• International Journal of Oral Biology
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• v.46 no.1
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• pp.15-22
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• 2021

Alpha-lipoic acid (ALA) is a naturally occurring antioxidant and has been previously used to treat diabetes and cardiovascular disease. However, the autophagy effects of ALA against oxidative stress-induced dopaminergic neuronal cell injury remain unclear. The aim of this study was to investigate the role of ALA in autophagy and apoptosis against oxidative stress in the SH-SY5Y human dopaminergic neuronal cell line. We examined SH-SY5Y phenotypes using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay (cell viability/proliferation), 4′,6-diamidino-2-phenylindole dihydrochloride nuclear staining, Live/Dead cell assay, cellular reactive oxygen species (ROS) assay, immunoblotting, and immunocytochemistry. Our data showed ALA attenuated hydrogen peroxide (H2O2)-induced ROS generation and cell death. ALA effectively suppressed Bax up-regulation and Bcl-2 and Bcl-xL down-regulation. Furthermore, ALA increased the expression of the antioxidant enzyme, heme oxygenase-1. Moreover, the expression of Beclin-1 and LC-3 autophagy biomarkers was decreased by ALA in our cell model. Combined, these data suggest ALA protects human dopaminergic neuronal cells against H2O2-induced cell injury by inhibiting autophagy and apoptosis.

• The korean journal of orthodontics
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• v.54 no.3
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• pp.171-184
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• 2024

Objective: This study aimed to determine the maxillary and mandibular basal bone regions and explore the three-dimensional positional relationship between the dentition and basal bone regions in patients with skeletal Class I and Class II malocclusions with mandibular retrusion. Methods: Eighty patients (40 each with Class I and Class II malocclusion) were enrolled. Maxillary and mandibular basal bone regions were determined using cone-beam computed tomography images. To measure the relationship between the dentition and basal bone region, the root position and root inclination were calculated using the coordinates of specific fixed points by a computer program written in Python. Results: In the Class II group, the mandibular anterior teeth inclined more labially (P < 0.05), with their apices positioned closer to the external boundary. The apex of the maxillary anterior root was positioned closer to the external boundary in both groups. Considering the molar region, the maxillary first molars tended to be more lingually inclined in females (P = 0.037), whereas the mandibular first molars were significantly more labially inclined in the Class II group (P < 0.05). Conclusions: Mandibular anterior teeth in Class II malocclusion exhibit a compensatory labial inclination trend with the crown and apex relative to the basal bone region when mandibular retrusion occurs. Moreover, as the root apices of the maxillary anterior teeth are much closer to the labial side in Class I and Class II malocclusion, the range of movement at the root apex should be limited to avoid extensive labial movement.

• Journal of Technologic Dentistry
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• v.37 no.4
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• pp.213-218
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• 2015

Purpose: The purpose of this study compared of reproducibility of prepared tooth impression scanning utilized with white and blue light scanners. Methods: To evaluate reproducibility with white and blue light scanners, the impression of premolar were rotated by $10^{\circ}{\sim}20^{\circ}$ and scanned. These data were compared with the first 3-D data (STL file), and the error sizes were measured (n=5). Independent t test was used to evaluation the reproducibility of impression of premolar with white versus blue light scanners through discrepancies of mean, RMS (${\alpha}=0.05$). Results: Discrepancies of mean with regard to reproducibility were $11.2{\mu}m$, $5.8{\mu}m$, respectively, with white and blue light scanners (p<0.047). And discrepancies of RMS with regard to reproducibility were $33.4{\mu}m$, $18.8{\mu}m$, respectively, with white and blue light scanners (p<0.045). Conclusion: Our results indicate a good reproducibility of prepared tooth impression digitized with blue light scanner more than that with white light scanner.

• The Journal of Advanced Prosthodontics
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• v.6 no.1
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• pp.22-29
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• 2014

