• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 추계국제학술대회
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• pp.173-173
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• 2003

With the introduction of DNA microarrays, a high throughput analysis of gene expression is now possible as a replacement to the traditional time-consuming Southern-blot analysis. This cDNA microarray should be ahighly favored technology in the area of molecular toxicology or analysis of environmental stresses.In this study, therefore, we developed a novel cDNA microarray for analyzing stress-specific responses in japanese Medaka fish. In the design and fabrication of this stress specific functional cDNA microarray, 123 different genes in Medaka fish were selected from eighteen different stress responsive groups and spotted on a 25${\times}$75 mm glass surface. After exposure of the fish to bisphenol A which is the one of the well-known endocrine disrupting chemicals (EDCs), over 1 or 10 days, the responses of the DNA chip were found to show distinct expression patterns according to the mode of toxic actions from environmental toxicants. As a results, they showed specific gene expression pattern to bisphenol A, additionally, the chemical spesific biomarkers could be suggested based on the chip analysis data. Therefore, this chip can be used to monitor stress responses of unknown and/or known toxic chemicals using Medaka fish and may be used for the further development of biomarkers by utilizing the gene expression patterns for known contaminants.

• Journal of Astronomy and Space Sciences
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• 제13권2호
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• pp.52-66
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• 1996

본 논문에서는 우리별 2호(KITSAT-2)와 같은 소형위성의 실시간 제어 컴퓨터 시스댐인 KASCOM(KAIST satellite computer)의 설계시 요구사항(requirement)파 설계 방식(design meth-odology)을 제시한다. 위성 컴퓨터는 위성의 서브시스템으로서 뿐만 아니라 위성의 관리 제어 및 실험 장치들의 운용에 이르기까지 위성의 전 시스템파 연관을 맺고 었다. 이러한 연관성 때문에 위성 컴퓨터의 신뢰성은 위성의 전체 생명 유지에 매우 중요하다. 위성의 제어 컴퓨터로서 가져야 할 요구조건은 위성의 실시간 제어를 위한 연산 능력, 결함 허용의 입출력 시스템, 저전력소모, 무게, 크기, 방사선 차폐 등이다. 이러한 요구조건을 만족시키기 위해 KASCOM에 채택된 여러 설계 방법이 소개된다. 설계뿐만 아니라 실수 없이 구현하고 성능 및 환경 시험을 하는 것도 매우 중요하다. KASCOM의 구현 및 테스트 역시 본 논문에서 다툰다. 마지막으로 구현된 시스템의 실제 운용(in-orbit) 결파를 제시한다. 운용 결과, 프로그램 메모리(lMbit SRAM)에서는 하루 평균 2개의 SEU(lMbyte 당), 데이터 메모리(4Mbit SRAM)에서는 하루 평균 3.7개의 SEU(l Mbyte 당)가 관측되었다. 따라서 저집적 메모리가 고집적 메모리보다 SEU에 강한 것으로 보여진다.

• 한국가금학회지
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• 제44권3호
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• pp.199-209
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• 2017

Mitogen-activated protein kinase (MAPK) signaling pathways play a key role in innate immunity, inflammation, cell proliferation, cell differentiation, and cell death. The main objective of this study was to investigate the expression level of candidate MAPK pathway genes in the intestinal mucosal layer of two genetically disparate chicken lines (Marek's disease-resistant line 6.3 and Marek's disease-susceptible line 7.2) induced with necrotic enteritis (NE). Using high-throughput RNA sequencing, we investigated 178 MAPK signaling pathway related genes that were significantly and differentially expressed between the intestinal mucosal layers of the NE-afflicted and control chickens. In total, 15 MAPK pathway genes were further measured by quantitative real-time PCR(qRT-PCR) and the results were consistent with the RNA-sequencing data. All 178 identified genes were annotated through Gene Ontology and mapped onto the KEGG chicken MAPK signaling pathway. Several key genes of the MAPK pathway, ERK1/2, JNK1-3, p38 MAPK, MAP2K1-4, $NF-{\kappa}B1/2$, c-Fos, AP-1, Jun-D, and Jun, were differentially expressed in the two chicken lines. Therefore, we believe that RNA sequencing and qRT-PCR analysis provide resourceful information for future studies on MAPK signaling of genetically disparate chicken lines in response to pathogens.

