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Novel non-invasive molecular identification method for two tree frogs, Dryophytes suweonensis and Dryophytes japonicus, based on high resolution melting(HRM) analysis

  • Nakyung Yoo;Keun-Yong Kim;Jung Soo Heo;Ju-Duk Yoon;Keun-Sik Kim
    • Korean Journal of Environmental Biology
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    • v.40 no.2
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    • pp.199-205
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    • 2022
  • Two tree frogs, Dryophytes suweonensis and Dryophytes japonicus, inhabiting Korea, are morphologically similar and share the same habitats. Therefore, they are identified mainly through their calls, especially for males. Dryophytes suweonensis is registered as an endangered (IUCN: EN grade) and protected species in South Korea. Thus, it is necessary to develop a method to rapidly identify and discriminate the two species and establish efficient protection and restoration plans. We identified significant genetic variation between them by sequencing a maternally-inherited mitochondrial 12S ribosomal DNA region. Based on the sequence data, we designed a pair of primers containing 7bp differences for high resolution melting(HRM) analysis to rapidly and accurately characterize their genotypes. The HRM analysis using genomic DNA showed that the melting peak for D. suweonensis was 76.4±0.06℃, whereas that of D. japonicus was 75.0±0.05℃. The differential melt curve plot further showed a distinct difference between them. We also carried out a pilot test for the application of HRM analysis based on immersing D. suweonensis in distilled water for 30 min to generate artificial environmental DNA(eDNA). The results showed 1.10-1.31℃ differences in the melting peaks between the two tree frog samples. Therefore, this HRM analysis is rapid and accurate in identifying two tree frogs not only using their genomic DNA but also using highly non-invasive eDNA.

Associations between Attitudes toward Factors Underpinning a Successful Marriage and Marital Satisfaction among Married Men and Women in Midlife (중년 기혼남녀의 성공적 결혼 조건에 대한 태도와 결혼만족도 간 관련성)

  • Gyesook Yoo;Susanna Joo;Hayoung Park
    • Human Ecology Research
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    • v.61 no.2
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    • pp.219-231
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    • 2023
  • This study examined attitudes toward factors underpinning a successful marriage and their associations with marital satisfaction among married men and women in midlife. The sample comprised 201 married men and women aged between 40 and 59 living in Seoul, South Korea, and the data were collected by a self-report survey in 2019. Attitudes toward factors underpinning a successful marriage were measured using 12 items from the European Values Study Master Questionnaire 2008 and marital satisfaction was measured using three items from the Kansas Marital Satisfaction Scale. Exploratory and confirmatory factor analysis were performed in order to identify the factors deemed important for a successful marriage, as well as a t-test to explore gendered domains. Additionally, regression analysis was conducted to examine the association between attitudes toward factors underpinning a successful marriage and marital satisfaction. The results revealed two factors that were deemed important for a successful marriage: 'marital exchange based on conditions' and 'qualitative interaction based on sharing.' Women were more likely to believe that both factors were important for a successful marriage than men. The regression analysis revealed that 'qualitative interaction based on sharing' had a positive association with marital satisfaction, while 'marital exchange based on conditions' had a negative association with marital satisfaction, and also that these associations did not differ by gender. The findings imply that a successful marriage can be achieved when married partners make efforts to maintain their relationships through positive interactions and share intimacy in midlife.

Oral Health Behaviors and Subjective Oral Health Perception, Microorganism, and Relation between Oral Health Status

  • Hee-Sun Woo;Hye-Jung Choi
    • Journal of the Korea Society of Computer and Information
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    • v.28 no.5
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    • pp.103-111
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    • 2023
  • This study aims to analyze the relationship with oral health status through oral examinations, microorganism tests, and surveys of college students and present basic data necessary for the development of oral health education programs to prevent. Results of students who did not receive brushing education showed high motile activities of microorganism, and there was a significant relationship in which students with a large amount of microorganism subjectively felt more tooth pain. Symptoms of halitosis were greater in the DT, there were differences in the type of bacteria and the amount of bacteria in the MT, and oral health was worse if oral aids were not used in the DMFT index. Therefore, it was confirmed that oral health behavior, subjective oral health awareness, and the amount of microorganism had a significant relationship with oral health status. It will be necessary to develop and share and spread customized oral health education media for each life cycle.

