• 제목/요약/키워드: Degree of Generation

검색결과 650건 처리시간 0.035초

DNA 표지에 의한 채종원내 소나무 교배양식 구명 (Mating System in Seed Orchard of Japanese Red Pines Revealed by DNA Markers)

  • 홍용표;김영미;안지영;박재인
    • 한국산림과학회지
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    • 제99권3호
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    • pp.344-352
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    • 2010
  • 소나무 채종원내 클론 간 교배양식을 구명하기 위해서 4개의 nSSR 표지와 6개의 cpSSR 표지를 이용하여 소나무 채종원 내에서의 타가교배율, 기여 화분친 수, 화분오염율을 산출하였다. cpSSR haplotype에 근거한 타가교배율은 94~100%로, 평균 98.9%의 타가교배율이 산출되었다. nSSR genotype에 근거한 타가교배율은 90.3~100%로, 평균 95.9%의 타가교배율이 산출되었다. 별개의 DNA 표지분석에서 자가교배로 확인 되었던 종자들을 두 표지를 동시에 비교하여 확인한 결과 타가교배 종자로 최종 확인되었다(누적 타가교배율 100%). 화분오염율은 최소 43.6%(강원10)에서 최대 56.4%(강원12)로 평균 48.9%로 계산되었다. 종자의 cpSSR haplotype을 근거로 확인한 기여 화분친은 경북38에서 21개로 최대치가 확인되었으며, 강원10에서 13개로 최소치가 확인되어 평균 16.2개의 기여 화분친이 확인되었다. 결론적으로, 안면도 소나무 채종원 ’77단지내 클론간 높은 타가교배율이 확인됨으로써 채종원산 종자의 유전적 안정성을 기대할 수 있으며, 안면도 소나무 채종원 '77단지 내 교배양식 분석을 통해서 확인된 결과가 향후 전진세대 채종원 조성 및 관리에 유용한 정보를 제공할 것으로 기대된다.

Enhanced photocatalytic oxidation of humic acids using Fe3+-Zn2+ co-doped TiO2: The effects of ions in aqueous solutions

  • Yuan, Rongfang;Liu, Dan;Wang, Shaona;Zhou, Beihai;Ma, Fangshu
    • Environmental Engineering Research
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    • 제23권2호
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    • pp.181-188
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    • 2018
  • Photocatalytic oxidation in the presence of Fe-doped, Zn-doped or Fe-Zn co-doped $TiO_2$ was used to effectively decompose humic acids (HAs) in water. The highest HAs removal efficiency (65.7%) was achieved in the presence of $500^{\circ}C$ calcined 0.0010% Fe-Zn co-doped $TiO_2$ with the Fe:Zn ratio of 3:2. The initial solution pH value, inorganic cations and anions also affected the catalyst photocatalytic ability. The HAs removal for the initial pH of 2 was the highest, and for the pH of 6 was the lowest. The photocatalytic oxidation of HAs was enhanced with the increase of the $Ca^{2+}$ or $Mg^{2+}$ concentration, and reduced when concentrations of some anions increased. The inhibition order of the anions on $TiO_2$ photocatalytic activities was $CO{_3}^{2-}$ > $HCO_3{^-}$ > $Cl^-$, but a slightly promotion was achieved when $SO{_4}^{2-}$ was added. Total organic carbon (TOC) removal was used to evaluate the actual HAs mineralization degree caused by the $500^{\circ}C$ calcined 0.0010% Fe-Zn (3:2) co-doped $TiO_2$. For tap water added with HAs, the $UV_{254}$ and TOC removal rates were 57.2% and 49.9%, respectively. The $UV_{254}$ removal efficiency was higher than that of TOC because of the generation of intermediates that could significantly reduce the $UV_{254}$, but not the TOC.

