• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.59-63
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• 1999

Efficient plant regeneration via shoot organogenesis from in vitro cultured leaf segments of chrysanthemum (Dendranthema grandiflora Tzvelev cv. Namjeon) was achieved. Adventitious shoot formation from leaf explants was greatly influenced by plant growth regulator, leaf age, light condition, explant number per culture vessel, and explant orientation. Leaf segments, obtained from fully expanded young 1-2nd leaves and inoculated 8 explants per petri-dish with adaxial surface contact with MS medium supplemented with 0.5 mg/L BA and 2.0 mg/L NAA, produced 100% regeneration frequency and 13.7 shoots per explant. Regenerated adventitious shoots were successfully rooted in MS medium with 0.1 mg/L NAA. The plantlets were acclimatized in artificial soil mixtures (Vermiculite:Perlite=1:1), and transferred to greenhouse for flowering. The regenerated plants showed normal phenotypes.

• Horticultural Science & Technology
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• v.30 no.3
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• pp.325-328
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• 2012

A new Dendranthema grandiflourm 'My Sun' was developed at Gyeonggi-do Agricultural Research & Extension Services (GARES), Korea in 2009. 'My Sun' was initially derived from the cross in 2005 between 'Omega Time Orange', a potted chrysanthemum cultivar with orange single type, and 'Tasman', a potted chrysanthemum cultivar with white single type in 2005. The cultivar has single type flowers with yellow petals. Trial and evaluation was conducted from 2006 to 2009 for the selection of this variety, including a shading culture in spring and a retarding culture in winter. The flowering time of 'My Sun' was October 13th, and year-round flowering is possible by shading or lighting treatment. The diameter of flower is 21.0 mm. Numbers of flowers per stem and petals per flower are 34.4 and 20.4, respectively. Its leaf color was green (Green Group 137A) and plant height was 13.3 cm. Days to flowering under the short day treatment is about 42 in spring, and numbers of branch per plant was 3.4 ea in the winter. This cultivar was resistance to white rust and consumer's preference of new pot-mum is high level than control.

• Journal of Plant Biotechnology
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• v.36 no.4
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• pp.373-383
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• 2009

To set efficient transformation system in chrysanthemum, thirty-four chrysanthemum (Dendranthema grandiflorum Kitamura) varieties were collected and cultured for shoot regeneration. Five varieties, ‘Shuho-no-chikara', ‘Zinba', ‘Baekma', ‘Pink pride' and ‘Keumsu' of them were selected, because they had a high shoot regeneration efficiency. MS medium containing 1.0 mg/L NAA and BA respectively was very adequate for shoot regeneration in those varieties. MS medium with 3.0 mg/L NAA and 1.0 mg/L kinetin in ‘Shuho-no-chikara' and the medium with 0.5 mg/L NAA and 3.0 mg/L BA in ‘Keumsu' were also suitable for shoot regeneration. The most efficient callus induction and shoot regeneration were obtained on MS medium. Shoot regeneration was enhanced more than 8% on MS medium with 0.3% phytagel and 10-15 mg/L putrescine. The best cultural material for shoot regeneration was stem. When stem was used as a culture material, shoot regeneration rate was increased more than 26% and the days to shoot regeneration was shortened about 14 days.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.335-339
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• 2003

Several experiments were carried out to transfer LEAFY gene to Dendranthema grandiflora 'Shuho-no-chikara' by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene. Kanamycin 10mg/L was used in first selection medium, and 20mg/L in the second one. Co-culture for 3 days was more effective in increasing transformation efficiency than that for 7 days. The transformation efficiency by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene was about 2.8％ until the second selection, but only 0.13％ of shoots (two plants) was confirmed as a transgenic plants in Southern analysis. The escape of putative transformants was occured seriously in the process of selections, PCR analysis for confirming of neomycin phosphotransferaseII (npt II), and Southern analysis for LEAFY gene. One transgenic plant appeared 7 days'early flowering in field.

• Korean Journal of Medicinal Crop Science
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• v.23 no.3
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• pp.214-222
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• 2015

This study was conducted to evaluate the growth conditions and vegetation of Peucedanum japonicum habitat in uninhabited islands of Incheon Korea. The emergence area of Peucedanum japonicum was 4.9 m distance in coastline, and altitude and slope was 7.4m and 47.5%. Soil pH, organic matter and $P_2O_5$ of habitat were 7.1, 8.3% and 29.0 mg/kg, and contents of K, Ca, Mg and Na were 0.66, 4.9, 3.4 and $1.13cmol^+/kg$, respectively. Growth plants with Peucedanum japonicum were total 55 taxa, and Gakeuldo in uninhabited islands was the most as 25 taxa. In growth plants with Peucedanum japonicum, plants of over 25% frequence per plot were Miscanthus sinensis, Dendranthema boreale, Lilium lancifolium, Hemerocallis middendorffii, Elymus dahuricus, Cirsium japonicum var. maackii, Asparagus schoberioides, Gypsophila oldhamiana, Cocculus trilobus, Silene aprica var. oldhamiana, Artemisia capillaris, Crepidiastrum denticulatum and Sedum oryzifolium. The vegetation of Peucedanum japonicum habitat was classified into Aster spathulifolius community, Miscanthus sinensis community, Dendranthema boreale community, Setaria viridis var. pachystachys community, Cirsium japonicum var. maackii community and Hemerocallis middendorffii community. According to general condition of habitat, suitable growing areas was determined to slightly acidic from mild alkaline soils.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.295-298
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• 2010

