• Journal of Periodontal and Implant Science
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• 제28권4호
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• pp.691-703
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• 1998

The 16S rRNA analyzing method is a bacterial identification method that is useful in identifying bacteria which is difficult to do by other means. The following 7 types of bacteria which are Treponema, A. actinomycetemcomitans, P. gingivalis, Fusobacterium, B. forsythus, P. intermedia, P. micros were evaluated in order to study their distribution among patients with adult periodontitis. The 16S rRNA analyzing method was used to compare bacterial distribution among 3 groups. Subgingival plaque acquired from the affected sites(pocket depth ${\geq}6mm$) of 29 patients with adult periodontitis were grouped as the experimental group while plaque from the non-affected sites(pocket depth ${\leq}3mm$) were grouped as control 2 and finally plaque acquired from students with healthy periodontal tissues were grouped as control 1. The results are as follows ; 1. The distribution of Treponema was 12.5% for control 1, 21.4% for control 2 and 75.4% for the experimental group. For A. actinomycetemcomitans the distribution was 0.5%, 19.0%, 44.4% in respect to the order of groups mentioned above. P.gingivalis showed 10.5%, 43.1%, 94.0% distribution, Fusobacterium 33.0%, 48.3%, 81.0% distribution, B. forsythus 9.5%, 17.2%, 65.9% distribution, P. intermedia 1.0%, 12.1%, 26.3% distribution and finally P. micros 5.0%, 19.0%, 48.7% respectively. In all 7 types of bacteria, the experimental group showed higher bacterial distribution compared to the other two groups with statistically significant difference. 2. In the case of Treponema, A. actinomycetemcomitans, gingivalis,Fusobacterium, B. forsythus, P. intermedia, P. micros showed significant difference between control 1 and 2. These results suggest that the 16S rRNA analyzing method which was applied on Koreans for the first time could be utilized and useful in finding potential pathogens of periodontal disease.

• 한국융합학회논문지
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• 제10권2호
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• pp.21-28
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• 2019

본 연구는 진동회전 방식의 전동칫솔의 진동, 회전에 따른 치면세균막 제거율을 실험하여 전동칫솔 선택의 폭을 넓히고자 하였다. 진동회전타입의 전동칫솔 중 진동회전수가 다른 칫솔 3가지를 선정하였다. A: Oral-B D12(7,600회 회전), B: Oral-B D16(20,000회 진동, 7,600회 회전), C: Oral-B D20(40,000회 진동, 8,800회 회전). 세가지 칫솔의 세정력을 확인하기 위해 인공치면세균막을 치아에 1회, 2회, 3회 코팅하여 각 10회씩 2초간 세정하였다. 실험 후 잔여치면세균막를 촬영하여 Image J 프로그램을 활용하여 분석하였다(p<0.05). 연구에 따르면, 코팅한 모든 치면의치면세균막 제거율이 C칫솔이 가장 높았다. 연구결과를 토대로 치과종사자는 구강위생환경을 평가 한 후, 구강상태에 따른 적절한 칫솔의 추천과 교육이 필요할 것으로 사료된다.

• 대한미생물학회지
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• 제18권1호
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• pp.11-21
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• 1983

Various investigations have been carried out to elucidate the causative relationship between specific oral bacterial species and dental caries since it was first demonstrated that selected streptococoal species produced dental caries in germfree rats when fed a high-sucrose diet. Now, S. mutans is considered to play an important role in the development of dental caries in animals and humans, and only a limited number of species of bacteria other than S. mutans are occasionally found to be cariogenic in experimental animals. In this regard, association of the number of S. mutans in approximal plaque with caries experience (DMFT) was studied from 137 Korean school children($10{\sim}11$ year old). Biotypes of the collected strains of S. mutans were determined, and their relationship with caries status was also examined. The following results were emerged from the study. 1. S. mutans was detected in the plaques of all children. 2. Statistically significant positive correlation(r=0.445, p<0.001) was found between the caries experience(DMFT) and the number of S. mutans in approximal plaques. 3. The number of S. mutans were significantly higher(p<0.001) in plaques removed from carious surface than from sound surface. 4. The most frequent biotype was biotype I(78.8%), followed by IV(33.1%) and V(09.5%). Biotype II was isolated in the plaque of two children(1.7%) only. 5. There was no apparent relation of specific biotypes to carious status.

