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Root bark extract of Cudrania tricuspidata reduces LPS-induced inflammation in macrophages of atherogenic mice

  • Lee, Mi-Ran
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.10
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    • pp.115-123
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    • 2020
  • In this paper, we propose to evaluate the potential anti-inflammatory properties of root bark extract of Cudrania (C.) tricuspidata on lipopolysaccharide (LPS)-induced inflammation in macrophages of apolipoprotein E (ApoE) knockout (ApoE-/-) mice, murine model of atherosclerosis. Atherosclerosis is a chronic vascular inflammatory disease. C. tricuspidata is a small tree of the Moraceae family and its extract has anti-inflammatory activities. However, its role in the progress of atherosclerosis is not yet clear. To determine anti-inflammatory effects of C. tricuspidata in atherogenesis, we applied LPS in peritoneal macrophages of ApoE-/- mice and measured cell viability by CCK-8 and expression of pro-inflammatory cytokines by qRT-PCR following treatment with root bark extract of C. tricuspidata. Research data was expressed as differences between the cells treated with LPS and root bark extract and the cells treated with LPS alone (control) by a two-tailed non-parametric Mann-Whitney U-test using GraphPad Instat program. No cytotoxic effect was observed when the cells were treated with the extract at concentrations ≤ 100 ㎍/mL. The expression of inflammatory cytokines, including MCP-1, IL-1β, IFN-γ, TNF-α, and IL-6 were inhibited by the extract. These results indicated that the extract has an anti-inflammatory effect and therefore a possible role in the treatment of atherosclerosis.

Recently Augmented Natural Habitat of Abeliophyllum distichum Nakai in Yeoju-si, Gyunggi-do, Korea (미선나무(Abeliophyllum distichum Nakai)의 새로운 자생지 보고 - 경기도 여주시 자생지-)

  • Lee, Ho-Young;Kim, Tae-Gwan;Oh, Chung-Hyeon
    • Korean Journal of Environment and Ecology
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    • v.28 no.1
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    • pp.62-70
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    • 2014
  • This study was carried out to analysis the vegetation status, the habitat size and the meaning of new natural habitat, and to provide basic data for conservation or management on the newly augmented natural habitat of Abeliophyllum distichum Nakai in Yeoju-si, Gyunggi-do, Korea. A. distichum is a Korean monotypic endemic species. Most natural habitats of A. distichum have been reported in South-Central area around Chungcheong-do. The extent of occurrence of A. distichum can be enlarged to Central area due to the Yeoju-si habitat. The new habitat is located on a hillock which is composed of rock field at $37^{\circ}20{\sim}21^{\prime}N$, $137^{\circ}43{\sim}21^{\prime}E$, and 99~120m a.s.l.. The habitat size is small as about $530m^2$, and a small stream runs aside. The number of individuals of A. distichum was about 1,200. But most individuals were smaller than 0.5m height, so just about 300 individuals are taller than 1.0m height. A. distichum on Yeoju-si seemed to be maintained and distributed by vegetative propagation from elongated roots of branches. Pinus rigida and Quercus aliena dominated tree layer, and Lindera obtusiloba, Q. aliena and Acer tatricum appeared frequently in subtree layer. The shrub layer was dominated by A. distichum with Stephanandra incisa, Euonymus alatus for. ciliatodentatus, Ligustrum obtusifolium, etc. The coverage of herbaceous layer was low.

Woody Plant Species Composition, Population Structure and Carbon Sequestration Potential of the A. senegal (L.) Willd Woodland Along a Distance Gradient in North-Western Tigray, Ethiopia

