. In addition, to ensure the safety and reliability of this central monitoring system, this study also specifies risk management requirements that can identify hazardous situations and evaluate potential hazards and confirms the implementation of cybersecurity to protect against potential cyber threats, which can have serious consequences for patient safety. As a result, we obtained medical device software manufacturing certificates from MFDS(Ministry of Food and Drug Safety) through technical documents about performance verification, risk management and cybersecurity application.The purpose of this study is to conduct a usability assessment to ensure that ergonomic design has been applied so that the ventilator central monitoring system can improve user satisfaction, efficiency, and safety. The rapid spread of COVID-19, which began in 2019, caused significant damage global medical system. In this situation, the need for a system to monitor multiple patients with ventilators was highlighted as a solution for various problems. Since medical device software is closely related to human life, ensuring their safety and satisfaction is important before their actual deployment in the field. In this study, a total of 21 participants consisting of respiratory staffs conducted usability test according to the use scenarios in the simulated use environment. Nine use scenarios were conducted to derive an average task success rate and opinions on user interface were collected through five-point Likert scale satisfaction evaluation and questionnaire. Participants conducted a total of nine use scenario tasks with an average success rate of 93% and five-point Likert scale satisfaction survey showed a high satisfaction result of 4.7 points on average. Users evaluated that the device would be useful for effectively managing multiple patients with ventilators. However, improvements are required for interfaces associated with task that do not exceed the threshold for task success rate. In addition, even medical devices with sufficient safety and efficiency cannot guarantee absolute safety, so it is suggested to continuously evaluate user feedback even after introducing them to the actual site.