• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.287-292
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• 2002

Lignin-peroxidase (LiP) has been considered as one of the most important industrial enzymes for biodegradation of various recalcitrant toxic compounds such as chlorinated aromatic hydrocarbons and azo-dyes. Recently, several soil actinomycetes have been reported to secrete a functionally-similar lignin-peroxidase called actinomycetes lig-nin-peroxidase (ALiP). In this manuscript, we isolated over 100 morphologically distinct actinomycetes from the contaminated soils around 10 different gas stations located nearby the Kyung-An river. Among these actinomycetes screened based on the congo-red dye-decolorization activities, one newly-isolated actinomycetes named SMA-2 showed the most significant dye-decoloring activity on the congo-red plate as well as a significant ALiP activity in a yeast-extract-malt-extract liquid media supplemented with starch. The optimum SMA-2 culture condition fur ALiP production was determined and the kinetic parameters fur the SMA-2 AkIP activity were characterized. The optimally-cultured SMA-2 also exhibited the oxidation activities toward various recalcitrant aromatic compounds including phenol, 2- chlorophenol, 4- chlorophenol, 2,4- dichlorophenol ,2,6- dichlorophenol, and 2,4, f-trichlorophe - not, suggesting a potential application of SMA-2 for contaminated soil bioremediation.

• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.1
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• pp.68-74
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• 2002

This study was performed to examine the availability of electrolysis for removal of remaining biologically refractoty humus and residual color of leachate which is biologically pretreated in domestic waste matter sanitary landfill by recycling to landfill. The obtained results were as follows; 1) The electrolysis of leachate through covered bed represented that the removal efficiency of CODcr and color range from 70~80%, in color removal the only electrolysis for a treatment of leachate meet the critia of effluent. 2) The highest removal efficiency was represented in pH 7~8. 3) At anode used Al, Fe, Stainless the removal efficiency of CODcr and color was high in order of Fe, Al, stainless, in considering the settled ability of reaction product in economic or after electro coagulation the removal efficiency was highest when anode was the Fe electrode. 4) In this study conditions for removed both CODcr and Color ar the same time represented thar anode was used Fe, electrode-distance was 2cm and reaction time was 40min in 8volts.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07b
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• pp.818-821
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• 2003

Ozonic use of sterilization and heat treatment of raw material to extend the conservation period of food recently is increased by hard vegetable or microorganism sterilization purpose of fruit. If ozone can create as is easy comparatively because do air or oxygen by raw material and schedule period passes, there is advantage that do not leave the second contaminant because being gotten restored by oxygen. Also, because the effect is big to decolorization beside sterilization effect about microorganism, deodorization, disjointing of venomousness hazardous substance, food save, indoor air purge, good hand processing, hydrospace agricultural chemicals processing etc. the use extent is wide. This research ran parallel a sterilization experiment of E.coli colitis germs by ozone that manufacture ozonizer that use doped diamond maund electrode (BDD) by boron and searched special quality electrochemistry enemy of the ozonizer and is created. E.coli. After cultivate E.coli colitis germs during 37C 1 day is LB ship, after do ozonation, was sterilized more than 90% by ozone as result that examine sterilizing power by substance microscope and could confirm excellency of diamond electrode.

• Journal of Environmental Science International
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• v.17 no.8
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• pp.899-905
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• 2008

Quality test for activated carbon(AC) filter used for drinking water purifier is now an obligatory test and the standard material for valid purifying amount in water purifier performance test has been changed from residual chlorine to chloroform according to the notice of Ministry of Environment in 2006. Therefore, this study aimed to compare the ingredients of AC filters by confirming chloroform removal rate of AC filter and conducting 4 adsorption tests (Iodine, methylene blue decolorization, phenol value, ADS value) for AC filters provided by manufacturers. With water pressure of $1kgf/cm^2$, 1,500 liters of prepared inflow went through to check chloroform removal rate. As a result, product with removal rate of below 60% from all products. On the other hand, 4 adsorption tests were conducted for filters in the market and filters from manufacturers. None of the products satisfied all 4 tests. In particular, they showed great shortage to the standard in phenol value and ADS value test. However, manufacturers' filter showed much better performance than filters in the market. Also, the result of valid purifying amount test for each of five products of appropriate product and inappropriate product based on filter quality test showed average 4,440 liters for appropriate product and average 2,620 liters for inappropriate product. According to the result, it is shown that the filter with good adsorption also had good chloroform removal efficiency and adsorption efficiency. Therefore, it is expected that customers can screen good quality product through obligatory conduct of filter quality test. However, it is considered that complementation in system is required for future inspection.

• KSBB Journal
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• v.15 no.2
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• pp.150-155
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• 2000

Antioxidative activity of c비ture of Streptomyces sp. BH-405 was investigated. After removal of pellets of Streptomyces sp. B BH-405, antioxidative substances were is미ated and suc$\infty$sively purified from culture of Streptomyces sp. BH-405 by by thin | layer chromatography $\pi$LC) or silica gel column chromatography. The fraction 3 obtained from ethylether fractionation of the C culture appeared highest level of anti oxidative activity as determined by thiocyanate method. Band 2 obtained by further P purification of this fraction showed higher anti oxidation level than that of same concentration of dl- $\alpha$ -tocopherol, butylated h hydroxy anisole (BHA). The band 2 showed higher rate of 1, 1.diphenyl 2-picrylhydrazyl (DPPH) decolorization than dl-$\alpha$-tocopherol. In the rat liver microsomes, band 2 rapidly inhibited lipid peroxidation which was initiated enzymatically by r reduced nicotinamide adenine dinucleotide phosphate (NADPH) or non-enzymatically by Fenton’s reagent. Band 2 inhibited on | lipid peroxidation of mitochondria or the linoleic acid hydro peroxide induced peroxidation system. It is concluded that band 2 obtained by fractionation of Streptomyces sp. BH-405 cultivation contained antioxidants with the capacity to inhibit oxidative m modification.

