• 한국콘텐츠학회논문지
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• 제16권5호
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• pp.96-103
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• 2016

본 연구는 초등학생을 대상으로 참여식 및 강의식 교육법의 구강보건교육이 치과공포지수에 미치는 영향을 알아보고, 치과공포를 줄일 수 있는 구강보건교육 방안을 마련하고자 하였다. 연구대상은 2014년 4월 D초등학교에 다니는 10세 아동으로, 의사소통이 가능하며 전신 질환이 없는 아동 총 40명을 대상으로 하였다. 본 연구는 구강보건교육을 시행하기 전, 각 교육군 연구대상자들에게 치과공포지수를 측정하기 위하여 자가기입 설문조사를 수행하였다. 그 후, 2주에 걸쳐 한 시간 가량의 구강보건교육을 총 2회 진행하였다. 참여식 교육군에는 소아치과에서 사용하는 행동조절방법인 체계적 탈감작법(Tell(말)-Show(시범)-Do(시행))을 적용하여 구강보건교육을 진행하였고, 강의식 교육군(대조군)에는 power point(PPT)를 이용한 교육방법으로 실시하였다. 2회 교육이 종료된 후, 각 군에서 교육 전 조사한 설문지와 동일한 설문지를 이용하여, 치과공포지수를 재측정하였다. 참여식 교육군에서는 교육 전 치과공포지수가 9.61점이었으나, 교육 후에 7.13점으로 유의하게 감소하였으나, 강의식 교육군은 교육 전 11.14점에서 교육 후 10.59점으로 조사되었다. 따라서, 참여식 교육방법을 적용하는 구강보건교육이 소아 청소년의 치과공포지수를 감소시키는데 효과적인 것으로 사료되었다.

• The Korean Journal of Physiology and Pharmacology
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• 제4권3호
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• pp.185-195
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• 2000

Vascular diseases are significant complications of diabetes mellitus (DM), and the endothelial cells may play a pivotal role in the development of vascular disease in DM. Endothelin-1 (ET-1) released from endothelium is a potent vasoconstrictor peptide and circulating level of ET-1 is increased in a variety of disease states. The purpose of this study was to determine the changes of responsiveness to ET-1 in DM, and we experimented on the changes in the ET-1-induced contraction, levels of nitrite and lipid peroxidation, and ET-1 immunoreactivity in aorta from streptozotocin-induced DM rats. DM was induced by single injection of streptozotocin (55 mg/kg, i.p.). The immunoreactive ET-1 levels in endothelial layer of thoracic aorta were much higher in DM rats than control rats. Nitrite in tissue homogenate was decreased and plasma nitrite was increased in DM rats. Malondialdehyde (MDA) was significantly increased in DM rats and cGMP was not significantly different between control and DM rats. ET-1 produced concentration- dependent contractile responses that are significantly attenuated in DM rats compared to controls. In the presence of selective $ET_A$ receptor antagonist BQ610, the maximum contraction was decreased and the concentration ratios for BQ610 yielded $pA_2$ values of 7.3 (slope, 0.65) in control rats, whereas BQ610 had no antagonistic effect on ET-1-induced contraction in DM rats. However, pretreatment with BQ788, an $ET_B$ receptor antagonist, maximum response was decreased and the dose-response curves for ET-1 were shifted to the right in both groups and $pA_2$ values were 7.9 and 7.7 (slope, 1.05 in control and DM rats), respectively. IRL 1620 and sarafotoxin S6c, $ET_B$ agonists, induced relaxation in control rats but not in DM rats. These results indicate that endothelial cell dysfunction and enhanced immunoreactivity of ET-1 have been found in DM rat and ET-1-induced contraction was attenuated in DM rat. These attenuated responses might be at least in part caused by the alteration of $ET_A$ receptor properties (e.g. desensitization), and partly related with an alteration in intracellular mechanism for contraction to ET-1.

