• Biomaterials and Biomechanics in Bioengineering
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• v.3 no.2
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• pp.105-114
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• 2016

The purpose of this study is to evaluate the effects of cryopreservation on dental pulp-derived stem cells (DPSC) viability over a period of three years. Dental pulp-derived stem cells were isolated and cultured from thirty-one healthy teeth. DPSC isolates were assessed for doubling-time and baseline viability prior to cryopreservation and were assessed again at three time points; one week (T1), 18 months (T2), and 36 months (T3). DPSC can be grouped based on their observed doubling times; slow (sDT), intermediate (iDT), and rapid (rDT). Viability results demonstrated all three types of DPSC isolates (sDT, iDT and rDT) exhibit time-dependent reductions in viability following cryopreservation, with the greatest reduction observed among sDT-DPSCs and the smallest observed among the rDT-DPSC isolates. Cryopreserved DPSCs demonstrate time-dependent reductions in cellular viability. Although reductions in viability were smallest at the initial time point (T1) and greatest at the final time point (T3), these changes were markedly different among DPSC isolates with similar doubling times (DTs). Furthermore, the analysis of various DPSC biomarkers - including both intracellular and cell surface markers, revealed differential mRNA expression. More specifically, the relative high expression of Sox-2 was only found only among the rDT isolates, which was associated with the smallest reduction in viability over time. The expression of Oct4 and NANOG were also higher among rDT isolates, however, expression was comparatively lower among the sDT isolates that had the highest reduction in cellular viability over the course of this study. These data may suggest that some biomarkers, including Sox-2, Oct4 and NANOG may have some potential for use as biomarkers that may be associated with either higher or lower cellular viability over long-term storage applications although more research will be needed to confirm these findings.

• Journal of Korean society of Dental Hygiene
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• v.9 no.1
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• pp.113-123
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• 2009

This study was undertaken evaluate the general image for dental hygienist. A questionnaire was given to each Dentist, the staff of dental. 65 Dentist, 145 the staff of dental were included in the subjects between Jul. 1 and Oct. 30, 2008. The collected data was analysed by the SPSS Win 12.0/PC using frequency, percentage, mean, standard deviation, chi-square, t-test, ANOVA. The image for the dental hygienist was analyzed by 28 items. As a result of analysis on image of dental hygienist, The Dentist's group and staff's group, both appreciated positive image that explain process of treatment to patient very well, having professional knowledge and experienced skill, carries on an effective and valuable work. give a confidenced to patient. These group also gave negative image in the aspect that the dental hygienist is stressful job, handmaid of Dentist, participate of decided something for dental clinic, having association activities. It is necessary for dental hygienist by oneself development ability through science congress variety major and acquirement new technology, exchange information, interchange activities, and association of dental hygienist should efforts for growing up image through mass media.

• The Journal of Korea Assosiation for Disability and Oral Health
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• v.10 no.2
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• pp.78-83
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• 2014

The objective of this study was to confirm the validity of classification of dental disability by measuring the dental treatment time required for disabled patients and identify the disability type that requires more of chair time. As a result of measuring a total of 123 patients who were admitted to the Seoul dental hospital for the disabled on Oct. 2014, I was able to make the following conclusions: 1. For dental counseling and check-up, the chair time did not show significant differences between the different types of disability. 2. For periodontal treatment, restorative treatment, root canal treatment, and surgical treatment, the difference of the chair time for dentally disabled patients was significantly longer than that of non-dentally disabled patients. 3. The difference in the total chair time for prosthetic treatment was statistically insignificant. However, each of the prosthetic treatment steps did show statistically significant differences.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2004.05b
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• pp.327-329
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• 2004

We developed the numerical analysis program using LabVIEW for the monte-carlo simulation code for OCT signal in dental medium. In calculation, we obtain the two different signals as a function of the probing depth. These two signals are very different in their angular distributions, and the numbers of scattering in dental medium. Signal 2 begins to exceed the signal 1 at a very small probing depth(=60${\mu}{\textrm}{m}$).

