• Journal of Digital Convergence
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• v.11 no.8
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• pp.11-21
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• 2013

Integrated Social Welfare Management System is an information system that comprehensively manages information and services regarding diverse welfare benefits provided by the government. It is designed to improve efficiency of social welfare administration and the service delivery system. The system is found to have significantly contributed to efficiency of the welfare delivery system by reducing the workload of public officials who are responsible for welfare administration in local governments and changing the way they work for the better. This study analyzes the costs and benefits of the Integrated Social Welfare Management System and describes what it has achieved for the provider and recipients. The system has enhanced efficiency of the welfare delivery system and enabled case management, the function social welfare public officials are supposed to do. Ultimately, the system will play a key role in building a consumer-centered welfare administration environment in the future.

• Journal of Pharmaceutical Investigation
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• v.34 no.4
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• pp.263-267
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• 2004

Branched and linear polyethylenimines (PEIs) have been studied as efficient and versatile agents for gene delivery in vitro and in vivo. PEIs exist in a linear or branched topology and are available in a wide range of molecular weight (Mw). Most studies have been done using PEIs with Mw higher than 10Kd. This study was aimed to test the transfection efficiency and the cell viability following gene delivery using PEI of Mw 2Kd, a relatively lower Mw cationic polymer. We used murine interleukin-2(mIL-2) plasmid DNA complexed with branched PEI 2Kd or 25Kd, and transfected them into a myoblast muscle cell line, C2C12. The cellular uptake of mIL-2 plasmid DNA was determined using quantitative polymerase chain reaction. RNA transcript levels were studied in the myoblast cells. Our results show that PEI 2Kd was as effective as PEI 25Kd in celluar gene delivery and transfection efficiency in C2C12 cells. Moreover, MTT assay indicated that PEI 2Kd/DNA complexes did not significantly reduce the cell viability regardless of N/P ratios. These results suggest that PEI of Mw 2Kd might play a role as effective and low toxic nonviral vector systems for muscular cell lines.

• Journal of Advanced Marine Engineering and Technology
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• v.40 no.9
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• pp.756-761
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• 2016

The objective of this study is to design and manufacture a fuel pump with the plunger diameter of 4 mm and stroke of 7 mm that can be mounted in a small single-cylinder four-stroke agricultural diesel engine, and to investigate the performance characteristics of the pump. The combustion pressure in a cylinder was reproduced by forming the back pressure of 1, 6, 11, 16 and 21 bar with a nitrogen gas in the home-manufactured modeling cylinder. In the experiment, the discharge pressure was measured at the spot of 1 cm away from the discharge port of a developed fuel pump. The delivery pressure and delivery flow rate were measured at the spot of 30 cm away from the discharge port of the pump, and the pump efficiency was calculated. The pump motor speed was changed from 600 to 800, 1000, 1200 and 1400 rpm. It is found that the delivery feed rate of fuel pump is increased as the rotational speed is raised, and is decreased as the back pressure, compression pressure in the cylinder, is increased. Also, the pump efficiency is reduced as the rotational speed and back pressure are increased.

• International Journal of Oral Biology
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• v.39 no.4
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• pp.177-185
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• 2014

Transfection is a gene delivery tool that is a popular means of manipulating cellular properties, such as induced pluripotent stem cell (iPSC) generation by reprogramming factors (Yamanaka factors). However, the efficiency of transfection needs to be improved. In the present study, three transfection protocols - non-liposomal transfection (NLT), magnetofection and electroporation - were compared by analysis of their transfection efficiencies and cell viabilities using human dental pulp cells (hDPC) and bovine fetal fibroblasts (bFF) as cell sources. Enhanced green fluorescent protein gene was used as the delivery indicator. For magnetofection, Polymag reagent was administrated. NLT, FuGENE-HD and X-treme GENE 9 DNA transfection reagents were used for NLT. For electroporation, the $Neon^{TM}$ and $NEPA21^{TM}$ electroporators were tested. $Neon^{TM}$ electroporation showed highest transfection efficiency when compared with NLT, magnetofection, and $NEPA21^{TM}$ electroporation, with transfection efficiency of about 33% in hDPC and 50% in bFF, based on viable cell population in each cell type. These results suggest that transfection by $Neon^{TM}$ electroporation can be used to deliver foreign genes efficiently in human and bovine somatic cells.

• Macromolecular Research
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• v.14 no.3
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• pp.348-353
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• 2006

For enhancing the gene delivery efficiency of polyplexes, a new formulation was developed using PEI conjugated Pluronic F127 copolymer as an effective additive. Low molecular weight, branched polyethylenimine Mw 600 (LMW BPEI 600) was conjugated to the terminal end of Pluronic F127. The PEI-modified Pluronic copolymers formed a micellar structure in aqueous solution, similar to that of unmodified Pluronic copolymer. PEI modification of Pluronic copolymer increased the size of micelles while concomitantly raising the critical micelle concentration (CMC). The PEI-modified Pluronic copolymer was used as a micellar additive to enhance the gene transfection efficiency of pre-formulated polyelectrolyte complex nanoparticles composed of luciferase plasmid DNA and branched PEI Mw 25k (BPEI 25k) or polylysine Mw 39k (PLL 39k). The luciferase gene expression levels were significantly enhanced by the addition of the BPEI-modified Pluronic copolymer for the two formulations of BPEl and PLL polyplexes. The results indicated that the BPEI-modified Pluronic copolymer micelles ionically interacted on the surface of DNA/BPEI (PLL) polyplexes which might facilitate cellular uptake process.

