• 한국수정란이식학회지
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• 제33권2호
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• pp.85-97
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• 2018

The trans-differentiation potential of mesenchymal stem cells (MSCs) is employed, but there is little understanding of the cell source-dependent trans-differentiation potential of MSCs into corneal epithelial cells. In the present study, we induced trans-differentiation of MSCs derived from umbilical cord matrix (UCM-MSCs) and from dental tissue (D-MSCs), and we comparatively evaluated the in vitro trans-differentiation properties of both MSCs into corneal epithelial-like cells. Specific cell surface markers of MSC (CD44, CD73, CD90, and CD105) were detected in both UCM-MSCs and D-MSCs, but MHCII and CD119 were significantly lower (P < 0.05) in UCM-MSCs than in D-MSCs. In UCM-MSCs, not only expression levels of Oct3/4 and Nanog but also proliferation ability were significantly higher (P < 0.05) than in D-MSCs. In vitro differentiation abilities into adipocytes and osteocytes were confirmed for both MSCs. UCM-MSCs and D-MSCs were successfully trans-differentiated into corneal epithelial cells, and expression of lineage-specific markers (Cytokeratin-3, -8, and -12) were confirmed in both MSCs using immunofluorescence staining and qRT-PCR analysis. In particular, the differentiation capacity of UCM-MSCs into corneal epithelial cells was significantly higher (P < 0.05) than that of D-MSCs. In conclusion, UCM-MSCs have higher differentiation potential into corneal epithelial-like cells and have lower expression of CD119 and MHC class II than D-MSCs, which makes them a better source for the treatment of corneal opacity.

• Transactions on Electrical and Electronic Materials
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• 제13권6호
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• pp.310-316
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• 2012

A new method for early caries diagnosis was proposed and tested through a home-made optical examination system that used quantitative light fluorescence (QLF) and digital imaging fiber optic transillumination (FOTI) (DIFOTI), with light sources across a wide spectral range, from 350 nm to 1,000 nm. The front-illuminated infrared light scattering image (FIR) showed similar diagnostic abilities to that of DIFOTI. The FIR method was invented based on the observation that caries lesions lose the high transmittance and low scattering properties of benign enamel tissue. There are various methods for the early diagnosis of caries, such as visual examination, exploration, X-ray radiography, QLF, FOTI, and infrared fluorescence (diagnodent). Among them, methods based on optical properties are regarded as having the most potential. A comparative study was performed between the FOTI, QLF, diagnodent, optical coherence tomography, and FIR scattering image methods, using 20 extracted teeth samples with early caries. A scale of lesion measurement based on optical image contrast was proposed. The statistical analysis showed a significant correlation between the DIFOTI and FIR methods (r = 0.35, p < 0.05). However, the QLF and diagnodent methods showed little association with FIR images, as they have different detection principles as compared with FIR. Tomographic images obtained by OCT, using 1,330 nm super luminescent LED as a gold standard of tooth structure, verified that the FOTI and FIR results correctly represented the lack of homogeneity in dental tissue. The newly proposed FIR method attained similar diagnostic results to those of FOTI, but with an easier approach.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제32권2호
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• pp.154-161
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• 2010

Many edentulous posterior maxilla are found to be compromised by alveolar resorption and increased pneumatization of the sinus. One of the surgical procedures to overcome this anatomical limitation is sinus floor elevation with bone graft, which is reported as more appropriate and more successful procedure. Commonly, if the residual bone height is over 5mm, sinus floor elevation is operated through transcrestal approach using osteotome technique. But, it is possible for patients to feel discomfort during operation and dizziness after operation while malleting, sinus floor elevation, using osteotome technique. Some instruments and methods has been used to overcome these problems and use more easily. The aim of this study is to compare between the surgical procedure of sinus floor elevation using Hatch reamer technique and that of sinus floor elevation using osteotome technique. From 2004 Feb to 2007 Oct, we investigate patients (osteotome group: 72, Hatch reamer group: 70) who were given implant surgery with sinus floor elevation (osteotome group: 92, Hatch reamer group: 98). We analysed gender, age, residual bone height, amount of sinus floor elevation, used graft material, total success rate, failure rate by residual bone height and implant type and discomfort during operation, etc. The results obtained were as follows. 1. In the amount of sinus elevation was osteotome group was $3.85{\pm}1.02\;mm$ and Hatch reamer group was $3.93{\pm}1.38\;mm$. There was no statistically significant difference between the two groups (P > 0.05). 2. At the total success rate, osteotome group was 92.4% and Hatch reamer group was 94.9%. There was no statistically significant difference between the two groups (P > 0.05). 3. On the discomfort during the operation by using numerical rating scale, osteotome group was $2.87{\pm}0.83$ and Hatch reamer group was $1.12{\pm}0.64$. There was statistically significant difference between the two groups (P < 0.05). The Hatch reamer group clinical results was similar to osteotome group and we thought that Hatch reamer technique can overcome the faults of osteotome technique.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제34권6호
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• pp.588-593
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• 2008