Exploiting cDNA Microarray-Based Approach Combined with RT-PCR Analysis to Monitor the Radiation Effect: Antioxidant Gene Response of ex vivo Irradiated Human Peripheral Blood Lymphocyte

  • Sung, Myung-Hui;Jun, Hyun-Jung;Hwang, Seung-Yong;Hwang, Jae-Hoon;Park, Jong-Hoon;Han, Mi-Young;Lee, U-Youn;Park, Eun-Mi;Park, Young-Mee
    • 한국환경성돌연변이발암원학회지
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    • 제22권3호
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    • pp.142-148
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    • 2002
  • Although ionizing radiation (IR) has been used to treat the various human cancers, IR is cytotoxic not only to cancer cells but to the adjacent normal tissue. Since normal tissue complications are the limiting factor of cancer radiotherapy, one of the major concerns of IR therapy is to maximize the cancer cell killing and to minimize the toxic side effects on the adjacent normal tissue. As an attempt to develop a method to monitor the degree of radiation exposure to normal tissues during radiotherapy, we investigated the transcriptional responses of human peripheral blood lymphocytes (PBL) following IR using cDNA microarray chip containing 1,221 (1.2 K) known genes. Since conventional radiotherapy is delivered at about 24 h intervals at 180 to 300 cGy/day, we analyzed the transcriptional responses ex-vivo irradiated human PBL at 200 cGy for 24 h-period. We observed and report on 1) a group of genes transiently induced early after IR at 2 h, 2) of genes induced after IR at 6 h, 3) of genes induced after IR at 24 h and on 4) a group of genes whose expression patters were not changed after IR. Since Biological consequences of IR involve generation of various reactive oxygen species (ROS) and thus oxidative stress induced by the ROS is known to damage normal tissues during radiotherapy, we further tested the temporal expression profiles of genes involved in ROS modulation by RT-PCR. Specific changes of 6 antioxidant genes were identified in irradiated PBL among 9 genes tested. Our results suggest the potential of monitoring post-radiotherapy changes in temporal expression profiles of a specific set of genes as a measure of radiation effects on normal tissues. This type of approach should yield more useful information when validated in in vivo irradiated PBL from the cancer patients.

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BES 기법을 이용한 육계사 내부 고온 스트레스 평가를 위한 THI 지수 모의 (Estimation of THI Index to Evaluate Thermal Stress of Animal-occupied Zone in a Broiler House Using BES Method)

  • 하태환;권경석;홍세운;최희철;이준엽;이동현;우샘이;양가영;김락우;여욱현;이상연;이인복
    • 한국농공학회논문집
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    • 제60권2호
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    • pp.75-84
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    • 2018
  • Thermal stress of livestock has been issued due to recent climate change trends and this causes reproductive disorders, decreased feed consumption, immunosuppression, and increased mortality of animals. Concept of THI has been widely used to quantitatively evaluate the degree of thermal stress for animals, however use of this concept is restricted for animals living in the enclosed facilities such as mechanically ventilated broiler houses. In this study, time-based internal energy flow and variation trends of temperature and humidity were analyzed based on BES technique. Local weather data, insulation characteristics of building materials, heat and moisture generation rate from broilers according to age, algorithm of ventilation operation were adopted for boundary condition of the model to accurately compute THI values inside the mechanically ventilated broiler house. From the BES computation, excess frequency of THI threshold in Jeju city was highest on the assumption that air conditioning equipments were not installed. When general raising density ($39kg\;m^{-2}$) was adopted, total 2,191 hours were exceeded. Excess hours of THI threshold were strongly related to the cumulative air temperature ($R^2=0.87$).

기후변화에 따른 농업용수공급패턴의 변화로 인한 이수안전도변화분석 (Water Supply Reliability Revaluation For Agricultural Water Supply Pattern Changes Considering Climate Changes)

