• Applied Biological Chemistry
• /
• 제47권4호
• /
• pp.430-433
• /
• 2004

Lilium cv. Casablanca pollen grains stored at $-70^{\circ}C$ were grown in pollen germination medium with Agrobacterium tumefaciens LBA4404 cells harboring pBI121 for 18 hr at $27^{\circ}C$. Following this, cefotaxime (250 mg/L) was treated for 6 hr to eradicate the bacterial cells. Histochemical GUS analysis revealed that the transgenic pollen displayed deep blue color mostly from 12 hr after the co-cultivation. Presence of $200\;{\mu}M$ acetosyringone was determined not to be more effective for GUS transformation than its absence. GUS DNA integration in the transgenic pollen genomic DNA was clearly demonstrated by Southern blot analysis.

• 천문학회보
• /
• 제35권1호
• /
• pp.55.2-55.2
• /
• 2010

We present a photometric and spectroscopic study of the globular clusters (GCs) in the giant elliptical galaxy NGC 4636 in the Virgo cluster. The photometry of the GCs is based on HST/WFPC2 images for the inner region and deep, wide field Washington $CT_1$ CCD images for the outer region. We investigated the color distribution, the radial number density profile, the spatial distribution, and the mean color of the bright blue GCs about the GCs in NGC 4636. We obtained spectra of the GC candidates in NGC 4636 using the Multi-Object Spectroscopy (MOS) mode of Faint Object Camera and Spectrograph (FOCAS) on the SUBARU 8.2m Telescope. We measured the velocities for 105 GCs in NGC 4636. Using the 238 GCs obtained from combining our results with data in the literature, we investigated the kinematics of the GC system of NGC 4636: the rotation, the velocity dispersion, the radial variation, and the orbit. We also investigated the distribution and the radial variation of the metallicities, ages, and alpha-elements of the GCs in NGC 4636 derived using the absorption lines. We compared the GC kinematics of NGC 4636 with those of other six gEs, finding that the kinematic properties of the GCs are diverse among gEs. We found several correlations between the kinematics of the GCs and the global parameters of their host galaxies. Finally we discuss the implication of the results for the formation models of the GC system in gEs, and suggest a mixture scenario for the origin of the GCs in gEs.