• Bulletin of the Korean Chemical Society
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• v.22 no.1
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• pp.46-52
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• 2001

To evaluate the non-ionic polymer, poly(ethylene glycol) (PEG), as a component in cationic copolymers for non-viral gene delivery systems, PEG was coupled to polyethylenimine (PEI). We present the effects of different degrees and shapes of pegylation of PEI on cytotoxicity, water solubility and transfection efficiency. This work reports the synthesis and characterization of a series of cationic copolymers on the basis of the conjugates of PEI with PEG. The modified molecules were significantly less toxic than the original polymer. Moreover, the chemical modification led to enhancement of their solubility. The comparison of pegylated PEIs with different degrees of derivation showed that all the polymers tested reached comparable levels of transgene expression to that of native PEI. As assessed by agarose gel electrophoresis, even highly substituted PEI derivatives were still able to form polyionic complexes with DNA. However, aside from an increase in solubility and retention of the ability to condense DNA, methoxy-PEG-modified PEIs resulted in a significant decrease in the transfection activity of the DNA complexes. In fact, the efficiency of the copolymer was compromised even at a low degree of modification suggesting that the PEG action resulting from its shape is important for efficient gene transfer. The mode of PEG grafting and the degree of modification influenced the transfection efficiency of PEI.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.1456-1461
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• 2004

Companies of Build-to-Order(BTO) strive to achieve customer responsiveness and cost efficiency simultaneously. The success of BTO depends upon the high volume production based on product plat form and delayed differentiation principle on the one hand, and upon the rapid estimation of delivery and cost for the customer orders on the other hand. Expeditious processing of a specific order requires the rearrangement of production resources and the schedule, which results in increased cost. This paper describes a cost estimation method using activity-based costing depending on the schedule change caused by urgent customer orders.

• Journal of the Korean Operations Research and Management Science Society
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• v.28 no.1
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• pp.1-10
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• 2003

A recent trend In flexible manufacturing systems (FMS) Is to utilize the Identical versatile machines with fast tool delivery devices. Unlike a conventional FMS where parts are fed to the machines with the required tools, tools are acquired from other machines using a tool delivery system and parts can be finished on the same machine In these FMS. Therefore it Is more important problem 4o plan tool flow using tool delivery system In these FMS rather than part flow In conventional FMS. According to the existing study, In FMS 20∼30 percent of the total management cost Is the cost related with tools and It Is possible to reduce the tool Inventory by 40 percent using efficient tool allocation. In this study, In FMS under dynamic tool allocation strategy, a new method of part release considering tool flow at the stage of part release Is proposed. In order to prove the efficiency of the proposed part release It is compared with other part release through simulation experiments. Performance measures in these experiments are the throughput and the number of tardy parts.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.788-794
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• 2015

Cationic liposomes have been actively used as gene delivery vehicles despite their unsatisfactory efficiencies because of their relatively low toxicity. In this study, we designed novel heterodimeric peptides as nonviral gene delivery systems from TAT and NLS peptides using cysteine-to-cysteine disulfide bonds between the two. Mixing these heterodimeric peptides with DNA before mixing with lipofectamine resulted in higher transfection efficiencies in MCF-7 breast cancer cells than mixing unmodified TAT, NLS, and a simple mixture of TAT and NLS with DNA, but did not show an adverse effect on cell viability. In gel retardation assays, the DNA binding affinities of heterodimeric peptides were stronger than NLS but weaker than TAT. However, this enhancement was only observed when heterodimeric peptides were premixed with DNA before being mixed with lipofectamine. The described novel transfection-enhancing peptide system produced by the heterodimerization of TAT and NLS peptides followed by simple mixing with DNA, increased the gene transfer efficiency of cationic lipids without enhancing cytotoxicity.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.26 no.2
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• pp.68-74
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• 2003

Modem automated manufacturing processes employ automated guided vehicles(AGVs) for material handling, which serve several machining centers(MCs) in a factory. Optimal scheduling of AGVs can significantly help to increase the efficiency of the manufacturing process by minimizing the idle time of MCs waiting for the raw materials. In this paper, we will analyse the requirements for an optimal schedule and then provide a mathematical framework for an efficient schedule of material delivery by an AGV. With this model, the optimal number of MCs to be utilized will also be determined. Finally, the material delivery schedule employing multiple journeys to the MCs by the AGV will be carried out. Through rigorous analysis and simulation experiments, we shall show that such a delivery strategy will optimize the overall performance.