• Title/Summary/Keyword: Day 5 ET

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Reproductive ability of minipigs as surrogates for somatic cell nuclear transfer

  • Joonho Moon;Su-Jin Kim;Jinseok Lee;Hyeyoung Kang;Bumrae Cho;Sung Joo Kim
    • Journal of Animal Science and Technology
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    • v.66 no.1
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    • pp.156-166
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    • 2024
  • Pigs are genetically, anatomically, and physiologically similar to humans. Recently, pigs are in the spotlight as a suitable source animal for xenotransplantation. However, to use pigs as source animals, pigs should be raised in designated pathogen-free facilities. There is abundant data from embryo transfer (ET) experiments using farm pigs as surrogates, but data on ET experiments using minipigs are scarce. Eighty minipigs were used for ET experiments and after transplantation, the implantation and delivery rates were investigated. It was also confirmed whether the pregnancy rate could be increased by changing the condition or surgical method of the surrogate. In the case of minipigs that gave birth, the size of the fetal sac on the 28th day of ET was also measured. The factors that can affect the pregnancy rate such as estrus synchronization program, ovulation status at the time of ET, the number of repeated ET surgeries, and the ET sites, were changed, and the differences on the pregnancy rate were observed. However there were no significant differences in pregnancy rate in minipigs. The diameter of the implanted fetal sac on the 28th day after ET in the minipigs whose delivery was confirmed was calculated to be 4.7 ± 0.5 cm. In conclusion, there were no significant differences in pregnancy rate of minipigs in the comparative experiment on various factors affecting the pregnancy rate. However, additional experiments and analyses are needed due to the large individual differences of the minipigs.

Extension Test of Midday Apparent Evapotranspiration toward Daily Value Using a Complete Remotely-Sensed Input

  • Han, Kyung-Soo;Kim, Young-Seup
    • Korean Journal of Remote Sensing
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    • v.19 no.5
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    • pp.341-349
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    • 2003
  • The so-called B-method, a simplified surface energy budget, permits calculation of daily actual evapotranspiration (ET) using remotely sensed data, such as NOAA-AVHRR. Even if the use of satellite data allows estimation of the albedo and surface temperature, this model requires meteorological data measured at ground-level to obtain the other inputs. In addition, a difficulty may be occurred by the difference of temporal scales between the net radiation in daily scale and instantaneous measurement at midday of the surface and air temperatures because the data covered whole day are necessary to obtain accumulated daily net radiation. In order to solve these problems, this study attempted a modification of B-method through an extension of hourly ET value calculated using a complete instantaneous inputs. The estimation of the daily apparent ET from newly proposed system showed a root mean square error of 0.26 mm/day as compared the output obtained from the classical model. It is evident that this may offer more rapid estimation and reduced data volume.

Effect of Lard and Corn oil on Serum total Cholesterol Content and Liver Thiobarbituric acid Value in Mice treated with Ethanol (Lard와 Corn oil이 Ethanol로 처리한 Mouse의 혈청 총 Cholesterol함량 및 간 Thiobarbituric acid치에 미치는 영향)

  • 백정희
    • Journal of the Korean Home Economics Association
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    • v.18 no.3
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    • pp.5-11
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    • 1980
  • The study carried out to clarify the effects of lard and corn oil on serum total cholesterol content and liver thiobarbituric acid (TBA) value in ethanol (Et-OH) fed mice. Results obtained from the present study were a follows: 1. serum total cholesterol content was considerably increased by 25% Et-OH administration in mice. In case of 25% Et-OH plus Lard group and Lard group both group were also increased significantly to compare with normal value of mice but 25% Et-OH plus lard group was shown very higher value rather than that of Lard group and Lard group was found similar tendency to compare with the 25% Et-OH plus saline group except to after the 1 day. 2. serum total cholestrol content of 25% Et-OH plus Corn oil group and corn oil group were also increased significantly to compare with normal value, but the 25% Et-Oh plus Corn oil group slightly higher level than that of Corn oil group except to after the 3 days. 3. Lard group was also very similar tendency to compare with the corn oil group except to the 3 days. 4. liver TBA value was increased by 25% Et-OH fed mice. 25% Et-OH plus Lard group and Lard group were also increased significantly to compare with liver TBA value in normal mice, but 25% Et-OH plus Lard group was found higher value rather than that of lard group and 25% Et-OH group (Control). Lard group was similar to that of Control group except to after the 1 day. And 25% Et-OH plus Corn oil group was considerably increased rather than that of Control group, and liver TBA value of the above group was similar to that of 25% Et-OH plus Lard group except to after the 3 days. Corn oil group was shown lower value than that of lard group, but it was no significant.