• 한국치위생학회지
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• 제23권1호
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• pp.43-53
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• 2023

Objectives: We developed an integrated cognitive function improvement program comprising cognitive, emotional, and physical domains, and remotely applied it to middle-aged adults to investigate its effects on oral health, cognitive function, and mental health improvement. Methods: The experimental group underwent the program remotely, using the Zoom platform. A total of 24 participants were recruited and divided into 12 experimental and 12 control groups. The program comprised cognitive, emotional, and physical activities. The sessions lasted 90 min and were performed twice a week for 6 weeks from April to May 2022. Results: Cognitive function, arousal, physical, and mental stress were significantly improved in the experimental group after the intervention than at the baseline (p<0.05). Regarding oral health, tongue plaque decreased 1.34-fold (p<0.01) and saliva increased 1.04-fold (p<0.05) in the experimental group after the intervention than at the baseline. Moreover, the experimental group showed significant improvements in tongue plaque and saliva than the control group (p<0.05 for tongue plaque and p<0.01 for saliva). Regarding mental health, social support significantly increased 11.67-fold (p<0.05) in the experimental group than at the baseline. The experimental group also showed significantly improved social support than the control group (p<0.01). Conclusions: The non-face-to-face integrated cognitive function improvement program for middle-aged adults improved their cognitive function and oral and mental health. Based on these findings, this program may be a useful health program tool for middle-aged individuals.

• 한국식품위생안전성학회지
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• 제37권2호
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• pp.107-113
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• 2022

현재까지, 식이 당의 전체적 또는 부분적 대체당으로서 자일리톨의 치아우식증에 대한 예방 효과를 확인하기 위해 많은 연구가 수행되었다. 본 총설논문에서는 자일리톨의 구강건강에 대한 기존 연구들을 바탕으로 자일리톨의 치아우식증 예방 기전, 치아우식증 예방, 치아우식증 유발균의 모자전염 예방, 노인의 구강건강에 미치는 영향 등이 정리되었다. 식품에 함유된 탄수화물 및 식이 당은 구강 내에서 산을 생성하는 미생물에 의해 발효되어 치석 및 산을 생성하여 치아우식증을 일으킨다. 그러나 충치를 일으키는 세균 대부분은 자일리톨을 대사하여 산을 만들어 낼 수 없다. Streptococcus mutans (S. mutans)에 의한 비대사성 대사물로서 세포 내에 축적된 자일리톨은 S. mutans의 성장을 억제하고 치석 형성을 억제하여 치석과 타액 중 S. mutans 수를 감소시킨다. 또한, 자일리톨은 치아의 재광화(remineralization) 과정에 작용한다. 자일리톨은 치아우식증 예방, S. mutans의 모자감염 예방, 노인의 충치예방 및 타액분비 증가에 효과가 있는 것으로 확인되었다. 결론적으로, 자일리톨은 남녀노소 구분 없이 구강건강에 효과적이며 쉽게 섭취할 수 있는 장점이 있다. 향후, 자일리톨의 과량섭취에 따른 부작용과 구강 및 장내 환경에 미치는 전반적인 영향에 대한 보다 정확하고 상세한검토가 필요하다.

• International Journal of Oral Biology
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• 제33권4호
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• pp.197-203
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• 2008

It has been reported that the antimicrobial susceptibility patterns of viridans streptococci vary according to geographical region. Although several studies on the antibiotic resistance of viridans streptococci in foreign countries have been reported, little is known about the distribution of resistance among viridans streptococci in Korea. In this study, 88 isolates of viridans streptococci from Korean students' dental plaque were identified as 12 different species. The susceptibility of these isolates to 8 antibiotics was investigated. The in vitro antibiotic activity of penicillin G, ampicillin, vancomycin, streptomycin, gentamicin, erythromycin, amoxicillin, and tetracycline was measured by the broth microdilution method. The range of the minimum inhibitory concentrations (MIC), MIC50, MIC90, and the percentage of the susceptible isolates were determined. Streptococcus mutans and Streptococcus salivarius were susceptible to the 8 antibiotics. Isolates with resistance to vancomycin, streptomycin, and amoxicillin were not found. The overall resistance rates of the 88 isolates to penicillin G, ampicillin, gentamicin, erythromycin, and tetracycline were 12.5%, 62.5%, 62.5%, 26.1%, and 26.1%, respectively.

• 한국치위생학회지
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• 제8권2호
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• pp.13-22
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• 2008

The purpose of this study is to analyze the correlation between cause and the element of mouth odor through measurement and identification of mouth odor and is to apply to effective method for elimination of halitosis, Subjects were 150 people who visited Dong Pusan College and lived in Pusan Cross-sectional survey was used and mouth odor was measured by Oralchroma. Oral examination proceeded with status of tongue plaque distribution and calculus index, Statistical analysis was performed with SPSS version 13.0 for window Among 150 people, 67.3% subjects thought they had halitosis, 89.3% people felt that their mouth odor were the severest as soon as wake-up, Tongue was serious place for halitosis by 53.3% subjects Methylmercaptan and dimethyl sulfide showed the highest correlation by 0.549 index score, Moreover, methyl mercaptan was observed the correlation with brushing time in 0.190 index score and with food intake in 0.177(P<0.05). In conclusion, brushing time and food intake were correlated with mouth odor.