• 한국가금학회지
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• 제44권3호
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• pp.211-223
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• 2017

Transforming growth factor beta ($TGF-{\beta}$) signaling pathways are involved in the regulation of proliferation, differentiation, immunity, survival, and apoptosis of many cells. The aim of this study was to investigate the differential expression of $TGF-{\beta}$-related genes, and their interactions and regulators in the spleen of two genetically disparate chicken lines (Marek's disease resistant line 6.3 and Marek's disease-susceptible line 7.2) induced with necrotic enteritis (NE) by Eimeria maxima and Clostridium perfringens infection. By using high-throughput RNA-sequencing, we investigated 76 $TGF-{\beta}$-related genes that were significantly and differentially expressed in the spleens of the chickens. Approximately 20 $TGF-{\beta}$ pathway genes were further verified by qRT-PCR, and the results were consistent with our RNA sequencing data. All 76 identified genes were analyzed through Gene Ontology and mapped onto the KEGG chicken $TGF-{\beta}$ pathway. Our results demonstrated that several key genes, including $TGF-{\beta}$1-3, bone morphogenetic proteins (BMP)1-7, inhibitor of differentiation (ID) proteins ID1-3, SMAD1-9, and Jun, showed a markedly differential expression between the two chicken lines, relative to their respective controls. We then further predicted 24 known miRNAs that targeted BMP7 mRNA from 139 known miRNAs in the two chicken lines. Among these, six miRNAs were measured by qRT-PCR. In conclusion, this study is the first to analyze most of the genes, interactions, and regulators of the $TGF-{\beta}$ pathway in the innate immune responses of NE afflicted chickens.

• 환경생물
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• 제30권2호
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• pp.77-89
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• 2012

With the advent of the genomics era powered by DNA sequencing technologies, life science is being transformed significantly and biological research and development have been accelerated. Environmental biology concerns the relationships among living organisms and their natural environment, which constitute the global biogeochemical cycle. As sustainability of the ecosystems depends on biodiversity, examining the structure and dynamics of the biotic constituents and fully grasping their genetic and metabolic capabilities are pivotal. The high-speed high-throughput next-generation sequencing can be applied to barcoding organisms either thriving or endangered and to decoding the whole genome information. Furthermore, diversity and the full gene complement of a microbial community can be elucidated and monitored through metagenomic approaches. With regard to human welfare, microbiomes of various human habitats such as gut, skin, mouth, stomach, and vagina, have been and are being scrutinized. To keep pace with the rapid increase of the sequencing capacity, various bioinformatic algorithms and software tools that even utilize supercomputers and cloud computing are being developed for processing and storage of massive data sets. Environmental genomics will be the major force in understanding the structure and function of ecosystems in nature as well as preserving, remediating, and bioprospecting them.

• Journal of Plant Biotechnology
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• 제37권2호
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• pp.115-124
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• 2010

As the completion of genome sequencing, large collection of expression data and the great efforts in annotating plant genomes, the next challenge is to systematically assign functions to all predicted genes in the genome. Functional genome analysis of plants has entered the high-throughput stage. The generations and collections of mutants at the genome-wide level form technological platform of functional genomics. However, to identify the exact function of unknown genes it is necessary to understand each gene's role in the complex orchestration of all gene activities in the plant cell. Gene function analysis therefore necessitates the analysis of temporal and spatial gene expression patterns. The most conclusive information about changes in gene expression levels can be gained from analysis of the varying qualitative and quantitative changes of messenger RNAs, proteins and metabolites. New technologies have been developed to allow fast and highly parallel measurements of these constituents of the cell that make up gene activity. We have reviewed currently employed technologies to identify unknown functions of predicted genes including map-based cloning, insertional mutagenesis, reverse genetics, chemical mutagenesis, microarray analysis, FOX-hunting system, gene silencing mutagenesis, proteomics and chemical genomics. Recent improvements in technologies for functional genomics enable whole-genome functional analysis, and thus open new avenues for studies of the regulations and functions of unknown genes in plants.

• 한국산학기술학회논문지
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• 제9권2호
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• pp.388-394
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• 2008

본 논문에서는 IEEE 802.11 WLAN(Wireless LAN)의 MAC(Medium Access Control)인 DCF(Distributed Coordination Function)의 성능을 개선하는 알고리즘을 제안하고 이를 시뮬레이션으로 분석한다. IEEE 802.11 WLAN의 MAC에서는 데이터 전송을 제어하기 위한 방법으로 DCF와 PCF(Point Coordination Function)를 사용하며, DCF의 경우 CSMA/CA(Carrier Sense Multiple Access with Collision Avoidance)를 기반으로 한다. DCF는 경쟁 스테이션이 적은 상황에서는 비교적 우수한 성능을 보이나 경쟁 스테이션의 수가 많은 경우 처리율, 지연 관점에서 성능이 저하되는 문제점이 있다. 본 논문에서는 패킷 전송 후 충돌이 발생하면 윈도우 값을 최대 CW로 증가시키고 패킷의 정상적인 전송 후에는 윈도우 값을 서서히 감소함으로써 현재 WLAN의 망 상태정보를 계속 활용함으로써 패킷 충돌 확률을 낮추는 알고리즘을 제안한다. 제안하는 알고리즘의 효율성을 입증하기 위해 시뮬레이션을 수행하여 그 타당성을 제시하였다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제4권6호
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• pp.1063-1079
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• 2010