• International Journal of Oral Biology
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• v.35 no.4
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• pp.209-214
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• 2010

Cells that have endogenous multipotent properties can be used as a starting source for the generation of induced pluripotent cells (iPSC). In addition, small molecules associated with epigenetic reprogramming are also widely used to enhance the multi- or pluripotency of such cells. Skinderived precursor cells (SKPs) are multipotent, sphereforming and embryonic neural crest-related precursor cells. These cells can be isolated from a juvenile or adult mammalian dermis. SKPs are also an efficient starting cell source for reprogramming and the generation of iPSCs because of the high expression levels of Sox2 and Klf4 in these cells as well as their endogenous multipotency. In this study, valproic acid (VPA), a histone deacetylase (HDAC) inhibitor, was tested in the generation of iPSCs as a potential enhancer of the reprogramming potential of SKPs. SKPs were isolated from the back skins of 5-6 week old C57BL/6 X DBA/2 F1 mice. After passage 3, the SKPs was treated with 2 mM of VPA and the quantitative real time RT-PCR was performed to quantify the expression of Oct4 and Klf4 (pluripotency specific genes), and Snai2 and Ngfr (neural crest specific genes). The results show that Oct4 and Klf4 expression was decreased by VPA treatment. However, there were no significant changes in neural crest specific gene expression following VPA treatment. Hence, although VPA is one of the most potent of the HDAC inhibitors, it does not enhance the reprogramming of multipotent skin precursor cells in mice.

• The Journal of the Korean dental association
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• v.53 no.11
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• pp.817-843
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• 2015

Korean dentist's new mission was extended in dental practice field since the National Liberation Day of Korea. Modern dentistry development were due to the introduction of american dentistry, the development of Military dentistry, the unified academic activities koreans dental Association and improvement of korean dentist system. Modern dentistry development factors were as follows, First, Seoul National University's professors have been sent for studying abroad since May 1954. Thanks to this advanced system, each dental department established branch academy. Researchers and academic activities were increased in Seoul National University, Second, from January 1954, the military started training program to the korean dentists in the United States, and also the korean dentists were sent to the US Army hospital in korea for practical training courses (On the Job Training) so they could get chances to learn the advanced dentistry. During the korean war, the oral surgery dentists enlarged treatments to the maxillofacial field. Third, korean Dental academy meeting, special lectures and tables clinic from 1947 were contributing to the development of modern dentistry in the transmission of knowledge and skills. Since 1955 "Dentistry(齒學)" with a focus on the translation of foreign literature also developed modern dentistry. Since 1958 the International dental conference could broaden dental knowledge, it also provided opportunities to communicate with the world dentistry. On Oct 11, 1962, revised the Articles of association in Korean Dental academy and korean dental association unified meeting and enhanced the conferences. Improvement of korean dentist system in 1964 unified the dentists as new dentist education. Articles, case reports, review articles and academic meetings of korean dental association from 1946 to 1969 analyzed to demonstrate the development of korean modern dentistry, The titles and lectures were classified according to department and they were organized by topic.

• Environmental Analysis Health and Toxicology
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• v.24 no.2
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• pp.149-157
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• 2009

In this study, recognized building-related health symptoms of various workers in dental college hospitals are surveyed and analyzed in order to improve indoor environment at dental college hospitals. We conducted a questionnaire survey for 682 workers in three dental college hospitals located in Seoul from Sep. 20 to Oct. 8, 2004. Most workers at dental hospitals complained about noise, poor ventilation, dusty environment, and air dryness. SBS symptoms score for subjects was 1.43${\pm}$0.74 (often feel) and major symptoms was throat, CNS, eye, and nose symptoms. Recognized Building-related health symptoms are significantly related to tasks, working experience, job-satisfaction, indoor environment recognition index, medical history, used to special material, and number of dental unit chair. Also working places and ventilation are significantly related to the symptom. Based on the results, it can be said that special dental treatment causes undesirable environment such as noise, dust, and bad odor. It is also related to recognized building related health symptoms reported by workers at dental hospitals. So managemental efforts are needed to improve indoor environment which is related by dental treatment.