  • 최영돈;안종서;신현석;차형선
    • 한국수자원학회:학술대회논문집
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    • 한국수자원학회 2010년도 학술발표회
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    • pp.273-277
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    • 2010
  • This research was performed to examine changes in the timing of the growth of crops along with changes in temperatures due tochanges and to analyze the change of water-supply-reliability by adding an analysis of the change of agricultural water supply patterns in the basin area of Miryang dam in Korea. Had-CM3 model from U.K. was the tool adopted for the GCM model, a stochastic, daily-meteorology-generation-model called LARS-WG was alsoused for downscaling and for the climate change scenario (A1B) which represents Korea's circumstances best. First of all, to calculate changes in the timing of the growth of crops during this period, the theory of GDD was applied. Except for the period of transplanting and irrigation, there was no choice but to find the proper accumulated temperature by comparing actual temperature data and the supply pattern of agricultural use due to limited temperature data. As a result, proper temperatures were found for each period. $400^{\circ}C$ for the preparation period of a nursery bed, $704^{\circ}C$ for a nursery bed's period, $1,295^{\circ}C$ for the rice-transplanting period, $1,744^{\circ}C$ for starting irrigation, and $3,972^{\circ}C$ for finishing irrigation. To analyze future agricultural supply patter changes, the A1B scenario of Had-CM3 model was adopted, and then Downscaling was conducted adopting LARS-WG. To conduct a stochastical analysis of LARS-WG, climate scenarios were generated for the periods 2011~2030, 2046~2065, 2080~2099 using the data of precipitation andMax/Min temperatures collected from the Miryang gauging station. Upon reviewing the result of the analysis of accumulated temperatures from 2011~2030, the supply of agricultural water was 10 days earlier, and in the next periods-2046~2065, 2080~2099 it also was 10 days earlier. With these results, it is assumed that the supply of agricultural water should be about 1 month ahead of the existing schedule to meet the proper growth conditions of crops. From the results of the agricultural water supply patterns should be altered, but the reliability of water supply becomes more favorable, which is caused from the high precipitation change. Furthermore, since the unique characteristics of precipitation in Korea, which has high precipitation in the summer, water-supply-reliability has a pattern that the precipitation in September could significantly affect the chances of drought the following winter and spring. It could be more risky to make changes to the constant supply pattern under these conditions due to the high uncertainty of future precipitation. Although, several researches have been conducted concerning climate changes, in the field of water-industry, those researches have been solely dependent on precipitation. Even so, with the high uncertainty of precipitation, it is difficult for it to be reflected in government policy. Therefore, research in the field of water-supply-patterns or evapotranspiration according to the temperature or other diverse effects, which has higher reliability on anticipation, could obtain more reliable results in the future and that could result in water-resource maintenance to be safer and a more advantageous environment.

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농약의 사용과정과 중독에 관한 사회의학적 고찰 (A Socio-medical Study on the Usage and Poisoning of Pesticides)

  • 차민영;차형훈;염용태
    • 농촌의학ㆍ지역보건
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    • 제9권1호
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    • pp.18-26
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    • 1984
  • With the increase of using pesticides, pesticide poisonings become more frequent. In the previous epidemiological study on pesticide poisoning and the reports of the authorities concerned, it is carefully affirmed that pesticide poisonings are caused by farmers' ignorance and negligence in using the pesticides. But the pesticide poisoning should be taken into account in terms of the person who sprays the pesticides, its spraying process, farming style and farmers' socio-economic conditions. And based on the understanding mentioned above, preventive measures for pesticide poisoning should be established. In this socio-medical point of view, this study examined the public health problems caused by using pesticides and the treatment of pesticide poisoning, based on the interviews with farmers in a area of Kyunggi-Do about the spraying process of pesticides, poisoning experiences and its treatments. The results are as follows ; 1) The style of spraying pesticides in the target area is mostly an individual one, not cooperative one. And the subjects of spraying are householders, whose educational backgrounds are beneath notice. More than 2~5 of the subjects are women or old men of 60 years old or so. These are seen to derive from the socio-economic status of Korean agricultural families; their younger generation's moving to greater cities and their petty farming styles. 2) As for the safety measures for the spraying of pesticides, those which have nothing to do with the economic, problems such as efficient spraying and productivity, are well obeyed. On the other hand, some safety measures as the use of safety devices, and spraying time, the degree of dilution of pesticides and the spraying method In windy days, are not obeyed very well, for to keep those makes spraying difficult to do and productivity worse. These facts indicate that the safety measures for using pesticides must be directly concerned with the spraying process, farming style and farmers' socio economic status, rather than the subject's knowledge or carefulness. 3) Even In cases of poisoning they do rarely consult the doctors. This is caused by the problem of their locality and transportation since one should pass away a day even in the busiest season to consult doctors, and by their low socio-economic status. 4) The epidemiological studies on pesticide poisonings should be necessarily led to a socio-medical one which are concerned together with the spraying process of pesticides, farming style, farmer's socio-economic status and discrepancies in public health policies between agricultural communities and urban ones.

