• Restorative Dentistry and Endodontics
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• v.1 no.1
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• pp.26-32
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• 1975

After a vital pulpotomy in dogs' teeth, the responses of the remaining pulp tissue under hydroxides (calcium hydroxide, magnesium hydroxide, aluminium hydroxide and zinc hydroxide) were studied histologically. The class V cavities were prepared on the teeth and the pulp was amputated. Each hydroxide was placed over the amputated tissue and the cavity was sealed with zinc oxide eugenol cement. Animals were sacrificed after 3 days, 1, 2, and 3 weeks following the operation. The teeth were decalcified, sectioned and stained with hematoxylin and eosin. Microscopic examination reveals as follows; 1. Calcium hydroxide: Inflammatory change was seen in the superficial portion of the remaining pulp tissue at the 3rd day and 1st week. The incompleted calicified material began to be deposited from the canal wall at the 2nd week, and the advanced calcified material was seen at the 3rd week. 2. Magnesium hydroxide: Severe inflammatory change was seen in the superficial portion of the remaining pulp from the 3rd day and the 1st week samples. Inflammatory change was decreased at the 2nd week and the slight calcified material was deposited from the root canal at the 3rd week. 3. Aluminium hydroxide: Severe inflammatory changes were seen in the remaining pulp tissue, the blood vessel was dilated, and the odontoblasts were destroyed at the 3rd day and 1st week. The fibrous degeneration spread to the apex at the 2nd week. There was no evidence of newly formed odontoblasts or deposition of calcified material underneath aluminium hydroxide. 4. Zinc hydroxide: The micrscopic picture was destructive. A thick necrotic layer was found under the amputated surface at the 3rd day and 1st week. Granulation tissue formation as well as chronic inflammatory changes extended to the apical area in the pulp tissue. Also there were no sign of odontoblastic formation or calcified material at the 2nd and 3rd week.

• Restorative Dentistry and Endodontics
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• v.21 no.2
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• pp.517-529
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• 1996

The purpose of this study was to evaluate the apical sealing ability of the Thermafil endodontic obturation technique and to compare it with lateral condensation technique. 42 straight canals from extracted human anterior teeth and 42 curved canals(> $25^{\circ}$) from maxillary and mandibular molar teeth were selected. And 80 of them were divided into four groups, 20 canals respectively. The teeth in prior two groups had straight canals and the other two groups had curved canals. The rest of four canals served as positive and negative controls. After resecting anatomical crowns, all canals were prepared using a standard step-back technique. Lateral condensation was used to obturate two groups, one group of straight ones the other curved. And Thermafil obturators were also used in the same two groups. Obturated teeth were infiltrated by India ink for a week, decalcified and cleared with 5% nitric acid and methyl salicylate. The apical leakage and the frequency of filled lateral and accessory canals were measured with stereomicroscope and also apical extrusion of sealer and gutta-percha and obturation time were checked and the data were analyzed statistically(one-way ANOVA, t-test, Chi-square test). The results were as follows : 1. There was no significant difference in the degree of dye penetration between Thermafil and lateral condensation groups(p>0.05). 2. Apical extrusion of sealer and gutta-percha occurred significantly more often with Thermafil obturators in straight canals(p<0.05), but not significantly different in curved canals(p>0.05). 3. Canal obturation time with Thermafil obturators was significantly faster than lateral condensation (p<0.05). 4. The Thermafil groups showed a higher frequency of filled lateral and accessory canals than in the lateral condensation groups. But the difference was not statistically significant (p>0.05).

