• Journal of the Korean Society for Marine Environment & Energy
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• v.20 no.1
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• pp.37-44
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• 2017

Complexity of multiphase flows due to existence of more than two interface including free-surface in one system, cannot be simulated easily. Since more than two fluids affect to flows and disturb interface, non-linearities such as instabilities can be appeared. Among several instabilities on multiphase flows, one of representative is Rayleigh-taylor instability. In order to examine in importance of density disparity, several cases with numerous Atwood number are set. Moreover, investigation of influence on initial disturbance were also considered. Moving particle simulation (MPS) method, which was employed in this paper, was not widely used for multiphase problem. In this study, by adding new particle interaction models such as self-buoyance correction, surface tension, and boundary condition at interface models, MPS were developed having more strength of physics and robust. By applying newly developed multiphase MPS, considered cases are performed and compared each other. Additionally, though existence of disagreement of magnitude of rising velocity between theoretical values from linear potential theory and that of numerical simulation, agreement of tendency can be proved of similarity of result. the discordance of magnitude can be explained due to non-linear effects on numerical simulation which was not considered in theoretical result.

• Journal of Embryo Transfer
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• v.31 no.3
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• pp.169-177
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• 2016

There is a growing interest in the application of primary hepatocytes for treatment of liver diseases in humans and for drug development. Several studies have focused on long-term survival and di-differentiation blocking of primary hepatocytes in an in vitro culture system. Therefore, the present study also aimed to optimize an in vitro culture system using primary rat hepatocytes. Primary rat hepatocytes from 6-week-old male Crl:CD rats were isolated using a modified two-step collagenase perfusion. Healthy $3.5{\times}10^6$ primary rat hepatocytes were seeded into a 2 dimensional (2D) culture in a 25T culture flask coated with collagen type I or into a 3D culture in a 125-ml spinner flask for 7 days. Production of plasma protein (ALB and TF), apoptosis (BAX and BCL2), and CYP (CYP3A1) related genes were compared between the 2D and 3D culture systems. The 3D culture system had an advantage over the 2D system because of the relatively high expression of ALB and low expression of BAX in the 3D system. However, the level of CYP3A1 did not improve in the 3D culture with and without the presence of a dexamethasone inducer. Therefore, 3D culture has an advantage for albumin production and primary rat hepatocyte survivability, but a low expression of CYP3A1 indicated that primary rat hepatocytes require a high-density culture for stress reduction by continuous flow.

• Applied Chemistry for Engineering
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• v.10 no.5
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• pp.652-659
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• 1999

The trend of poisoning of reforming catalyst along with the position of anodic catalyst bed was studied. Keeping the conditions that steam to carbon ratio was 2.5, operating voltage was 0.75 V, current density was $140mA/cm^2$, the unit cell was operated during 24 hrs at a steady state. And then the cell was stopped, the catalysts packed in the position of inlet, middle and outlet were sampled individually and then the amount of carbon, Li and K poisoned were analysed. After 100 hrs operated, the catalysts at the same positions were analysed at the same manner. The result of this experiment was as followings. After 24 hrs operated, the poisoning amounts of Li and K in the catalyst were 0.27 wt% at inlet, 0.23 wt% at middle and the highest value 1.59 wt% at outlet. After 100 hrs, the amount of poisoning is the highest in the catalyst packed at the inlet of unit cell. The performance simulation of unit cell explained these trends of poisoning catalysts. The simulation told that the catalyst in the region of the inlet of unit cell treated the 90% of initial methane flow rate and the highest electrochemical reaction happened in this region. So the catalysts of this region were the most poisoned with carbon, Li and K and also the rate of poisoning is faster than that of the catalyst at other regions. The temperature at the region of outlet of unit cell was $30^{\circ}C$ higher than that of other regions, so more Li, and K vaporized than at other regions and little reforming reaction at this region made the catalysts poisoning rate low.

• Journal of Korean Society of Transportation
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• v.17 no.4
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• pp.59-69
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• 1999

A Freeway corridor is a network consisting of a few Primary longitudinal roadways (freeway or major arterial) carrying a major traffic movement with interconnecting roads which offer the motorist alternative paths to his/her destination. Control measures introduced to ameliorate traffic performance in freeway corridors typically include ramp metering at the freeway entrances, and signal control at each intersections. During a severe freeway incident, on-ramp metering usually is not adequate to relieve congestion effectively. Diverting some traffic to the Parallel surface street to make full use of available corridor capacity will be necessary. This is the purpose of the traffic management system. So, an integrated traffic control scheme should include three elements. (a)on-ramp metering, (b)off-ramp diversion and (c)signal timing at surface street intersections. The purpose of this study is to develop an integrated optimal control model in a freeway corridor. By approximating the flow-density relation with a two-segment linear function. the nonlinear optimal control problem can be simplified into a set of Piecewise linear programming models. The formulated optimal-control Problem can be solved in real time using common linear program. In this study, program MPL(ver 4.0) is used to solve the formulated optimal-control problem. Simulation results with TSIS(ver 4.01) for a sample network have demonstrated the merits of the Proposed model and a1gorithm.

