• Title/Summary/Keyword: Days to flower

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In Vitro Production of Indian Citrs Ringspot Virus-Free Plants of Kinnow Mandarin (Citrus nobilis Lour X C. deliciosa Tenora) by Ovule Culture

  • Singh B.;Sharma S.;Rani G.;Zaidi A.A.;Hallan V.;Nagpal A.;Virk G.S.
    • Journal of Plant Biotechnology
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    • v.7 no.4
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    • pp.259-265
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    • 2005
  • Indian citrus ringspot virus (ICRSV)-free plants of Kinnow mandarin (Citrus nobilis Lour x C. deliciosa Tenora) were raised from virus-infected plants using unfertilised ovules as explants. Plants were tested by indirect ELISA and RT-PCR before using their explant. An amplified product of 539 bp was obtained by RT- PCR in ICRSV infected plants. Unfertilized ovules were excised from unopened flower buds of plants tested postive for virus and were cultured on Murashige and Skoog's (MS) basal medium supplemented with various concentrations of kinetin (KN) or malt extract (ME). Maximum induction (31.94%) of embryogenic callus was observed on MS medium supplemented with KN ($9.29\;{\mu}M$). Transfer of embryogenic calli to similar media composition resulted in somatic embryogenesis in all cultures, with an average number of 60.36 globular, 17.39 heart and 7.71 cotyledonary-shaped somatic embryos per culture. All cotyledonary shaped embryos developed into complete plantlets within 60 days on transfer to similar medium. Embryogenic callus induction, somatic embryo formation, maturation, germination and plantlet formation were achieved on MS medium supplemented with KN ($9.29\;{\mu}M$) alone. The plantlets derived from somatic embryos were transferred to sterilized soil, sand and vermiculite (3:1:1) mixture. After acclimatization, the plantlets were transferred to screen house and were indexed for ICRSV employing indirect ELISA and RT-PCR and found free of virus. A distinct feature of this study is the induction of somatic embryogenesis from unfertilised ovules to produce virus-free plants.

Morphological Variation of Two Cultivated Types of Perilla Crop from Different Areas of China

  • Ma, Shi Jun;Lee, Ju Kyong
    • Horticultural Science & Technology
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    • v.35 no.4
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    • pp.510-522
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    • 2017
  • To better understand the morphological variation for Perilla crop in different areas of China, we studied the morphological variation in 87 accessions (84 cultivated var. frutescens and three cultivated var. crispa) from high latitude (Northeast China) and middle latitude (North and Northwest China) areas of China by examining seven quantitative and 10 qualitative characters. Analysis of the morphological variation determined that there was significant morphological differences in five quantitative traits between cultivated var. frutescens and cultivated var. crispa, including effective number of branches (QN2), number of internodes (QN3), number of branches (QN4), length of the largest inflorescence (QN5), and days from germination to flowering (QN7). However, two quantitative traits-plant height and number of florets of the largest inflorescence-did not show any significant differences between cultivated var. frutescens and cultivated var. crispa. In addition, significant differences for six quantitative traits were found between the accessions of cultivated var. frutescens originating from high and middle latitude areas in China, which included QN2, QN3, QN5, number of florets of the largest inflorescence (QN6), and QN7. Principal components analysis (PCA) identified five quantitative characters [plant height (QN1), QN2, QN3, QN4, QN7] and six qualitative characters [fragrance of plant (QL1), color of reverse side of leaf (QL3), degree of pubescence (QL5), color of flower (QL6), shape of leaf (QL7), and hardness of seed (QL10)] that contributed to the positive direction on the first axis. The other quantitative and qualitative characters contributed to the negative direction on the first axis. Most accessions of cultivated var. frutescens and cultivated var. crispa were clearly separated by the first axis. In addition, most accessions of cultivated var. frutescens are from high latitude and middle latitude areas that were clearly separated by the first axis, except for several accessions. The findings from this study will provide useful information towards understanding the morphological variation of Perilla crop according to geographical distribution in high and middle latitude regions of China.