• 디지털융복합연구
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• 제16권6호
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• pp.233-240
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• 2018

본 연구는 EMDR 통합 집단치료가 간호대학원생의 우울, 불안, 외상 후 스트레스, 및 자아존중감에 미치는 효과를 파악하기 위해 시도되었다. 본 연구는 유사 실험연구로서 비동등성 대조군 전후설계를 이용하였다. 연구대상자는 C 대학 석, 박사과정에 재학 중인 간호대학원생 50명(실험군=22명, 대조군=28명)이며, 실험군은 총 8주간 8회기의 EMDR-IGTP에 참여하였고, 대조군은 연구종료 후 희망자에 한하여 EMDR-IGTP을 제공하였다. 수집된 자료는 SPSS 24.0 프로그램을 이용하여 서술통계, ${\chi}^2-test$와 Independant t-test로 분석하였다. 본 연구의 결과, 8주 후 EMDR-IGTP에 참여한 실험군은 대조군에 비해 불안(t=-2.50, p<.05)과 자아존중감(t=20.78, p<.01)에서 통계적으로 유의하게 차이가 나타났다. 따라서 EMDR-IGTP은 간호대학원생의 불안 감소와 자아존중감 증진에 긍정적인 효과가 있음이 입증되었다. 추후 연구대상자를 확대하여 프로그램의 효과의 기전을 파악하는 후속 연구를 제언하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.910-910
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• 2005

• The Korean Journal of Physiology and Pharmacology
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• 제3권2호
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• pp.165-174
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• 1999

We explore the question of whether adenosine 5'-triphosphate (ATP) acts as an excitatory neurotransmitter in guinea-pig gastric smooth muscle. In an organ bath system, isometric force of the circular smooth muscle of guinea-pig gastric antrum was measured in the presence of atropine and guanethidine. Under electrical field stimulation (EFS) at high frequencies (＞20 Hz), NO-mediated relaxation during EFS was followed by a strong contraction after the cessation of EFS (a 'rebound-contraction'). Exogenous ATP mimicked the rebound-contraction. A known $P_{2Y}-purinoceptor$ antagonist, reactive blue 2 (RB-2), blocked the rebound-contraction while selective desensitization of $P_{2Y}-purinoceptor$ with ${\alpha},{\beta}-MeATP$ did not affect it. ATP and 2-MeSATP induced smooth muscle contraction, which was effectively blocked by RB-2 and suramin, a nonselective $P_2-purinoceptor$ antagonist. Particularly, in the presence of RB-2, exogenous ATP and 2-MeSATP inhibited spontaneous phasic contractions, suggesting the existence of different populations of purinoceptors. Both the rebound-contraction and the agonist-induced contraction were not inhibited by indomethacin. The rank orders of agonists' potency were 2-MeSATP > ATP ${ge}$ UTP for contraction and ${\alpha},{\beta}-MeATP\;{\ge}\;{\beta},{\gamma}-MeATP$ for inhibition of the phasic contraction, that accord with the commonly accepted rank order of the classical $P_{2Y}-purinoceptor$ subtypes. Electrical activities of smooth muscles were only slightly influenced by ATP and 2-MeSATP, whereas ${\alpha},{\beta}-MeATP$ attenuated slow waves with membrane hyperpolarization. From the above results, it is suggested that ATP acts as an excitatory neurotransmitter, which mediates the rebound-contraction via $P_{2Y}-purinoceptor$ in guinea-pig gastric antrum.

• The Korean Journal of Physiology and Pharmacology
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• 제17권3호
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• pp.223-228
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• 2013

The calcium-activated $K^+$ ($BK_{Ca}$) channel is one of the potassium-selective ion channels that are present in the nervous and vascular systems. $Ca^{2+}$ is the main regulator of $BK_{Ca}$ channel activation. The $BK_{Ca}$ channel contains two high affinity $Ca^{2+}$ binding sites, namely, regulators of $K^+$ conductance, RCK1 and the $Ca^{2+}$ bowl. Lysophosphatidic acid (LPA, 1-radyl-2-hydroxy-sn-glycero-3-phosphate) is one of the neurolipids. LPA affects diverse cellular functions on many cell types through G protein-coupled LPA receptor subtypes. The activation of LPA receptors induces transient elevation of intracellular $Ca^{2+}$ levels through diverse G proteins such as $G{\alpha}_{q/11}$, $G{\alpha}_i$, $G{\alpha}_{12/13}$, and $G{\alpha}s$ and the related signal transduction pathway. In the present study, we examined LPA effects on $BK_{Ca}$ channel activity expressed in Xenopus oocytes, which are known to endogenously express the LPA receptor. Treatment with LPA induced a large outward current in a reversible and concentration-dependent manner. However, repeated treatment with LPA induced a rapid desensitization, and the LPA receptor antagonist Ki16425 blocked LPA action. LPA-mediated $BK_{Ca}$ channel activation was also attenuated by the PLC inhibitor U-73122, $IP_3$ inhibitor 2-APB, $Ca^{2+}$ chelator BAPTA, or PKC inhibitor calphostin. In addition, mutations in RCK1 and RCK2 also attenuated LPA-mediated $BK_{Ca}$ channel activation. The present study indicates that LPA-mediated activation of the $BK_{Ca}$ channel is achieved through the PLC, $IP_3$, $Ca^{2+}$, and PKC pathway and that LPA-mediated activation of the $BK_{Ca}$ channel could be one of the biological effects of LPA in the nervous and vascular systems.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제27권4호
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• pp.278-305
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• 2016