• Journal of Microbiology and Biotechnology
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• v.32 no.10
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• pp.1245-1252
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• 2022

Induced pluripotent stem cells (iPSCs) can be generated from somatic cells using Oct4, Sox2, Klf4, and c-Myc (OSKM). Small molecules can enhance reprogramming. Licochalcone D (LCD), a flavonoid compound present mainly in the roots of Glycyrrhiza inflata, acts on known signaling pathways involved in transcriptional activity and signal transduction, including the PGC1-α and MAPK families. In this study, we demonstrated that LCD improved reprogramming efficiency. LCD-treated iPSCs (LCD-iPSCs) expressed pluripotency-related genes Oct4, Sox2, Nanog, and Prdm14. Moreover, LCD-iPSCs differentiated into all three germ layers in vitro and formed chimeras. The mesenchymal-to-epithelial transition (MET) is critical for somatic cell reprogramming. We found that the expression levels of mesenchymal genes (Snail2 and Twist) decreased and those of epithelial genes (DSP, Cldn3, Crb3, and Ocln) dramatically increased in OR-MEF (OG2^+/+/ROSA26^+/+) cells treated with LCD for 3 days, indicating that MET effectively occurred in LCD-treated OR-MEF cells. Thus, LCD enhanced the generation of iPSCs from somatic cells by promoting MET at the early stages of reprogramming.

• Journal of Veterinary Clinics
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• v.24 no.3
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• pp.295-299
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• 2007

The detrimental effects of gene transfection on embryo development and the molecular mechanism behind the differential expression of genes related to early embryo development were assessed in the production of transgenic cow embryos through somatic cell nuclear transfer (NT). Parthenogenetic, IVF, and transgenic NT embryos derived from ${\alpha}_1$-antitrypsin transfected ear fibroblast cells was produced. To investigate the molecular mechanism behind lower developmental competence of transgenic NT embryos, the differential mRNA expression of three genes ($IFN-{\tau}$, Oct4, Fgf4) in the 3 types of embryo (Parthenogenetic, IVF, transgenic NT) was examined. RNA was extracted from ten blastocysts derived from 3 types of embryos and reverse-transcripted for synthesis of the first cDNA. The quantification of 3 gene transcripts ($IFN-{\tau}$, Oct4, and Fgf4) was carried out in three replicate by quantitative real-time reverse transcriptase PCR. Expression level of $IFN-{\tau}$ mRNA was significantly higher in transgenic NT embryos than parthenogenetic and IVF embryos (P<0.05). However, expression level of Oct4 and Fgf4 of transgenic NT embryos was significantly lower than IVF embryos (P<0.05). Altered levels of these three mRNA transcripts may explain some of the embryonic/fetal/neonatal abnormalities observed in offspring from transgenic NT embryos.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.21 no.2
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• pp.327-336
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• 1991

The purpose of this study was to evaluate the validity among the clinical, transcraial radiographic and MRI diagnosis in internal derangement of the temporomandibular joint. Fourty two temporomandibular joints were assessed in twenty one patients who visited the dental department of Chonnam National University Hospital with the complaint of temporomandibular disorder from Oct. 1990 to Oct. 1991. The results were as follows: 1. In the clinically symptomatic 29 TMJs, 26 (89.7%) joints reveal disc displacement and 3 (10.3%) joints reveal normal disc position on MRI. The sensitivity of clinical diagnosis was 0.77, and the specificity was 0.63. 2. In the normal 22 TMJs on the trans cranial radiographic finding, 16 (72.7%) joints reveal disc displacement, and 6 (27.3%) joints reveal normal disc position on MRI. In the abnormal 20 TMJs on the transcranial radiographic finding, 18 (90%) joints reveal disc displacement, and 2 (10%) joints reveal normal disc position on MRI. The sensitivity of trans cranial radio- graph was 0.53, and the specificity was 0.75.