  • Birhane, Emiru;Gebreslassie, Hafte;Giday, Kidane;Teweldebirhan, Sarah;Hadgu, Kiros Meles
    • Journal of Forest and Environmental Science
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    • v.36 no.2
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    • pp.91-112
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    • 2020
  • In Ethiopia, dry land vegetation including the fairly intact lowland and western escarpment woodlands occupy the largest vegetation resource of the country. These forests play a central role in environmental regulation and socio-economic assets, yet they received less scientific attention than the moist forests. This study evaluated the woody plant species composition, population structure and carbon sequestration potential of the A. senegal woodland across three distance gradients from the settlements. A total of 45 sample quadrants were laid along a systematically established nine parallel transect lines to collect vegetation and soil data across distance gradients from settlement. Mature tree dry biomass with DBH>2.5 cm was estimated using allometric equations. A total of 41 woody plant species that belong to 20 families were recorded and A. senegal was the dominant species with 56.4 IVI value. Woody plant species diversity, density and richness were significantly higher in the distant plots compared to the nearest plots to settlement (p<0.05). The cumulative DBH class distribution of all individuals had showed an interrupted inverted J-shape population pattern. There were 19 species without seedlings, 15 species without saplings and 14 species without both seedlings and saplings. A significant above ground carbon (5.3 to 12.7 ton ha-1), root carbon (1.6 to 3.6 ton ha-1), soil organic carbon (35.6 to 44.5 ton ha-1), total carbon stock (42.5 to 60.7 ton ha-1) and total carbon dioxide equivalent (157.7 to 222.8 ton ha-1) was observed consistently with an increasing of distance from settlement (p<0.05). Distance from settlement had significant and positive correlation with species diversity and carbon stock at 0.64⁎⁎ and 0.78⁎⁎. Disturbance intensity may directly influence the variation of species composition, richness and density along the A. senegal woodland. The sustainability of the A. senegal woodland needs urgent protection, conservation and restoration.

Prevalence of Human Papillomavirus Types and Phylogenetic Analysis of HPV-16 L1 Variants from Southern India

  • Kabekkodu, Shama Prasada;Bhat, Samatha;Pandey, Deeksha;Varghese, Vinay Koshy;Shukla, Vaibhav;Ghosh, Supriti;Kushtagi, Pralhad;Bhat, Parvati;Gopinath, Puthiya Mundayat;Satyamoorthy, Kapaettu
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.5
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    • pp.2073-2080
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    • 2015
  • Background: The human papillomavirus (HPV) and its variants show wide geographical distribution and have been reported to cause cervical lesions. With cervical neoplasia as the leading cancer in Indian women, the aim of the present study was to evaluate the multiple infection HPV type distribution and variant genotypes in cervical samples from the coastal Karnataka region, India. Materials and Methods: A total of 212 samples were screened by nested polymerase chain reaction using PGMY9/11 and GP5+/6+ primers. HPV positive samples were sequenced to identify the types and a phylogenetic tree was constructed using the neighbor-joining method. Results: Sequence analysis identified a total of 14 HPV types distributed in 20%, 73.3% and 82.5% of non-malignant, pre-malignant [low grade squamous intraepithelial lesion (LSIL) and high grade squamous intraepithelial lesion (HSIL)] and cervical cancer samples. The distribution of high risk HPV in cancer samples was HPV 16, 76.4%, HPV18, 11.7%, HPV81, 2.9%, HPV31, 1.4%, HPV35, 1.4% and HPV 45, 1.4%. Multiple infections were observed in 11.8% of tumor samples with HPV 16 contributing to 62.5% of cases. In non-malignant samples, 20% of HPV positive samples were detected with HPV16, 82.3%, HPV33, 5.8% and HPV58, 5.8% and very low incidence of multiple infections. Comparative phylogenetic analysis of HPV variants identified 9 HPV sequences as new papillomavirus species, predominantly classified as European lineage type. Conclusions: The findings for HPV infections associated with progression of cervical cancer in coastal Karnataka region and HPV variant analysis provide baseline data for prevention and HPV vaccination programs.

A Study on Strategy for success of tourism e-marketplace (관광 e-마켓플레이스의 성공전략에 관한 연구)

  • Hong, Ji-Whan;Kim, Keun-Hyung
    • Proceedings of the Korea Contents Association Conference
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    • 2006.11a
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    • pp.333-336
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    • 2006
  • E-marketplace is a kind of B2B e-Business system that supports business transactions among companies. If e-marketplace is revitalized, we expect not only the development of related industry but also decrease of transaction cost among companies. It is necessary for the introduction and revitalization of e-marketplace in tourist industry from this point of view. Participants of tour e-marketplace are tour-related companies(travel agencies, lodging enterprises, shipping enterprises, etc.). Also tourists want to search a variety of tour products or contents. So tour e-marketplace has characteristics of B2C e-Business systems as well as B2B e-Business systems at once. The purpose of this study is to classify success factors that determine characteristics of tour e-marketplace through statistics survey from e-marketplace factors related tourism websites. First of all, we analyze success factors of B2B and B2C e-marketplace. Then we will set up influence factors of tour e-marketplace and conduct a survey about success factors of tour e-marketplace. Therefore, we could expect to find these good attributes in tour e-marketplace success through logistic regression and decision tree analysis from source data.