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Effects of Takju intake and moderate exercise training on brain acetylcholinesterase activity and learning ability in rats

  • Kim, Bo-Ram;Yang, Hyun-Jung;Chang, Moon-Jeong;Kim, Sun-Hee
    • Nutrition Research and Practice
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    • v.5 no.4
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    • pp.294-300
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    • 2011
  • Takju is a Korean alcoholic beverage made from rice, and is brewed with the yeast Saccharomyces cerevisiae. This study was conducted to evaluate the effects of exercise training and moderate Takju consumption on learning ability in 6-week old Sprague-Dawley male rats. The rats were treated with exercise and alcohol for 4 weeks in six separate groups as follows: non-exercised control (CC), exercised control (EC), non-exercised consuming ethanol (CA), exercised consuming ethanol (EA), non-exercised consuming Takju (CT), and exercised consuming Takju (ET). An AIN-93M diet was provided ad libitum. Exercise training was performed at a speed of 10 m/min for 15 minutes per day. Ethanol and Takju were administered daily for 6-7 hours to achieve an intake of about 10 ml after 12 hours of deprivation, and, thereafter, the animals were allowed free access to deionized water. A Y-shaped water maze was used from the third week to understand the effects of exercise and alcohol consumption on learning and memory. After sacrifice, brain acetylcholinesterase (AChE) activity was analyzed. Total caloric intake and body weight changes during the experiment were not significantly different among the groups. AChE activity was not significantly different among the groups. The number of errors for position reversal training in the maze was significantly smaller in the EA group than that in the CA and ET groups, and latency times were shorter in the EA group than those in the CC, EC, CT, and ET groups. The latency difference from the first to the fifth day was shortest in the ET group. The exercised groups showed more errors and latency than those of the non-exercised groups on the first day, but the data became equivalent from the second day. The results indicate that moderate exercise can increase memory and learning and that the combination of exercise and Takju ingestion may enhance learning ability.

Comparison of elective single cleavage-embryo transfer to elective single blastocyst-embryo transfer in human IVF-ET

  • Kang, Sang-Min;Lee, Sang-Won;Jeong, Hak-Jun;Yoon, San-Hyun;Lim, Jin-Ho;Lee, Seong-Goo
    • Clinical and Experimental Reproductive Medicine
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    • v.38 no.1
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    • pp.53-60
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    • 2011
  • Objective: This study was carried out to compare the clinical outcome of elective single cleavage-embryo transfer (eSCET) to that of elective single blastocyst-embryo transfer (eSBET) in human IVF-ET. Methods: This study was a retrospective study which analyzed for 614 women who visited the Daegu Maria Clinic from August 2008 to December 2009. All were under 37 years old and had more than 8 mm of endometrial thickness on the day of hCG administration and at least one good quality embryo on day 3. The eSCETs were performed on day 3 (n=450) and the eSBETs were conducted on day 5 (n=164). Results: The numbers of retrieved oocytes, fertilized oocytes, and day 3 good quality embryos were significantly lower in the eSCET group (12.1${\pm}$6.0, 8.2${\pm}$4.6, and 4.2${\pm}$3.1, respectively) compared to the eSBET group (16.7${\pm}$7.2, 12.1${\pm}$5.0, and 8.5${\pm}$4.5, respectively; p<0.001). However, the clinical pregnancy, implantation, on-going pregnancy, and live birth rates of the eSCET group (46.7, 46.9, 40.0, and 36.7%, respectively) were not statistically different from those of the eSBET group (51.2, 51.8, 45.1, and 43.9%, respectively; p=0.318, 0.278, 0.254, and 0.103, respectively). Conclusion: These results suggested that elective single embryo transfer should be performed regardless of the developmental stage to women less than 37 years old who had more than 8 mm of endometrial thickness on the hCG administration day and at least one good quality embryo on day 3 in order to reduce the twin pregnancy rate without reducing the whole pregnancy rate.