A Study on the Costume of the Gods in the Ancient Roman Myths (고대 로마 신화에 등장한 신들의 복식 연구)

  • 임상임;추미경
    • Journal of the Korean Society of Clothing and Textiles
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    • v.25 no.5
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    • pp.945-956
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    • 2001
  • The costume for gods in the Ancient Roman myth was studied by examining in the embossed-carving, sculptures, mural-painting and various documents of the period. The clothes item, hairstyle, symbol & ornaments and shoes of the gods in the Roman myths were analyzed to understand the formative process in the religious costumes, and results are as follows. First, the costumes for male gods are the same as those for ordinary men lived in ages, including toga, paludamentum, tebenna, tunica. The costumes for female gods are also the same as those for ordinary women lived in ages, including stola, palla, tunica. Second, the hairstyle of male gods is short curled-hair, twisty roll-up hair and braid long-hair due to the influence of Etrurian in the early days. Another hairstyle is appeared in form of the laurelled-crown. The hairstyle of female gods is curled long-hair, chignon style and braid roll-up hair decorated with hairband. Third, the symbol & ornaments which represent the responsible possession of male gods, are appeared in beard, a stick of the message, arrow-case, offering-dish, antlered-glass, shield, spear-handle, as the superhuman forms, such as the lightning shape. For female gods they are is appeared plastron, a stick of the message, flower. some female gods wear necklace, foot-ring, bracelet. Fourth, male gods wear shoes in forms of sandal, buskin, boots, but all female gods are in barefoot. It could be explained by the fact that a man-centered society in the ancient roman is reflected in the world of gods. As mentioned, Gods costume in Roman myths was very similar to or nothing different from the ordinary peoples in Rome. However, they used corps, sacrifices or sepcific symbols depending on the fields that they were responsible for in order to be distinguished from the ordinary people. This is the result from the peoples expression that they should keep a close relationship with Gods hoping to be protected, and rely on them under whatever circumstances.

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Effects of Shading, Chilling and Gibberellin Treatments on the Flowering of Azalea (Rhododendron spp.) (차광, 저온 및 지베렐린 처리가 Azalea의 개화에 미치는 영향)

  • Chung, Hae-Joon;Yoo, Bong-Sik
    • The Journal of Natural Sciences
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    • v.10 no.1
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    • pp.63-71
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    • 1998
  • This research was conducted to control flowering of azalea (Rhododendron spp.) by shading, chilling and gibberellin treatments. Higher shading among treatments resulted in extended duration of flowering (DF) and increased number of flowers (NF) in all varieties tested. Number of days to flower (NDF) was increased in 'Ripple' by shading treatment, but this was not affected in other was vice verse in 'Reinhold Ambrosia' resulting in decreased NDF. Chilling treatment followed by high shading increased NDF and shortened DF in 'Inga' and 'Ripple', but that extended NDF and DF and increased NF in 'Reinhold Ambrosia'. In flowering response to $GA_3$ resulted in decreased NDF and increased NF. These results indicated that treatments of shading, chilling and gibberellin to azalea extended DF and increased NF, but NDF appeared different according to varieties.

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Selection of Culture Media Applied to Grafted Cactus 'Hwangwall' for Export (수출용 접목 선인장 '황월'에 적합한 배지선발)

  • Kim, Yang Hee;Ryu, Byung Yeal
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.3
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    • pp.171-178
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    • 2010
  • This work is for selecting superior media which is similar to Peat Moss that is in general use as media of Gymnocalycium mihanovichii for Export such as 'Hwang wall' but lower price. The result on growth of 7 kinds of media (Peat Moss, BM6 Culture Medium, Coco Peat, Hydro Cray, Hydro Ball, Hugato, Vermiculite) which are applied watering (overhead irrigation, sub irrigation) based on bichemical analysis including chemical, physical analysis are following. The result of bi-chemical analysis shows that Coco Peat was stabilized planting after 90 days and Hydro Ball has high water holding capacity. The experimental result of growth in grafted cactus 'Hwangwall' shows Coco Peat is similar to Peat Moss on organic matter and in case of inorganic media, Hugato, Vermiculite, Hydro Cray made satisfactory results. But, the weight of inorganic media is too light to be tied. Consequently, Coco Peat and sphagnum moss as organic media has lower price and the condition of growth is analogous to Peat Moss. On the other hands, Hydro Ball was chosen as a substitute of Peat Moss in inorganic media.