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A Research on the Recognition of the Effect of Constitution-Acupuncture Based on the Expert Constitution-Acupuncture Oriental Medical Doctors (팔체질침 전문 한의사의 체질침 효과에 대한 인식조사)

  • Min, Jae-Young;Park, Young-Jae;Park, Jae-Sung;Shin, Yong-Sup;Park, Young-Bae;Lee, Sang-Chul;Kim, Min-Yong
    • The Journal of the Society of Korean Medicine Diagnostics
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    • v.11 no.1
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    • pp.105-129
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    • 2007
  • Objectives: The purpose of this research is to obtain basic data about the effects of Constitution-Acupuncture (CA) based on Eight-Constitution Medicine (ECM) by the expert CA Oriental Medical Doctors. Methods: The resources were collected from the internet survey method. 35 samples were collected. The SPSS 13.0 for windows and Answer Tree 3.0 was used for statistical analysis. Results: The results from this research are as follow. 1. The motivation that apply ECM to clinic are 'Because ECM is effective for diagnosis, treatment and prevention against disease' 48.6% and 'Because CA therapy is effective' 25.7%. 2. The advantage that CA therapy compare to the other acupuncture therapy are 'Management and prevention against disease are available by ECM theory to a person who exact constitution is confirmed' 82.9%, 'CA therapy is superior in treatment of hard symptoms to treat by the other acupuncture therapy' 80%, 'Treatment effect is good' 77.1%, 'Needle manipulation of CA is simple comparatively' 57.1%, 'It is definite whether treatment effect is good or not' 54.3% and CA therapy is superior all in diseases of twelve category. 3. When the effects of CA is good, patients expressed much 'Eyesight lightened', 'Took deep sleep at night', 'Fatigue decreased', 'Body is light', 'Feelings became refreshing' as well as 'Symptoms were improved' and also physicians confirmed as those reactions whether treatment is effective. Conclusions: The research suggest that CA is effective therapy in clinic, and so further study is needed for CA therapy.

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A Design of Fractional Motion Estimation Engine with 4×4 Block Unit of Interpolator & SAD Tree for 8K UHD H.264/AVC Encoder (8K UHD(7680×4320) H.264/AVC 부호화기를 위한 4×4블럭단위 보간 필터 및 SAD트리 기반 부화소 움직임 추정 엔진 설계)

  • Lee, Kyung-Ho;Kong, Jin-Hyeung
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.6
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    • pp.145-155
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    • 2013
  • In this paper, we proposed a $4{\times}4$ block parallel architecture of interpolation for high-performance H.264/AVC Fractional Motion Estimation in 8K UHD($7680{\times}4320$) video real time processing. To improve throughput, we design $4{\times}4$ block parallel interpolation. For supplying the $10{\times}10$ reference data for interpolation, we design 2D cache buffer which consists of the $10{\times}10$ memory arrays. We minimize redundant storage of the reference pixel by applying the Search Area Stripe Reuse scheme(SASR), and implement high-speed plane interpolator with 3-stage pipeline(Horizontal Vertical 1/2 interpolation, Diagonal 1/2 interpolation, 1/4 interpolation). The proposed architecture was simulated in 0.13um standard cell library. The gate count is 436.5Kgates. The proposed H.264/AVC Fractional Motion Estimation can support 8K UHD at 30 frames per second by running at 187MHz.