There are increasing reports regarding regeneration of the defected tissues using tissue engineering technique. In this technique, multipotential stem cells are essential. There are many potential sources of adult stem cells, such as bone marrow, umbilical cord blood, fat, muscle, dental tissues and skin. Among them, skin is highly accessible and easily obtained with a minimum of donor site complications. Moreover, skin is an abundant adult stem cell sources and has the potential for self-replication and immune privilege. In this study, we isolated skin-derived precursor cells (SKPs) from the ear of adult miniature pigs. In these SKPs, the expression of transcriptional factors, Oct-4, Sox-2, and Nanog were detected by RT-PCR. In vitro osteogenesis and adipogenesis were observed at 3 weeks after transdifferentiations as assayed by positive von Kossa and Oil-red O staining, respectively. In addition, expression of osteocalcin and osteonectin in the osteogenic differentiation medium and $PPAR{\gamma}2$ and aP2 in the adipogenic differentiation medium were detected by RT-PCR. In vitro neurogenesis of porcine SKPs was observed during 24 and 72 hours after treatment of neurogenic differentiation medium. The results of this study suggest that SKPs demonstrate the properties of pluripotence or multipotence and multi-lineage differentiation. This indicates that autogenous SKPs are a reliable and useful source of adult stem cells for regenerative medicine.

• 치과방사선
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• 제25권2호
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• pp.471-482
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• 1995

Bony remodeling pattern of condyle fractures in children are different from in adult for growing of condyle, also might affect treatment and prognosis of the condyle fracture. Subjects of this clinical and radiologic study were 26 temporomandibular joints diagnosed as condyle fracture in 23 patients under 15 years old age. They were treated with conservative method at Dental Hospital of Yonsei University from Jan., 1986 to Oct., 1994. Bony remodeling related with fracture pattern was evaluated. The results obtained are as follows: 1. The ratio of male to female in patients with condyle fracture was 1 : 0.9 and the difference of sex ratio was not noted. Comparing with preschool-age group and school-age group, age frequency was higher in preschool-age group(83％). 2. Fallen down(54％) was the most frequent cause of condyle fractures. Traffic accident and slip down were followed. 3. The most common clinical sign of condyle fractures was tenderness to paipation09 cases). Mouth opening limitation07 cases), swelling(7 cases), malocclusion(3 cases) were next in order. 4. According to sites of condyle fractures, unilateral fractures were in 20 patients and bilateral fractures in 3 patients, therefore total 23 patients-26 cases of condyle fracture were observed. According to fracture distribution, condyle fractures were in 10 patients(44％). Condyle fractures with symphysis fracture(9 patients, 39％), condyle fractures with ascending ramus fracture(2 patients, 9％), condyle fracture with mandibular body fracture(1 patient, 4％), and condyle fractures with mandibular angle fracture(1 patient, 4％) were followed. 5. In displacement pattern of fractured fragment of mandibular condyle, dispiacement(17 cases, 66％) was most common. Dislocation(5 cases, 19％) and deviation (4 cases, 15％) were next in order. 6. During the observation period of fractured condyles, remodeling patterns of fracture sites related with articular fossa were observed with usual condylar shape in 23 cases and with prominently different shape in 3 cases.

• 생명과학회지
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• 제29권8호
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• pp.895-903
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• 2019

이 연구는 단일 세포로 분리된 유도만능줄기세포(induced pluripotent stem cells, iPSCs)에 anoikis 세포사멸을 억제할 수 있는 Rho-associated protein kinase (ROCK)의 억제제를 처리하여 iPSCs의 세포 생존성을 향상하고자 하였다. Episomal plasmid 방법으로 확립된 iPSCs를 단일세포로 분리한 후, ROCK 억제제 Y-27632 dihydrochloride (Y-27632)를 0 uM, 0.5 uM, 1 uM, 2.5 uM, 5 uM, 7.5 uM 및 10 uM 농도별로 5주일 동안 각각 처리하였을 때, 5 uM 이상의 농도에서 세포의 생존율이 유의적으로 향상되었고, 10 uM의 Y-27632을 0일, 1일, 2일, 3일, 4일 및 5일 동안 처리하였을 때, Y-27632의 노출 기간이 길어질수록 세포의 생존율이 유의적으로 향상되는 것을 관찰하였다. 그러나, Y-27632의 노출 후, iPSCs의 형태학적 분화가 관찰되어 10 uM의 Y-27632에서 5일 동안 iPSCs에 처리 한 후, 줄기세포학적인 특성을 비교 조사하였다. 우선, octamer-binding transcription factor 4 (OCT-4), homeobox protein NANOG (NONOG) 및 SRY-box 2 (SOX-2) 줄기세포 특이 유전자의 발현은 Y-27632를 처리한 실험군은 Y-27632를 처리하지 않은 대조군에서 서로 유의적인 차이를 나타내지 않았다. 또한, Y-27632를 처리한 실험군은 Y-27632를 처리하지 않은 대조군과 비교하여 telomerase 활성과 이것의 활성과 관련된 telomerase reverse transcriptase (TERT) 및 telomerase RNA component (TERC)의 유전자 발현에는 유의적인 차이가 없었다. 이상의 결과로 보아, iPSCs에 Y-27632를 처리하였을 때, iPSCs의 줄기세포의 특정을 유지하면서 anoikis에 의한 세포사멸을 감소시켜 세포 생존율이 증가한다는 것을 알 수 있었다.