• Restorative Dentistry and Endodontics
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• v.10 no.1
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• pp.93-101
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• 1984

The experimental study was made to investigate the effect of the "Heliosit" composite resin on the dental pulp. The 36 class V cavities were prepared on the healthy permanent teeth of 3 days, and were divided into 5 groups and filled with the experimental filling materials. Control group: Zinc Oxide-Eugenol cement filling Experimental groups: Group 1: Dentin Adhesit application & Heliosit filling with or without dycal base Group 2: Heliosit filling with or without dycal base Group 3: Durafill filling with dycal base Group 4: Hipol filling with dycal base Animals were sacrificed after 1 weeks, 2 weeks, and 4 weeks following operation. The teeth were decalcified, sectioned and stained with hematoxylin and eosin. The results obtained form this study were as follows: 1. All experimental group showed slight pulp response. 2. Dentin Adhesit group showed minimal pulp response in both dycal bases and no base cases. 3. In group 2, mild pulp response was found in early stage and repairing process was found as the time elapsed. In no base cases, healing process was delayed slightly. 4. There was little difference in the result among Heliosit group, Durafill group and Hipol group.

• Restorative Dentistry and Endodontics
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• v.8 no.1
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• pp.139-145
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• 1982

Eighty four human maxillary second premolars were injected with china. ink, decalcified, cleared and used in study, in vitro, to determine the number of root canals, the frequency and location of lateral canals, the frequency and location of transverse ansatomoses, the curvature of root canals, the location of apical foramens, and the frequency of apical deltas. The results were as follows: 1. Most of the teeth demonstrated one canals, but the only tooth was found to have two canal. 2. Of the 101 canals studied, 85.1% of the canals were found to have lateral canals and these ramifications were usually located in the apical third of the root. 3. Of the two root canals in one root, 25.4% of the canals were found to have transverse anastomoses and were usually located in the middle third of the root 4. 33.3% of apical foramens were located directly on the root apex and 66.7% of them laterally, and 46.5% of canals showed distal curvature.

• The Journal of Korean Academy of Prosthodontics
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• v.15 no.1
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• pp.34-42
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• 1977

The author attempted to observed the histological changes of the temporomandibular joints of rabbits by including malocclusion. Thirth-two healthy male rabbits were devided into two groups; control and experimental group. Eight rabbits were kept as control group, while metal crowns were seated on unilateral lower molar teeth of twenty-four rabbits as experimental group. And the interocclusal distance of the incisal edge was kept 1.5mm from the begining to the end of the experimental periods. Rabbits of each group, one of control group and three of the experimental gorup, were killed at the intervals of one day, three days, one week, two weeks, four weeks, six weeks, eight weeks, and twelve weeks after experiment. The temporomandibular joint including condyle head, articular disc and glenoid fossa were excised and decalcified. The decalcified sections were made histologic sections. The results obtained were as follows. 1. The regressive changes of the condylar head were the main reaction in this experiment that consist of decreasing or increasing thickness of the fibrocartilage zone with hyaline degeneration, decreasing of the cellularity of the proliferative zone, and the irregularity of the arrangement of chondrocytes and size of the lacunae of cartilage cells with chondroclasia and osteoclasia in hypertrophic zone. 2. The regressive changes of the condylar surface of the crown seated side were persisted to the end of the experiment. 3. On the non-crown seated side, severe aggressive changes occurred in initial stage, but hyperplastic changes of the condylar surface noted in the middle of the experimental periods. 4. Although aggressive changes occurred in initial stage of experiment on the non-crown seated side, hyperplastic changes of the condylar surface were noted in the middle of the experimental periods, and remodeling appeared at the termination of the experimental periods. 5. The articular disc exhibited pannus formation on both crown seated and non crown seated side from the beginning of the experiment. The pannus persisted throughout the experiment on the crown seated side, but on the non-crown seated side it disappeared from six week group.

• Restorative Dentistry and Endodontics
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• v.14 no.2
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• pp.77-97
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• 1989

