• 제목/요약/키워드: Dental isolation

검색결과 82건 처리시간 0.029초

패독산류의 구강편평세포암종 및 악성중피종에 대한 항암 활성 (Anticancer Effect of Paedoksans for Oral Squamous Cell Carcinoma and Malignant Pleural Mesothelioma)

  • 오하나;김현정;채정일;심정현;윤구
    • 생약학회지
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    • 제48권3호
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    • pp.213-218
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    • 2017
  • In order to search for anticancer agent as therapy of oral squamous cell carcinoma (OSCC) and malignant pleural mesothelioma (MPM) from Korean traditional prescriptions, we selected 58 traditional prescriptions based on a review of the Korean traditional medicine books. Among the selected traditional prescriptions, only water extracts of paedoksan (敗毒散) showed relatively good cytotoxicity at the concentration of $50{\mu}g/ml$. Additionally, we evaluated cytotoxicity for various paedoksans and each herbal ingredient in paedoksans. The root of Anthriscus sylvestris exhibited more cytotoxic effect than any other ingredients in paedoksans. Bioactivity-guided fractionation of the MC layer of Anthriscus sylvestris led to the isolation of deoxypodophyllotoxin (DPT). DPT exhibited dose-dependently significant cytotoxicity against OSCC and MPM cell with nM range. Therefore, DPT from A. sylvestris might be a potential candidate as an effective anticancer therapeutic agent for OSCC and MPM.

고삼으로부터 항우식활성 물질의 분리 (Isolation and Identification of Anticariotic Compound from Sophora flavescens Ait.)

  • 이현옥;한동민;백승화
    • 한국미생물·생명공학회지
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    • 제30권4호
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    • pp.420-424
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    • 2002
  • 고삼으로부터 항우식활성물질을 탐색하기 위하여 고삼의 에틸 아세테이트 추출물을 이용하여 치아우식의 주요한 원인 균인 Streptococcus mutans에 대한 항세균효과와 Glucosylransferase 활성억제효과를 파악하였다. S. mutans에 대한 항세균효과는 3차분획의 소분획에서 S. mutans에 대한 최소억제농도가 3.13 $\mu\textrm{g}$/ml으로 나타나 높은 항세균효과를 관찰할 수 있었다. 또한 고삼의 에틸 아세테이트추출물은 glucan의 형성을 저해하였으며 100$\mu\textrm{g}$/ml의 농도에서 77%의 저해율이 나타났고, 통계학적으로 유의성이 인정되었다(p<0.05). 항우식활성물질을 나타내는 성분을 찾기위하여 고삼의 에틸 아세테이트 추출물을 recycling prep-HPLC 방법과 TCL, LR, $^1$H-NMR, $^{13}$C-NMR의 분광화학적인 자료를 이용하여 (2S)-2'-methoxy kurarinone와(+)-kurarinone을 동정하였다.

한국형 Streptococcus mutans의 분리 및 동정 (Isolation and Identification of Korean type Streptococcus mutans)

  • 현성희;장성렬;최영길
    • 미생물학회지
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    • 제27권3호
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    • pp.250-258
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    • 1989
  • 한국사람으로부터 치마우식증의 원인규능로 알려진 S. mutans를 분리, 동정하였고, 이들과 기존의 실험실균주간의 생리 및 생화학적 특성과 이들이 합성하는 다당류의 구성당을 비교하였다. 분리배지인 MS. MST와 선택배지인 MSP. MSPT. MSB 그리고 MSBT에서 모두 생장하고, mannitol dehydrogenase를 분비하는 균주 108, 110 및 120이 S. mutans로 분리, 동정되었다. 분리균주와 기존의 실험실균주간의 생리 및 생화학적 특성을 비교한 결과, 본 연구에서 분리한 3균주 모두 기존의 실험실균주와는 달리 hippuraterktnqnsgogyth를 가지고 있었다. 또한 균주 108은 lactose 발효 능력이, 균주 110은 sorbitol, lactose, inulin, melibiose, raffinose 발효능력이 , 균주 120은 inulin. raffinose 발효능력이 없는 것으로 나타나 기존의 실험 실균주와는 물론 이들 각각도 다른 생리 및 생화학적 특성을 나타내었다. 상기의 결과와 같이 분리균주 108, 110 그리고 120은 생리, 생화학적 특성 및 자당으로부터 합성되는 glucan의 양에 있어서도 기존의 실험실균주와 다르게 나타나 이들을 각각 한국형 S. mutans KHC108, KHCH10, KHC120이라 명명하였다.