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지질산화생성물의 DNA손상작용 및 그 억제기구 (DNA Damage of Lipid Oxidation Products and Its Inhibition Mechanism)

  • 김선봉;강진훈;박영호
    • 한국수산과학회지
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    • 제20권5호
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    • pp.419-430
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    • 1987
  • 지질산화생성물에 의한 DNA손상작용 및 그 억제 기구를 밟히기 위하여 linoleic acid와 plasmid DNA와의 모델계를 통하여 검토하였는데, 그 결과를 요약하면 다음과 같다. 1. Linoleic acid의 산화에 의하여 DNA가 손상되었으며, 그 정도는 linoleic acid의 양이 많을 수록 크게 나타났다. 2. Linoleic acid의 산화에 의한 DNA손상작용은 POV 100 mea/kg이하인 산화초기에서도 빠르게 진행되었다. 산화초기의 DNA손상작용에는 활성산소 종의 관여가 크게 나타났는데, 그 중에서도 일중항 산소와 superoxide anion의 영향이 큰 것으로 나타났다. 3. 지질 2차반응생성물인 malonaldehyde와 hexanal의 DNA손상작용은 linoleic acid경우와는 달리 활성산소종과는 무관하였으며 DNA와 와의 복합체형성에 의하였다. 4. Linoleic acid hydroperoxide의 DNA 손상작용은 linoleic acid의 초기신화에 의한 DNA손상작용 보다 크게 나타났고, 활성산소종의 영향은 없었다. 5. 지진산화생성물에 의한 DNA손상 작용은 천연 항산화성분(마늘 및 생강추출물) 및 활성산소소거제($\alpha-tocopherol 및 superoxide dismutase$의 첨가에 의하여 크게 억제되었다. 특히 . 마늘 및 생강추출물은 활성산소종의 생성을 비롯하여 공액 diene 및 POV의 증가 또한 크게 억제하였다.

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쓰로틀밸브 급개방시 기류소음의 4극음원에 대한 정량적 해석 (Quantitative Analysis of Quadrupole Noise Sources upon Quick Opening The Throttle)

  • 김재헌;정철웅;김성태;이수갑
    • 한국음향학회:학술대회논문집
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    • 한국음향학회 2002년도 하계학술발표대회 논문집 제21권 1호
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    • pp.469-474
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    • 2002
  • In recent years, modularization of engine parts has increased the application of plastic products in air intake systems. Plastic intake manifolds provide many advantages including reduced weight, contracted cost, and lower intake air temperatures. These manifolds, however, have some weakness when compared with customary aluminium intake manifolds, in that they have low sound transmission loss because of their lower material density. This low transmission loss of plastic intake manifolds causes several problems related to flow noise, especially when the throttle is opened quickly. The physical processes, responsible for this flow noise, include turbulent fluid motion and relative motion of the throttle to the airflow. The former is generated by high-speed airflow in the splits between the throttle valve and the inner-surface of the throttle body and surge-tank, which can be categorized into the quadrupole source. The latter induces the unsteady force on the flow, which can be classified into the dipole source. In this paper, the mechanism of noise generation from the turbulence is only investigated as a preliminary study. Stochastic noise source synthesis method is adopted for the analysis of turbulence-induced, i.e. quadrupole noise by throttle at quick opening state. The method consists of three procedures. The first step corresponds to the preliminary time-averaged Navier-Stokes computation with a $k-\varepsilon$ turbulence model providing mean flow field characteristics. The second step is the synthesis of time-dependent turbulent velocity field associated with quadrupole noise sources. The final step is devoted to the determination of acoustic source terms associated with turbulent velocity. For the first step, we used market available analysis tools such as STAR-CD, the trade names of fluid analysis tools available on the market. The steady state flows at three open angle of throttle valve, i.e. 20, 35 and 60 degree, are numerically analyzed. Then, time-dependent turbulent velocity fields are produced by using the stochastic model and the flow analysis results. Using this turbulent velocity field, the turbulence-originated noise sources, i.e. the self-noise and shear-noise sources are synthesized. Based on these numerical results, it is found that the origin of the turbulent flow and noise might be attributed to the process of formulation and the interaction of two vortex lines formed in the downstream of the throttle valve. These vortex lines are produced by the non-uniform splits between the throttle valve and inner cylinder surface. Based on the analysis, we present the low-noise design of the inner geometry of throttle body.

