• Molecules and Cells
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• v.42 no.11
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• pp.783-793
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• 2019

When endoplasmic reticulum (ER) functions are perturbed, the ER induces several signaling pathways called unfolded protein response to reestablish ER homeostasis through three ER transmembrane proteins: inositol-requiring enzyme 1 (IRE1), PKR-like ER kinase (PERK), and activating transcription factor 6 (ATF6). Although it is important to measure the activity of ATF6 that can indicate the status of the ER, no specific cell-based reporter assay is currently available. Here, we report a new cell-based method for monitoring ER stress based on the cleavage of $ATF6{\alpha}$ by sequential actions of proteases at the Golgi apparatus during ER stress. A new expressing vector was constructed by using fusion gene of GAL4 DNA binding domain (GAL4DBD) and activation domain derived from herpes simplex virus VP16 protein (VP16AD) followed by a human $ATF6{\alpha}$ N-terminal deletion variant. During ER stress, the GAL4DBD-VP16AD(GV)-$hATF6{\alpha}$ deletion variant was cleaved to liberate active transcription activator encompassing GV-$hATF6{\alpha}$ fragment which could translocate into the nucleus. The translocated GV-$hATF6{\alpha}$ fragment strongly induced the expression of firefly luciferase in HeLa Luciferase Reporter cell line containing a stably integrated 5X GAL4 site-luciferase gene. The established double stable reporter cell line HLR-GV-$hATF6{\alpha}$(333) represents an innovative tool to investigate regulated intramembrane proteolysis of $ATF6{\alpha}$. It can substitute active pATF6(N) binding motif-based reporter cell lines.

• Journal of Information Technology Services
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• v.13 no.4
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• pp.359-375
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• 2014

Researchers cannot avoid missing data in collecting data, because some respondents arbitrarily or non-arbitrarily do not answer questions in studies and experiments. Missing data not only increase and distort standard deviations, but also impair the convenience of estimating parameters and the reliability of research results. Despite widespread use of hot deck, researchers have not been interested in it, since it handles missing data in ambiguous ways. Hot deck can be complemented using K-nn, a method of machine learning, which can organize donor groups closest to properties of missing data. Interested in the role of k-nn, this study was conducted to impute missing data based on the hot deck method using k-nn. After setting up imputation of missing data based on hot deck using k-nn as a study objective, deletion of listwise, mean, mode, linear regression, and svm imputation were compared and verified regarding nominal and ratio data types and then, data closest to original values were obtained reasonably. Simulations using different neighboring numbers and the distance measuring method were carried out and better performance of k-nn was accomplished. In this study, imputation of hot deck was re-discovered which has failed to attract the attention of researchers. As a result, this study shall be able to help select non-parametric methods which are less likely to be affected by the structure of missing data and its causes.

• Journal of Korea Multimedia Society
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• v.24 no.12
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• pp.1641-1652
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• 2021

To analyze the crime scene, the role of digital evidence such as CCTV and black box is very important. Such digital evidence is often damaged due to device defects or intentional deletion. In this case, the deleted video can be restored by well-known techniques like the frame-based recovery method. Especially, the data such as the video can be generally fragmented and saved in the case of the memory used almost fully. If the fragmented video were recovered in units of images, the sequence of the recovered images may not be continuous. In this paper, we proposed a new video restoration method to match the sequence of recovered images. First, the images are recovered through a frame-based recovery technique. Then, after analyzing the time information marked on the images, the time information was extracted and recognized via optical character recognition (OCR). Finally, the recovered images are rearranged based on the time information obtained by OCR. For performance evaluation, we evaluate the recovery rate of our proposed video restoration method. As a result, it was shown that the recovery rate for the fragmented video was recovered from a minimum of about 47% to a maximum of 98%.

• Journal of KIISE:Databases
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• v.31 no.1
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• pp.48-60
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• 2004

XML documents consist of elements that are basic units of information. When the structure of XML documents is changed dynamically, we need to update structure information efficiently without changing the information of the index structure for fast retrieval. In this paper, we propose a dynamic indexing model scheme that updates the index structure in real time as the structure of XML documents is changed by insertion and deletion of elements. Our dynamic indexing model consists of a structure information representation method and a dynamic index structure. The structure information representation method supports various types of structure retrievals. Our dynamic index structure processes various structural queries efficiently. We show through various experiments that our method outperforms existing ones in processing various types of queries such as content based queries, structural queries and hybrid queries.

• The KIPS Transactions:PartC
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• v.12C no.1 s.97
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• pp.53-62
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• 2005

The NG-SDH system requires signal synchronization to synchronize incoming ethernet signal with GFP frame. The foreign nation research completes a chipset development until now and it secures a relation technique, but it does not secure a relation technique from domestic. Therefore, in this paper, we presented with signal synchronization method of Ethernet signal through GFP frame. We knew that the synchronized method of Ethernet signal through GFP-F must apply ingress & egress buffer and GFP Idle. We understood that the synchronized method of Ethernet signal through GFP-T must apply GFP Idle and $65B{\_}PAD$, and require maximum 3-bit addition & deletion of idle. Also we showed signal synchronization realization through simulation and obtained MTIE/TDEV characteristics and peak to peak jitter in egress output.

