• Asian-Australasian Journal of Animal Sciences
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• v.2 no.4
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• pp.583-590
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• 1989

Increasing levels of ammonia-N in the rumen fluid used for in vitro incubation were achieved by supplementation of the ration of the donor cows with urea and by addition of urea either with or without glucose to the rumen fluid after collection. The ration of the donor animals consisted of wheat straw (80%) and maize silage (20%). During the second half of the experiment the basal ration was supplemented with a mineral mixture. Wheat straw, Guinea grass and two rice straw varieties were incubated with the various kinds of rumen fluid. Parameters studied were: solubility, apparent organic matter disappearance after 48 hours of incubation ($OMD_{48}$), rate of organic matter degradation from 0 to 24 hours of incubation ($k_1$) and from 24 to 95 hours ($k_2$). The concentration of ammonia-N in the rumen fluid at which 95% of the maximal $OMD_{48}$ and k1 were reached (88.2 and 100.0 mg/l) were independent of the feed. With regard to the $k_2$ the required ammonia-N concentration to reach 95% of the maximal $k_2$ differed per feed. Mineral supplementation increased the OMD48 and $k_1$, but not the solubility and $k_2$. Glucose addition in combination with urea had no beneficial effect compared to urea supplementation alone.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.6
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• pp.593-598
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• 1997

The changes of tissue structure in timothy and alfalfa during ensiling process with silage additives; lactic acid bacteria, cellulase and formic acid, were observed with a video microscope. Stem samples were obtained from the second internode, and cut to divide into 2 pieces. One piece was for observation of ensiled material and the other was for silage. The latter piece was put into a nylon cloth bag, and ensiled with grass for 50 days in a small experimental silo Lignification of the plant tissues was checked by acid phloroglucinol. Natural silage fermentation resulted in some degradation of less lignified parenchyma in both plant species. However, lignified sclerenchyma and vascular bundles remained intact. The cellulase enhanced the degradation of parenchyma tissue, while the formic acid suppressed the degradation. The effect of lactobacillus was small. The percentage of remained cross sectional area of stem and the loss of NDF and ADF by silage fermentation confirmed the observation. High negative correlations were obtained between the remained area and loss of fibrous components during silage fermentation in both plants, and between the loss of fibrous components and in vitro dry matter digestibility in timothy but not in alfalfa.

• Journal of Korean Society of Forest Science
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• v.98 no.5
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• pp.609-617
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• 2009

This study was conducted to investigate ecological indicators of forest degradation after forest fire and clear-cutting in the Siberian larch (Larix sibirica Ledeb.) stand of Mongolia. The species abundance and biodiversity indices were higher in burned and clear-cut stands than those of reference stand, but boreal understory species, such as Vaccinium vitis-idaea, Pyrola incarnata, Linnea borealis and Maianthemum bifolium, completely disappeared and was replaced by sedge species, such as Carex duriuscula, C. lanceolata, C. pediformis, Poa attenuata and P. pratensis. During the research period, temperature increased by an average of $1.6^{\circ}C$ in burned stand and $1.7^{\circ}C$ in clear-cut stand compared to reference stand, but RH sharply decreased up to 15.7% in clear-cut stand. This result indicates that Larix sibirica stand became warmer and drier after forest fire and clear-cutting, and contributed to the abundance of sedge and grass species in the understory. Moreover, intense occupation of tall sedge grass after forest fire and clear-cutting had a vital role as obstacle on natural regeneration of Larix sibirica. The similarity of species composition between reference and burned stands was higher (73.6%) than between reference and clear-cut stands (63.8%). Soil moisture significantly decreased after forest fire and clear-cutting, and the extent of decrease was more severe in the clear-cut stand. The chemical properties at soil organic layer were significantly affected by forest fire and clear-cutting but not the mineral horizons. Inorganic nitrogen of the forest floor significantly decreased in the clear-cut stand ($1.1{\pm}0.4mg{\cdot}kg^{-1}$) than that of the burned ($4.5{\pm}2.3mg{\cdot}kg^{-1}$) and reference stands ($5.0{\pm}2.3mg{\cdot}kg^{-1}$). Available P of the forest floor significantly increased after fire, whereas it decreased after clear-cutting. These results indicate that existence of boreal understory vegetation, and changes in soil moisture and available P are distinct attributes applicable as ecological indicators for identifying forest degradation in Mongolia.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.28 no.7
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• pp.456-461
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• 2015