• Imaging Science in Dentistry
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• v.33 no.3
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• pp.143-149
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• 2003

Purpose: To investigate the radiosensitivity of the normal human oral keratinocytes (NHOK), and the effect of irradiation on cell cycle and protein expression. Materials and Methods: To evaluate the radiosensitivity of NHOK, the number of colonies and cells were counted after irradiation and the SF2 (survival fraction at 2Gy) value, and the cell survival curve fitted on a linear-quadratic model were obtained. LDH analysis was carried out to evaluate the necrosis of NHOK at 1, 2, 3, and 4 days after 2, 10, and 20 Gy irradiation. Cell cycle arrest and the induction of apoptosis were analyzed using flow cytometry at 1, 2, 3, and 4 days after 2, 10, and 200y irradiation. Finally, proteins related cell cycle arrest and apoptosis were analysed by Western blot. Results: The number of survived cell was significantly decreased in a dose-dependent manner. The cell survival curve showed SF2, α, and β values to be 0.568, 0.209, and 0.020 respectively. At 200y irradiated cells showed higher optical density than the control group. After irradiation, apoptosis was not observed but G2 arrest was observed in the NHOK cells. 1 day after 10 Gy irradiation, the expression of p53 remained unchanged, the p2l/sup WAF1/Cipl/ increased and the mdm2 decreased. The expression of bax, bcl-2, cyclin B1, and cyclin D remained unchanged. Conclusion: These results indicate that NHOK responds to irradiation by G2 arrest, which is possibly mediated by the expression of p21/sup WAFl/Cipl/, and that cell necrosis occurs by high dose irradiation.

• Journal of Hydrogen and New Energy
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• v.10 no.1
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• pp.59-69
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• 1999

AlN thin films were fabricated by reactive sputtering for the application of MIS devices with Al/AlN/Si structure. It has investigated the surface morphology change, I-V characteristics, C-V characteristics, and chemical composition of AlN films with the intriducing time of hydrogen on the fixed deposition condition(RF power: 150W, sputtering pressure: 5mTorr, flow rate ratio of $Ar/N_2=1$, hydrogen concentration: 5%). By addition of the hydrogen the deposition rate decreased drastically whereas the surface morphology changed little. It has been found from the analysis of I-V and C-V characteristics curves that the films deposited with hydrogen addition in initial stage had lower leakage current density, lower flat band voltage and hystersis profile when compared with those with hydrogen addition in last stage. The oxygen concentration in AlN films decreased with addition of hydrogen gas, which suggesting a profitable role in the insulation and C-V characteristics of AlN films.

• Fire Science and Engineering
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• v.16 no.1
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• pp.1-7
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• 2002

Halogen-based fire suppressing agents have environmental problems because they cause the stratospheric ozone depletion and globe warming. Hence, fire suppression system using fine water mist became the center of interest as a substitution of halon. As a study about this, it is in progress to make the optimum droplet size by using water mist nozzles and to improve the extinguishing performance of water mist by using additives. Before this study, the extinguishing time of ethanol and n-heptane pool fire was measured with changing of water mist droplet size, flow density, discharge pressure, and fire size. In this study, on adding the additives to improve physical and chemical extinguishing performance of water mist, the extinguishing performance would evaluate and the optimum condition would find out. As a result, in case of ethanol pan 1 pool fire, the extinguishing time of the water mist by adding of 2.5 wt% NaCl and 0.3% AFFF got shorter 27% and 60% than the pure water mist. Adding of AFFF was to decrease the flame temperature by forming thin film on the fuel surface and to decrease the evaporation of n-heptane fuel. In case of NaCl, alkali salt crystals showed on the flame surface.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.3
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• pp.327-339
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• 2003

During the winter in February 1998, January and April 1999, interdisciplinary research was conducted in a large area including the South Sea of Korea and northern East China Sea to examine distribution and structure. Water masses identified from the observed data are Warm Water originated from Tsushima Warm Current, Yellow Sea Cold Water (Northern or Central Cold Water) and Korean Southern Sea Cold Water. In the southern Yellow Sea, Warm Water originated from Tsushima Warm Current, flowing into the Cheju Strait after turning around the western Cheju Island, makes a front of '┍' shape, which is bounded by the Yellow Sea Central Cold Water in the southern part of Daeheuksan Island and by the Yellow Sea Northern Cold Water in the eastern part of the Yangtze Bank. This front changes its corner shape and position with strength of the warm water extension toward northwestern Yellow Sea. The position and structure of the fronts off the southwestern tip of the Korean peninsular and near the Yangtze Bank varies with observation period. In the front in the South Sea of Korea, cold coastal water which if formed independently due to local cooling, ,sinks along the sloping bottom. We explained the processes of variations in the distribution and structure of these winter fronts in terms of up-wind and down-wind flow by the seasonal monsoon, heat budget through the sea surface and density difference across the fronts.

