• Journal of Dental Rehabilitation and Applied Science
• /
• v.24 no.1
• /
• pp.105-112
• /
• 2008

The purpose of this study was to evaluate the contact angle made by 3 kinds of self etching primers (Clearfil SE Bond, AdheSE, and Tyrian) on dentin and to measure the microtensile bond strength of resin composite to dentin using these self-etching primers. Contact angle between each of 3 self etching primers and polished dentin surface was measured (n=30) by contact angle analyzer and the result was analyzed by One-way ANOVA. For the measurement of microtensile bond strength, polished dentin surface was treated with each of 3 self etching primers and dentin adhesives. Z-250 composite resin was built-up with a height of 5 mm on the adhesive-treated surface and light cured for 40s with a halogen light curing unit. Thereafter, each tooth was sectioned into slabs perpendicular to the bonded interface and trimmed (n=45). The microtensile bond strength was measured with universal testing machine and the result was analyzed with Kruskal-Wallis test. AdheSE group showed the highest contact angle followed by Clearfil SE group and Tyrian group (p<0.05). AdheSE group and Clearfil SE group showed significantly higher microtensile bond strength than Tyrian group (P<0.05).

• Journal of the korean academy of Pediatric Dentistry
• /
• v.27 no.3
• /
• pp.444-456
• /
• 2000

To test the shear bond strength of a new "one-bottle adhesive" system to primary dentin two commercially available one-bottle adhesives (Prime & Bond NT, Single bond) and conventional three step system(Scotchbond Multi-Purpose Plus) were included for comparison. And We observe the interfacial morphology by scanning electron microscope. 90 primary molar teeth were embedded in acrylic and buccal and lingual surface were polished to 320 grit to create standardized dentin surface for testing. After bonding of composite resin to sample surfaces according to the manufacturer s direction and 1000 times thermocycling in dwell time 30 second, Shear bond strengths of adhesives to dentin were determined using universal testing machine and analyzed by ANOVA test. Another groups of specimens were treated by hydrochloric acid to secure the resin only and those tags were evaluated under SEM for their length and forms and the morphology of the bonding sites were also observed. The result are as follows. 1. Group I(Prime & Bond NT) showed higher shear bond strength than group iI(Single Bond) and III(Scotchbond Multi Purpose Plus) but no statistically significant difference was founded between groups(p>.05). 2. Relating long resin tags of $70-120{\mu}m$ were observed in samples of all groups under SEM. We could observed hybrid layer, resin tag and many lateral branches in every group. But, we observed in group III rare lateral branched than other two group and discontinuous hybrid layer.

• Restorative Dentistry and Endodontics
• /
• v.27 no.4
• /
• pp.382-388
• /
• 2002

Compomer is composed of matrix and filler : matrix is made of the combination of resins and polycarboxylic molecules that are light-cured, and a filler is a glass component which is capable of ion-release. The resin content of compomers produces polymerization shrinkage which can adversely affect marginal adaptation. Pretreatment is a fundamental step which is treated with conditioner or primer in the use of these materials. Microleakage of restorative materials has been investigated mostly by dye penetration method. Dye penetration method was not quantitative and not measured repeatedly. Fluid filtration method, introduced and developed by Pashley's group, has been extensively used for 20 years for research purpose to understand the physiology of dentin, as well as the effects of various restorative treatments on dentin permeability. It permits quantitative, nondestructive measurment of microleakage in a longitudinal manner. The purpose of this study was to evaluate the change of dentin permeability according to the process of compomer restoration. In this study. Cl V cavities were prepared on buccal surface of thirty extracted human molars. The prepared cavities were etched by 37% phosphoric acid. The experimental teeth were randomly divided into three groups. Each group was treated with following materials Group 1 : Prime & Bond NT/Dyract AP, Group2： Single Bond/F2000 compomer, Group 3 : Syntac Single Component/Compoglass. The bonding agent and compomer were applied for each group following manufacturers information. Dentin permeability of each group was measured at each process by fluid filtration method; Step 1 : preparation(smear layer). Step 2 : etching(smear layer removal), Step 3 : applying the bonding agent, Step 4 : filling the compomer. Dentin permeability was expressed by hydraulic conductance ($\mu\textrm{l}$ min$^{-1}$cm$H_2O$$^{-1}$). The data were analysed statistically using One-way ANOVA and Sheffe's method. The results were as follows : 1. Dentin permeability differences between each process were significant except between step 1 and step 2(p<0.01). 2. Dentin permeability after removal of smear layer was highly increased(p<0.01). 3. In most case, decrease of dentin permeability was obtained by applying bonding agent(p<0.01). 4. Dentin permeability differences among the experimental groups were not significant(p>0.05). 5. None of compomers used in this study showed perfect seal at the interface.

