• Natural Product Sciences
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• v.29 no.2
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• pp.113-119
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• 2023

Descurainia sophia seeds methanol extract showed significant anti-influenza activity and we tried to isolate anti-influenza compounds from the D. sophia extract. D. sophia seeds were extracted with 80% methanol and fractionated with n-hexane, ethyl acetate, CHCl₃ and n-butanol. The anti-influenza activity of each fraction was assessed using sulforhodamine B (SRB) method in A549 cells, human-derived lung cancer cells. The ethyl acetate and CHCl₃ fractions showed the most potent anti-influenza activity. Seven compounds were isolated from CHCl₃ fraction and identified 1-decanol (1), 2-(3,4-dihydroxy-2-methylenebutoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (2), daucosterol (3), isorhamnetin (4), quercetin (5), sinapic acid (6), and helveticoside (7) by spectroscopic data such as UV, IR, ¹H-NMR, ¹³C-NMR and mass spectroscopy. Anti-influenza activities of isolated compounds were evaluated using SRB method in A549 cells. Compounds 3, 4 and 7 had significant anti-influenza activity in a dose-dependent manner.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.5
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• pp.17-35
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• 2011

The purpose of this study is to offer the raw data for management and conservation of national park by systematic and objective surveying and analysing the flora distributed in the Hwarang district, Gyeongju national park. The results are as follows. The vascular plants in this site were summarized as 396 taxa: 95 families, 272 genera, 351 species, 2 subspecies, 38 varieties and 5 forma. The rare plants designated by Korea Forest Service were 5 taxa: Exochorda serratifolia S.Moore, Potentilla discolor Bunge, Prunus yedoensis Matsum, Lysimachia coreana Nakai and Inula salicina var. asiatica Kitam.. The korean endemic plants were 6 taxa: Populus tomentiglandulosa T.B.Lee, Clematis trichotoma Nakai, Lespedeza maritima Nakai, Lysimachia coreana Nakai, Forsythia koreana (Rehder) Nakai and Weigela subsessilis (Nakai) L.H.Bailey. The specific plants by floristic region were 30 taxa: Asplenium sarelii Hk., Salix chaenomeloides Kimura, Lysimachia barystachys Bunge, Achillea alpina L., Celtis aurantiaca Nakai, Vitex negundo var. incisa (Lam.) C.B.Clarke and so forth. The plants with approval for delivering oversea were 6 taxa: Clematis trichotoma Nakai, Exochorda serratifolia S.Moore, Glycine soja Siebold & Zucc., Lespedeza maritima Nakai, Lysimachia coreana Nakai and Weigela subsessilis (Nakai) L.H.Bailey. The naturalized plants were 49 taxa: Bilderdykia dumetora (L.) Holub, Descurainia pinnata Britton, Oxalis corymbosa DC., Bidens frondosa L., Panicum dichotomiflorum Michx. and so forth. The invasive alien plants were 2 taxa: Ambrosia artemisiifolia L. and Aster pilosus Willd. In future, if we will survey the flora of whole Gyeongju national park, we will offer the help to establishing the conservation plan of ecosystem in Gyeongju national park.

• The Korea Journal of Herbology
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• v.34 no.3
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• pp.19-30
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• 2019

Objectives : This study is to propose the identification keys based on stereoscopic examination of 8 seed herbs in 2 categories (Phyllolobobii Semen (PS) with 4 Astragali Semen (AS), and 2 Lepidii seu Descurainiae Semen (LDS) with 1 Drabae Semen (DS)) which have difficulties in discrimination with visual observation. Methods : We reviewed the description of original plants and their medicinal parts from the literature. The original plants were collected, identified, confirmed as specimens, and compared to the samples distributed in the market. The first identification was made by visual observation, and insufficient points were supplemented by stereoscopic observation. Identification criteria were set by considering morphological characteristics of authentic herbs, percentage of adulterants, and distinction between authentic herbs and adulterants. Results : The original plants of PS and AS could be distinguished by upright or lying form of stem, color of flowers, number of leaflets, and presence of hair of fruits. LDS and DS could be distinguished by leaf arrangement on stem: radical or cauline, whole plants size, leaf division, color of flowers, and shape of fruits. The herbal medicines of PS and AS could be distinguished by seed surface pattern, size, and hardness. LDS and DS could be distinguished by size, shape, viscosity when chewed, and degree of mucous layer formation when soaked in water. Conclusions : This study suggests the identification keys of original plants and herbal medicines. Especially, since fine seed herbs are difficult to distinguish by visual observation, the stereoscope should be applied to the discrimination.