• Journal of Oral Medicine and Pain
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• 제37권4호
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• pp.243-250
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• 2012

임플란트 수술이나 발치 같은 침습적인 치과치료로 인한 삼차신경 손상의 증가는 최근 치과계의 가장 민감한 문제의 하나로서 향후 법적인 문제로 까지 이어질 수 있다. 그러므로 신경손상에 대한 객관적이고 신뢰성 있는 평가 즉, 정량적 감각신경 검사(quantitative sensory testing)의 필요성이 강조되고 있으며, 온도역치 검사는 QST의 필수 항목으로 구강영역에서도 신뢰성이 입증되어 있다. 국내에서는 삼차신경손상과 관련한 온도역치 검사에 대한 보고가 아직 없기에, 본원에서 시행한 일부 증례의 온도역치 검사의 결과를 평가하고자 하였다. 2011년 5월부터 2012년 10월까지 단국대학교 치과대학 부속 치과병원 구강내과에 내원한 삼차신경손상이 의심되는 환자중 온도역치 검사를 시행한 18명의 환자 자료를 분석하였다. 환자들의 냉인지역치(cold detection threshold, CDT)는 이환측이 비이환측에 비해 유의하게 낮았으나 온인지역치(warm detection threshold, WDT), 냉통각역치(cold pain threshold, CPT), 열통각역치(heat pain threshold, HPT)는 통계적 차이를 보이지 않았다. 향후 더 많은 피검자를 대상으로 온도 자극과 관련하여 감각저하나 감각과민, 통각저하나 통각과민 같은 유형별 비교 연구가 필요하다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제29권6호
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• pp.467-473
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• 2007

이 실험은 이식재로 Deproteinated Bovine Bone Powder (DBBP)를 사용하였을 때와 골 이식재를 사용하지 않고 단순히 Absorbable Gelatin Sponge(AGS)만을 사용하였을 때의 BMP4와 BMP6의 상대적인 발현량을 real-time RT-PCR을 이용하여 비교해 보기 위한 것으로 다음과 같은 결과를 얻었다. 1. BMP4의 경우 처음 1일째와 3일째의 경우 DBBP군과 AGS군 모두 일정하게 증가하였으나 5일째 AGS군에서 감소하다가 7일째 다시 증가하였으며 9일째 다시 감소하였다. DBBP군에서는 7일까지 계속하여 증가하다가 9일째 감소하였다. DBBP군이 AGS군에 비해 발현의 양이 많은 경우가 많았지만 유의성은 없었다 (p>0.05). 2 BMP6의 경우 처음 1일째와 3일째의 경우 DBBP군과 AGS군 모두 일정하게 증가하였으나 5일째 AGS군에서 감소하다가 7일째 다시 증가하였으며 9일째 다시 감소하였다. DBBP군에서는 7일까지 계속하여 증가하다가 9일째 감소하였다. AGS군이 DBBP군에 비해 발현의 양이 많은 경우가 많았지만 유의성은 없었다 (p>0.05). 3. 두 군간에 동일시기에 BMP발현이 유의할 만한 차이를 보이지 않는 것은 DBBP와 AGS 모두 space retainer로서 작용을 하여 혈병중의 BMP발현의 양상이 비슷하기 때문으로 여겨진다. 4. 따라서 DBBP가 AGS에 비해 초기 골재생에 크게 유리한 점은 없는 것으로 여겨지며 초기 골 형성에서 BMP의 발현은 이식재의 종류가 아니라 물리적인 carrier로서의 작용이 더 중요한 것으로 여겨진다.