• Journal of the Korean GEO-environmental Society
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• v.11 no.8
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• pp.41-47
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• 2010

This study was conducted to assess the applicability of pilot scale system and to evaluate the treatment efficiency on operational parameters such as humic acid concentration, initial pH and air flow rate on the ozone/peroxyl radical reaction system. The decolorization of ozone/peroxyl radical system was higher than that of only process. Removal efficiency of ozone/peroxyl radical system was generally increased with the increase of intial concentration of humic acid but decreased over the range of 30mg/L. Treatment efficiency of HA at acid pH was smaller compared to that of neutral or basic pH and increased with increasing the air flow rate from 1L/min to 3L/min. In pilot-scale test, average removal of TOC and $COD_{Cr}$ was about 70% and 60%, respectively and ozone/peroxyl radical reaction system was indicated a potential in water treatment application.

• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.412-412
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• 2014

Zinc oxide (ZnO) is one of the most powerful materials for purifying organic pollutants using photocatalytic activity. In this study, we have introduced a novel method to design highly photoreactive flexible 3 dimensional (3D) ZnO nanocomposite [F-ZnO-m (m: reaction time, min)] by electrospinning and simple-step ZnO growth processing (one-step ZnO seed coating/growth processing). Significantly, the F-ZnO-m could be a new platform (or candidate) as a photocatalytic technology for both morphology control and large-area production. The highest photocatalytic degradation rate ([k]) was observed for F-ZnO-m at 2.552 h-1, which was 8.1 times higher than that of ZnO nanoparticles (NPs; [k] = 0.316 h-1). The enhanced photocatalytic activity of F-ZnO-m may be attributed to factors such as large surface area. The F-ZnO-m is highly recyclable and retained 98.6% of the initial decolorization rate after fifteen cycles. Interestingly, the F-ZnO-m samples show very strong antibacterial properties against both Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) after exposure to UV-light for 30 min. The antibacterial properties of F-ZnO-m samples are more effective than those of ZnO NPs. More than 96.6% of the E. coli is sterilized after ten cycles. These results indicate that F-ZnO-m samples might have utility in several promising applications such as highly efficient water/air treatment and inactivation of pathogenic microorganisms.

• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1757-1764
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• 2013

Trichoderma harzianum ZF-2 producing laccase was isolated from decaying samples from Shandong, China, and showed dye decolorization activities. The objective of this study was to optimize its culture conditions using a statistical analysis of its laccase production. The interactions between different fermentation parameters for laccase production were characterized using a Plackett-Burman design and the response surface methodology. The different media components were initially optimized using the conventional one-factor-at-a-time method and an orthogonal test design, and a Plackett-Burman experiment was then performed to evaluate the effects on laccase production. Wheat straw powder, soybean meal, and $CuSO_4$ were all found to have a significant influence on laccase production, and the optimal concentrations of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for laccase production were determined as follows: wheat straw powder 7.63 g/l, soybean meal 23.07 g/l, $(NH_4)_2SO_4$ 1 g/l, $CuSO_4$ 0.51 g/l, Tween-20 1 g/l, $MgSO_4$ 1 g/l, and $KH_2PO_4$ 0.6 g/l. Using this optimized fermentation method, the yield of laccase was increased 59.68 times to 67.258 U/ml compared with the laccase production with an unoptimized medium. This is the first report on the statistical optimization of laccase production by Trichoderma harzianum ZF-2.

• Korean Journal of Food Science and Technology
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• v.16 no.4
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• pp.437-442
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• 1984

The color changes and sorption characteristics of dried whole red pepper stored at various relative humidity and temperature were studied. Dried whole red pepper was browned at relative humidity above 67%, and was molded at relative humidity above 84%, and was decolorated at relative humidity below 32%. Therefore, about 50% RH condition was suitable for the preservation of dried whole red pepper and the safe moisture content levels for storge to prevent decolorization were ranging from 15.65% to 19.62% dry basis (DB) with varying temperatures. The moisture contents of monolayer value for the dried whole red pepper were ranging from 7.52% to 9.23% (DB) with varying temperatures. The third order regression equation for the equilibrium moisture content prediction with relative humidity was determined.

• Korean Journal of Food Science and Technology
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• v.21 no.3
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• pp.425-429
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• 1989

Functional relationships of carotenoid destruction and nonenzymatic browning during red pepper drying were established by the dynamic test using the moisture-temperature-quality history curve in actual drying experiments. The dependence of the rate constants on temperature and moisture content was established and analysed assuming that carotenoid destruction and nonenzymatic browning are the first order and the zero order reaction, respectively. Carotenoid destruction rate constant was high at high moisture and high temperature, and had a minimum value at some intermediate moisture content. As dependence of rate constant on temperature, activation energy of carotenoid decolorization ranged from 7.7 to 27.4 kcal/mol, showing higher value at higher moisture content. Nonenzymatic browning showed higher rate at higher temperature and higher moisture content. Activation energy of browning was in the range of 7.5-20.2 kcal/mol with higher value at higher moisture level.