• 대한의생명과학회지
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• 제19권4호
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• pp.315-329
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• 2013

This study was conducted to evaluate the distribution, frequency, sensitization rate, and types of specific allergen in allergic patients. We analyzed allergens of 12,882 allergic patient's serum referred for Medical Laboratory using MAST Allergy Screen Test (Inhalation/food panel) from April, 2010 to March, 2011. Allergen reactivity were not detected in 833/12,882 (6.5%) allergic patients and the other allergic patients had two or more types of allergens of food and inhalant, respectively. In the distribution of the allergen food 73.4% (9,450/12,882 patients) was much higher than the pollen 26.6% (3,432/12,882 patients). The sensitization rate to each allergen showed garlic 26.7%, egg white 21.5% of food allergens, mugwort 24.9%, ragweed short 19.6% of pollen allergens, Candida albicans 10.5%, Alternaria spp. 7.9% of fungus allergens, and showed high sensitized rate to Dermatophagoides farinae 90.2%, Dermatophagoides pteronyssinus 77.8%, house dust 57% of indoor allergens, respectively. The specific allergens of food allergen, fungus allergen, and indoor allergen were more frequent in young child than adult, but pollen allergens were frequent in adult than young child. In seasonal distribution, the food allergen were frequent in summer and autumn, the pollen allergen in autumn, fungus allergen in spring and in winter, and indoor allergen in autumn and in winter. In conclusion, the mite of Dermatophagoides farinae and Dermatophagoides pteronyssinus, and house dust showed the highest sensitization rate in specific allergen of allergic patients. The allergens showed difference feature according to the age, region, and types of allergen.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1591-1596
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• 2006

Acaricidal effects of materials derived from Caesalpinia sappan heartwoods against Dermatophagoides farinae and D. pteronyssinus were assessed and compared with those evidenced by commercial benzyl benzoate and DEET. The observed responses varied according to dosage and mite species. The $LD_{50}$ values of the methanol extracts derived from C. sappan heartwoods were 6.13 and $5.44{\mu}g/cm^3$ against D. farinae and D. pteronyssinus, respectively. Furthermore, the ethyl acetate fraction derived from the methanol extract was approximately 8.71 more toxic than DEET against D. farinae, and 4.73 times more toxic against D. pteronyssinus. The biologically active constituent from the ethyl acetate fraction of C. sappan heartwood extract was purified via silica gel chromatography and high-performance liquid chromatography. The structure of the acaricidal component was analyzed by $GC-MS,\;^1H-NMR,\;^{13}C-NMR,\;^1H-^{13}C\;COSY-NMR$, and DEPT-NMR spectroscopy, and identified as juglone (5-hydroxy-l,4-naphthoquinone). Based on the $LD_{50}$ values of juglone and its derivatives, the most toxic compound against D. farinae was juglone ($0.076{\mu}g/cm^3$), followed by benzyl benzoate ($9.143{\mu}g/cm^3$) and 2methyl-l,4-naphthoquinone ($40.0{\mu}g/cm^3$). These results indicate that the acaricidal activity of C. sappan heartwoods is likely to be the result of the effects of juglone. Additionally, juglone treatment was shown to effect a change in the color of the cuticles of house dust mites, from colorless-transparent to dark brownish-black. Accordingly, as a naturally occurring acaricidal agent, C. sappan heartwood-derived juglone should prove to be quite 'useful as a potential control agent, lead compound, and house dust mite indicator.

• Parasites, Hosts and Diseases
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• 제46권3호
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• pp.187-189
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• 2008

House dust mites produce inhalant allergens of importance to allergic patients, We measured the major group 1 allergens, Der p 1 and Der f 1, from the house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farina, respectively in 100 randomly selected domestic homes from Cheonan, Korea, Dust samples were collected by vacuuming from the living room floor and 1 mattress in each home, Der p 1 and Der f 1 were measured by double mono-clonal ELISA. Der p 1 levels were very low, with geometric mean levels for floors and mattresses being $0.11\;{\mu}g/g$ (range: 0.01-4.05) and $0.14\;{\mu}g/g$ (range: 0.01-30.0), respectively. Corresponding levels of Der f 1 were higher, $7.46\;{\mu}g/g$ (range: 0.01-262.9) and $10.2\;{\mu}g/g$ (range: 0.01-230.9) for floors and mattresses, respectively. D. farinae appears to be the dominant house dust mite in Cheonan.

• 한국동물위생학회지
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• 제33권2호
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• pp.167-171
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• 2010

Majority of asthma and atopic dermatitis are known to be spontaneously sensitive to house dust mite allergen. Control of house dust mite populations has been principally achieved by using chemical insecticides. But the risk to human health would be a potential problem. Moreover, house dust mite remain as allergens even after death. So, It is more effective method keeping the house dust mites away than killing them. The use of plant-derived repellents has been considered as a promising alternative to chemical repellents. Eucalyptus is a diverse genus of flowering trees in the myrtle family, Myrtaceae. It has insect repellent properties and is an active ingredient in some commercial mosquito repellents. These studies were carried out to investigate repellent effects of Eucalyptus oil against house dust mites and compare lemongrass oil. Eucalyptus oil and lemongrass oil were exposed at different doses (0.2, 0.1, 0.05, 0.025, 0.0125, $0.00625{\mu}l/cm^2$) and different times (1, 3, 6 hours) on house dust mites. The most effective dose of Eucalyptus and lemongrass oils against house dust mites was $0.1{\mu}l/cm^2$ and $0.025{\mu}l/cm^2$. Each repellent effect(%) in most effective dose was 90.3% (Eucalyptus) and 80.8% (lemongrass).