In this study, the frequency properties of electrostatic capacity and dielectric loss for the samples with different types of filler has been measured in through the applied frequency range of 7 kHz ~3,000 kHz at temperature of $80^{\circ}C$, $110^{\circ}C$, $140^{\circ}C$, $170^{\circ}C$. The results of this study are as follows. When the sample is degradated at the temperature of $80^{\circ}C$, $110^{\circ}C$, $140^{\circ}C$, $170^{\circ}C$ and the frequency range of 7 kHz ~3,000 kHz is applied, It found that the electrostatic capacity of the sample with Polyimide film is larger than the sample with Grass fiber. It found that the dielectric loss for the sample with Polyimide film is larger than the sample with Grass fiber with increasing frequency and temperature in the $80^{\circ}C$, $110^{\circ}C$, $140^{\circ}C$, $170^{\circ}C$ range. Also, the dielectric loss decreased with increasing frequency. In case of the sample with Polyimide film, It found that the electrostatic capacity decreased with increasing temperature, and the dielectric loss gradually decreased with increasing frequency.

• Asian Journal of Turfgrass Science
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• v.7 no.1
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• pp.31-34
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• 1993

There is a growing concern in the United Stares over the environmental and human health implications associated with heavy use of water, pesticides, and inorganic ferilizers in maintaining picture perfect golf courses. There is also a growing awareness that a beautiful course is not necessarily a healthy course. The following discussion reviews the interrelationship of turfgrass and the soil that supports it and provides basic information on currently available alternatives to turf management practices that feature intensive application of inorganic fertilizers. water and pesticides. Soil is a dynamic natural environment in which microorganisms play an important role. Soil contains a large mass of microorganisms which produce thousands of enzymes that can catalyze the transformation and degradation of many organic molecules. (In top soil under optimum conditions may contain 10 billion cells per gram of soil.). Turfgrass and the soil which supports it are interdependent. The natural organic cycle as applied to turf and soil begins with healthy vigorous grass plants storing up the sun's energy in green plant tissues as chemical energy. Animals obtain energy by eating plants and when plants and animals die, their wastes are returned to the soil and provide "food" for soil microorganisms. In the next step of the organic cycle soil microorganisms break down complex plant tissues into more basic forms and make the nutrients available to grass roots. Finally, growing plants extract the available nutrients from the soil. By free operation of this organic cycle, natural grasslands have some of the most fertile soils on earths.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1460-1468
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• 2003

An experiment was conducted to quantify the flow of soluble non-ammonia nitrogen (SNAN) in the liquid phase of ruminal (RD) and omasal digesta (OD), and to investigate diurnal pattern in SNAN flow in OD. Five ruminally cannulated Finnish-Ayrshire dairy cows in a $5{\times}5$ Latin square design consumed a basal diet of grass silage and barley grain, and that supplemented with four protein feeds (kg/d DM basis) as follows: skimmed milk powder (2.1), wet distiller' solubles (3.0), untreated rapeseed meal (2.1) and treated rapeseed meal (2.1). Ruminal digesta was sampled using a vacuum pump, whereas OD was collected using an omasal sampling system at 1.0 h interval during a 12 h feeding cycle. Both RD and OD were acidified, centrifuged to remove microbes and precipitated with trichloroacetic acid followed by centrifugation. The SNAN fractions (free amino acid (AA), peptide and soluble protein) in RD and OD were assessed using ninhydrin assay. Free AA, peptide and soluble protein averaged 60.0, 89.4 and 2.1 g/d, respectively, for RD, and 81.8, 121.5 and 2.5 g/d, respectively, for OD. Although free AA flow was relatively high, mean peptide flow was quantitatively the most important fraction of SNAN, indicating that degradation of peptide to AA rather than hydrolysis of soluble protein to peptide or deamination may be the most limiting step in rumen proteolysis. Diurnal pattern in flow of peptide including free AA in OD during a 12 h feeding cycle peaked 1 h post-feeding, decreased by 3 h post-feeding and was relatively constant thereafter. Protein supplementation showed higher flow of peptide including free AA immediately after feeding compared with no supplemented diet. There were no differences among protein supplements in diurnal pattern in flow of peptide including free AA in OD.

• Applied Biological Chemistry
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• v.31 no.2
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• pp.200-204
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• 1988

This study was conducted to evaluate effect of soil texture, organic matter, temperature and water regime on degradation of pretilachlor (2-chloro-2',6'-diethyl-N-(n-propoxyethyl) acetanilide) in the soils. The period of residual activity in soil treated with pretilachlor at rate of 60g a.i./10a was about 50 days. Also, period of reaching GR 50 value on the barnyard grass was about $25{\sim}27$ days. Degradation rate of pretilachlor in the sandy clay loam soil which has higher contents of organic matter and clay was faster than in the sandy loam soil. When organic matter was added to the soil, the rate of decomposition was accelerated. The faster degradation occurred under the $30^{\circ}C$ soil temperature by comparison under the $20^{\circ}C$. The rate of degradation in the soil was faster under the flooded conditions than under the wetted conditions.