• Korean Journal of Plant Resources
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• v.21 no.2
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• pp.162-170
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• 2008

The purpose of this study was to suggest the raw data on establishing the domestic wetland conservation plan. The results of this study were as follows. The vascular plants were summarized as 201 taxa in Ahnshim wetland; 57 families, 154 genera, 179 species and 22 varieties. The rare and endangered plant designated by Korea Forest Service was Hydorcharis dubia. In the wetland plants, the emerged plants were showed Typha angustata, Beckmannia syzigachne, Zizania latifolia, Phragmites communis, Persicaria thunbergii, Oenanthe javanica and Veronica undulata, and the submerged plants were Potamogeton crispus, Vallisneria asiatica, Hydrilla verticillata, Ceratophyllum demersum and Myriophyllum verticillatum. And the floating leaved plants were confirmed Potamogeton distinctus, Hydrocharis dubia, Nelumbo nucifera and Nymphoides peltata, and the floating plants were Salvinia natans, Spirodela polyrhiza and Lemna paucicostata. The naturalized plants were 33 taxa; Avena fatua, Dactylis glomerata, Rumex crispus, Rumex conglomeratus, Amaranthus retroflexus, Lepidium apetalum, Tlaspi arvense, Descurainia pinnata, Potentilla supina, Vicia villosa, Robinia pseruo-acacia, Amorpha fruticosa, Trifolium repens, Medicago sativa, Ailanthus altissima, Euphorbia supina, Oenothera lamarckiana, Quamoclit angulata, Ipomoea purpurea, Solanum nigrum, Veronica perisca, Helianthus tuberosus, Ambrosia artemisifolia var. elatior, Xanthium strumarium, Erigeron annuus, Erigeron canadensis, Conyza sumatrensis, Senecio vulgaris, Bidens frondosa, Tagetes minuta, Taraxacum officinale, Taraxacum laevigatum and Sonchus oleraceus.

• The Korea Journal of Herbology
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• v.33 no.3
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• pp.25-35
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• 2018

Objectives : Lepidii seu Descurainiae Semen has been frequently adulterated with the seeds of several inauthentic plant species. However, the accurate identification of these plant seeds is very difficult. To develop a reliable genetic authentication tool for Lepidii seu Descurainiae Semen, we analyzed matK sequence. Methods : To obtain the matK sequences of plant materials, genomic DNA was extracted from 24 samples and PCR amplification was carried out using matK-AF/matK-8R universal primer set and matK-LDSF/matK-LDSR primer set. For identifying species-specific nucleotides and phylogenetic analysis, matK regions were sequenced and comparatively analyzed by the ClustalW and Maximum Likelihood method. Results : We developed a new primer set to amplify matK region in Lepidii seu Descurainiae Semen and closely related plant samples. From the comparative analysis of matK sequences, we identified species-specific marker nucleotides for D. sophia, L. apetalum, L. latifolium, E. cheiranthoides, E. macilentum, and D. nemorosa, respectively. Furthermore, phylogenetic analysis revealed clear classification depending on the species. These results indicated that the matK sequence obtained a new primer set in this study was useful to identify Lepidii seu Descurainiae Semen in species level. Conclusions : We developed a primer set and identified species-specific marker nucleotides enough to distinguish authentic Lepidii seu Descurainiae Semen and adulterants at the species level based on the matK sequences. These genetic tool will be useful to prevent adulteration and to standardize the quality of Lepidii seu Descurainiae Semen.