• Korean Journal of Veterinary Research
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• v.44 no.4
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• pp.593-605
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• 2004

An egg has been considered as one of the most important food sources because of it's nutritional superiority and reasonable price. With the complexity of egg flow system from production to consumption in Korea, preventive measures for egg safety have been required. Therefore, our study was carried out to analyze hazards for the egg under farm level and develope preventive measures with a purpose of obtaining egg safety. To analyze biological hazards, microbial contamination of egg(normal, dirty and cracked), water, feed, manure and equipments associated with laying were investigated. One isolate of Salmonella enteritidis and S. bardo were detected from the manure and dirty egg shell respectively. The sanitary conditions in the farm were surveyed by questionaries. Confirmation of vaccination for purchasing chicks and establishment of sanitary guidances for water and type of water suppliers ought to be preceded. Feed supplier systems including feed tanks, feed pipes and hoppers were known that their contamination might give it a chance to infect individuals and egg content and shell. The safe and rapid disposal of dead chickens and rodent were reported as more crucial factors to prevent infectious disease and manage good sanitation. Egg selector and collecting belt should be kept properly not to be contaminated from egg fluids and feces. It should be also considered that regular gathering of eggs, removal of dirty or cracked ones, storage under refrigeration and the use of disposable egg tray were continuously fulfilled. Conclusively, Our results suggested that HACCP-based system for providing fresh and safe eggs to consumers should be applied to the farm.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.3
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• pp.14-18
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• 2023

White leg shrimps cultured in an inland private aquaculture farm with low salinity waters showed abnormal swimming behavior and appetite reduction in July 2022. Then, gradual mortality was observed in the aquaculture farm. During the diagnosis, bacterial strain KNUAF-SHP3 was isolated from the hepatopancreas of the dead shrimps. Based on the sequence of 16S rRNA gene, KNUAF-SHP3 was proved to be Shewanella sp., clustering into a group with S. algae MARS 14 and S. chilikensis JC5T. According to the result of experimental infection test, all shrimps challenged with high concentrations, 2.1×108 CFU/ml and 2.1×109 CFU/ml showed apparent disease symptoms and the cumulative mortality rates reached 100% in 7 days post challenge. These results emphasized that Shewanella isolate in this study can be a potential pathogen of white leg shrimp cultured in low salinity water.

• Advances in materials Research
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• v.13 no.3
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• pp.243-251
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• 2024

The asymmetric polylactic acid (PLA) membrane was prepared via phase inversion method using non-solvent induced separation (NIPS) technique. This study aims to synthesized as well as to characterize the PLA membrane and evaluating the membrane performance on water flux and permeability. In addition, this research also studied the removal performance of methylene blue dye. The polymer solution has been prepared using 12 wt.% of PLA and dissolved in 88 wt.% of Dimethylacetamide (DMAc) as a solvent. Then, the cast film was immersed in different ratio of coagulant bath medium (distilled water: methanol: ethanol) ranging from 100:0:0, 75:25:0, 75:0:25 and 75:12.5:12.5, respectively). Several characterizations were performed which include, membrane contact angle and membrane porosity. Performance PLA membranes were determined in terms of water flux and permeability at 1 bar transmembrane pressure using dead-end permeation cell. Finally, methylene blue (MB) removal efficiency was tested at the same transmembrane pressure. The findings revealed that the increase of alcohol concentration in coagulant bath resulted in higher porosity and lower contact angle. In short, MB dye rejection efficiency is also closely related to the amount of alcohol ratio used in coagulant baths. Increases in concentration of methanol and ethanol in coagulant bath medium increases the membrane porosity thus increased in efficiency of methylene blue rejection.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.7
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• pp.525-531
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• 2013

In an effort to find a solution to the eutrophication of major Korean rivers, a method to utilize multi-purpose filtration pond was investigated. As literature showed that oyster shell is known to be the most adequate for the removal of dissolved phosphorus in Korean rivers, batch and column experiments were performed using oyster shell as an adsorbent in this study. The results of the batch experiment showed that the removal of dissolved phosphorus from river water through adsorption as a way of preventing algal growth was not practical. The results obtained from the column experiment, however, suggested that oyster shell may be utilized as an adsorbent under limited conditions. Based on the results of the experiments a methodology was proposed to remove algae from river water through the use of multi-purpose filtration pond. This method involves mechanically removing the accumulated algae cake from the surface of the artificial stream in the pond towards the condensing part located at the lower reach of the stream, where particles gather before the final removal. In addition, employment of oyster shell as an adsorbent in the condensing part allows prevention of phosphorus released from the dead algae re-entering the river water.