PURPOSE. This study aimed to investigate the potential clinical application of digitized silicone rubber impressions by comparing the accuracy of zirconia 3-unit fixed partial dentures (FPDs) fabricated from 2 types of data (working model and impression) obtained from a laser scanner. MATERIALS AND METHODS. Ten working models and impressions were prepared with epoxy resin and vinyl polysiloxane, respectively. Based on the data obtained from the laser scanner (D-700; 3Shape A/S, Copenhagen, Denmark), a total of 20 zirconia frameworks were prepared using a dental CAD/CAM system (DentalDesigner; 3shape A/S, Copenhagen, Denmark / Ener-mill, Dentaim, Seoul, Korea). The silicone replicas were sectioned into four pieces to evaluate the framework fit. The replicas were imaged using a digital microscope, and the fit of the reference points (P1, P2, P3, P4, P5, P6, and P7) were measured using the program in the device. Measured discrepancies were divided into 5 categories of gaps (MG, CG, AWG, AOTG, OG). Data were analyzed with Student's t-test ($\alpha$=0.05), repeated measures ANOVA and two-way ANOVA (${\alpha}=0.05$). RESULTS. The mean gap of the zirconia framework prepared from the working models presented a narrower discrepancy than the frameworks fabricated from the impression bodies. The mean of the total gap in premolars (P=.003) and molars (P=.002) exhibited a statistical difference between two groups. CONCLUSION. The mean gap dimensions of each category showed statistically significant difference. Nonetheless, the digitized impression bodies obtained with a laser scanner were applicable to clinical settings, considering the clinically acceptable marginal fit ($120{\mu}m$).

• Journal of Technologic Dentistry
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• v.43 no.4
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• pp.168-174
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• 2021

Purpose: This study was conducted to investigate alloys spectrophotometrically including yttrium of nickel-chromium (Ni-Cr) alloys, which are used as substitutes for the regulation of beryllium and provide helpful improvements in Ni-Cr alloys. Methods: Four groups of specimens (ZN, ZY, SN and EM) were prepared for analysis. Color parameters were measured with a spectrophotometer, and color difference (∆E^*) was calculated. The t-test and one-way analysis of variance test were used to determine significant difference, and the Tukey test was used to identify where the differences were. To measure the spectroscopic reflectivity, the spectroscopic reflectance was measured and converted into CIE L^*, a^*, b^* color system. Results: The ∆E^* value of each metal ceramic group after opaque firing of Ni-Cr alloy with and without yttrium was <2, and the total group color difference (∆E^*) was below 1 in the dentin ceramic all experimental group. However, the a^* and b^* values of the metal ceramic groups were higher than that in the lithium disilicate all ceramic group, and the chroma was higher than the natural tooth. The brightness of all experimental groups was similar to that of the shade guide sample. Conclusion: Yttrium added to Ni-Cr alloys showed similar CIE L^*, a^*, b^* values to Ni-Cr alloys that did not contain yttrium, indicating that yttrium had no effect on color in metallic ceramic systems.

• Journal of Dental Rehabilitation and Applied Science
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• v.38 no.3
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• pp.178-188
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• 2022

Interim immediate denture is fabricated to minimize the period of edentulousness after removal of the patient's remaining teeth and before delivery of final prosthesis. In the case of using the CAD/CAM system, there is an advantage in that the manufacturing process in the clinic and laboratory can be simplified by overcoming some of the limitations of manufacturing interim immediate dentures in the traditional way. However, there are also disadvantages in that errors occur in the process of transmitting information about the patient's intermaxillary relationship to the digital network of the laboratory, resulting in unstable occlusal relationships or non-esthetic prostheses. To overcome this problem, using the simple and accurate POP BOW system's occlusal plane digital transfer method, it was possible to fabricate an esthetic and functional 3D printed interim immediate denture after removal of the remaining upper and lower anterior teeth.

• Journal of Technologic Dentistry
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• v.35 no.1
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• pp.43-48
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• 2013

Purpose: This study aimed to investigate the shear bond strength for non-precious alloy castings without beryllium, which has been used repeatedly for economical reason. Methods: The Schmitz-Schulmeyer test method was used to evaluate the shear bond strength between the non-beryllium Ni-Cr alloy Vera Bond 2V(AlbaDent, Inc. USA) and the Ceramco 3(Dentstply, York, PA, USA) porcelain powder. The maximum loading and shear bond strength were measured. The average shear strength(MPa) was analyzed with the one-way ANOVA and the Tukey's test( =.05). The fracture specimens were examined using Microscope to determine the failure pattern. Results: The mean shear bond strengths(SD) in MPa were group A(100% new metal) control 28.72(3.31); group B(50% new + 50% reused) 27.28(1.13); group C(all reused) 26.61(5.47). Microscope examination showed that group A and B specimens presented mixed failure, and group C specimens showed adhesive failure. Conclusion: In conclusion, forward this non-precious alloy dose not contain beryllium for how should use a more systematic study and for future advanced research is performed giving effect to be considered desirable.