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아말감과 이종(異種)금속의 거리에 따른 부식에 대한 고찰 (A Study on Corrosion according to Distance between Amalgam and Dissimilar Metals)

  • 김주원;정은경
    • 치위생과학회지
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    • 제4권3호
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    • pp.103-109
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    • 2004
  • 본 실험은 제조회사 매뉴얼과 통법에 의해서 Amalgam 합금, Ni-Cr alloy의 Crown용 Verabond, Denture용 Talladium $^{TM}alloy$로 각각 24개의 총 72의 시편을 하악 제1대구치근 원심 치관 폭경과 임상에서의 MOD cavity를 고려하여 제작하였고 인공 타액 80ml를 담은 200ml 용 비이커에 시편을 넣어 0mm, 7mm, 40mm 거리에서 7일 후 갈바닉 부식을 측정하였다. 유리금속은 유도 플라즈마 방출 분광기(Inductively Coupled Plasma - Atomic Emission Spectrometer(ICP-AES, JY-50P, VG Elemental Co. France)로 전해액내의 Cu, Ag, Ni, Cr, Sn, Zn, Hg를 정량 분석했으며 그 결과 다음과 같은 결론을 얻었다. 첫째, Cu, Sn, Ag, Hg, Zn은 아말감이 금 합금과 접촉했을 때가 크라운용 Ni-Cr alloy와 덴쳐용 Talladium alloy 보다 아주 많이 유리 했으며 금 합금이 구강조직과 생체 적합성이 가장 좋다지만 아말감과 함께 있을 때 가장 불리 했슴을 알 수 있었다. 둘째, 아말감이 금 합금과 접촉했을 때, 금 합금의 조성에서 Ni, Cr 같은 중금속이 함유되지 않았기 때문인지 전혀 유리되지 않았으나 Sn은 조성에는 없었지만 $227.1{\pm}18.0035{\mu}g/cm^2$나 유리 되었고 Hg도 유리되었는데 이는 아말감 자체의 유리 물질임을 추측할 수 있었다. 셋째, 아말감 합금과 금 합금 사이에서의 0mm, 7mm, 40mm 거리에서는 Cu, Ag는 유의성이 있었으며 Hg는 유의성이 없었다. 이는 금합금은 절대 아말감과 같이 사용해서 안되며 이종 보철물 사이의 거리에 관계없이 사용을 금해야 하는 것으로 사료된다. 넷째, 아말감합금이 Crown용 Ni-Cr 합금과 접촉했을 때 아말감의 Ag이 유리 되지 않았으며 Zn, Ni, Sn, Hg, Cu의 순으로 유리되었다. 0mm, 7mm, 40mm 거리에 따라서 유의성이 있었다. Hg는 유리 되지 않았지만 중금속인 Ni, Cr은 유리되었고 반대 악궁이나 거리가 떨어져 있으면 접촉보다 Hg의 유리가 적었다. 다섯째, 아말감합금이 Denture용 Talladium alloy 합금과 접촉했을 때 0mm, 7mm, 40mm 거리에서도 유의성이 있었다. 0mm, 7mm, 40mm 거리에 따라서 유의성이 있었다. Hg는 유리 되지 않았지만 중금속인 Ni, Cr은 유리되었고 반대 악궁이나 거리가 떨어져 있으면 접촉보다 Hg의 유리가 적었다. 여섯째, 인공 타액에서 접촉 시 Amalgam alloy와 Gold, Verabond, Talladium alloy의 Cu, Sn, Ag, Hg, Zn, Ni, Cr의 ICPES 검사 결과 Cu, Hg가 유의성을 있었다. 일곱째, 인공타액에서 아말감합금과 두 비귀금속인 Ni-Cr alloy(crown용), Denture용 Talladium alloy가 접촉한 경우 거리에 따른 Hg, Ni, Cr의 유리 부산물에서 유의성을 확인했다.

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PCR을 이용한 치아우식증 및 치주염 연관 병원체의 빠른 검출 (Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method)

