• 제목/요약/키워드: Density gradient

검색결과 640건 처리시간 0.028초

Evaluation of normal morphology, DNA fragmentation, and hyaluronic acid binding ability of human spermatozoa after using four different commercial media for density gradient centrifugation

  • Lee, Dayong;Jee, Byung Chul
    • Clinical and Experimental Reproductive Medicine
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    • 제46권1호
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    • pp.8-13
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    • 2019
  • Objective: Density gradient centrifugation (DGC) is frequently used to isolate high-motility fractions of spermatozoa. We compared the efficacy of four DGC media in terms of the percentage of morphologically normal spermatozoa, DNA fragmentation level, and hyaluronic acid (HA) binding ability. Methods: Thirty men with a total motile spermatozoa count > 80 million participated. Semen samples were divided into four aliquots, which were processed using PureSperm, PureCeption, Sidney, and SpermGrad media, respectively. The DNA fragmentation level was measured using the Halosperm assay kit and HA binding ability was measured using the HBA assay kit. Results: The mean percentage of morphologically normal spermatozoa was significantly enhanced after DGC using all four media (10.3%, 9.9%, 9.8%, and 10.7%, respectively; p< 0.05 for each when compared with 6.9% in raw semen). The DNA fragmentation level was significantly reduced after DGC using PureSperm, PureCeption, and SpermGrad media (6.0%, 6.5%, and 4.9%, respectively; p< 0.05 for each when compared with 11.2% in raw semen), but not after DGC using Sidney media (8.5%, p> 0.05). HA binding ability did not change after DGC using any of the four media. Conclusion: The four media were equally effective for obtaining a sperm fraction with highly motile, morphologically normal sperm. PureSperm, PureCeption, and SpermGrad media were equally effective for acquiring a sperm fraction with less DNA fragmentation.

새만금 방수제 축조사면의 안정성 검토를 위한 실험적 분석 (An Experimental Analysis for the Stability Investigation of Slope on Saemangeum Lake Dykes)

  • 장동기;김기년;김동환;서관석;손문준
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2010년도 추계 학술발표회
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    • pp.687-697
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    • 2010
  • This study was designed to carry out studies on critical seepage velocity and critical hydraulic gradient using a piping test targeting SM and ML which are widely distributed ahead of and behind the depth of E.L(-)10m in Saemangeum area in order to examine stability of embankment built on the ground vulnerable to piping. The effects of relative densities on critical hydraulic gradient and critical velocity were also compared and analyzed using empirical formula and theoretical formula, and relative densities were set up as respectively 9%, 25%, 50%, and 75% for this experiment. As a result, for critical hydraulic gradient, most of specimens detected piping at lower values than the empirical formula of Terzaghi(1922). It is, therefore, considered that the empirical formula devised by Kalin(1977) or Hayashi(1978) is more reasonable to be conservative. It was also found that critical velocity decreased as relative density increased, and critical velocity predicted was mostly lower than the theoretical formula.

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소결법에 의한 $ZrO_2/Metal$계 경사기능재료에 관한 연구(1) (A Study on Zirconia/Metal.Functionally Gradient Materials by Sintering Method(1))

  • 정연길;최성철
    • 한국세라믹학회지
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    • 제31권3호
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    • pp.321-329
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    • 1994
  • Functionally gradient materials(FGM), which have the continuous or stepwise variation in a composition and microstructure, are being noticed as the material that solves problems caused by heterogeneous interface of coating or joining. And these materials also expect new functions occured by gradient composition itself. Therefore, to examine possibility of thermal barrier materials, TZP/Mo·FGM and TZP/Ni·FGM were fabricated by sintering method. As to the sintered specimens, sintering shrinkage, relative density and Vicker's hardness in each composition were examined. The phenomena due to the difference of sintering shrinkage velocity during sintering process and the thermal stress induced through differences of thermal expansion coefficient in FGM were discussed. And the structure changes at interface and microsturcture of FGM were investigated. As a results, the difference of shrinkage between ceramic and metal was about 14% in TZP/Mo and 7% in TZP/Ni. The relative density and hardness were considerably influenced by metal content changes. Owing to unbalance of sintering shrinkage velocity between ceramic and metal, various sintering defects were occured. To control these sintering defects and thermal stress, gradient composition of FGM should be narrow. The microstructure changes of FGM depended on the ceramic or metal volume percents and were analogous to the theoretical design.

