• Applied Biological Chemistry
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• v.13 no.1
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• pp.29-34
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• 1970

In order to prepare digested Protein source for the Weanling Food from soybean, an attempt was made to decompose steamed soybean protein to amino acids and peptides by protease and cellulase produced from Aspergillus niger and Aspergillus sojae. In this paper, the optimum condition for digestion of soybean protein were studied and also investigated the effects of decolorization of it. As the results, followings were obtained; 1. As steaming conditions, a treatment under 15 lb of pressure and 10 minutes of heating shows most effective. 2. The optimum pH of Asp, sojae enzyme for the digestion of soybean protein is 6.0, while that of Asp. niger enzyme is 4.4. In successievly-decomposing with Asp. sojae and Asp. niger, it shows the most effective on ratio of water-soluble-nitrogen to total nitrogen and amino-nitrogen to total nitrogen than any other separate treatments. 3. The suitable amount of the enzyme solution to that of the soybean substrate paste, in volume, is 1 : 2. 4. Digestion ratio of soybean protein indicates the gradual and steady effects of increasing time of digestion, but 8 hour-digestion regarding to putrefaction was suitable. 5. The most effective decolorization was successively passed on culumns of active carbon and anion exchanger (Dowex 2-x-8) at room temperature. In separate treatments, the effective order of decolorization was as follows; (Dowex 2-x-8)>Active carborn>Amberite IR-120 6. The powder type of the soy protein source obtained by concentration below $60^{\circ}C$ contains 12.51% of moisture, 66.31% of protein, 4.25% of fat, 12.75% of carbohydrate, 4.18% of ash.

• Food Science and Preservation
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• v.19 no.5
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• pp.744-750
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• 2012

In this study, the optimal conditions for phenolic-compound extraction from medicinal plants were found to be 24 h and about 50% ethanol. The electron-donating scavenging activities (DPPH), ABTS radical-cation decolorization (ABTS), antioxidant protection factor (PF), and thiobarbituric acid reaction substance (TBAR) were measured to determine the antioxidant activities of the extracts of Sanguisorba officinalis Linn., Citrus unshiu Markovich, Melia azedarach L., Asparagus cochinchinensis Merr., Citrus unshiu S., Polygonum aviculare L., and Leonurus sibiricus L. The total phenolic contents of the extracts of medical plants were determined to be 0.45-3.00 mg/g in the water extracts and 0.33-3.15 mg/g in the 50% ethanol extracts. The electron-donating abilities (EDA) of the water and ethanol extracts were both above 85% at the $50{\mu}g/ml$ concentration. The ABTS radical-cation decolorization was above 80% at the $100{\mu}g/ml$ concentration in all the extracts of various medicinal plants. The antioxidant protection factor (PF) of the Melia azedarach L. extracts was found to be $1.65{\pm}0.40$ PF in the water extracts at the $100{\mu}g/ml$ concentration, and was higher than those of the other medicinal-plant extracts. The TBAR inhibition rates of all the medicinal-plant extracts, except Asparagus cochinchinensis Merr., were above 85% at the $100{\mu}g/ml$ concentration. These results confirmed that the various oriental medicinal plants (Sanguisorba officinalis Linn., Citrus unshiu Markovich, Melia azedarach L., Asparagus cochinchinensis Merr., Citrus unshiu S., Polygonum aviculare L., and Leonurus sibiricus L.) that were included in this study are useful functional-food sources.

• Korean Journal of Plant Resources
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• v.27 no.1
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• pp.29-34
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• 2014

The aim of the study was to examine the cosmetic and biological activity of Lagerstroemia indica L. and it is possible that can be used as a cosmetic ingredient for application of cosmetic industries. Lagerstroemia indica L. branch was extracted with 70% acetone in water. In the result of DPPH (1,1-diphenyl-2-picryl-hydrazyl) scavenging radical activity, acetone extract of Lagerstroemia indica L. branch were higher than 73% at the 50 ppm concentration. ABTS radical cation decolorization activity by acetone extract were higher than 78% at the 50 ppm. Both examine of DPPH and ABTS showed high antioxidative activities at the 50 ppm. In the result of nitrite scavenging ability, acetone extract were higher than 63% at the 50 ppm. Collagenase inhibition activity by extract were higher than 85% at the 50 ppm. Extract is showed high collagenase inhibition more than comparison group EGCG at all concentration. These results suggest that Lagerstroemia indica L. has a great potential as a cosmeceutical raw material as well as anti-oxidant and anti-inflammatory and collagenase inhibition activity.

• Microbiology and Biotechnology Letters
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• v.47 no.2
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• pp.250-258
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• 2019

Peniphora sp. JS17, isolated from forest old tree, produced extracellular enzymes that decolorized human hair melanin. The JS17 strain had laccase and manganese peroxidase activity while it did not has lignin peroxidase activity. Batch culture indicated that the melanin decolorization activity of JS17 strain originated from laccase. The culture conditions to maximize the production of melanin bleaching enzymes from Peniophora sp. JS17 mycelia were investigated. Among the tested media for the laccase production, minimal medium (2% glucose, 0.2% malt extract, 0.1% $KH_2PO_4$, 0.4% $MgSO_4{\cdot}7H_2O$) showed the highest activity of laccase. Then, to optimize the culture condition for the laccase activity, the influence of various carbon and nitrogen sources was investigated in minimal medium. Among various carbon and nitrogen sources, 2% xylose and 0.4% tryptone showed the highest production of laccase, respectively. The enzyme was purified using $(NH_4)_2SO_4$ precipitation and Hitrap Q sepharose column, and the purified enzyme showed two isoenzymatic bands with molecular masses of about 70 kDa by SDS-PAGE. The melanin decolorization activity was 77% and 55% within 48 h in the presence of 1-hydroxybenzotriazole (HBT) and syringaldehyde, respectively, whereas only about 9% melanin decolorized in case of no mediator.

• Preventive Nutrition and Food Science
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• v.14 no.4
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• pp.358-361
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• 2009

Aldose reductase was purified to electrophoretic homogeneity from porcine liver. The purified enzyme was a monomer of 36 kDa. The enzyme was strongly inhibited by $Cu^{2+}\;and\;Mg^{2+}$ ions. Incubation of the enzyme with pyridoxal 5'-phosphate led to complete inhibition of enzymatic activity, suggesting that lysine residue is involved at or near the active site of the enzyme. The enzyme exhibited a broad substrate specificity. Furthermore, the enzyme was capable of decolorizing Alizarin, an anthraquinone dye.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.463-467
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• 2003

Fundamental experiments were carried out about color removal efficiency of dyeing wastewater using a $bioreactor-TiO_2/UV$ reactor system. Color removal efficiencies of the Reactive Black 5 in the system were found to bo more than 80% in changes of dilution rate 0.04 to $0.08hr^{-1}$. In this research, a new color removal system of dying wastewater will be proposed after more investigation of decolorization efficiencies about different dyes and condition.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.402-402
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• 2003

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.279-279
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• 2005

• Proceedings of the Membrane Society of Korea Conference
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• 2004.11a
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• pp.129-131
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• 2004

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.476-476
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• 2005