Development and Operation of Remote Lone-Senior Monitoring System Based on Heterogeneous IoT Sensors and Deep Learning (이종 사물인터넷 센서와 딥러닝에 기반한 독거노인 원격 모니터링 시스템의 개발 및 운영 사례 연구)

  • Yoon, Young;Kim, Hyunmin;Lee, Siwoo;Pouri, Safa Siavash
    • Journal of the Korea Convergence Society
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    • v.13 no.1
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    • pp.387-398
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    • 2022
  • This paper presents a system that remotely monitors lone seniors at home and promptly alarms caregivers to recommend appropriate medical care services upon detecting abnormal behavior and critical conditions such as collapsing, excessive coughing, degradation of sleep quality, fever, and unusual indoor moving lines. Our system offers contactless monitoring techniques based on heterogeneous IoT sensors and deep learning to minimize the disruption to lone senior's daily life. In addition to the design and implementation of the sensor data collection and analysis system, we share our experience in installation, deployment, configuration, maintenance of the system through the case study conducted on the actual lone seniors living in Seoul Metropolitan. Based on our research, we recommend further development directions to prepare for the nationwide expansion of our system.

Climate Change and Gender Inequality in Taiwan's Green Industry: Why is Female Competency Development Mattered?

  • KIM, Hae Na;HSU, Yun-Hsiang
    • East Asian Journal of Business Economics (EAJBE)
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    • v.10 no.1
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    • pp.35-45
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    • 2022
  • Purpose - In spite of the growing importance of Taiwanese green industry, most of employees who work in this industry in Taiwan are dominated by males. Only a few females are working in this industry and their wage is lower. This research is applying role congruity theory which explains why females share only a small portion in Taiwan's green industry. This study addresses a research question, "How has the gender inequality and discrimination been reflected in the occupation structure of Taiwan's green industry? How has this gender occupation structure influenced females in the green industry?" Research design, data, and methodology - To find out the impact of gender role in the green industry, the dataset of the 2015 Taiwan Social Change Survey is used. Using STATA, t test has been implemented to address our research question with three hypotheses. Result -All of hypothesis were all supported. It is found there is a statistical difference in stereotypical thinking between female who work in the green industry and the non-green industry of Taiwan. The limited female representation in the green industry of Taiwan influences job matching and job satisfaction significantly. Conclusion - This study suggests the Taiwanese government should encourage STEM education for females and provide more relevant vocational education and training particularly for females' competency development in the green industry. By providing vocational education and training to meet the skill needs of greener economy resilient to climate change, Taiwan's green industry will grow further and will overcome gender inequality and discrimination.

A Study on Utilization Method of Information Visualization in the Humanities and Area Studies (인문·지역연구에서의 정보시각화 활용 방안 연구)

  • Kang, Ji-Hoon;Lee, Dong-Yul;Moon, Sang-Ho
    • Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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    • v.5 no.5
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    • pp.59-68
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    • 2015
  • Since interdisciplinary convergence could beyond the borders of each disciplines, it is able to create new and meaningful knowledge through collaborative research between different study areas. Especially, in recent years, the Digital Humanities has attracted the attention as the convergence form of the Humanities and ICT. From a research methodology perspective, the Digital Humanities is a tool that can be used as a convergence system for various information utilization such as storage, retrieve, share, and spread. In view of Information system, Digital Humanities has been constructed and used in a variety of systems. Among them, studies related to information visualization for the Digital Humanities have been actively conducted. To visualize data or information, various types such as images, multimedia, interface, and etc could be applied. In this paper, we analyze the cases of various information visualization in digital humanities systems, and propose a method to utilize them in the Humanities and Area Studies.

Accounting for the Water Footprint Impact of Food Waste within Korean Households

  • Adelodun, Bashir;Kim, Sang Hyun;Choi, Kyung Sook
    • Proceedings of the Korea Water Resources Association Conference
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    • 2020.06a
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    • pp.119-119
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    • 2020
  • Globally, the demand for food and water resources are increasing rapidly with the growing concerns of meeting the projected population upsurge, specifically by 2050. The global population is projected to hit 9.8 billion in 2050 while the food demand is expected to increase by 77% from the 2007 base year. Moreover, the already scarce water resources, especially in the food-producing regions, expected to be significantly affected as food production already accounts for over 70% of the global water resources. However, the estimated food demand encapsulated the actual demand for both human consumption and animal feed in addition to the exuberant food waste at the consumption stage of the supply chain, notably in the developed countries. Managing the food consumption demand and food waste can have across-the-board benefits on water resources and other associated food production impacts. This study assessed the water-saving potentials through food waste in Korean households using the food waste data obtained from the direct weighing analysis. The household food waste collection and characterization were carried out during the summer (July), fall (September), and winter (December) seasons of 2019. The water footprint related to the food waste within Korean households was based on the water footprint concept, i.e. indirect water use. The results of our estimation showed that an average Korean household wasted 6.15 ± 4.36 kg daily, amounting to 12.53 ± 11.10 m3 of water resources associated with the waste. On the per capita basis, an average of 0.024 ± 0.017 kg/capita/day of food was wasted resulting to 0.049 ± 0.044 m3/capital/day of water resources wasted. The food waste types that accounted for the principal share in the water footprint were beef, soybean, rice and pork with values 30.7, 10.1, 9.6, and 7.5%, respectively. Considering that the production of meat and meat products are water intensive and the agricultural water use in Korea is largely for rice production, addressing the food waste of these two important agricultural products can be a hotspot for water saving potential in the country. This study therefore provides an insight to addressing the water scarcity in the country through reducing household food waste.