• The Journal of Advanced Prosthodontics
• /
• v.5 no.3
• /
• pp.226-233
• /
• 2013

PURPOSE. This study evaluated the adhesion of 10-MDP containing self-etch and self-adhesive resin cements to dentin with and without the use of etch-and-rinse technique. MATERIALS AND METHODS. Human third molars (N=180) were randomly divided into 6 groups (n=30 per group). Conventional (Panavia F2.0, Kuraray-PAN) and self-adhesive resin cements (Clearfil SA, Kuraray-CSA) were bonded to dentin surfaces either after application of 3-step etch-and-rinse (35% $H_3PO_4$ + ED Primer) or two-step self-etch adhesive resin (Clearfil SE Bond). Specimens were subjected to shear bond strength test using the universal testing machine (0.5 mm/min). The failure types were analyzed using a stereomicroscope and quality of hybrid layer was observed under a scanning electron microscope. The data (MPa) were analyzed using two-way ANOVA and Tukey's tests (${\alpha}$=.05). RESULTS. Overall, PAN adhesive cement showed significantly higher mean bond strength ($12.5{\pm}2.3-14.1{\pm}2.4$ MPa) than CSA cement ($9.3{\pm}1.4-13.9{\pm}1.9$ MPa) (P<.001). Adhesive failures were more frequent in CSA cement groups when used in conjunction with two-step self-adhesive (68%) or no adhesive at all (66%). Hybrid layer quality was inferior in CSA compared to PAN cement in all conditions. CONCLUSION. In clinical situations where bonding to dentin substrate is crucial, both conventional and self-adhesive resin cements based on 10-MDP can benefit from etch-and-rinse technique to achieve better quality of adhesion in the early clinical period.

• Restorative Dentistry and Endodontics
• /
• v.23 no.1
• /
• pp.487-501
• /
• 1998

In the case of CAD/CAM ceramic inlay restorations, if isthmus width is widened too much, it may cause fracture of remaining tooth structure or loss of bonding at the luting interface because of excessive displacement of buccal or lingual cusps under occlusal loads. So to clarify the criterior of widening isthmus width, this study was designed to test the tensile bond strength and bond failure mode between dentin and ceramic cemented with luting composite resin cements. Cylindrical ceramic blocks(Vita Cerec Mark II, d=4mm) were bonded to buccal dentin of 40 freshly extracted third molars with 4 luting composite resin cements(group1 : Scotchbond Resin Cement/Scotchbond Multi-Purpose, group2 : Duolink Resin Cement/ All-Bond 2, group3: Bistite Resin Cement/Ceramics Primer, and group4:Superbond C&B). Tensile bond test was done under universal testing machine using bonding and measuring alignment blocks(${\phi}ilo$ & Urn, 1992). After immersion of fractured samples into 1 % methylene blue for 24 hours, failure mode was analysed under stereomicroscope and SEM. Results: The tensile bond strength of goup 1, 2 & 4 was $13.97{\pm}2.90$ MPa, $16.49{\pm}3.90$ MPa and $16.l7{\pm}4.32$ MPa, respectively. There was no statistical differences(p>0.05). But, group 3 showed significantly lower bond stregnth($5.98{\pm}1.l7$ MPa, p<0.05). In almost all samples, adhesive fractures between dentin and resin cements were observed. But, in group 1, 2 & 4, as bond strength increased, cohesive fracture within resin cement was observed simultaneously. And, in group 3, as bond strength decreased, cohesive fracture between hybrid layer and composite resin cement was also observed. Cohesive fracture within dentin and porcelain adhesive fracture were not observed. In conclusion, although adhesive cements were used in CAD/CAM -fabricated ceramic inlay restorations, the conservative priciples of cavity preparation must be obligated.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.33 no.3
• /
• pp.377-387
• /
• 2006

The objective of this study was to evaluate the influence of multiple adhesive coatings on the thickness of hybrid and adhesive layer and shear bond strength(SBS) of self-etch adhesives and self-etch primer adhesives. The buccal or lingual crown dentin of extracted human molars was used. Self-etch adhesives or self-etch primer adhesives were applied 1, 2 and 3 times on the dentin before light curing. In another group adhesives were reapplied after light curing first layer. Treated surfaces were prepared to measure the thickness of hybrid and adhesive layer with SEM, and shear bond strength to dentin using an Instron machine. The following results were obtained : 1. The adhesive layers increased with the number of coatings(p<0.05) with all adhesives. Adpor Prompt L-Pop and Xeno III were significantly thinner than self-etch primer adhesives (p<0.05). 2. The thickness of hybrid layers increased with the number of coatings (p<0.05). 3. The shear bonding strength of Unifil Bond and Clearfill SE Bond were higher than Scotchbond Multipurpose Plus and Adpor Prompt L-Pop (p<0.05), and similar with Xeno III. 4. The shear bond strength increased significantly with the number of coatings in Adpor Prompt L-Pop(p<0.05), but decreased at 3 times in AdheSE Bond(p>0.05). 5. In Adpor Prompt L-Pop and Xeno III, the shear bond strength decreased when adhesives were reapplied after curing the first adhesive layer.