Evapotranspiration of Soybean-Barley Cropping as a Function of Evaporation and Available Soil Water in the Root Zone (콩 보리 작부체계하(作付體系下)에서 대기증발요구(大氣蒸發要求) 및 토양수분(土壤水分)의 함수(函數)로서의 증발산량(蒸發散量))

  • Im, Jeong-Nam;Jung, Yeong-Sang;Ryu, Kwan-Shig;Yoo, Sun-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.15 no.4
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    • pp.213-220
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    • 1982
  • Soil water changes in lysimeters with four different soils and two different available soil depths were monitored during the growing seasons of the soybean-barley cropping from 1977 to 1980 in Suweon to evaluate evapotranspiration (ET) as a function of available soil water and evaporative demand of the atmosphere. ET was calculated with soil water profile and water balance. Soil water content was measured with a neutron moisture depth gauage and The evaporative demand of the atmosphere was estimated with a class A pan evaporation. Rainfall. solar radiation, and wind speed were observed to examine heat and water balances. The average ET of soybeans ranged from 1.6 mm/day at seedling to 6.5 mm/day at flowering, and that of barley ranged from 0.5 mm/day at the regrowth stage to 4.6 mm/day at heading; however, a large variability was observed. The ratio of ET to pan evaporation ($ET/E_o$) ranged from 0.5 to 1.1 for soybeans and 0.4 to 1.2 for barley. The soil evaporation factor ($K_e$) of the $ET/E_o$ component decreased as the soil water depleted and the canopy developed. The crop transpiration factor ($K_t$), another component of $ET/E_o$, also was a function of time and the soil water. $K_t$ was constant when the available soil water fraction (f) in the root zone was greater than a threshold value, and $K_e$ was decreased linearly when f was lower than this threshold. The threshold was 0.7 for the moderate evaporative demand days, 0.4 to 0.5 for the low evaporative demand days, and 0.9 to 0.96 for the high evaporative demand days. Conclusively, the ET can be estimated from the evaporative demand of the atmosphere, $E_o$, $K_e$ and $K_t$, and the available soil water content in the root zone.

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Probable Evapotranspiration of Paddy Rice using Dry Day Index

  • 장하우;김성준
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.37 no.E
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    • pp.72-78
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    • 1995
  • To support some knowledge in planning irrigation system, short or long-term irrigation scheduling or determining irrigation reservoir capacity, it is necessary to estimate peak irrigation requirements and seasonal distribution of water demands for various return periods. In this paper Dry Day Index and Probable Evapotranspiration were evaluated to decide seasonal consumptive use of paddy rice for a design year using several decades' daily rainfall data and 5 years'('82~'86) actual evapotranspiration data, respectively. To obtain Dry Day Index that is defined as the number of probable dry days for a given period, Slade unsymmetrical distribution function was adopted. Dry Day Index was analysed for 5 and 10-day intervals. Each of them was evaluated with return periods of 1, 3, 5, 10 and 20 year. Their singnificance was tested by X$^2$ method. Based on these values, the Probable Evaportanspiration, that is the average daily ET both in dry days and rainy days during a given period, was estimated. Crop coefficient was also determined by the modified Penman equation proposed by Doorenbos & Pruitt.

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Efficacy of oxytocin antagonist infusion in improving in vitro fertilization outcomes on the day of embryo transfer: A meta-analysis

