• Title/Summary/Keyword: Department of Health Science

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Comparison of Four Commercial ELISA Kits and In-House Immunoblotting for Diagnosis of Helicobacter pylori Infection

  • Jeong, Hoar Lim;Jung, Yang-Sook;Jun, Jin-Su;Yeom, Jung Sook;Park, Ji Sook;Seo, Ji-Hyun;Lim, Jae-Young;Park, Chan-Hoo;Woo, Hyang-Ok;Youn, Hee-Shang;Ko, Gyung-Hyuck;Baik, Seung-Chul;Lee, Woo-Kon;Cho, Myung-Je;Rhee, Kwang-Ho
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.15 no.2
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    • pp.85-90
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    • 2012
  • Purpose: Commercial enzyme-linked immunosorbent assay (ELISA) kits have been considered less reliable for children than for adults. The aim of this study was to compare four ELISA kits and in-house immunoblotting based on the analysis of anti-H. pylori-IgG antibody reactivity. Methods: A total of 399 serum samples were collected at the GNU Hospital during 1998-1999. All sera were tested using ELISA and immunoblotting. Statistically significant differences were determined by the $x^2$ test. Results: The overall seropositivity rates using GAP IgG, Genedia IgG, HM-CAP, Pyloriset EIA-G, and immunoblotting were 13.0%, 25.1%, 18.3%, 15.8%, and 62.9%, respectively. Immunoblotting showed a higher seropositivity rate than did all four ELISA kits in all age groups. Genedia IgG had the highest seropositivity among the ELISA kits. The seropositivity rate for children aged 13 to 18 months was lowest, and that of children aged 15 years was highest (90.0%). The seropositivity rate for children aged 7 months to 5 years was significantly lower than that for children aged 6 to 15 years among the four ELISA kits (p<0.0001) and immunoblotting (p=0.02). Conclusion: Immunoblotting is the most sensitive test for detection of anti-Helicobacter pylori IgG antibodies among the serological tests in this study. These results emphasize the need for standardization when commercial ELISA tests are used in different nations or in young age groups. Immunoblotting could be a suitable noninvasive assay for serodiagnosis and seroepidemiologic study of H. pylori infection in Korean children.

Characterization of airborne bioaerosol concentration at the apartment in chungnam area

  • Son, Bu-Soon;Park, Jong-An;Song, Mi-Ra;Yang, Won-Ho;Jung, Hong-Ryang
    • Proceedings of the Korean Environmental Health Society Conference
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    • 2005.12a
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    • pp.83-84
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    • 2005
  • This research was performed to measure the concentration distribution of Bioaero sol in apartment houses in the region of Chungnam (Chunan, Asan) for 1 month, December, 2004. The results are as follows.

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The Impact of the Financial Crisis on Lifestyle Health Determinants Among Older Adults Living in the Mediterranean Region: The Multinational MEDIS Study (2005-2015)

  • Foscolou, Alexandra;Tyrovolas, Stefanos;Soulis, George;Mariolis, Anargiros;Piscopo, Suzanne;Valacchi, Giuseppe;Anastasiou, Foteini;Lionis, Christos;Zeimbekis, Akis;Tur, Josep-Antoni;Bountziouka, Vassiliki;Tyrovola, Dimitra;Gotsis, Efthimios;Metallinos, George;Matalas, Antonia-Leda;Polychronopoulos, Evangelos;Sidossis, Labros;Panagiotakos, Demosthenes B.
    • Journal of Preventive Medicine and Public Health
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    • v.50 no.1
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    • pp.1-9
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    • 2017
  • Objectives: By the end of the 2000s, the economic situation in many European countries started to deteriorate, generating financial uncertainty, social insecurity and worse health status. The aim of the present study was to investigate how the recent financial crisis has affected the lifestyle health determinants and behaviours of older adults living in the Mediterranean islands. Methods: From 2005 to 2015, a population-based, multi-stage convenience sampling method was used to voluntarily enrol 2749 older adults (50% men) from 20 Mediterranean islands and the rural area of the Mani peninsula. Lifestyle status was evaluated as the cumulative score of four components (range, 0 to 6), that is, smoking habits, diet quality (MedDietScore), depression status (Geriatric Depression Scale) and physical activity. Results: Older Mediterranean people enrolled in the study from 2009 onwards showed social isolation and increased smoking, were more prone to depressive symptoms, and adopted less healthy dietary habits, as compared to their counterparts participating earlier in the study (p<0.05), irrespective of age, gender, several clinical characteristics, or socioeconomic status of the participants (an almost 50% adjusted increase in the lifestyle score from before 2009 to after 2009, p<0.001). Conclusions: A shift towards less healthy behaviours was noticeable after the economic crisis had commenced. Public health interventions should focus on older adults, particularly of lower socioeconomic levels, in order to effectively reduce the burden of cardiometabolic disease at the population level.

Susceptibility of several strains of mice to Echinostoma hortense infection

  • Lee, Kyu-Jae;Park, Seung-Kyu;Im, Jee-Aee;Kim, Soo-Kie;Kim, Geun-Ha;Kim, Gwang-Young;Yang, Eun-Ju;Ryang, Yong-Suk
    • Parasites, Hosts and Diseases
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    • v.42 no.2
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    • pp.51-56
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    • 2004
  • Susceptibilities of 5 different mice strains, including C3H/HeN, BALB/c, C57BL6, FvB and ICR, to Echinostoma hortense infection, was evaluated. The worm expulsion rate, worm size and egg production were observed from 1 to 8 weeks after infection with 30 metacercariae. C3H/HeN and ICR mice showed the highest worm maturation rates. The worm recovery rate and the number of eggs per gram (EPG) of feces was also higher in C3H/HeN and ICR mice than in BALB/c, C57BL6, and FvB mice. It is suggested that E. hortense is highly infectious to ICR and C3H/HeN mice, but not to the other strains of mice. Based on the results obtained, we believe that the susceptibility of different mouse strains to E. hortense infection is dependent on the genetic and immunologic back-ground of mice.

The Determination of Ethyl Isocyanate and Propyl Isocyanate by GC/FID (에틸 이소시아네이트와 프로필 이소시아네이트의 GC/FID 분석)

  • Lee, Eun-Jung;Yoo, Chul;Choi, Hong-Soon;Park, Jun-Ho;Yoon, Ju-Song;Cho, Young-Bong
    • Environmental Analysis Health and Toxicology
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    • v.15 no.1_2
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    • pp.53-59
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    • 2000
  • The purpose of this study is the development of the simple and precise determination method of ethyl isocyanate (EIC) and propyl isocyanate (PIC) through derivatization using secondary aliphatic amines by gas chromatography with flame ionization detector. The urea derivatives are quantitatively and simultaneously derived from EIC and PIC with secondary aliphatic amines such as dipropylamine, dibutylamine. and dipentylamine in methylene chloride, and confirmed by thin layer chromatography and gas chromatography with mass selective detector. For GC/FID, according to the increasing carbon atom of the amines, the retention time and peak area of the urea derivatives are increased. The instrumental detection limits for EIC and PIC were about 23.3∼34.8 $\mu\textrm{g}$ and 21.6∼28.9 $\mu\textrm{g}$, respectively.

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