Objective: The aim of this systematic literature review is to analyze the psychosocial interventions for children and adolescents after disasters. Methods: We conducted a review of the extant research literature from 1991 to 2015 via a comprehensive search of the MEDLINE, EMBASE, Cochrane CENTRAL, PubMed and PsyclNFO databases. The keywords employed in this research included: 'child', 'adolescent', 'youth', 'disaster', 'posttraumatic', 'psychosocial', 'therapy' and 'intervention'. The researchers followed the PRISMA guidelines. A total of 850 articles were screened for their eligibility and fifty-nine were found to meet the study criteria. The final data analysis was performed based on the disaster type, study design, type of intervention, sample size, age, school grade, number of sessions, setting of intervention delivery, providers, approach and parent involvement. Results: Countries worldwide have experienced various kinds of disasters, including earthquakes, hurricanes, vessel accidents, tornados, tsunamis, volcanic eruptions, war, fire, terrorism, and traffic accidents. The types of psychosocial intervention that were conducted after these disasters included: psychological first aid, psychological debriefing, psychoeducation, trauma focused cognitive behavior therapy, eye movement desensitization reprocessing, prolonged exposure therapy, group play therapy and arts therapy, project interventions, school-based interventions and web-based interventions. Conclusion: The findings of the systematic literature review suggest that an appropriate psychosocial intervention could be utilized as evidence-based mental health treatment for children and adolescents after disasters.

• Restorative Dentistry and Endodontics
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• 제20권2호
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• pp.508-526
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• 1995

This experiment was performed to study mechanisms of desensitization by chemical desensitizing agents in hypersensitive dentin and compare effects of these agents by measuring the activity of intradental nerves and observing their occluding aspects on dentinal tubules with SEM over time after application of chemical desensitizing agents to the exposed dentinal surfaces. Canines of adult cats weighing 2-3 kg were cross-sectioned at 1.5 mm from incisal apex, and the smear layer of the exposed dentinal susface was removed by 32 % $H_3PO_4$ for 15 sec. Chemical desensitizing agents such as 10% $SrCl_2$, 5% $KNO_3$ and 30% $K_2C_2O_4$, were applied to the exposed dentin surfaces for 2 minutes. Intradental nerve activity was measured immediately after application of the agents, at 15 minutes and at 30 minutes by stimulating with 4M NaCl. To compare occluding ability of desensitizing agents on dentinal tubules in vivo and in vitro, the structures of the exposed dentinal surfaces of nonvital and vital teeth were morphologically observed by SEM. The results obtained were as follows : 1. Intradental nerve activity was decreased immediately after the application of 10 % $SrCl_2$, 5% $KNO_3$ and 30% $K_2C_2O_4$. (p<0.01), among which 30% $K_2C_2O_4$. showed the highest desensitizing effect(p<0.01). 2. The immediately decreased intradental nerve activity after application of 10 % $SrCl_2$ and 5% $KNO_3$ was increased over time. 10% $SrCl_2$ and 5% $KNO_3$ showed no desensitizing effect respectively at 30 minutes and at 15 minutes after application. 3. The immediately decreased intradental nerve activity after application of 30 % $K_2C_2O_4$ was persistently continued during the period of observation (p<0.01). 4. Precipitates of $SrCl_2$ and $KNO_3$ were not noted on the exposed dentinal surfaces and within dentinal tubules by SEM examination. On the other hand, 30 % $K_2C_2O_4$ produced precipitates on the exposed dentinal surfaces and openings of dentinal tubules without any formed preciptates within dentinal tubules. 5. Ten percent $SrCl_2$, 5 % $KNO_3$ and 30 % $K_2C_2O_4$ showed no differences in their occluding aspects on dentinal tubules either in vivo or in vitro studies and either immediately following application or at 30 minutes. These results suggest that the desensitizing effect of $SrCl_2$ and $KNO_3$ is resulted from their reducing effect on the intradental nerve activity rather than from their precipitates' occluding the dentinal tubules. However, desensitizing effect of 30 % $K_2C_2O_4$, is probably resulted from its precipitates' occluding the openings of the dentinal tubules as well as from it's reducing effect on the intradental nerve actibity.