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Application the mechanism-based strain gradient plasticity theory to model the hot deformation behavior of functionally graded steels

  • Salavati, Hadi;Alizadeh, Yoness;Berto, Filippo
    • Structural Engineering and Mechanics
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    • 제51권4호
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    • pp.627-641
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    • 2014
  • Functionally graded steels (FGSs) are a family of functionally graded materials (FGMs) consisting of ferrite (${\alpha}$), austenite (${\gamma}$), bainite (${\beta}$) and martensite (M) phases placed on each other in different configurations and produced via electroslag remelting (ESR). In this research, the flow stress of dual layer austenitic-martensitic functionally graded steels under hot deformation loading has been modeled considering the constitutive equations which describe the continuous effect of temperature and strain rate on the flow stress. The mechanism-based strain gradient plasticity theory is used here to determine the position of each layer considering the relationship between the hardness of the layer and the composite dislocation density profile. Then, the released energy of each layer under a specified loading condition (temperature and strain rate) is related to the dislocation density utilizing the mechanism-based strain gradient plasticity theory. The flow stress of the considered FGS is obtained by using the appropriate coefficients in the constitutive equations of each layer. Finally, the theoretical model is compared with the experimental results measured in the temperature range $1000-1200^{\circ}C$ and strain rate 0.01-1 s-1 and a sound agreement is found.

Freehand Forgery Detection Using Directional Density and Fuzzy Classifier

  • Han, Soowhan;Woo, Youngwoon
    • 한국멀티미디어학회:학술대회논문집
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    • 한국멀티미디어학회 2000년도 추계학술발표논문집
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    • pp.250-255
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    • 2000
  • This paper is concerning off-line signature verification using a density function which is obtained by convolving the signature image with twelve-directional 5$\times$5 gradient masks and the weighted fuzzy mean classifier. The twelve-directional density function based on Nevatia-Babu template gradient is related to the overall shape of a signature image and thus, utilized as a feature set. The weighted fuzzy mean classifier with the reference feature vectors extracted from only genuine signature samples is evaluated for the verification of freehand forgeries. The experimental results show that the proposed system can classify a signature whether genuine or forged with more than 98% overall accuracy even without any knowledge of vaned freehand forgeries.

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해양생태계모델에 의한 하계 진동만의 영양염변동 (Nutrient Variations in the Jindong Bay during Summer by Ecosystem Modeling)

  • 김동선;홍철훈
    • 한국해양학회지:바다
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    • 제8권2호
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    • pp.164-176
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    • 2003
  • 하계 진동만에서 얻어진 영양염 DIN(dissolved inorganic nitrogen) 및 DIP(dissolved inorganic phosphate)의 분포는 이들 농도가 만안쪽에서 매우 높은 것이 특징이다. 또 표층(0∼1 m)과 저층(8 m∼bottom)은 만 안쪽이 고농도 이고 중층(1∼8 m)은 상대적으로 낮다. 이러한 영양염의 분포특성을 바람, 조류, 밀도의 수평경도력 및 잔차류를 고려한 생태계모델을 이용하여 조사하였다. 수치실험은 이들 조건들이 영양염에 각기 어떻게 영향을 미치는 가에 주목하여 실시되었다 조석잔차류를 고려한 경우는 전반적으로 관측값보다 저농도의 분포를 보였고, 바람, 조석잔차류 및 밀도의 수평경도력에 의한 효과를 포함한 경우는 보다 관측값에 근접한 결과를 얻었다 이러한 결과는 통계적 분석 방법에서도 잘 뒤받침된다. 본 연구결과는 하계 진동만에서 영양염의 분포변동에 조류효과 뿐만이 아니라 바람에 의한 취송류 및 밀도의 수평경도력에 의해 발생하는 밀도류의 효과가 매우 중요함을 시사한다.

피압대수층을 통과하는 대심도 시추 중 적정이수밀도 결정 방법 및 적용 사례 (Determination Method of Suitable Mud Density While Drilling through Confined Aquifer and Its Application)