Effect of Chamomile Flower Extract on Septic Arthritis due to Candida albicans (카모마일 꽃 추출물의 Candida albicans 기인성 감염성 관절염에 대한 효과)

  • Kim, Jeonghyeon;Kim, Songyi;Hong, Yuna;Kim, Yeong Shik;Han, Yongmoon
    • YAKHAK HOEJI
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    • v.58 no.5
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    • pp.343-348
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    • 2014
  • In the present studies, we examined effect of chamomile flowers extract (CH-Ex), which has traditionally been used as antiphlogistics in Europe for many centuries, against Candida albicans-caused septic arthritis. Candida albicans is a major etiological agent among fungal septic arthritis. This effect was investigated in a murine model of the septic arthritis. That is, mice that were given an emulsion form of C. albicans cell wall (CACW) via footpad route were treated intraperitoneally with the CH-Ex for 3 times every 3 days. Degrees of the footpad-swellings were measured with dial gauger. Data showed that the CH-Ex resulted in the reduction of swelling. For instance, at Day 9 when swelling reached the highest peak, there was up to app. 60% reduction of edema in mice injected with the CH-Ex, compared to that of the control mice that received no treatment (P<0.05). This therapeutic anti-arthritic activity appeared to be mediated by inhibitions of NO (nitric oxide) production from activated RAW264.7 macrophages and proliferation of Con A-treated T lymphocytes. Analysis by HPLC revealed that the CH-Ex contained eight polyphenolic compounds including chlorogenic acid (CRA) and rutin. We have reported the CRA and rutin respectively have the anti-arthritic activity. This correlation implicates that CRA and rutin in the CH-Ex may be responsible for the activity. Combined all together, the CH-Ex has anti-arthritic activity against C. albicans-caused septic arthritis, possibly by inhibiting NO production and proliferation of T cells. This activity seems to be contributed by, at least, CRA and rutin among the compounds in the CH-Ex.

Studies on the Construction of Mutant Diversity Pool (MDP) lines, and their Genomic Characterization in Soybean

  • Dong-Gun Kim;Sang Hoon Kim;Chang-Hyu Bae;Soon-Jae Kwon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.9-9
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    • 2021
  • Mutation breeding is useful for improving agronomic characteristics of various crops. In this study, we constructed soybean Mutant Diversity Pool (MDP) from 1,695 gamma-irradiated mutants through two selection phases over M1 to M12 generations; we selected 523 mutant lines exhibiting at least 30% superior agricultural characteristics, and, second, we eliminated redundant morphological phenotypes in the M12 generation. Finally, we constructed 208 MDP lines and investigated 11 agronomic traits. We then assessed the genetic diversity and inter-relationships of these MDP lines using target region amplification polymorphism (TRAP) markers. Among the different TRAP primer combinations, polymorphism levels and PIC values averaged 59.71% and 0.15, respectively. Dendrogram and population structure analyses divided the MDP lines into four major groups. According to an analysis of AMOVA, the percentage of inter-population variation among mutants was 11.320 (20.6%), whereas mutant inter-population variation ranged from 0.231 (0.4%) to 14.324 (26.1%). Overall, the genetic similarity of each cultivar and its mutants were higher than within other mutant populations. In an analysis of the genome-wide association study (GWAS) using based on the genotyping-by-sequencing (GBS), we detected 66 SNPs located on 13 different chromosomes were found to be highly associated with four agronomic traits: days of flowering (33 SNPs), flower color (16 SNPs), node number (6 SNPs), and seed coat color (11 SNPs). These results are consistent with those previously reported for other genetic resource populations, including natural accessions and recombinant inbred line. Our observations suggest that genomic changes in mutant individuals induced by gamma rays occurred at the same loci as those of natural soybean population. This study has demonstrated that the integration of GBS and GWAS can serve as a powerful complementary approach to gamma-ray mutation for the dissection of complex traits in soybean.