• Journal of Chest Surgery
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• v.38 no.10 s.255
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• pp.725-728
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• 2005

A case is described in a girl who presented with recurrent life-threatening hemoptysis at the age of 18 months, and had been diagnosed as atrial septal defect with severe cardiomegaly which was presumed to result in pulmonary vein stenosis at the age of 6 months. Closure of atrial septal defect was associated with decreased heart size and improved pulmonary venous flow. However, recurrent life-threatening hemoptysis occurred during follow-up, Computed tomography scan demonstrated left pulmonary vein stenosis and extrinsic compression of the left bronchus by multiple soft tissue density-masses. Exploratory thoracotomy revealed single stenotic left pulmonary vein, and flat left main bronchus compressed by multiple hypertrophied lymph nodes, Unexpected endotrachial tube bleeding during left hilar dissection mandated to proceed to left pneumonectomy, The patient's postoperative course was uneventful. Follow-up chest roentgenography revealed acceptable left hydrothorax without mediastinal shifting, Nevertheless, a long-term follow-up is necessary.

• Proceedings of the Korea Water Resources Association Conference
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• 2009.05a
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• pp.880-884
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• 2009

여름철 강우시 저수지로 유입하는 고탁도의 하천수는 저수지 주변 수체에 비해 낮은 수온과 높은 부유물질 농도(SS)로 인해 상대적으로 높은 밀도를 가지며, 저수지 내에서 동일 밀도층을 따라 분포하며 밀도류를 형성하게 된다. 탁수는 대부분 장기간 저수지에 체류하며 수질과 수생태계에 큰 영향을 주게 된다. 따라서 저수지로 유입한 탁수의 밀도류 거동특성을 파악하는 것은 저수지 운영, 수질관리 및 수생태계 보호를 위한 관리대책 수립에 있어서 중요한 요소이다. 본 연구의 목적은 횡방향 평균 2차원 수치모델을 이용하여 초기수위별 다양한 유량규모에서 소양호로 유입하는 밀도류의 거동특성(유입경계지점 수심($h_0$), 침강점 수심($d_p$)과 거리($X_p$), 분리점 수심($d_s$), 중층류 관입두께($h_i$), 댐 축 도달시간($t_a$), 희석율(${\beta}$))을 분석하여 저수지 수질관리를 위한 의사결정지원 기초정보를 제공하는데 있다. 밀도류의 거동분석을 위해 사용된 유량조건은 그동안 소양호에서 발생한 홍수크기를 바탕으로 7개의 등급으로 나누었으며, 각각의 유량조건별 수문곡선은 2007년 수문사상 중 7월 30일부터 8월 30일까지의 수문사상을 바탕으로 수정가우시안 공식을 사용하여 산정하였다. 탁수 거동 특성의 모의는 서로 다른 초기 수위 및 유량조건하에서의 탁수거동을 나타냈고, SS농도 25 mg/L 이상을 기준으로 하여 탁수층의 $d_p$, $X_p$, $d_s$, $h_i$, $t_a$, ${\beta}$를 산정하였다. 일반적으로 유량규모가 커질수록 $d_p$와 $d_s$는 증가하였고, $X_p$는 댐 축으로부터 가까워짐이 확인되었으나, 동일 유량규모에 대해 초기 수위가 증가함에 따라 $X_p$는 대체로 댐으로부터 멀어졌다. $h_i$는 유량규모가 증가함에 따라 완만하게 증가하는 경향을 보였고, $t_a$는 초기수위가 EL. 165 m일 때 유량이 2,000 CMS 미만인 경우 댐 앞까지 도달하지 못하는 것으로 나타났으며, 나머지 수위조건에서는 유량이 3,000 CMS 미만인 경우 댐 앞까지 도달하지 못하는 것으로 나타났다. 유량 규모에 따라 $X_p$가 0 km인 지점과 19 km인 지점에서의 ${\beta}$ 값을 산정한 결과 일반적으로 유량규모가 커질수록 유입수의 희석효과는 작아지며 초기수위가 커질수록 증가하는 경향을 나타냈다. 연구 결과는 탁수 발생 초기 저수지 운영 실무자들이 유량규모 및 초기 수위조건에 따른 밀도류의 거동특성을 신속히 예측하는 목적으로 사용될 수 있다.