Vehicle ad hoc networks (VANET) are one of the most important technologies to provide various ITS services. While VANET requires rapid and reliable transmission, packet transmission in VANET is unstable because of high mobility. Many routing protocols have been proposed and assessed to improve the efficiency of VANET. However, topology-based routing protocols generate heavy overhead and long delay, and position-based routing protocols have frequent packet loss due to inaccurate node position. In this paper, we propose a position-based routing repair algorithm to improve the efficiency of VANET. This algorithm is proposed based on the premise that AODV (-PGB) can be used effectively in VANET, if the discovery, maintenance and repair mechanism of AODV is optimized for the features of VANET. The main focus of this algorithm is that the relay node can determine whether its alternative node exits and judge whether the routing path is disconnected. If the relay node is about to swerve from the routing path in a multi-hop network, the node recognizes the possibility of path loss based on a defined critical domain. The node then transmits a handover packet to the next hop node, alternative nodes and previous node. The next node repairs the alternative path before path loss occurs to maintain connectivity and provide seamless service. We simulated protocols using both the ideal traffic model and the realistic traffic model to assess the proposed algorithm. The result shows that the protocols that include the proposed algorithm have fewer path losses, lower overhead, shorter delay and higher data throughput compared with other protocols in VANET.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제2권2호
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• pp.63-81
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• 2008

WiMAX has been introduced as a competitive alternative for metropolitan broadband wireless access technologies. It is connection oriented and it can provide very high data rates, large service coverage, and flexible quality of services (QoS). Due to the large number of connections and flexible QoS supported by WiMAX, the uplink access in WiMAX networks is very challenging since the medium access control (MAC) protocol must efficiently manage the bandwidth and related channel allocations. In this paper, we propose and investigate a cost-effective WiMAX bandwidth management scheme, named the WiMAX partial sharing scheme (WPSS), in order to provide good QoS while achieving better bandwidth utilization and network throughput. The proposed bandwidth management scheme is compared with a simple but inefficient scheme, named the WiMAX complete sharing scheme (WCPS). A maximum entropy (ME) based analytical model (MEAM) is proposed for the performance evaluation of the two bandwidth management schemes. The reason for using MEAM for the performance evaluation is that MEAM can efficiently model a large-scale system in which the number of stations or connections is generally very high, while the traditional simulation and analytical (e.g., Markov models) approaches cannot perform well due to the high computation complexity. We model the bandwidth management scheme as a queuing network model (QNM) that consists of interacting multiclass queues for different service classes. Closed form expressions for the state and blocking probability distributions are derived for those schemes. Simulation results verify the MEAM numerical results and show that WPSS can significantly improve the network’s performance compared to WCPS.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권11호
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• pp.5400-5418
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• 2016

Femtocells are envisioned as a key solution to embrace the ever-increasing high data rate and thus are extensively deployed. However, the dense and random deployments of femtocell access points (FAPs) induce severe intercell inference that in turn may degrade the performance of spectral efficiency. Hence, unrestrained proliferation of FAPs may not acquire a net throughput gain. Besides, given that numerous FAPs deployed in ultra-dense networks (UDNs) lead to significant energy consumption, the amount of FAPs deployed is worthy of more considerations. Nevertheless, little existing works present an analytical result regarding the optimal FAP density for a given User Equipment (UE) density. This paper explores the realistic scenario of randomly distributed FAPs in UDN and derives the coverage probability via Stochastic Geometry. From the analytical results, coverage probability is strictly increasing as the FAP-to-UE ratio increases, yet the growing rate of coverage probability decreases as the ratio grows. Therefore, we can consider a specific FAP-to-UE ratio as the point where further increasing the ratio is not cost-effective with regards to the requirements of communication systems. To reach the optimal FAP density, we can deploy FAPs in line with peak traffic and randomly switch off FAPs to keep the optimal ratio during off-peak hours. Furthermore, considering the unbalanced nature of traffic demands in the temporal and spatial domain, dynamically and carefully choosing the locations of active FAPs would provide advantages over randomization. Besides, with a huge FAP density in UDN, we have more potential choices for the locations of active FAPs and this adds to the demand for a strategic sleeping policy.