• The Korean Journal of Pesticide Science
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• v.3 no.1
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• pp.57-65
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• 1999

The midge Chironomus riparius is a member of widespread dipteran insect which has been used for sediment toxicity test. The 2nd-3rd larvae Chironomus riparius were exposed to $^{14}C$-lambda-cyhalothrin in laboratory water only system(A) and sediment-water system(B) at $20{\pm}2^{\circ}C$. The concentration was nominally in the range of 0.16 to $20{\mu}g/{\ell}$ and 62.5 to 8000 ${\mu}g/kg$, respectively. System(A) was prepared by applying the chemical to 250 ml water and introducing Chironomus riparius larvae into the system(day 0). System(B) was prepared by applying the chemical to the 25 g of soil and 250 ml water and shaking and rolling them for two hours. After allowing the system to settle for two days, Chironomus riparius larvae were introduced into the system. After 72 hours, the two systems were terminated and dead larvae were observed. The measurement of residue in water was conducted at the start(day 0) and the end of the study(day 3). The residue of $^{14}C$-lambda-cyhalothrin in water was accounted for approximately 65% of nominal concentration in system(A) and $0.2{\sim}0.4%$ in system) on 3 days. $LC_{50}$ was less than $0.156{\mu}g/{\ell}$ in system(A) and 889 ${\mu}g/kg$ in system(B), based on the nominally applied concentration.

• Geomechanics and Engineering
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• v.8 no.6
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• pp.859-872
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• 2015

Pebble matrix filtration (PMF) is a water treatment technology that can remove suspended solids in highly turbid surface water during heavy storms. PMF typically uses sand and natural pebbles as filter media. Hand-made clay pebbles (balls) can be used as alternatives to natural pebbles in PMF treatment plants, where natural pebbles are not readily available. Since the high turbidity is a seasonal problem that occurs during heavy rains, the use of newly developed composite clay balls instead of pure clay balls have the advantage of removing other pollutants such as natural organic matter (NOM) during other times. Only the strength properties of composite clay balls are described here as the pollutant removal is beyond the scope of this paper. These new composite clay balls must be able to withstand dead and live loads under dry and saturated conditions in a filter assembly. Absence of a standard ball preparation process and expected strength properties of composite clay balls were the main reasons behind the present study. Five different raw materials from industry wastes: Red Mud (RM), Water Treatment Alum Sludge (S), Shredded Paper (SP), Saw Dust (SD), and Sugar Mulch (SM) were added to common clay brick mix (BM) in different proportions. In an effort to minimize costs, in this study clay balls were fired to $1100^{\circ}C$ at a local brick factory together with their bricks. A comprehensive experimental program was performed to evaluate crushing strength of composite hand-made clay balls, using uniaxial compression test to establish the best material combination on the basis of strength properties for designing sustainable filter media for water treatment plants. Performance at both construction and operating stages were considered by analyzing both strength properties under fully dry conditions and strength degradation after saturation in a water bath. The BM-75% as the main component produced optimum combination in terms of workability and strength. With the material combination of BM-75% and additives-25%, the use of Red Mud and water treatment sludge as additives produced the highest and lowest strength of composite clay balls, with a failure load of 5.4 kN and 1.4 kN respectively. However, this lower value of 1.4 kN is much higher than the effective load on each clay ball of 0.04 kN in a typical filter assembly (safety factor of 35), therefore, can still be used as a suitable filter material for enhanced pollutant removal.

• 한국해양학회지
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• v.22 no.3
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• pp.207-215
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• 1987

The Keum River Estuary was investigated two times in April and July, 1986, to study process controlling chlorophyll distribution in estuarine waters. During the surveys, distribution patterns were studied for chlorophyll-a, nutrients, ph, SPM (Suspended Particulate Matter), DO (Dissolved Oxygen), temperature, salinity, etc. During April survey(low-discharge period), sea water penetrated to Kangkyung, about 35km upstream from the constructing weir, while in July (high-discharge period) only to 3 km upstream from the weir,In April SPM showed very high concentrations (500mg/l)on the average. But very low concentrations(about10mg/l)were observed in July due to high discharge of fresh water.Chlorophyll-a concentrations showed large variations both in time and space :much higher concentraations in July than in April and sharp decrease in concentrations at the fresh water-sea water interface (April:$6.5\mu\textrm{g}/{\ell}$ for fresh waters and 41.4\mu\textrm{g}/{\ell}$ forestuarine waters). Differebce ub chlorophyll-a concentrations for these two surveys appear to be caused mainly by the difference in effectiveness of penetrating lights controlled by SPM in the waters. Sharp decrease in chlorophll-a at the fresh water-sea water interface is believed to be resulted from mass mortality of fresh water phytoplankton caused by changes in osmotic pressure in the region. Observations in the same regions such as increase in AOU(Apparent Oxygen Utilization)and ammonia, decrease in PH,probably resulted through decomposition processes of dead planktons,furtuer support the idea.