  • 김재환;김미아;이대우;백병주;양연미;김재곤
    • 대한소아치과학회지
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    • 제41권4호
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    • pp.292-297
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    • 2014
  • 구강 병원체의 검출 방법은 여러 가지가 있지만 그 중 PCR을 이용한 검출이 확실하고 빠른 방법으로 알려져 있다. PCR을 위한 많은 DNA 추출법이 사용되고 있으나 상업적인 DNA 추출 kit들은 일반적으로 가격이 비싸고 절차가 여러 단계로 되어있으며, 그 외의 방법은 페놀과 클로로포름과 같은 유해한 화학물질을 써야하는 등의 단점이 있다. 이 연구에서 NaOH 용액을 이용한 개선된 DNA 추출 방법은 치아우식증, 치주염과 관련된 병원체를 빠르고 간단하며 비용-효율적으로 검출하였다. 세균으로부터 DNA를 추출하기 위한 boiling은 기존의 10분이 아닌 1분으로 충분하였고 $4^{\circ}C$에서 최소 13개월 이상 DNA의 보관이 가능하였으며 sonication 유무에 따른 차이는 없었다. 따라서, 이 방법은 상업적인 kit나 유해한 화학물질을 쓰지 않고서도 타액 표본으로부터 직접적으로 빠른 시간 내에 DNA를 추출하여 병원체의 유무 결과를 확인하는데 매우 적합할 것으로 생각한다.

미니돼지에서 다능성 피부유래 전구세포의 추출과 이의 다배엽 세포로의 분화유도에 대한 연구 (ISOLATION OF PORCINE MULTIPOTENTIAL SKIN-DERIVED PRECURSOR CELLS AND ITS MULTILINEAGE DIFFERENTIATION)

  • 최문정;변준호;강은주;노규진;김종렬;김욱규;박봉욱
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제34권6호
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    • pp.588-593
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    • 2008
  • There are increasing reports regarding regeneration of the defected tissues using tissue engineering technique. In this technique, multipotential stem cells are essential. There are many potential sources of adult stem cells, such as bone marrow, umbilical cord blood, fat, muscle, dental tissues and skin. Among them, skin is highly accessible and easily obtained with a minimum of donor site complications. Moreover, skin is an abundant adult stem cell sources and has the potential for self-replication and immune privilege. In this study, we isolated skin-derived precursor cells (SKPs) from the ear of adult miniature pigs. In these SKPs, the expression of transcriptional factors, Oct-4, Sox-2, and Nanog were detected by RT-PCR. In vitro osteogenesis and adipogenesis were observed at 3 weeks after transdifferentiations as assayed by positive von Kossa and Oil-red O staining, respectively. In addition, expression of osteocalcin and osteonectin in the osteogenic differentiation medium and $PPAR{\gamma}2$ and aP2 in the adipogenic differentiation medium were detected by RT-PCR. In vitro neurogenesis of porcine SKPs was observed during 24 and 72 hours after treatment of neurogenic differentiation medium. The results of this study suggest that SKPs demonstrate the properties of pluripotence or multipotence and multi-lineage differentiation. This indicates that autogenous SKPs are a reliable and useful source of adult stem cells for regenerative medicine.

Identification of Enterococcus faecalis on MSB Medium Selective for Mutans Streptococci

  • Lee, Seung-Hoon;Yoo, So-Young;Kim, Hwa-Sook;Kang, Sook-Jin;Lim, Sung-Hoon;Kim, Kwang-Won;Park, Jung-Min;Shin, Yong-Kook;Shin, Jeong-Hwan;Baek, Dong-Heon;Choe, Son-Jin;Kook, Joong-Ki
    • International Journal of Oral Biology
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    • 제31권1호
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    • pp.7-10
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    • 2006
  • Mitis-salivarius sucrose bacitracin(MSB) medium is widely used in the selective isolation of mutans streptococci(MS), a designation for a group of oral cariogenic species. Recently, we have isolated three bacterial strains grown on MSB agar from human dental plaques. The three strains exhibited biochemical characteristics similar to those of the biotype IV of MS, with the exception that they manifested a positive reaction for arginine deaminase. The objective of this study was to identify and characterize these three clinical isolates. The bacteria were identified with biochemical tests as well as by 16S rDNA cloning and sequencing. In order to compare the antibiotics susceptibility of the clinical isolates with that of type strain, the minimum inhibitory concentrations of 9 antibiotics were determined using broth dilution assays. The results identified all of our three clinical isolates as Enterococcus faecalis. All E. faecalis strains were found to be susceptible to penicillin G, amoxicillin, augmentin, and vancomycin, but were resistant to ciprofloxacin, cefuroxim axetil, and clindamycin. Our findings indicate that E. faecalis is capable of growing on MSB agar, and suggest that the MSB medium be improved so that only MS should be recoverable on the medium, as originally devised for their selection.