  • Kim, Seul Ki;Han, E-Jung;Kim, Sun Mie;Lee, Jung Ryeol;Jee, Byung Chul;Suh, Chang Suk;Kim, Seok Hyun
    • Clinical and Experimental Reproductive Medicine
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    • v.43 no.4
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    • pp.233-239
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    • 2016
  • Objective: Uterine contraction induced by the embryo transfer (ET) process has an adverse effect on embryo implantation. The aim of this study was to determine the effect of oxytocin antagonist supplementation on the day of ET on in vitro fertilization outcomes via a meta-analysis. Methods: We performed a meta-analysis of randomized controlled trials (RCTs). Four online databases (Embase, Medline, PubMed, and Cochrane Library) were searched through May 2015 for RCTs that investigated oxytocin antagonist supplementation on the day of ET. Studies were selected according to predefined inclusion criteria and meta-analyzed using RevMan 5.3. Only RCTs were included in this study. The main outcome measures were the clinical pregnancy rate, the implantation rate, and the miscarriage rate. Results: A total of 123 studies were reviewed and assessed for eligibility. Three RCTs, which included 1,020 patients, met the selection criteria. The implantation rate was significantly better in patients who underwent oxytocin antagonist infusion (19.8%) than in the control group (11.3%) (n = 681; odds ratio [OR], 1.92; 95% confidence interval [CI], 1.25-2.96). No significant difference was found between the two groups in the clinical pregnancy rate (n = 1,020; OR, 1.57; 95% CI, 0.92-2.67) or the miscarriage rate (n = 456; OR, 0.76; 95% CI, 0.44-1.33). Conclusion: The results of this meta-analysis of the currently available literature suggest that the administration of an oxytocin antagonist on the day of ET improves the implantation rate but not the clinical pregnancy rate or miscarriage rate. Additional, large-scale, prospective, randomized studies are necessary to confirm these findings.

Experiment for Various Soils on Economic Duty of Water in Paddy Fields (각종토성별 경제적용수량 결정시험연구)

  • Hwang, Eun
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.11 no.1
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    • pp.1561-1579
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    • 1969
  • In Korea, the duty of water in paddy fields was measured at the Agricultural Experimental Station in Suwon about 60 years ago. After that time some testing has been made in several places, but the key points in its experiment were the water depth of evapo-transpiration. Improved breeds, progress in cultivation and management techniques as well as development of measuring apparatus in recent years have necessitated the review of the duty of water in paddy fields. The necessity of reviewing the conventional methods has become even more important, as no source of information has been made available through survey of water utilization on a soil use basis which requires data on peculiar features of the water depth of evapo-transpiration. For example, the duty of water in paddy field is largely affected by the water depth of evapo-transpiration in connection with the wetted paddy field, whereas in connection with the normal paddy fields without this characteristic the vertical percolation become the predominant factor in measuring the decreasing depth of water. Therefore, it becomes important. that not only the water depth of evapotranspiration but also the vertical percolation process should also be observed in order to arrive at a realistic conclusion. As the vertical percolation has aclose relationship to the height of the underground water, the change of the latter can be measured. As the conclusion of this experiment, the following subjects are indicated. 1. In order to determine the economic duty of water in paddy fields on a basis of varying soil features, the varying soil features in the benifited area should be investigated thoroughly. The water depths of evapo-transpiration(ET) ratio to evaporation in the evaporator(V) on a basis of the varying soil features are as follows: clay loam ET/V = 1.11, loam ET/V = 1.64, sandy loam ET.V = 1.63 2. The decreasing depth of water consists of the water depth of evapotranspiration, the vertical per colation and the percolation of foot path. Among these three, the percolation of foot path can be utilized again. 3. As the result of this experiment, it shows the decreasing depth of water as follows. clay loam 9.3 mm/day, loam 13.5mm/daty, sandy loam 15.3mm/day 4. On a basis of the varying soil features and the height of the underground water, the vertical percolation varies. 5. The change of the vertical percolation on a basis of the varying soil features shows as follows: clay loam $1{\sim}2$ mm/day, loam $2{\sim}3$mm/day, sandy loam $3{\sim}4$mm/day 6. The level of the underground water changes sensibly by priority of clay loam, loam, sandy loam. When it rains, the level of the underground water rises fast and falls down slowly. 7. The level of the underground water changes within the scope of 25cm 8. The transpiration ratio is given in table 8 and their value are as follows: clay loam 168.8, loam 255.6, sandy loam 272.5

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Five Species of Fish New to Korean Waters (한국산 미기록어류 5종)

  • KIM Kyun-Huyn;KIM Yong Uk;KIM Young-Sub
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.21 no.2
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    • pp.105-112
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    • 1988
  • We found five unrecored fish species from samples collected by trawlings around the southern coastal waters of Korea from March to August, 1986 and identified them as Neoscopelus microchir Matsubara, Ariomma indica Day, Cubiceps squamiceps Lloyd, Melakichthys elegans Matsubara et Yamaguti and Bembrops caudimacula Steindachner.

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