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The Influences of Restaurant Consumers' Electronic Word-of-Mouth(E-WOM) Information Communication on Product Perception Risk, Benefit and WOM Effect

  • Heo, Yeong-Uk
    • The Journal of Economics, Marketing and Management
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    • v.6 no.4
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    • pp.51-64
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    • 2018
  • Purpose - This study is intended to look into the influences of restaurant consumers' e-WOM information communication on product perception risk, benefit and WOM effect. Research design, data, and Methodology - To achieve this, a survey was empirically carried out to 426 restaurant consumers. Results - The findings are as follows. First, the influence of e-WOM on product perception risk showed that WOM information sender characteristics, WOM information recipient characteristics and online community had a statistically significant positive influence on product perception risk. Second, the influence of e-WOM on product risk benefit showed that WOM information sender characteristics, WOM information recipient characteristics and online communication had a statistically significant positive influence on product risk benefit. Third, WOM risk perception had a statistically significant positive influence on WOM acceptance. Fourth, WOM risk benefit had a statistically significant positive influence on WOM effect. Conclusions - As for the above-mentioned findings, the communication between e-WOM sender and recipient had a positive influence on the product evaluation and attitude change in the restaurant industry, and the WOM effect had an influence on the financial performance and non-financial performance. The communication attaches importance to a direct using and tasting experience due to the nature of restaurant industry when it is simultaneously performed as a positive mechanism between sender and recipient through each channel of these factors. But the e-WOM culture can lead to the WOM effect when both sender and recipient share the persuasive communicability in reality that diversifies communication methods, having a positive influence on the management performance.

Urban Flood Simulation Considering Building and Sewer Lines (건물 및 우수 배제를 고려한 시가지 범람해석)

  • Kang, Sang-Hyeok
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.3B
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    • pp.213-219
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    • 2009
  • In densely urban areas, features such as the sewer system, buildings and river banks have an effect on flow dynamics and flood propagation, and will therefore be accounted for in the model set-up. While two-dimensional (2D) flood models of urban areas are at the forefront of current research into flood inundation mechanisms, they are however constrained by inadequate parameters of topography, and insufficient and inaccurate data. In this study, an urban flood model (overland flow, 2D urban flood flow and sewer flow) was combined and applied at Samcheok city which was damaged by inundation in 2002, in order to simulate inundation depth. The influence of buildings and pumping capacity was also analyzed to estimate the inundated depth in the study area. As a result, it was found that urban inundated depth are affected by pumping capacity directly and it increased about 20-30 cm on most of the modeled area with a building share rate of 0.2-0.6 per unit grid.

Fantastic Collaboration of Financial Services and Telecommunication: a Frontier Case of Integrated Marketing Communication of 'Club SK Card'

  • Lee, Seon Min;Chun, Seungwoo;Joo, Young Hyuck;Yoo, Changjo
    • Asia Marketing Journal
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    • v.15 no.4
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    • pp.223-241
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    • 2014
  • In May 2012, the collaboration of Hana Bank, top financial service company, and SK Planet, top telecommunication service provider, introduced a new credit card that was filled with all-in-one benefits into the market. Leveraging strong infrastructure of two companies, each top in its own industries, the awareness and preference of 'Club SK Card' brand rapidly increased to about 25% in less than one year. Moreover, this new card was enthroned in the most sold credit card of year 2012, accounting for a market share of 7.2% in the credit card market and more than 80% in the mobile credit card market. To make these results possible, 'Club SK Card' marketing team developed an effective marketing communication strategy which followed the 6M model. The mission of the marketing communication strategy was simple and clear. It was to deliver the card's inherent strengths on consumer benefits that come from the support of subsidiary and affiliated companies of SK Planet. According to OK Cashbag data, the marketing communication team selected the appropriate target consumers and approached them directly, inducing actual purchase behavior. The target consumers received straightforward messages about 'Club SK Card' and were led to join in the new membership at their most frequently visited supermarket or franchise restaurant. The straightforward communication message embedded in an eye-catching commercial ad with a hook song accompanied with a dance was delivered via public media. The ad became so popular that many other television programs quoted or made parodies of the ad. Courtesy of the commercial ad, the brand name disseminated rapidly and widely among the public. In October 2012, an ingenious planning and persistent implementation of the communication strategy results 'Club SK Card' to be ranked top in brand awareness as well as advertising preference tests.

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