• 한국치위생학회지
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• 제10권1호
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• pp.215-229
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• 2010

Objectives : This study was to assess the children's mean number of decayed or filled primary teeth in relation to their mother's oral health behavior and then to increase children's oral health. Methods : The 346 children and their mothers were selected for this study. The children were 4 or 7 years old in the 4 dental clinics in Busan and Kyungnam, Korea. Data were collected by examination on children and self-administrated questionnaire on their mothers. The questionnaire was surveyed mother's oral health behaviour and children experienced dental caries or not and the number of decayed or filled they had were used as outcome variables. Results : 1. The mother's education level affected children's dft index significantly(p<0.05), In the case of tooth brushing method of children, the group with circle teeth wipes shows the low dft index(p<0.01). 2. On the other hand the group with snack as food eaten between meals has high dft index(p<0.001). 3. The group with mother's visiting to dentist within recent 1 year, experience in removing plaque or willing to attend the oral health education show low dft index(p<0.05). The important variables affecting to dft index are experience with oral health education, tooth brushing guidance, replacement of toothbrushes, the kind of food eaten between meals, recent experience of plaque removal and willing to participating in the oral health class. Conclusions : This study showed that the mother's oral health behavior and concern play an important role for the prevention of preschooler's dental caries. Dental health education would be focused on the mothers, expecially for the practice of preventive behavior by preschools themselves.

• 한국학교보건학회지
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• 제27권1호
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• pp.1-6
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• 2014

Purpose: The purpose of this study was to investigate the effect of oral health education on adolescents. Methods: The subjects were middle school students in a health promoting school in Gunpo, Gyeonggi Province. Before giving the students oral health instruction, their modified Patient Hygiene Performance index (PHP index) was measured using disclosing solution. Then, the students were taught the right way to brush teeth (Rolling method) and use oral hygiene devices such as dental floss, tongue cleaner and interdental brush. Their modified PHP index was re-measured three days after and seven days after the education to evaluate the effect and persistency of oral health education. of the total 116 students, 48 students who completely filled out the questionnaire and participated in the whole process, photo shooting and three times of PHP index measurement, were included in the final analysis. Results: The students' modified PHP index significantly decreased three days after the education. However, the index slightly went back up seven days after the oral health education. Conclusion: Oral health education was effective in reducing dental plaque which remained after brushing. Continuous education about and constant motivation for oral hygiene turned out to be critical to maintain the impact of oral health education. Therefore, it is necessary to develop educational contents which can constantly motivate teenagers.

• Journal of Periodontal and Implant Science
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• 제24권2호
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• pp.340-356
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• 1994

The purpose of this study was to assess the clinical and microbiological effect of chlorhexidine rinse and supragingival plaque control following scaling and root planing on adult periodontal disease. 14 patients with adult periodontitis were selected for the study . They had not taken antibiotics for 6 months and history of dental treatment for 6 months before the study. Patients received a supragingival scaling and root planing under local anesthesia, plaque control group was subjected to professional plaque control 2 times for a period 2 week, chlorhexidine rinse group were subjected to twice daily 0.2% chlorhexidine rinse for a period 2 week. Clinical examination (plaque index, gingival index, probing pocket depth) and distribution of the bacteria morphology of subgingival plaque were monitored on baseline (0 week), 1 week, 2 week, 4 week and 6 week. The results were as follows : 1. Plaque index in chlorhexidine rinse group , plaque control group and control group was significantly reduced during all weeks (P<0.05). 2. Probing pocket depth was significantly reduced at 2, 4, 6 week (P<0.05) in chlorhexidine rinse group and control group, plaque control group was significantly reduced during all weeks (P<0.05). 3. Gingival index was significantly improved at 2, 4, 6 weeks(P<0.05) in chlorhexidine group and plaque control group, control group was significantly improved at 1, 2, 4 weeks (P<0.05). 4. Percentage of cocci was significantly increased at 1, 2, 4 and 6 weeks in chlorhexidine rinse group and control group, plaque control group was significantly increased at 2, 4 and 6 weeks(P<0.05). 5. Percentage of non-motile rods in all group were not significantly changed when compared with those of baseline (0 week) (P<0.05). 6. Percentage of motile rods was significantly reduced during all weeks (P<0.05) in chlorhexidine rinse group, plaque control group was significantly reduced at 2, 4, 6 weeks and 1, 2 and 4 weeks in control group. 7. Percentage of spirochetes was significantly reduced during all weeks (P<0.05), plaque control group was significantly reduced at 2, 4, 6 weeks and 1, 2, 4 weeks in control group. This results were suggested that clinical and microbiological effect of chlorhexidine rinse and supragingival plaque control following scaling and root planing on periodontal disease