• Restorative Dentistry and Endodontics
• /
• v.28 no.1
• /
• pp.23-33
• /
• 2003

The purpose of this study was to compare in vitro interfacial relationship of restorations bonded with three self-etching primer adhesives and one self-etching adhesive. Class I cavity preparations were prepared on twenty extracted human molars. Prepared teeth were divided into four groups and restored with four adhesives and composites Clearfil SE $Bond/Clearfil^{TM}$ AP-X (SE), UniFil $Bond/UniFil^{\circledR}$ F (UF), FL $Bond/Filtek^{TM}$ Z 250 (FL) and Prompt $L-Pop/Filtek^{TM}$ Z 250 (LP) After storing in distilled water of room temperature for 24 hours, the specimens were vertically sectioned and decalcified. Morphological patterns between the enamel/dentin and adhesives were observed under SEM. The results of this study were as follows : 1. They showed close adaptation between enamel and SE, UF and FL except for LP. 2. The hybrid layer in dentin was $2{\;}\mu\textrm{m}$ thick in SE, $1.5{\;}\mu\textrm{m}$ thick in UF, and $0.4{\;}\mu\textrm{m}$ in both FL and LP. So, the hybrid layers of SE and UF were slightly thicker than that of FL and LP. 3. The lengths and diameters of resin tags in UF and FL were similar, but those of LP were slightly shorter and slenderer than those of SE. 4. The resin tags were long rod shape in SE, and funnel shape in other groups Within the limitations of this study, it was concluded that self-etching primer adhesives showed close adaptation on enamel. In addition, the thickness of hybrid layer ranged from $0.4-1.5{\;}\mu\textrm{m}$ between adhesives and dentin. The resin tags were long rod or funnel shape, and dimension of them was similar or different among adhesives.

• The Journal of the Korean dental association
• /
• v.53 no.3
• /
• pp.187-194
• /
• 2015

This paper reflects on the state of the art of two kinds of tooth hard tissue (enamel and dentin) bonding with resin cement. After presenting composition of resin cement, concepts of enamel bonding and resin bonding are addressed. Special attention is devoted to the concept and advantage of self-etching technique. Finally, recommended clinical performance regarding bonding to tooth with resin cement is summarized.

• Restorative Dentistry and Endodontics
• /
• v.35 no.1
• /
• pp.13-19
• /
• 2010

The purpose of this study was to compare the microshear bond strength of a self-etching primer adhesive to dentin prepared with different diamond points, carbide burs and SiC papers, and also to determine which SiC paper yield similar strength to that of dentinal surface prepared with points or burs. Fifty-six human molar were sectioned to expose the occlusal dentinal surfaces of crowns and slabs of 1.2 mm thick were made. Dentinal surfaces were removed with three diamond points, two carbide burs, and three SiC papers. They were divided into one of eight equal groups (n = 7); Group 1: standard diamond point(TF-12), Group 2: fine diamond point (TF-12F), Group 3: extrafine diamond point (TF-12EF), Group 4: plain-cut carbide bur (no. 245), Group 5: cross-cut carbide bur (no. 557), Group 6 : P 120-grade SiC paper, Group 7: P 220-grade SiC paper, Group 8: P 800-grade SiC paper. Clearfil SE Bond was applied on dentinal surface and Clearfil AP-X was placed on dentinal surface using Tygon tubes. After the bonded specimens were subjected to uSBS testing, the mean uSBS (n = 20 for each group) was statistically compared using one-way ANOV A and Tukey HSD test. In conclusion, the use of extrafine diamond point is recommended for improved bonding of Clearfil SE Bond to dentin. Also the use of P 220-grade SiC paper in vitro will be yield the results closer to dentinal surface prepared with fine diamond point or carbide burs in vivo.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.35 no.1
• /
• pp.30-38
• /
• 2008

Dental pulp cells are assumed to possess the capacity to elaborate both bone and dentin matrix under the pathological conditions following tooth injury. The purpose of this study is to examine the effects of mineral trioxide aggregate (MTA) on various gene expression regarding dentinogenesis and cell viability assay in cultured primary human dental pulp cells. The author also examined the effects of this material on cellular alkaline phosphatase activity as a potential indicator of dentinogenesis. For gene expression on MTA, reverse transcriptase polymerase chain reaction was performed using primer sets of glyceraldehyde-3-phosphate dehydrogenase, type I collagen, alkaline phosphatase(ALP), osteonectin, and dentin sialoprotein after 2 and 4 days. Cell viability assay showed that the proportion of MTA-treated pulp cells which had been exposed for 5 days to MTA was higher than that of the control cells. Among the genes investigated in this study, ALP and osteonectin(SPARC) were increased in MTA treated group than in control. These findings suggest that this dental pulp culture system may be useful in the future as a model for studying the mechanisms underlying dentin regeneration after the treatment with MTA. Exposure to MTA material would not induce cytotoxic response in the dental pulp cells. In addition, MTA could influence the behavior of human pulp cells by increasing the ALP activity and SPARC synthesis.