• Clinical and Experimental Emergency Medicine
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• 제3권3호
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• pp.175-180
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• 2016

Objective Combination of ${\beta}_1-adrenergic$ receptor (AR) blockade and ${\beta}_2-AR$ activation might be a potential novel therapy for treating heart failure. However, use of ${\beta}-AR$ agonists and/or antagonists in the clinical setting is controversial because of the lack of information on cardiac inotropic or chronotropic regulation by AR signaling. Methods In this study, we performed hemodynamic evaluation by examining force frequency response (FFR), Frank-Starling relationship, and response to a non-selective ${\beta}-AR$ agonist (isoproterenol) in hearts isolated from 6-month-old transgenic (TG) mice overexpressing ${\beta}_1-$ and ${\beta}_2-ARs$ (${\beta}_1-$ and ${\beta}_2-AR$ TG mice, respectively). Results Cardiac physiologic consequences of ${\beta}_1-$ and ${\beta}_2-AR$ overexpression resulted in similar maximal response to isoproterenol and faster temporary decline of positive inotropic response in ${\beta}_2-AR$ TG mice. ${\beta}_1-AR$ TG mice showed a pronounced negative limb of FFR, whereas ${\beta}_2-AR$ TG mice showed high stimulation frequencies with low contractile depression during FFR. In contrast, Frank-Starling relationship was equally enhanced in both ${\beta}_1-$ and ${\beta}_2-AR$ TG mice. Conclusion Hemodynamic evaluation performed in the present showed a difference in ${\beta}_1-$ and ${\beta}_2-AR$ signaling, which may be due to the difference in the desensitization of ${\beta}_1-$ and ${\beta}_2-ARs$.

• 한국심리학회지 : 문화 및 사회문제
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• 제26권3호
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• pp.283-301
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• 2020

한국사회에서는 인터넷 커뮤니티와 게시글 댓글 상에서 여성혐오 단어가 생성되고 확산되고 있다. 본 연구는 여성혐오 단어에 대한 노출 정도가 개인이 가지는 여성혐오 태도에 미치는 영향을 알아보기 위해 실시되었다. 연구 1에서는 설문을 통해 여성혐오 단어의 노출 정도(알고 있는 여성혐오 단어 수, 인터넷 댓글을 보는 정도)와 명시적 여성혐오 태도 간의 관계를 알아보았다. 그 결과 혐오단어 노출이 많을수록 명시적 여성혐오 태도는 감소하였는데 이는 남성에게서 더 두드러지게 나타났다. 이는 부정적 미디어 자극에 대한 반복적 노출로 인한 자극 둔감화로 설명될 수 있다. 연구 2에서는 실험을 통해 여성혐오 단어의 노출 여부를 조작하고 암묵적 연합검사를 통해 암묵적 여성혐오 태도 간의 관계를 알아보았다. 집단 간 분산 분석 결과 남성의 경우 여성에 비해 혐오단어에 노출될수록 암묵적 여성혐오 태도가 더 강한 것으로 나타났다. 본 연구의 결과는 여성혐오 태도에 있어 명시적 태도와 암묵적 태도 간의 차이를 보여주며, 혐오단어에 대한 동일한 수준의 노출 정도가 남성과 여성의 태도 변화에 미치는 영향이 다를 수 있음을 시사한다.