The purpose of this study was to observe the effect of ${\beta}GP$ in the remaining dental pulp tissue after pulpotomy in the dogs' teeth. For vital pulpotomy, 72 dogs' teeth were used and class V cavities were prepared and the pulps were amputated. ZOE and Dycal (Caulk Co., USA) were placed over the amputated tissue and cavities were sealed with ZOE cement in the control group. In the experimental group, ${\beta}GP$, ${\beta}GP$-ZOE, ${\beta}GP$-Dycal were placed over the exposed pulp tissues respectively. Dogs were sacrificed after 1, 2 and 4 weeks following the operations and the teeth were decalcified in the nitric acid, sectioned and stained with HE for light microscopic examination. For electron microscopic examination, specimens were made after 2 and 4 weeks following the operation. A comparative microscopic examination revealed as follows. 1. The dentin bridge was formed continuously due to osteodentin in the ${\beta}GP$-Dycal group at the 2nd week, the dentin bridge composed of osteodentin and tubular dentin was observed at the 4th week. 2. Osteodentin formation was vigorously in the ${\beta}GP$-Dycal than in the Dycal group. 3. In the surface of osteodentin the osteodentinoblasts showing vivid synthetic activity were observed and the matrix vesicles were presented during calcification of osteoid dentin matrix. 4. The dentin bridge formation was not observed in ${\beta}GP$ group and ${\beta}GP$-ZOE group.

• Restorative Dentistry and Endodontics
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• v.24 no.3
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• pp.482-489
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• 1999

The purpose of this study was to compare sealing ability between lateral condensation and continuous wave of condensation in depend of root canal curvature. In this study, we divided fifty-six human molar teeth into two group in depend of Schneider method, and then subdivided them into four experimental group (each group is composed of twelve teeth) by canal curvature and obturation method, and eight teeth were served as positive and negative controls. Specimens were prepared by Quantac 2000 series file and obturated by lateral condensation or System B. Specimens were immersed in india ink for 7 days, decalcified by 10% nitric acid, dehydrated by 75, 95 and 100% alcohol in order, cleared by methyl salicylate and then measured of dye penetration with stereomicroscope(${\times}6.5$ magnification) and Image Pro plus. The data were analyzed stastically by one-way ANOVA and Scheffe test. The data were as follows: 1. The mean leakage was $0.725{\pm}1.167$ for group A, $0.813{\pm}0.921$ for group B, $0.809{\pm}0.997$ for group C, $1.111{\pm}1.147$ for group D, but no significant difference among them(p>0.05). 2. Lateral condensation had better sealing ability than continuous wave of condensation, but no significant difference among them(p>0.05). 3. There was no significant difference between root canal curvature degree and microleakage(p>0.05).

• The Journal of Korean Academy of Prosthodontics
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• v.16 no.1
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• pp.7-11
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• 1978

The author attempted to observe the histological changes of the periodontal structures induced by trauma from occlusion. Eighteen healthy rabbits were devided into two groups; control and experimental group. Three rabbits were kept as control group, while metal crowns were seated on unilateral lower molar teeth of fifteen rabbits were kept as experimental group. And the interocc1usal distance of the incisal edge was kept 1.5mm from begining to the end of the experimental period. Rabbits of each group consisting with three rabbits were killed at the intervals of three days, one week, two weeks, four weeks, eight weeks. The antagonistic teeth of maxilla including periodontal teeth were excised and decalcified for histologic preparation. The results obtained were as follows. 1. Destructions and ulcer formation of the sulcular epithelium and gingival epithelium occurred and persisted from the beginning of the experiment to the four weeks after experiment. The epithelal attachment were proliferated apically. 2. The pressure site were observed at the apical protion, where they showed compression of the periodontal ligament, thrombosis and congestion of blood vessels and hemorrhages. 3. At the pressure site, there appeared osteoclasts and bone resorption from the first week of experiment and it became more prominent at the second week with the extend into the marrow spaces adjacent to the periodontal membrane. 4. The phenomenon of bone apposition and resorption occurred at the fourth week of experiment. The reverse line of bone trabecular were more prominent. And the reactions were ceased at the eighth week of experiment.