A new spray chrysanthemum(Dendranthema grandiflorum) cultivar, 'Biz' was developed from a cross between 'S03-117' and 'Angaesoguk' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services(ARES) from 2003 to 2008. Its characteristics were investigated three times from 2006 to 2008 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Biz' was October 26th, and year-round production was possible by day length treatment. This cultivar was white in color, single in flower type for the spray type cut flower. Its capitulum was 3.0 cm in diameter, and had 15.6 head per stem in autumn. Its ray floret was green central zone. The days to flowering under the short day treatment is about 42 days in spring season, and 'Biz' showed the vase life of 24.1 days in autumn. This cultivar was registered for a commercialization in 2008.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.606-610
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• 2010

A new cultivars Dendranthema grandiflourm 'Dream Moon' was developed at Gyeonggi-do Agricultural Research and Extension Services (GARES), Korea in 2008. The cultivar 'Dream Moon' was derived from a cross between 'Patra', a spray cultivar with yellow single type, and 'Sei-rosa', a spray cultivar with pink single type in 2005. The cultivar has single type flowers with pink petals. Trial evaluation was conducted from 2006 to 2008 for a shading cultivation in summer and a retarding cultivation in spring. The flowering time of 'Dream Moon' is late October, and year-round flowering is possible by shade or light treatment. The diameter of flower is 56.0 mm. Numbers of flowers per stem and petals per flower are 16.4 and 24.6, respectively. After investing of specific characters from 2006 to 2008, it was finally selected and named 'Dream Moon'. It has resistance to white rust and the vase life is about 20.7days in autumn season.

• Journal of Environmental Science International
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• v.30 no.3
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• pp.235-243
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• 2021

This study was conducted to investigate the growth of Dendranthema zawadskii in damaged soils when they are treated with improvement agents. The treatments consisted of a control (unamended field soil) and the application of a loess ball of 1 cm to the field soil. According to the degree of damage the de-icing agent had caused, the soils were divided into 3 areas (based on the yellowing of Pinus densiflora for. multicaulis in soil surveys): H (high saline), M (medium saline), and L (low saline). A total of six treatments were performed: D. zawadskiia plant without soil amendment (H; high saline soil, M; medium saline soil, L; low saline soil), and a D. zawadskiia plant with loess ball on the soil surface (H.L; high saline soil with loess ball, M.L; medium saline soil with loess ball, L.L; low saline soil with loess ball). The results showed that D. zawadskiia growth went from highest to lowest in the order: M.L > L.L > M > L > H.L > H. Plant growth results showed that soils treated with soil amendments (loess ball) were better for D. zawadskii growth than untreated soils.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.109-109
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• 2020

꽃차(Flower tea)는 최근 다양한 제품이 개발되고 소비가 확대되는 등 그 산업적 가치가 증대하고 있다. 꽃차의 향기 특성은 우렸을 때 나타나는 색깔 및 인체에서의 생리활성과 더불어 주요한 꽃차 품질결정 요소이다. 본 연구는 꽃차로의 이용이 활발한 국화과 식물 중 구절초(Dendranthema zawadskii var. latilobum), 국화(Chrysanthemum morifolium), 노랑색 및 주황색 마리골드(Tagetes erecta 'Yellow' and 'Orange'), 그리고 캐모마일(Matricaria chamomilla)의 향기 성분특성을 구명하기 위하여 제조된 꽃차를 headspace-solidphase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS)를 이용하여 분리, 동정하였다. 국화과 꽃차로부터 총 117종의 휘발성 성분이 확인되었는데, 각 꽃차 종류별 동정된 휘발성 성분의 개수, 주요 3개 휘발성 성분과 전체 향기성분 peak중 이들이 차지하는 구성비율(%)은 다음과 같았다: 구절초 64종, camphor (31%), α-pinene(14%), camphene(14%); 국화 60종, camphor(15%), chrysantheny acetate(13%), eucalyptol (11%); 마리골드 '옐로우' 53종, 2,4-heptadienal(26%), trans-isocarveol(21%), cis-β-Copaene(18%); 마리골드 '오렌지' 61종, β-caryophyllene(16%), β-ocimene epoxide(12%), β-ocimene(12%); 캐모마일 50종, β-farnesene(63%), nonane(9%), spathulenol(5%). 국화과 꽃차 5종 모두에서 공통적으로 검출된 성분은 β-caryophyllene, α-pinene, β-farnesene 등 10종이었으며 마리골리 '옐로'는 '오렌지'와 주요 향기성분의 조성에서 뚜렷한 차이를 나타내었다. 비록 그 함량은 낮았으나 구절초, 국화, 마리골드 '오렌지', 그리고 캐모마일은 각각 10종, 12종, 3종 및 13종이었다. 마리골드 '엘로'의 경우 검출된 모든 향기성분은 마리골드 '오렌지'나 다른 국화과 식물의 꽃차에서도 검출된 바, 향기 성분이 다양성이 다소 낮게 나타났다.

• The Plant Pathology Journal
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• v.22 no.3
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• pp.230-234
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• 2006

Tomato spotted wilt virus (TSWV) has been identified in commercial chrysanthemum cultivars in Korea. Nucleotide sequences of the N gene of TSWV-ch14 isolated from infected chrysanthemum were determined and deposited in GenBank under accession no. DQ453158. The symptoms consisted of dark colored leaf necrosis, black streaks along the stem, wilting of plant parts in 'Sinma'; and chlorotic spots, necrosis of axillary shoots and withering of leaves in 'Hwarang'. Electron micrographs of leaf preparation of Nicotiana rustica infected with TSWV-ch14 contained spherical particles around 85 nm in diameter. TSWV was identified from chrysanthemum by sequence determination of N nucleocapsid protein and virion observation by transmission electron microscope. This is the first reported observation on TSWV in chrysanthemum in Korea.