• The Korean Journal of Pesticide Science
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• v.4 no.2
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• pp.21-28
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• 2000

A new fourty six 2-(4-(6-chloro-2-benzoxazolyloxy)phenoxy)-N-phenylpro- pionamide derivatives were synthesized and the herbicidal activities against rice plant and barnyard grass with pre-emergence in down land were measured. The structure activity relationships (SAR) between the activities and physicochemical parameters of the substituted(X) N-phenyl group in substrates were analyzed and discussed by Free- Wilson and Hansch method from the basis on the former study (Sung. et. al., 1999). The conditions of selective herbicide activity both the barnyard grass and rice plant are shown that the optimal hydrophobicity, $({\pi})_{opt.}=1.34$ and electron donating with field effect (F<0) of meta and ortho, para-substituted mono or disubstituent on the N-phenyl ring were found to contribute significantly. The herbicidal activities against barnyard grass are roughly the same as the results in up land whereas damage to rice plant in down land more increase than that of up land. Degradation products in water are 2-(4-(6-chloro-2-benzoxazolyloxy)phenoxy)propionic acid ((A)) (obs. pKa=4.35 & obs. logP=4.77) and 6-chloro-2-benzoxazolone (B) (obs. pKa=8.40 & obs. logP=2.90). These results were supposing that the hydrolysis product of substrates, (A) is comparatively absorbed in rice plant but not in barnyard grass. And it is assumed from the SAR equations that the 2,6-dimethyl-4-methoxymethyl group substituent ($pI_{50}=5.41$, 3g/ha) is selected as the most highest herbicidal activity against barnyard grass in green house.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.2
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• pp.210-214
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• 1997

Ruminal degradation characteristics and intestinal availability of crude protein (CP) in four animal-origin feeds (fish meal, meat meal, viscera meal, feather meal) were estimated by mobile nylon bag technique. Three ruminally and duodenally cannulated Holstein dairy cows (average body wt. 550kg) fed a diet containing 40% concentrate and 60% orchard grass hay on a dry matter (DM) basis. Assuming that the outflow rate of diet in rumen is 5% per hour (k =0.05), contents of quickly degradable CP (QDP), slowly degradable CP (SDP), and undegradable CP (UDP) in the rumen were 27.6%, 9.4%, 63.0% for fish meal, 34.3% 28.1%, 37,6% for meat meal, 43.9%, 12.5%, 43.6% for viscera meal, and 14.4%, 15.8%, 69.8% for feather meal, respectively. Intestinal CP degradability was 51.0% for fish meal, 27.2% for meat meal, 37.9% for viscera meal and 56.2% for feather meal. Available UDP in the intestinal tract was contained 288 g, 217 g, 246 g and 423 g per kilogram DM of diet in fish meal, meat meal, viscera meal and feather meal, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.11
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• pp.1686-1694
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• 2019

Objective: Lignin plays a relevant role in the inhibition of cell wall (CW) structural carbohydrate degradation. Thus, obtaining accurate estimates of the lignin content in tropical plants is important in order to properly characterize the mechanism of lignin action on CW degradation. Comparing conflicting results between the different methods available for commercial use will bring insight on the subject. This way, providing data to better understand the relationship between lignin concentration and implications with tropical forage degradation. Methods: Five grass species, Brachiaria brizantha cv $Marand{\acute{u}}$, Brachiaria brizantha cv $Xara{\acute{e}}s$(MG-5), Panicum maximum cv Mombaça, Pennisetum purpureum cv Cameroon, and Pennisetum purpureum cv Napier, were harvested at five maturity stages. Acid detergent lignin (ADL), Klason lignin (KL), acetyl bromide lignin (ABL), and permanganate lignin (PerL) were measured on all species. Lignin concentration was correlated with in vitro degradability. Results: Highly significant effects for maturity, lignin method and their interaction on lignin content were observed. The ADL, KL and ABL methods had similar negative correlations with degradability. The PerL method failed to reliably estimate the degradability of tropical grasses, possibly due to interference of other substances potentially soluble in the $KMnO_4$ solution. Conclusion: ADL and KL methods use strong acid ($H_2SO_4$) and require determination of ash and N content in the lignin residues, therefore, increasing time and cost of analysis. The ABL method has no need for such corrections and is a fast and a convenient method for determination of total lignin content in plants, thus, it may be a good option for routine laboratory analysis.