• Economic and Environmental Geology
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• v.43 no.6
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• pp.555-564
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• 2010

Remediation process by using the bio-carrier (beads) with dead Bacillus sp. B1 and polysulfone was investigated for heavy metal contaminated groundwater. Sorption batch experiments using the bio-carrier were performed to quantify the heavy metal removal efficiencies from the contaminated solution. The analyses using SEM/EDS and TEM for the structure and the characteristic of precipitates on/inside the beads were also conducted to understand the sorption mechanism by the bio-carrier. Various amounts of freeze-dried dead Bacillus sp. B1 were mixed with polysulfone + DMF(N,N-dimethylformamide) solution to produce the bio-carrier (beads; less than 2mm in diameter) and 5% of Bacillus sp. B1 in the bio-carrier was optimal for Pb removal in the solution. The removal efficiency ratings of the bio-carrier for Pb, Cu and Cd were greater than 80% after adding 2g of bio-carrier in 50ml of aqueous solution (<10mg/L of each heavy metal concentration). Reaction time of the bio-carrier was very fast and most of the sorption reaction for heavy metals were completed within few hours. Batch experiments were duplicated at various pH conditions of aqueous solutions and Cu and Pb removal efficiencies highly maintained at wide pH ranges (pH 2-12), suggesting that the bio-carrier can be useful to clean up the acidic waste water such as AMD. From SEM/EDS and TEM analyses, it was observed that the bio-carrier was spherical shape and was overlapped by many porous layers. During the sorption experiment, Pb was crystallized on the surface of porous layers and also was mainly concentrated at the boundary of Bacillus sp. B1 stroma and polysulfone substrate, showing that the main mechanism of the bio-carrier to remove heavy metals is the sorption on/inside of the bio-carriers and the bio-carriers are excellent biosorbents for the removal of heavy metal ions from groundwater.

• Journal of Wetlands Research
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• v.20 no.4
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• pp.353-362
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• 2018

The purpose of this study is to suggest a structure plan for improving the utilization of inactive storage in the dam for overcoming the drought. Inactive storage in the dam is composed of the emergency storage and dead storage. The emergency storage can be used for the case of emergency such as drought. But, in general, the dead storage for sedimentation is not used even for the emergency. Therefore, this study considers the part of dead storage that the sedimentation is not progressed yet can be used during the severe drought period and is called "drought storage in a dam". The accurate Sediment Level(SL) analysis for the computation of the drought storage should be performed and so the present and future SL in the dam reservoir is estimated using SED-2D linked with RMA-2 model of SMS. After the consideration of additionally available storage capacity based on the estimated SL, the drought storage is finally determined. Present data based on historical data, future predicted future climate factors by Representative Concentrarion Pathways(RCP) 8.5 scenario. Then, using the TANK model, dam inflows were determined, and future period such as SL and drought storage were suggested. As the results, we have found that the available drought storage will be reduced in the future when we compare the present drought storage with the future one. This is due to a increase variability of climate change. Therefore, we should take the necessary study for the increase of available drought storage in the future.

• Journal of fish pathology
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• v.21 no.1
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• pp.35-43
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• 2008

Pathological symptoms and hematological parameters of red sea bream, Pagrus major and water temperature in the culture ground was investigated to clarlify the cause of winter moratility. Dead fish showed green liver and accumulation of ascites in the cavity. A few Bivagina tai were also found on the gill but either bacteria or virus were not. When hematological parameters from fish taken before/after the winter mortality were compared, blood glucose, serum AST and ALT, and total cholesterol, trigyceride and total protein were significantly decreased in the fish after the winter mortality. These results may explain that the nutritional level of fish was decreased because fish could not fed during winter season. According to the CORI monitoring system operating by KODC, NFRDI a long term water temperature from Dec. 25, 2005 to Feb. 24, 2006 (60 days) were exposed the low water temperature environments to the red sea bream. First of all, mass mortality began at Sarayngdo where low temperature below 8℃ continued for 42 days. The winter mortality did not occured in the depths of 9 m to 19.2 m where difference of water temperature in the surface and bottom was only within 0.1℃. But in the depth of 7.5 m to 11 m winter mortality occured where water temperature in the surface and bottom showed much variation ranged from 0.2℃ to 1.4℃. From these results, great difference of water temperature in the surface and bottom of the culturing area might results in winter mortality of red sea bream.