The Structure of Plant Community in Kwangnung Forest(II) - Analysis on the Forest Community in Mt. Jookyup by the Classification and Ordination Techniques - (광릉(光陵) 삼림(森林)의 식물군집구조(植物群集構造)(II) - Classification 및 Ordination방법에 의한 죽엽산지역(竹葉山地域)의 식생분석(植生分析) -)

  • Lee, Kyong Jae;Choi, Song Hyun;Jo, Jae Chang
    • Journal of Korean Society of Forest Science
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    • v.81 no.3
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    • pp.214-223
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    • 1992
  • To investigate the structure of the plant community of Mt. Jookyup area in Kwangnung forest, thirty-seven plots were set up by the clumped sampling method. The classification by TWINSPAN and two kinds of multivariate ordination(RA, DCA) were applied to the study area in order to classify them into several groups based on woody plants and environmental variables. The classification have been successfully overlayed on an ordination of the same data using DCA. The plots can be classified into five groups by TWINSPAN and DCA. The successional trends of tree species by both techniques seem to be expected two ways in the canopy layer. The first is from Pinus densiflora to Carpinus laxiflora and the second is from Pinus densiflora through Quercus mongolica to Carpinus laxiflora. In the understory layer, it was expected that Rhododendron mucronulatum ${\rightarrow}$Lindera obtusiloba, Symplocos chinensis for. pilosa, Viburunum erasum, Styrax obassia${\rightarrow}$Euonymus sachalinensis, Sorbus alnifolia. As the result of the analysis for the relationship between the stand scores of DCA and environmental variables, they had a tendency to increase significantly from the P. densiflora community to Quercus spp. community that was soil pH, total nitrogen, available phosphate and exchangeable potassium, sodium, calcium and magnesium.

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Investigation Into Reflectance Characteristics of Trees Infected by Pine Wilt Disease (소나무재선충병 감염목의 분광반사 특성 구명)

  • Kim, So-Ra;Lee, Woo-Kyun;Nam, Kijun;Song, Yongho;Yu, Hangnan;Kim, Moon-Il;Lee, Jong-Yeol;Lee, Seung-Ho
    • Journal of Korean Society of Forest Science
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    • v.102 no.4
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    • pp.499-505
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    • 2013
  • Pine wilt disease has known as a serious forest disease in East Asia such as Japan, Korea and China. Fumigation and burning are considered as best way to treat infected tree at early detection. For investigate spectral reflectance characteristics of infected trees, periodic measurement has been done in both infected and non-infected trees. Infected and non-infected trees' reflectance (400 nm~2,500 nm wavelength) are detected from June to October with GER3700 spectrometer. Noise of reflectance data was corrected using cubic spline interpolation method. Reflectance was changed in most of infected trees with ranges Red (600 nm~700 nm) and Middle Infrared (1,400 nm~1,500 nm) within two months after injected by Pine Wood nematode (PWN), but there was no differences in non-infected trees. Infected and non-infected trees were compared statistically in each period. As a result, we found that a statistically significant difference was occurred at Red and Middle Infrared (MIR) 2 months after injection (p<0.05), however, no significant difference in near infrared (p>0.05). Therefore, the early detection of infested pine trees by PWN may possible through detecting the change of spectral reflectance at red and MIR.

Removal of Methylene Blue in Water Phase by Using Juniperus chinensis (향나무를 활용한 수중에서 메틸렌 블루의 제거)

  • Choi, Suk Soon;Ha, Jeong Hyub
    • Applied Chemistry for Engineering
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    • v.29 no.3
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    • pp.278-282
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    • 2018
  • The development of treatment technologies for recycling waste woods generated from tree pruning is required in Gangwon province forest. In this study, according to adsorption experiments using three types (Larix kaemoferi, Juniperus chinensis, Pinus densiflora) of waste woods, Juniperus chinensis as a biosorbent showing an excellent removal ability was selected for the removal of methylene blue in an aqueous phase. When 0.4 g/100 mL of Juniperus chinensis was used to improve the removal efficiency of methylene blue for 4 h, each 100, 200 and 300 mg/L of methylene blue dissolved in the aqueous phase were removed to 98, 93, and 81%, respectively. The adsorption equilibrium data obtained by changing adsorbent concentrations was found to be more consistent with the Langmuir than the Freundlich equation. In addition, based on dynamic experiments by changing the methylene blue concentration, the biosorption kinetics equation was more suitable for a pseudo-second order model. In order to enhance the removal capability of highly concentrated methylene blue, 300 and 400 mg/L of methylene blue were operated for 4 h under 210 rpm of agitation velocity and removal efficiencies were 92 and 76%, respectively. Consequently, these experimental results can be effectively utilized as a new biosorption technology for economically treating methylene blue dissolved in an aqueous phase.