• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2007년도 추계학술대회 논문집
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• pp.514-514
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• 2007

1차원 구조를 갖는 나노 와이어들은 나노 소자를 구현하기 위한 building-block으로 많은 과학자들의 주목을 받고 있고 또한 연구되고 있다. 하지만 그것을 정확하게 위치시키고 일정한 간격으로 정렬시키기 위한 기술 개발은 아직도 해결해야 할 큰 과제로 남아 있다. 이 논문에서, 우리는 ahsing 기술과 표면 패터닝 기술을 이용하여 대면적의 실리콘웨이퍼 위에 DNA(deoxyribonucleic acid)를 기반으로 한 금 나노 와이어를 정확하게 위치시키고 일정한 간격으로 정렬시킬 수 있는 새로운 제어 기술을 제안한다. 먼저 우리는 포토 리소그래피 공정과 $O_2$ 플라즈마 ashing 기술을 이용하여 선폭을 100 nm로 감소 시켰다. 그리고 자기조립단분자막 (self-assembled monolayers; SAMs) 방법과 lift-off 공정을 반복함으로서 1-octadecyltrichlorosilane(OTS) 층과 aminopropylethoxysilane(APS) 층을 형성하였다. 마지막으로 DNA 용액을 샘플 표면 위에 도포하고 분자 빗질 방법으로 DNA를 한 방향으로 정렬 시켰고 금 나노입자 용액을 처리하였다. 그 결과 금 나노 와이어는 $10{\mu}m$ 간격으로 일정하게 정열 되었고, APS 층에만 정확하게 정렬되었다. 우리는 금 나노 와이어를 관찰하기 위하여 원자간력 현미경 (Atomic Force Microscope AFM)을 사용하였다.

• 대한한방부인과학회지
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• 제25권4호
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• pp.154-160
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• 2012

Objectives: This report is aimed to investigate the efficacy of Korean medicine including intravenous Cultivated Wild Ginseng(Panax ginseng C.A. Meyer) Pharmacopuncture(CWGP) with intractable cervical dysplasia patient. Methods: A 49-year-old woman who was diagnosed as cervical dysplasia on Feb 2012 was treated with Korean medicine for 3 months. Korean medicine includes intravenous CWGP, acupuncture, moxibustion and herbal medicine. The effect of therapies was evaluated with human papillomavirus deoxyribonucleic acid(HPV DNA) testing. And lower limb cold sense and genital pruritus were recorded with Visual Analogue Scale(VAS). Results: Lower limb cold sense and genital pruritus were decreased from VAS 8-9(2012.04.11.) to VAS 1-2(2012.07.10.). HPV DNA test result was changed from positive to negative. From these results, this report suggests that the Korean medicine, especially CWGP may be a useful method to treat intractable cervical dysplasia. Conclusions: This report shows that Korean medical treatments are effective on intractable cervical dysplasia.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권7호
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• pp.3794-3820
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• 2019

In recent years, a cloud environment with the ability to detect illegal behaviours along with a secured data storage capability is much needed. This study presents a cloud storage framework, wherein a 128-bit encryption key has been generated by combining deoxyribonucleic acid (DNA) cryptography and the Hill Cipher algorithm to make the framework unbreakable and ensure a better and secured distributed cloud storage environment. Moreover, the study proposes a DNA-based encryption technique, followed by a 256-bit secure socket layer (SSL) to secure data storage. The 256-bit SSL provides secured connections during data transmission. The data herein are classified based on different qualitative security parameters obtained using a specialized fuzzy-based classification technique. The model also has an additional advantage of being able to decide on selecting suitable storage servers from an existing pool of storage servers. A fuzzy-based technique for order of preference by similarity to ideal solution (TOPSIS) multi-criteria decision-making (MCDM) model has been employed for this, which can decide on the set of suitable storage servers on which the data must be stored and results in a reduction in execution time by keeping up the level of security to an improved grade.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제24권1호
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• pp.30-37
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• 2021

Purpose: To investigate the differences in the colon microbiota composition of Hirschsprung's disease (HSCR) patients with and without a history of postoperative Hirschsprung's associated enterocolitis (HAEC). Methods: Colon tissue microbiota was characterized by bacterial deoxyribonucleic acid (DNA) extraction and 16S rDNA sequencing for taxonomic classification and comparison. Results: The sequence diversity richness within samples was significantly higher in samples from patients with a history of postoperative HAEC. We observed an increased relative abundance of the phyla Bacteroidetes, Firmicutes and Cyanobacteria in HAEC patients and Fusobacteria, Actinobacteria and Proteobacteria in HSCR patients and, an increased relative abundance of the genera Dolosigranulum, Roseouria and Streptococcus in HAEC patients and Propionibacterium and Delftia in HSCR patients. Conclusion: Our findings provide evidence that the colon tissue microbiota composition is different in HSCR patients with and without postoperative HAEC.