• The Journal of Advanced Prosthodontics
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• v.9 no.6
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• pp.463-469
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• 2017

PURPOSE. To evaluate the fit of a three-unit metal framework of fixed dental prostheses made by subtractive and additive manufacturing. MATERIALS AND METHODS. One master model of metal was fabricated. Twenty silicone impressions were made on the master die, working die of 10 poured with Type 4 stone, and working die of 10 made of scannable stone. Ten three-unit wax frameworks were fabricated by wax-up from Type IV working die. Stereolithography files of 10 three-unit frameworks were obtained using a model scanner and three-dimensional design software on a scannable working die. The three-unit wax framework was fabricated using subtractive manufacturing (SM) by applying the prepared stereolithography file, and the resin framework was fabricated by additive manufacturing (AM); both used metal alloy castings for metal frameworks. Marginal and internal gap were measured using silicone replica technique and digital microscope. Measurement data were analyzed by Kruskal-Wallis H test and Mann-Whitney U-test (${\alpha}=.05$). RESULTS. The lowest and highest gaps between premolar and molar margins were in the SM group and the AM group, respectively. There was a statistically significant difference in the marginal gap among the 3 groups (P<.001). In the marginal area where pontic was present, the largest gap was $149.39{\pm}42.30{\mu}m$ in the AM group, and the lowest gap was $24.40{\pm}11.92{\mu}m$ in the SM group. CONCLUSION. Three-unit metal frameworks made by subtractive manufacturing are clinically applicable. However, additive manufacturing requires more research to be applied clinically.

• Molecules and Cells
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• v.37 no.7
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• pp.562-567
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• 2014

Human Hertwig's epithelial root sheath/epithelial rests of Malassez (HERS/ERM) cells are epithelial remnants of teeth residing in the periodontium. Although the functional roles of HERS/ERM cells have yet to be elucidated, they are a unique epithelial cell population in adult teeth and are reported to have stem cell characteristics. Therefore, HERS/ERM cells might play a role as an epithelial component for the repair or regeneration of dental hard tissues; however, they are very rare population in periodontium and the primary isolation of them is considered to be difficult. To overcome these problems, we immortalized primary HERS/ERM cells isolated from human periodontium using SV40 large T antigen (SV40 LT) and performed a characterization of the immortalized cell line. Primary HERS/ERM cells could not be maintained for more than 6 passages; however, immortalized HERS/ERM cells were maintained for more than 20 passages. There were no differences in the morphological and immunophenotypic characteristics of HERS/ERM cells and immortalized HERS/ERM cells. The expression of epithelial stem cell and embryonic stem cell markers was maintained in immortalized HERS/ERM cells. Moreover, immortalized HERS/ERM cells could acquire mesenchymal phenotypes through the epithelial-mesenchymal transition via TGF-${\beta}1$. In conclusion, we established an immortalized human HERS/ERM cell line with SV40 LT and expect this cell line to contribute to the understanding of the functional roles of HERS/ERM cells and the tissue engineering of teeth.

• The Journal of Advanced Prosthodontics
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• v.7 no.4
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• pp.312-316
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• 2015

PURPOSE. This study aimed to present the clinical applicability of restorations fabricated by a new method, by comparing the bond strength of between ceramic powder with different coefficient of thermal expansion and alloys fabricated by Selective laser sintering (SLS). MATERIALS AND METHODS. Fifty Co-Cr alloy specimens ($25.0{\times}3.0{\times}0.5mm$) were prepared by SLS and fired with the ceramic ($8.0{\times}3.0{\times}0.5mm$) (ISO 9693:1999). For comparison, ceramics with different coefficient of thermal expansion were used. The bond strength was measured by three-point bending testing and surfaces were observed with FE-SEM. Results were analyzed with a one-way ANOVA (${\alpha}$=.05). RESULTS. The mean values of Duceram Kiss ($61.18{\pm}6.86MPa$), Vita VM13 ($60.30{\pm}7.14MPa$), Ceramco 3 ($58.87{\pm}5.33MPa$), Noritake EX-3 ($55.86{\pm}7.53MPa$), and Vintage MP ($55.15{\pm}7.53MPa$) were found. No significant difference was observed between the bond strengths of the various metal-ceramics. The surfaces of the specimens possessed minute gaps between the additive manufactured layers. CONCLUSION. All the five powders have bond strengths higher than the required 25 MPa minimum (ISO 9693); therefore, various powders can be applied to metal structures fabricated by SLS.