치아우식증환자와 치아정상인(齒牙正常人)의 치태(齒苔)에서 분리(分離)한 Streptococcus mutans의 혈청형(血淸型) 분포(分布)에 관(關)한 조사(調査) 연구(硏究) (A Survey of the Serotype Distribution of Streptococcus mutans Isolated from Dental Plaques of Caries-active and Caries-free Subjects)

  • 이진용;최유진;하윤문
    • 대한미생물학회지
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    • 제18권1호
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    • pp.23-29
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    • 1983
  • An epidemiological survey was carried out to identify the serotypes of Streplococcus mutans isolated from carious lesions of 65 caries-active subjects(CAS) and sound tooth surfaces of 40 caries-free subjects(CFS). The autoclaved antigen extract was performed on each isolate, and then, serotypes of the isolates were determined in agar-gel immunodiffusion test. The results were as follows: 1. S. mutans was found in 78% of the samples of CAS, and of CFS. The difference of isolation frequency between CAS and CFS was not observed. 2. Only one serotype per single subject was detected in 61% of total samples, in remaining 39% of samples two or more serotypes were detected. 3. In 41.2% of CAS samples plural serotypes of S. mutans were found, whereas 35.5% of CFS samples showed plural serotypes distribution. 4. The most frequently identified serotype in each subject was serotype c; 69.5% of subjects harbored serotype c S. mutans. Serotype d was next most frequently isolated from subjects, comprising 23.2%. 5. Serotype c strain was found in 64.7% of CAS, 77.4% of CFS. 6. Of the isolates from CAS and CFS, serotype c was most commonly found, comprising 48.8%, serotype d was found in 16.3%, serotypes f. e, and g comprising 13.2%, 9.3%, and 7.8% respectively. Serotypes a and b were also found but in far lower frequencies(2.3%, 0.8%). 7. Serotype c strains were more found in CFS than in CAS, but serotypes d and e were more found in CAS.

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Conservative approach of a symptomatic carious immature permanent tooth using a tricalcium silicate cement (Biodentine): a case report

  • Villat, Cyril;Grosgogeat, Brigitte;Seux, Dominique;Farge, Pierre
    • Restorative Dentistry and Endodontics
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    • 제38권4호
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    • pp.258-262
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    • 2013
  • The restorative management of deep carious lesions and the preservation of pulp vitality of immature teeth present real challenges for dental practitioners. New tricalcium silicate cements are of interest in the treatment of such cases. This case describes the immediate management and the follow-up of an extensive carious lesion on an immature second right mandibular premolar. Following anesthesia and rubber dam isolation, the carious lesion was removed and a partial pulpotomy was performed. After obtaining hemostasis, the exposed pulp was covered with a tricalcium silicate cement (Biodentine, Septodont) and a glass ionomer cement (Fuji IX extra, GC Corp.) restoration was placed over the tricalcium silicate cement. A review appointment was arranged after seven days, where the tooth was asymptomatic with the patient reporting no pain during the intervening period. At both 3 and 6 mon follow up, it was noted that the tooth was vital, with normal responses to thermal tests. Radiographic examination of the tooth indicated dentin-bridge formation in the pulp chamber and the continuous root formation. This case report demonstrates a fast tissue response both at the pulpal and root dentin level. The use of tricalcium silicate cement should be considered as a conservative intervention in the treatment of symptomatic immature teeth.

팬데믹 상황에서 음압병실 근무 간호사의 COVID-19 환자 간호 경험 (Nurses' Experience with Caring for COVID-19 Patients in a Negative Pressure Room Amid the Pandemic Situation)

  • 노은영;채영준;김현정;김은진;박연환
    • 대한간호학회지
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    • 제51권5호
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    • pp.585-596
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    • 2021
  • Purpose: The purpose of this study was to explore nurses' experience with caring for COVID-19 patients in a negative pressure room amid the spread of the pandemic. Methods: This study was a qualitative research, and focus group interviews were used to collect data. Three focus groups comprising 19 nurses were interviewed from February 17 to 25, 2021. All interviews were recorded and transcribed verbatim with the consent of the participants. The verbatim transcripts were scrutinized using thematic analysis. Results: Two main themes emerged from the analysis: 'Struggling in an isolated space' and 'Limitations of nursing infrastructure and system'. The nurses caring for COVID-19 patients experienced anxiety and fear about the infection, physical exhaustion, emotional burnout, and a sense of duty as a nurse. They also acknowledged the lack of guidelines, increased task and burden, limitations of nursing care, and the demand for improving the limitations of the nursing system. Conclusion: The results of this study demonstrate that nurses caring for COVID-19 patients encounter physical and emotional problems within the limited healthcare system. The study suggests that comprehensive interventions are needed for nurses. Furthermore, detailed guidelines, strengthening of nursing personnel, and improvements to the nursing system are vital to effectively cope with the pandemic. The government and medical institutions should be aware of the needs of nurses and what they are going through, and make efforts to improve the quality of life of healthcare workers and create a safe healthcare environment.