• Journal of Information Processing Systems
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• 제18권1호
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• pp.59-74
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• 2022

During the last decade, the security of digital images has received considerable attention in various multimedia transmission schemes. However, many current cryptosystems tend to adopt a single-layer permutation or diffusion algorithm, resulting in inadequate security. A hierarchical bilateral diffusion architecture for color image encryption is proposed in response to this issue, based on a hyperchaotic system and DNA sequence operation. Primarily, two hyperchaotic systems are adopted and combined with cipher matrixes generation algorithm to overcome exhaustive attacks. Further, the proposed architecture involves designing pixelpermutation, pixel-diffusion, and DNA (deoxyribonucleic acid) based block-diffusion algorithm, considering system security and transmission efficiency. The pixel-permutation aims to reduce the correlation of adjacent pixels and provide excellent initial conditions for subsequent diffusion procedures, while the diffusion architecture confuses the image matrix in a bilateral direction with ultra-low power consumption. The proposed system achieves preferable number of pixel change rate (NPCR) and unified average changing intensity (UACI) of 99.61% and 33.46%, and a lower encryption time of 3.30 seconds, which performs better than some current image encryption algorithms. The simulated results and security analysis demonstrate that the proposed mechanism can resist various potential attacks with comparatively low computational time consumption.

• Animal Bioscience
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• 제36권3호
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• pp.385-403
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• 2023

Male fertility is affected by multiple endogenous stressors, including reactive oxygen species (ROS), which greatly deteriorate the fertility. However, physiological levels of ROS are required by sperm for the proper accomplishment of different cellular functions including proliferation, maturation, capacitation, acrosomal reaction, and fertilization. Excessive ROS production creates an imbalance between ROS production and neutralization resulting in oxidative stress (OS). OS causes male infertility by impairing sperm functions including reduced motility, deoxyribonucleic acid damage, morphological defects, and enhanced apoptosis. Several in-vivo and in-vitro studies have reported improvement in quality-related parameters of sperm following the use of different natural and synthetic antioxidants. In this review, we focus on the causes of OS, ROS production sources, mechanisms responsible for sperm damage, and the role of antioxidants in preserving sperm fertility.

• Asian-Australasian Journal of Animal Sciences
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• 제26권1호
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• pp.72-81
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• 2013

Four Luxi beef cattle ($400{\pm}10$ kg) fitted with ruminal, duodenal and ileal cannulas were used in a $4{\times}4$ Latin square to assess the effects of soybean small peptide (SSP) infusion on rumen fermentation, diet digestion and flow of nutrient in the gastrointestinal tract. The ruminal infusion of SSP was 0 (control), 100, 200 and 300 g/d. Ruminal SSP infusion linearly (p<0.01) and quadratically (p<0.01) increased microbial protein synthesis and rumen ammonia-N concentration. Concentrations of total volatile fatty acid were linearly increased (p = 0.029) by infusion SSP. Rumen samples were obtained for analysis of microbial ecology by real-time PCR. Populations of rumen Butyrivibrio fibrisolvens, Streptococcus bovis, Ciliate protozoa, Ruminococcus flavefaciens, and Prevotella ruminicola were expressed as a proportion of total Rumen bacterial 16S ribosomal deoxyribonucleic acid (rDNA). Butyrivibrio fibrisolvens populations which related to total bacterial 16S rDNA were increased (p<0.05), while Streptococcus bovis populations were linearly (p = 0.049) and quadratically (p = 0.020) decreased by infusion of SSP. Apparent rumen digestibility of DM and NDF were (Q, p<0.05; L, p<0.05) increased with infusion SSP. Total tract digestion of DM, OM and NDF were linearly (p<0.01) and quadratically (p<0.01) increased by infusing SSP. The flow of total amino acids (AA), essential amino acids (EAA) and individual amino acids were linearly (p<0.01) and quadratically (p<0.01) increased with infusion SSP. The digestibility of Lysine was quadratically (p = 0.033) increased and apparent degradability of Arginine was linearly (p = 0.032) and quadratically (p = 0.042) increased with infusion SSP. The results indicated that infusion SSP could improve nutrient digestion, ruminal fermentation and AA availability.