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Effect of Nutrient Solution Strength and Duration of Nutrient Starvation on Growth and Flowering of Two Strawberry Cultivars (양액 강도와 공급 중단 시기에 따른 삽목번식한 2품종 딸기의 생장과 개화 반응)

  • Kang, Dong Il;Jeong, Hai Kyoung;Park, Yoo Gyeong;Hwang, Seung Jae;Jeong, Byoung Ryong
    • Journal of agriculture & life science
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    • v.53 no.4
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    • pp.19-28
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    • 2019
  • This study was conducted to investigate the effect of nutrient solution strength and duration of nutrient starvation on the growth and development of strawberry (Fragaria x ananassa Duch.) 'Maehyang' and 'Sulhyang' at the flowering stage. Cuttings of runner plants were stuck on November 23th, 2017 and were covered with a layer of black plastic film to block light from penetrating and keep the relative humidity high. The black plastic film was removed after 16 days and rooted plants were cultivated for one month with irrigation of water. The Yamazaki nutrient solution with an electrical conductivity (EC) of 1.85 or 3.71 dS·m-1 (1x or 2x ionic strength, respectively) and pH 5.55 was fed to plants after either 0 (control), 1, 3 or 5 weeks of nutrient starvation to the end of experiment. Plant height in both cultivars decreased gradually with the increase in duration of nutritional starvation. The earlier the nutritional starvation started, the smaller the shoot fresh weight of 'Maehyang'. Hence the greatest shoot fresh weight was obtained in the control which was supplied with the nutrient solution continuously. Shoot fresh weight of 'Sulhyang' was the greatest in 1x ionic strength and one week of nutrient starvation before planting. Although number of flowers on the first flower cluster of 'Maehyang' and 'Sulhyang' showed no significant differences, 'Maehyang' had the greatest number of flowers in the 2x ionic strength solution and one week of nutrient starvation before planting, while 'Sulhyang' had that in the 1x ionic strength treatment. These results suggest that it is considered effective to supply a nutrient solution at a low concentration for a short period of time for increasing the number of flower differentiated on the first flower cluster in both cultivars.

Flowering Habit, Pollination Patterns and Seed Setting in Astragalus membranaceus Bunge (황기의 개화습성과 수분양식 및 결실 특성)

  • 김영국;손석용;성낙술;이봉호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.45 no.3
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    • pp.171-175
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    • 2000
  • This study was carried out to obtain the basic informations on the flowering habit, pollination patterns and seed setting in Astragalus membranaceus. The first flowering appeared at the 20-22 node on main stem and there is the indeterminate inflorescence that the flowering progress developed toward the tip of the main stem and also toward the tips of branches. According to the flowering time, the number of peduncles were increased in early of Aug. Then, the number of bearing pods and that of seed setting were increased at that time. The length of peduncles with small flower was 6.6-7.3cm, and the number of small flowers were 13-17 per peduncles. The percentage of seed setting was 30-45% per peduncles and seeds were 4.8-5.3 per pod in peduncles. On 30 days after pollination, the pod was developed with the length about 3.8cm and the width about 8mm and the seeds were 7.3per pod. According to pollination treatment, wrapping sotted no seeds and the artificial pollination sotted seeds . about 5% in late of jul. to late of Aug. but increased about 13% of seed set after early of Sep. in A. membranaceus.

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Thinning Stage on the Development of Fruit Structure in 'Fuji' Apples (적뢰 및 적과에 따른 사과 '후지' 과실의 조직 발달)

  • Park, Ji-Young;Park, Hee-Seung;Kim, Yong-Koo
    • Horticultural Science & Technology
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    • v.18 no.3
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    • pp.373-377
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    • 2000
  • This study was carried out to verify the effect of fruit thinning on fruit development, and the results were as follows. The number of cells between fruit epidermis and periphery vascular bundle during 35 to 184 days after full bloom (DAFB) were constant with 14~17 cells, regardless of thinning at pink bud stage, fruitlet stage or non-thinning. However, the distance between epidermis and vascular bundle was longest in the fruit thinned at pink stage followed by fruits thinned at fruitlet stage and control. Therefore, it was seemed that the fruit size increment during 35 to 184 DAFB was due to the increment of cell size. Thinning time affected fruit size and the earlier the thinning times were the bigger the fruits were. However, there were no direct relationships between thinning time and the starch or tannin particle development in the cells of the fruit.

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