  • 윤운상;김유성;전형진;송윤호;이창현
    • 지질공학
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    • 제34권2호
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    • pp.217-228
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    • 2024
  • 피압대수층은 이를 통과하는 대심도 시추에서 암편 배출 불능, 지하수의 급격한 공내 유입, 이수 손실 등의 문제를 야기한다. 특히 자분 조건일 경우 과압 공극압이 발생하여 적절한 이수 밀도의 적용이 필수적이다. 이 논문에서는 이수창 분석을 이용하여 피압대수층을 통과하는 시추 중 적정이수밀도(SMD)의 결정방법을 제시하고 이를 사례에 적용하여 적용성을 검토하였다. 심도별 이수창의 하한인 최소이수밀도는 해당 심도의 등가이수밀도 공극압 구배 또는 이에 이송 마진을 더하여 산정되며, 피압대수층의 공극압 구배는 피압지하수위 또는 정두압의 측정으로 구할 수 있다. 시추공이 피압대수층에 도달하면 공극압 구배가 급격히 증가하고, 심도가 증가할 수 록 점차 감소한다. 킥 발생을 방지하기 위한 SMD는 굴진 중인 공저 심도와 그 상부 나공 구간의 최소이수밀도 중 최대값으로 결정할 수 있으며, 피압대수층 진입 이후 SMD는 피압대수층 최상부의 최소이수밀도로서 나공 구간 시추 중 이를 유지하여야 한다. 또한 적절한 케이싱의 설치는 SMD를 낮출 수 있어 고투수성 대수층에 대한 이수 침입 또는 이수 손실의 위험을 감소시킬 수 있다.

COLOR GRADIENT IN THE KING TYPE GLOBULAR CLUSTER NGC 7089

  • Sohn, Young-Jong;Chun, Mun-Suk;Lee, Jae-Woo;Oh, Jung-Min
    • Journal of Astronomy and Space Sciences
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    • 제16권2호
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    • pp.91-104
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    • 1999
  • We use BV CCD images to investigate the reality of the color gradient within a King type globular cluster NGC 7089. Surface photometry shows that there is a strong radial color gradient in the central region of the cluster in the sense of bluer center with the amplitude of ~0.39 $\pm$ 0.07 mag/$arcsec^2$ in (B - V). In the outer region of the cluster, however, the radial color gradient shows a reverse case, i.e., redder toward the center. (B - V) color profile which was derived from resolved stars in VGC 7089 field also shows a significant color gradient in the central region of the clusters, indicating that lights from the combination of red giant stars and blue horizontal branch stars cause the radial color gradient. Color gradient of the outer region of NGC 7089 may be due to the unresolved background of the cluster. Similar color gradients in the central area of clusters have been previously observed exserved exclusively in highly concentrated systems classified as post core collapse clusters. We caution, however, to confirm the reality of the color gradient from resolved stars, we need more accurate imaging data of the cluster with exceptional seeing condition because the effect of completeness correlates with local density of stars.

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증감지의 발광스펙트럼에 따른 특성곡선의 변화 (Change of Characteristic Curves using Light Emission Spectrum of the Intensifying Screens)

  • 윤철호;강홍석;이인자;허준
    • 대한방사선기술학회지:방사선기술과학
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    • 제13권2호
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    • pp.21-25
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    • 1990
  • This report is study about difference of H-D curve in regular film and ortho type film, and obtained results as follows : 1. In the blue-color emission intensifying screen, ortho type films and in the green-color emission intensifying screens, regular type are decreased in their gradient. 2. When joint use of regular type film and ortho type film, the gradient difference not seen in blue color screen. 3. The gradient difference seen clearly in G4, KM screens(green color emission intensifying). Especially, in part of high film density, the gradient difference are great. So we have to right use of screen and film because not matched used of theare the sensitivity and gradient are decrease.

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Merging Features and Optical-NIR Color Gradient of Early-type Galaxies

  • 김두호;임명신
    • 천문학회보
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    • 제36권1호
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    • pp.57.1-57.1
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    • 2011
  • It has been suggested that merging plays an important role in the formation and the evolution of early-type galaxies (ETGs). Optical-NIR color gradients of ETGs in high density environments are found to be less steep than those of ETGs in low density environments, hinting frequent merger activities in ETGs in high density environments. In order to examine if the flat color gradients are the result of dry mergers, we studied the relations between merging features, color gradient, and environments of 281 low redshift ETGs selected from Sloan Digital Sky Survey (SDSS) Stripe82. The sample contains 222 relaxed ETGs, 38 ETGs with tidal features, 10 galaxies with dust features and 11 galaxies with tidal and dust features, and Near Infrared (NIR) images are taken from UKIRT Infrared Deep Sky Survey (UKIDSS) Large Area Survey (LAS). We find that r-K color gradients of field sample galaxies are steeper than those of sample ETGs within cluster environments. For the field sample galaxies, a relatively large number of galaxies with peculiar features contribute to the steeper color gradients, while the absence of these peculiar early-type galaxies make color gradients of the cluster sample galaxies intact. In high density environment, ETGs are already evolved and relaxed, resulting flat color gradients. However, in low density environments, a majority of ETGs undergone merging recently which makes the color gradients steep.

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