• Journal of Periodontal and Implant Science
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• v.23 no.3
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• pp.374-390
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• 1993

Regeneration of periodontal tissue after a loss of attachment due to disease or trauma repesents an important issue in dentistry, and various bone graft materials have been used to regenerated lost periodontal tissue and restore proper fuctions. Among those, allografts have been extensively researched and widely used clinically, since they are known to possess an excellent osteoinduction capability and result in proper topography of alveolar bone. Regeneration of periodontal tissue in supraalveolar defects may be technically difficult. However, a large amount of regeneration has been observed by complete tissue coverage of involved teeth. In this study, supraalveolar defects in adult dogs were treated with periodontal surgery, decalcified freez-dried bone allograft, complete tissue coverage was attained, and effects on repair and regeneration of alveolar bone, cementum and periodontal ligament were studied. Exposure of premolar furcation of adult dogs was attained by removing marginal alveolar bone down to 5mm from CEJ, and root surfaces were planed with curettes. On the left side, defects were treated without any allograft(Control Group). On the right side, a DFDB was used(Experimental Group). In all groups, flaps were coronally positioned and sutured, completely submerging the treated defects. At two weeks, the crown were exposed 2-3mm. Healing progresses were histologically observed after eight weeks and the results were as follows : 1. Distance from CEJ to AJE was : $2.82{\pm}0.66mm$ in the control group, $1.71{\pm}0.51mm$ in experimental group, with significant differences between groups.(P<0.01) 2. Periodontal repair was : $2.18{\pm}0.66mm$ in the control group, $3.29{\pm}0.51mm$ in experimental group, with significant differences between groups.(P<0.01) 3. Connective tissue repair was : $1.43{\pm}0.52mm$ in the control group, $0.76{\pm}0.47mm$ in experimental group, with significant differences between groups.(P<0.01) Orientation of connective tissue fibers in relation to root surfaces was : mostly parallel in the control group, vertical or parallel or irregular in experimental group. 4. The amount of cementum formation was : $1.66{\pm}0.58mm$ in the control group, $2.86{\pm}0.66mm$ in experimental group, with significant differences between groups. 5. The amount of alveolar bone formation was : $0.76{\pm}0.72mm$ in the control group, $2.53{\pm}0.56mm$ in experimental group, with significant differences between groups.(P<0.01)

• Restorative Dentistry and Endodontics
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• v.26 no.4
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• pp.307-315
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• 2001

The application of Nd:YAG laser and irrigants to the root surface can change its surface configurations. The purpose of this study was to investigate the effects of Nd:YAG laser and irrigants on the apical seal of obturated canals. In this study, 66 single rooted teeth were randomly assigned to 4 group of 14 teeth each. 8 teeth were served us positive and negative controls. The teeth were divided into 6 groups as follows. Group A: Nd:YAG laser, 5% NaOCl + Rc-prep Group B: Nd:YAG laser, Saline Group C: 5% NaOCl + Rc-prep Group D: Saline Group E: Positive control Group F: Negative control 66 teeth were instrumented using Maillefer ProFile$^{\circledR}$ (Orifice Shapers, .04 taper, .06 taper Dentsply, Switzerland). Two of each group were selected at random, and the canal wall surfaces were examined under a SEM. 12 teeth of each group were obturated using by lateral condensation technique. Specimens were immersed in india ink for 7days, decalcified by 10% nitric acid, dehydrated by 75. 80. 85, 90, 95 and 100% alcohol in order cleared by methyl salicylate and then measured of dye penetration with stereomicroscope($\times$15 magnification) and Image Pro plus. The data were analyzed statistically by one-way ANOVA test and Duncan's Multiple Range test. The results were as follows : 1. The mean leakage was 0.128$\pm$0.376 for group A, 0.237$\pm$0.325 for group B, 0.397$\pm$0.468 for group C, 0.586$\pm$0.402 for group D, and there were statistically significant differences between group A and group D, group B and group D. (p<0.05). 2. Group A had better sealing ability than Group C, but there was statistically no significant differences. (p>0.05). 3. Group B had better sealing ability than Group D and there was statistically significant difference. (p<0.05). 4 Group A had better sealing ability than Group B, but there was statistically no significant difference. (p>0.05). 5. Group C had better sealing ability than Group D, but there was statistically no significant difference. (p>0.05). 6. As a result of observation under SEM, Smear layers were removed in Group A, B. but Smear layers were partially removed and smear plugs were remained in Group C, Smear layers were not removed in Group D. To be specially, Melting of smear layer were showed in Group C. 7. These results suggests that the laser has a potential in reducing the apical microleakage of obturated canals.