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전자상거래 평가모형에 관한 연구 : 인터넷 비즈니스모델을 중심으로 (Study on E-commerce Evaluation Model : Focused on "Internet Business Model")

  • 이영민
    • 유통과학연구
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    • 제14권1호
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    • pp.85-91
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    • 2016
  • Purpose - Recently, the importance of rapid change in business models is more and more increasing as the change of information technology environment. Therefore, a variety of business models have emerged. On the other hand, there is no company that can generate revenue. Many enterprises are still maintained while they are changing only their appearance of the business model. Business model is important in e-commerce. However, a lot of researches are targeted only in Web sites. Thus, e-commerce companies do not have the infrastructure for measuring and business models. The purpose of paper is to evaluate factors which are related with the structuring of the e-commerce success. And it proposed a financial items and non-financial items. From the perspectives of administrators and managers, the paper researches the possibility for E-Commerce Evaluation Model as a valuable criteria in measuring business model. Research design, data and methodology - The methods are taken by the classification for the type of business-to-business transactions, transactions subject, and the degree of integration and innovation capabilities. Financial and Non-financial value is used to build E-Commerce Evaluation Model. Evaluation items in Administration's perspective are composed with enhance the effectiveness of the mission, improving efficiency of the administration, and control of costs. Evaluation items in the customer's perspective were measured by customer participation and cooperation with customer Satisfaction. In the case of researching the information system's perspective, three criteria are used such as adequacy of the development process, improvement of the quality of service, and maintenance of standardized information technology. In researching for the ICT competence's perspective, evaluation items were composed of enhanced user capabilities, utilizing new technologies, and empowerment of information workers. Results - In this paper, E-Commerce Evaluation Model with financial and non-financial perspectives shows the possibility to be criteria in the case of measuring business model. Moreover, it gives the positive expectation to be successful criteria. But the research may have ambiguity in its essential concept because it cannot avoid the limitation in selecting evaluation tools from merely the model. It is impossible to exclude the possibility in omitting specific properties which may take place in actual case study. Therefore, In hereafter research, it is necessary to include actual case study research in selecting evaluation tools in order to improve the limit point. Actual measurement items which are derived from actual case study should be subdivided, and it would be more effective to complete the research. Conclusions - In rapid change in business models, there are various kinds of business models. But it is general situation that companies which adopted business models have not brought in revenue. For this reason, E-Commerce Evaluation Model is needed as an important factor for the structuring of the e-commerce success. Although it has the limitation in selecting evaluation tools from model, E-Commerce Evaluation Model proposes the implication for measuring business models as a valuable criteria.

하중 분산형 앵커 내하체에 대한 모노타입 하중계의 현장 적용성 평가 (Field Applicability Evaluation of Monotype Load Cell for Load-Distributing Anchor)

  • 송용재;이강일;장용채;김상용
    • 한국지반신소재학회논문집
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    • 제23권3호
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    • pp.23-30
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    • 2024
  • 건설 구조물을 지반에 정착시켜 구조물을 안전하게 유지하기 위한 그라운드 앵커공법은 정착 방식에 따라 하중분산형 앵커와 일반형 앵커로 구분된다. 최근 현장에서는 앵커의 인장력이 크게 나타나는 하중분산형 앵커를 적용하는 사례가 증가하는 추세이지만 그에 따른 현장 적용성에 대한 문제점도 있다. 하중분산형 앵커는 강연선에 인장력을 각각 다른 구간에 정착시켜 가하는 방식으로 앵커체의 길이 차이로 인해 정착장 별 최대 인장력 크기가 다르게 나타나는 편하중의 문제점이 발생하고 있다. 이에 본 연구에서는 앵커의 편하중에 따른 품질관리 방안으로 하중분산형 앵커의 각 강연선별로 하중 측정이 가능한 모노타입 하중계를 개발하였으며, 기존의 멀티타입 하중계와 모노타입 하중계의 측정 결과를 비교 분석하여 현장 적용성을 분석하였다. 그 연구 결과, 멀티타입 하중계는 내하체별 작용하중을 추정할 수 밖에 없어 하중분산형 앵커의 강연선별 편하중 발생에 따른 관리는 불가능하였다. 그러나 모노타입 하중계의 경우에는 각 내하체별, 강연선별로 하중이 측정되어 지반조건, 시공조건 등에 상관없이 각 강연선별로 하중값이 측정되어 편하중 발생에 따른 안전관리와 시공관리가 가능할 것으로 판단된다.