• Biomedical Science Letters
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• v.7 no.4
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• pp.205-210
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• 2001

Whereas apoptosis is a critical mode of cell deletion in normal organism development, apoptotic cells are also observed in tumor therapy. We therefore investigated the expression of apoptotic cells induced as a function of time and dose in murine A-20 lymphoma treated with cyclophosphamide in vivo, by H&E and TUNEL method. The percent of apoptotic cells were scored from tumor section using TUNEL method. The expression of apoptotic positive cell was determined over a 10-day period following treatment of the mice with 200 mg/kg. Apoptosis increased further with time, reaching a peak value between 12~24 hr (scored 6.7$\pm$1.0%~6.1$\pm$0.7%), and then slowly declined to background levels by 10 days after treatment. The dependence of induction of apoptosis on the dose of cyctophosphamide was determined by treatment with 50, 100, or 200 mg/kg at 12 hr after treatment. Apoptosis was dose dependent in that as the dose was increased the percentage of apoptosis increased. However, the increase in apoptosis at the lower dose used (50 mg/kg) was higher on a per unit dose basis than that at the higher dose used (200 mg/kg). This result show that the alkylating agent cyclophosphamide strongly induces apoptosis in murine lymphoma.

• Journal of Information Technology Services
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• v.3 no.1
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• pp.21-41
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• 2004

The technical proposal suggested and published by MIC(Ministry of Information and Communication, henceforth MIC) contains too general assessment elements, which causes qualitative and subjective assessment of technical proposals. Thus, in terms of the technical proposal assessment. It lacks In fairness, validity and accuracy. Furthermore, it has a great deal of difficulty in assessment caused by the inconsistency between proposal planning and assessment methods. Also, each company has different writing format, so it is impossible to make use of its maneuvering data for the assessment. To overcome these weaknesses, our research focused on a quantitative evaluation on development organization, which is a part of organizational and administrative part of the technical proposal suggested and published by MIC. In this research, we divided development organization for the technical proposal into organization, teams, and team members, and then studied addition, deletion and merging for the assessment criteria. For the related study, we chose especially CMM(Capability Maturity Model) from a lot of international and national references, which is a model measuring the maturity of organization, and then we focused on Small-CMM which is available in the small-sized organization. We also suggested input form, description method, assessment elements for the quantitative assessment in the chosen developmental organization, and finally we proposed standard referencing criteria for the assessment. Our study concludes that our assessment method are valid and available in comparison with the previous Delphi method through a validity evaluation test.

• Journal of Advanced Marine Engineering and Technology
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• v.37 no.2
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• pp.227-235
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• 2013

The quality of the part-of-speech (POS) annotation in a corpus plays an important role in developing POS taggers. There, however, are several kinds of errors in Korean POS-tagged corpora like Sejong Corpus. Such errors are likely to be various like annotation errors, spelling errors, insertion and/or deletion of unexpected characters. In this paper, we propose a method for detecting annotation errors using error patterns, and also develop a tool for effectively correcting them. Overall, based on the proposed method, we have hand-corrected annotation errors in Sejong POS Tagged Corpus using the developed tool. As the result, it is faster at least 9 times when compared without using any tools. Therefore we have observed that the proposed method is effective for correcting annotation errors in POS-tagged corpus.

• Nuclear Engineering and Technology
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• v.35 no.2
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• pp.165-170
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• 2003

We developed an improved 3-PM liquid scintillation counting (3-PM LSC) method in which three detectors can be displaced to back and forth directions, and a data acquisition system being able to provide the values for all parameters required for the method. The detectors are entirely located in a 20-mm lead chamber of an inner diameter of 500 mm. A saw-toothed gear ties up all detectors so as to move them uniformly, up to 50 mm with unit of 1 mm. The data acquisition system was designed in an integrated circuit to perform the necessary works such as fast amplification, discrimination, coincidence and logic analysis. It generates values of nine parameters among twelve's generated in the 3-PH LSC method. The dead time of each counting channel is of extending type, valving from 10 to 100 $mutextrm{s}$. We measured the TDCR values with an unquenched liquid scintillation source 1"C by displacing the detectors with a step of 2.5 mm away from counting vial. Their values were derived on the range from 0.9 to 0.6. The extent is three times wider than those regions observed by applying the defocalization technique.ique.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.3D
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• pp.317-323
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• 2008

Mass transit information can contribute many benefits to users. Especially, transportation information technology is developing highly with information technology in Korea recently. Hereafter, it is expected to give customized transportation information to users individually with the advent of ubiquitous age in earnest. This public transportation information service can be realized by path finding algorithm in public transportation networks including travel and transfer attributes. In this research, constraints are constructed with the primary facts influencing users. Moreover, the method reducing user's path finding condition arbitrarily is proposed by making the maximum value as variables. In this study, transfer frequency, total travel time, seat confirmation, transfer time and travel time become constraint condition based on k path finding algorithm considering service time constraint condition. Moreover, case study about user customized transfer information is performed in Seoul and metropolitan subway networks.