• 한국동물위생학회지
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• 제32권1호
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• pp.87-92
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• 2009

The avoidance of the allergen of the house dust mite is one of the challenges to reduce and treat the symptoms of allergic diseases. Accordingly, various acaricidal agents are being used to kill the mite, but just killing it leaves the remains of it, which still act as the allergen. Therefore expelling the mite is thought as best policy to avoid the mite allergen. For this, some materials have been applied to repellent agents against the mite. Among them, a material with natural origin, known as the phytoncide, is being used for its repellent activity, as well as for its benefits for health. In this experiment, essential oils extracted from Korean white pine (Pinus koreaiensis S. et Z.) and hinoki cypress (Chamaecyparis obtusa), which are widely used as the source of phytoncide products, are studied for demonstration of the repellent effect against the house dust mites, Dermatophagoides farinae and D. pteronyssinus. Direct contact method was used to evaluate the repellent effect (%). And the results suggest the oils have a significant effect enough to be used as a source of repellent agent. For the repellent effect, the most effective concentration was $0.5{\mu}l/cm^2$ at 45 minutes both in the white pine and the hinoki oil.

• Parasites, Hosts and Diseases
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• 제44권2호
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• pp.133-138
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• 2006

This experiment was undertaken to screen the acaricidal effects of herb essential oils (pennyroyal, ylang ylang, citronella, lemon grass, tea tree, and rosemary) at different doses (0.1, 0.05, 0.025, 0.0125, and $0.00625{\mu}l/cm2$) and exposure times (5, 10, 20, 20, 30 and 60 min) on house dust mites Dermatophgoides farinae and D. pteronyssinus. The most effective acaricidal components of pennyroyal (Mentha pulegium) were analyzed using a gas chromatography-mass spectrometer (GC-MS). Of these essential oils, the most effective was pennyroyal, which is composed essentially of pulegone (> 99%), at a dose of $0.025{\mu}l/cm^2$, which at an exposure time of 5 min killed more than 98% of house dust mites. In the pennyroyal fumigation test, the closed method was more effective than the open method and maximum acaricidal effect was 100% at $0.025{\mu}l/cm^2$, 60 min. The results show that herb essential oils, in particular, pennyroyal was proved to have potent acaricidal activity.

• 대한의생명과학회지
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• 제18권4호
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• pp.435-437
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• 2012

House dust mite (HDM) is important in the pathogenesis of allergic diseases including asthma and atopic dermatitis. Dermatophagoides pteronissinus (Dp) is one of major HDM allergens. In this study, we investigated that Dp extract (DpE) affects on the chemotactic activity of monocytes isolated from the peripheral blood. DpE inhibited the migration of human monocytes in response to CC chemokines such as MIP-$1{\alpha}$, RANTES, HCC-4, MCP-1, and TARC. DpE did not alter the expression of CC chemokine receptors (CCRs) such as CCR1, CCR2, CCR3, CCR4, and CCR5. These results indicate that DpE blocks the chemotaxis of human monocytes and its mechanism is not involved in alteration of CCR expression. Better understanding of the effect of DpE on monocytes will enable elucidation of the role of Dp in the development of allergic diseases.

• 한국임상수의학회지
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• 제19권2호
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• pp.215-218
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• 2002

It is known that house dust mites which settle on sofa, carpet, and dust in the house ignite asthma and allergic rhinitis. Dermatophagoides farinae and Dermatophagoides pteronyssinus distribute widely and densely in Korea. In this study we carried out to identify that shed hair of dogs which was applied topically with fipronil ($\Frontline^{circledR}$) kill house dust mites. We co-cultured house dust mites with fipronil-applied hair of dog during 12 hours, and then compared the death rate of mites here with that cultured without hair. The larger amount of hair, the more mites were killed. The death rate of mites was higher than control significantly until 2 weeks after $\Frontline^{circledR}$ application. Hair which had been shed at 3 days after $\Frontline^{circledR}$ application always killed the smaller number of mites than not shed. The present study suggested that the use of $\Frontline^{circledR}$ to our pets control house dust mites effectively.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2001년도 합동 춘계 학술발표회
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• pp.74-74
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• 2001

• KSBB Journal
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• 제22권1호
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• pp.58-61
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• 2007

본 연구에서는 은행잎에 존재하는 생리활성 물질이 추출용매와 추출조건에 따른 D. pteronyssinus에 대한 살충 실험을 통해 최적 추출 조건을 확립하였다. 은행잎 성분 중 bilobalide가 D. pteronyssinus에 살충효과가 있음을 검증하였으며, 다양한 은행잎 추출조건에 따른 살충실험을 한 결과 48시간 이후 약 68%$\sim$80%의 치사율을 보였다. 종합적으로 판단해 볼 때 경제성과 치사율을 고려하면 80$^{\circ}C$에서 온수추출물 (GLW80)이 가장 최적추출 조건이다.