• 치위생과학회지
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• 제4권1호
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• pp.21-25
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• 2004

현대사회에서는 생활환경의 변화와 교통사고의 증가에 의해 해마다 소아의 외상의 증가하고 있는데 구강조직에 대한 외상성 손상을 완전히 치단하는 데에는 한계가 있어 외상성 치아손상에 관한 실태를 조사 분석함으로써 외상처치와 예방에 필요한 기초자료를 제시하고자 강릉대학교 치과병원 소아치과에 2000년 10월 4일부터 2004년 2월 27일까지 외상을 주소로 내원한 113명의 환자를 대상으로 조사 분석한 결과 다음과 같은 결론을 얻었다. 1. 연구대상자의 성별에서는 2.05:1의 비율로 남자의 경우가 외상빈도가 더 높게 나타났다. 평균 연령은 남자 5.27세, 여자 5.27세로 차이가 없었고, 전체 연령중 2세에서 외상환자가 제일 많았다(21.2%) 2. 외상부위별 내원기간을 조사한 결과 유치는 당일(34.4%)에 내원한 경우가 가장 많았고, 영구치는 1일 후(38.8%)에 내원한 경우가 가장 많았다. 3. 외상부위별 치아손상의 원인을 분석한 결과 넘어진 경우가 유치 59.4%, 영구치 55.1%, 물체에 부딪친 경우 유치 26.6%, 영구치 26.5%로 나타나 대부분 넘어지거나 부딪쳐서 외상이 발생한 것으로 나타났다. 4. 성별에 따른 치아손상이 발생한 장소는 가정인 경우가 남자 42.1%, 여자 35.1%로 남녀모두 가정에서 외상이 가장 많이 발생하였다. 외상부위에 따른 치아손상 발생한 장소로는 유치는 가정(59.4%)에서 가장 많이 발생하였고, 영구치는 유치원 및 학교(28.6%)에서 가장 많이 발생하였다. 5. 외상부위에 따라 치아손상을 받은 치아의 수를 분석한 결과 유치는 2개(56.3%), 1개(31.3%), 3개와 4개 이상 각각(6.3%), 순으로 나타났으며, 영구치는 2개(46.9%), 1개(28.6%) 4개 이상(16.3%), 3개(8.2%) 순으로 영구치인 경우 4개 이상인 경우도 비교적 높게 나타났다. 6. 성별에 따른 치아손상이 발생한 부위로는 남자 유치 56.6%, 영구치 43.4%, 여자 유치 56.8%, 영구치 43.2%로 남녀 모두 유치에서 외상이 가장 많이 발생하였다. 7. 유치와 영구치의 치아손상부위는 상악 전치부가 거의 대부분을 차지하고, 유치(75%)와 영구치(63.8%) 모두 상악 중절치에서 외상율이 높게 나타났다. 8. 전체적인 외상성 치아손상에 따른 외상종류의 조사결과는 동요 30.2%, 치관파절 28.0%, 함입 14.7%, 진탕 8.9%, 완전 탈구 7.1%, 정출 2.2%, 변위 1.8% 순으로 나타났다. 유치와 영구치별로 외상성 치아손상의 종류를 분류하였을 때 유치는 동요(35.8%)가 가장 많이 나타났고, 파절에서 치관파절은 영구치(44.8%)에서 많이 발생하였다. 9. 외상부위에 따른 외상성 치아손상처치는 처치없이 경과관찰을 한 경우가 유치는 43.2%, 영구치 38.9%로 나타났으며, 치료한 경우 유치는 치수절제술(22.0%), 영구치는 임시적산부식레진수복(19.5%)을 가장 많이 사용하였다.

• 농촌의학ㆍ지역보건
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• 제4권1호
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• pp.20-30
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• 1979

To investigate the status of morbidity and medicare utilization during last 8 months from 1 st Oct. 1977 to 31th May, 1978 in the area under Sam-Wha Medicare Insurance Union, a study was carried out through analyzing the medicare records of patients who are enrolled. For the study, 3 doctors and one nurse were mobilized and the results are as follows: 1) The total number of the Medicare insurance Union members in the study area were 5,735 composed of 3,000 males(52.3%) and 2,735 females(47.7%). 2) The total number of patients were 1,405 composed of 783 males (55. 0%) and 622 females(45. 0%) and the incidence rate per 1,000 population was 245.0 of total(261.0 in males and 227.4 in females). 3) Five major diseases with 52, 7% of total patients were acute upper respiratory infection(20.7%), peptic ulcer(12.2%), bronchitis(5.5%), injuries(5.2%) and dental problems(5.1%). 4) The order of the incidence rate of age group per 1,000 population was the year group on 0-4(342.6), 25-44(312.7), 45-64(307.0), 65 and over(240.3), 15-24(178.8) and 5-14(164.8). 5) Of the 1,405 total patients, the out-patients were 1,661(96.9%) and the in-patients were 44(3.1%) and the ratio wae 30.9 : 1.0. 6) Among the out-patients 96.7% of them were cared in primary medicare facilities, 1.0,% in secondary care, and 2.3% in tertiary care. And among the in-patients 50. 5% of them were cared in primary medicare facilities, 4. 5% in secondary care, 45. 5% in tertiary care. 7) Duration of medicare was concentrated within a week in 84. 3% of total patients.