• 대한약침학회지
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• 제18권3호
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• pp.32-41
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• 2015

Objectives: Condurango (Gonolobus condurango) extract is used by complementary and alternative medicine (CAM) practitioners as a traditional medicine, including homeopathy, mainly for the treatment of syphilis. Condurango bark extract is also known to reduce tumor volume, but the underlying molecular mechanisms still remain unclear. Methods: Using a cervical cancer cell line (HeLa) as our model, the molecular events behind condurango extract's (CE's) anticancer effect were investigated by using flow cytometry, immunoblotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Other included cell types were prostate cancer cells (PC3), transformed liver cells (WRL-68), and peripheral blood mononuclear cells (PBMCs). Results: Condurango extract (CE) was found to be cytotoxic against target cells, and this was significantly deactivated in the presence of N-acetyl cysteine (NAC), a scavenger of reactive oxygen species (ROS), suggesting that its action could be mediated through ROS generation. CE caused an increase in the HeLa cell population containing deoxyribonucleic acid (DNA) damage at the G zero/Growth 1 (G0/G1) stage. Further, CE increased the tumor necrosis factor alpha ($TNF-{\alpha}$) and the fas receptor (FasR) levels both at the ribonucleic acid (RNA) and the protein levels, indicating that CE might have a cytotoxic mechanism of action. CE also triggered a sharp decrease in the expression of nuclear factor kappa-light-chain-enhancer of activated B cells ($NF-{\kappa}B$) both at the RNA and the protein levels, a possible route to attenuation of B-cell lymphoma 2 (Bcl-2), and caused an opening of the mitochondrial membrane's permeability transition (MPT) pores, thus enhancing caspase activities. Conclusion: Overall, our results suggest possible pathways for CE mediated cytotoxicity in model cancer cells.

• Journal of Microbiology and Biotechnology
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• 제17권5호
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• pp.812-821
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• 2007

The ecosystems of certain abandoned mines contain arsenic-resistant bacteria capable of performing detoxification when an ars gene is present in the bacterial genome. The ars gene has already been isolated from Pseudomonas putida and identified as a member of the membrane transport regulatory deoxyribonucleic acid family. The arsenite-oxidizing bacterial strains isolated in the present study were found to grow in the presence of 66.7 mM sodium arsenate($V;\;Na_2HAsO_4{\cdot}7H_2O$), yet experienced inhibited growth when the sodium arsenite($III;\;NaAsO_2$) concentration was higher than 26 mM. Batch experiment results showed that Pseudomonas putida strain OS-5 completely oxidized 1 mM of As(III) to As(V) within 35 h. An arsB gene encoding a membrane transport regulatory protein was observed in arsenite-oxidizing Pseudomonas putida strain OS-5, whereas arsB, arsH, and arrA were detected in strain OS-19, arsD and arsB were isolated from strain RW-18, and arsR, arsD, and arsB were found in E. coli strain OS-80. The leader gene of arsR, -arsD, was observed in a weak acid position. Thus, for bacteria exposed to weak acidity, the ars system may cause changes to the ecosystems of As-contaminated mines. Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines.

• 대한약침학회지
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• 제19권1호
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• pp.37-44
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• 2016

Objectives: This study examined the relative efficacies of a derivative of betulinic acid (dBA) and its poly (lactide-co-glycolide) (PLGA) nano-encapsulated form in A549 lung cancer cells in vivo and in co-mutagen [sodium arsenite (SA) + benzo[a]pyrene (BaP)]-induced lung cancer in mice in vivo. Methods: dBA was loaded with PLGA nanoparticles by using the standard solvent displacement method. The sizes and morphologies of nano-dBA (NdBA) were determined by using transmission electron microscopy (TEM), and their intracellular localization was verified by using confocal microscopy. The binding and interaction of NdBA with calf thymus deoxyribonucleic acid (CT-DNA) as a target were analyzed by using conventional circular dichroism (CD) and melting temperature (Tm) profile data. Apoptotic signalling cascades in vitro and in vivo were studied by using an enzyme-linked immunosorbent assay (ELISA); the ability of NdBA to cross the blood-brain barrier (BBB) was also examined. The stage of cell cycle arrest was confirmed by using a fluorescence-activated cell-sorting (FACS) data analysis. Results: The average size of the nanoparticles was ~ 110 nm. Confocal microscopy images confirmed the presence of NdBA in the cellular cytoplasm. The bio-physical properties of dBA and NdBA ascertained from the CD and the Tm profiles revealed that NdBA had greater interaction with the target DNA than dBA did. Both dBA and NdBA arrested cell proliferation at G0/G1, NdBA showing the greater effect. NdBA also induced a greater degree of cytotoxicity in A549 cells, but it had an insignificant cytotoxic effect in normal L6 cells. The results of flow cytometric, cytogenetial and histopathological studies in mice revealed that NdBA caused less nuclear condensation and DNA damage than dBA did. TEM images showed the presence of NdBA in brain samples of NdBA fed mice, indicating its ability to cross the BBB. Conclusion: Thus, compared to dBA, NdBA appears to have greater chemoprotective potential against lung cancer.