• The KIPS Transactions:PartA
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• 제10A권6호
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• pp.701-708
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• 2003

This paper addresses the issue involved in dependability of distributed mobile agents in the Internet environment. We propose an architectural framework for the Internet applications making mobile agents into fault-tolerant. The replication of agents and data is of great importance to achieve fault tolerance in distributed systems over the Internet. This research focuses on the replication component for the proposed fault-tolerant mobile agent framework. We present and analyze the performance results obtained when doing simulation study on the effects of the degree of replication, the active and passive replication strategies, and the replication scale.

• Journal of the Institute of Electronics and Information Engineers
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• 제51권2호
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• pp.85-91
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• 2014

Cloud storage system uses a distributed file system for storing and managing data. Traditional distributed file system makes a triplication of data in order to restore data loss in disk failure. However, enforcing data replication method increases storage utilization and causes extra I/O operations during replication process. In this paper, we propose a data replication method using erasure codes in cloud storage system to improve storage space efficiency and I/O performance. In particular, according to data access frequency, the proposed method can reduce the number of data replications but using erasure codes can keep the same data recovery performance. Experimental results show that proposed method improves performance in storage efficiency 40%, read throughput 11%, write throughput 10% better than HDFS does.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권10호
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• pp.4681-4702
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• 2016

Recently, Massive energy consumption in Cloud Storage System has attracted great attention both in industry and research community. However, most of the solutions utilize single method to reduce the energy consumption only in one aspect. This paper proposed an energy effective gear-shifting mechanism (E2GSM) in Cloud Storage System to save energy consumption from multi-aspects. E2GSM is established on data classification mechanism and data replication management strategy. Data is classified according to its properties and then be placed into the corresponding zones through the data classification mechanism. Data replication management strategies determine the minimum replica number through a mathematical model and make decision on replica placement. Based on the above data classification mechanism and replica management strategies, the energy effective gear-shifting mechanism (E2GSM) can automatically gear-shifting among the nodes. Mathematical analytical model certificates our proposed E2GSM is energy effective. Simulation experiments based on Gridsim show that the proposed gear-shifting mechanism is cost effective. Compared to the other energy-saved mechanism, our E2GSM can save energy consumption substantially at the slight expense of performance loss while meeting the QoS of user.

• Journal of Communications and Networks
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• 제16권4호
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• pp.378-384
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• 2014

Replication routing can greatly improve the data delivery performance by enabling multiple replicas of the same packet to be transmitted towards its destination simultaneously. It has been studied extensively recently and is now a widely accepted routing paradigm in delay tolerant networks (DTNs). However, in this field, the issue of how to maximize the utilization efficiency of limited replication quota in a resource-saving manner and therefore making replication routing to be more efficient in networks with limited resources has not received enough attention. In this paper, we propose a DTN routing protocol with back-pressure based replica distribution. Our protocol models the replica distribution problem from a resource allocation perspective and it utilizes the idea of back-pressure algorithm, which can be used for providing efficient network resource allocation for replication quota assignment among encountered nodes. Simulation results demonstrate that the proposed protocol significantly outperforms existing replication routing protocols in terms of packet delay and delivery ratio.

• Biomolecules & Therapeutics
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• 제30권1호
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• pp.64-71
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• 2022

Norovirus (NV) is the most common cause of viral gastroenteritis, with the potential to develop into a fatal disease in those who are immuno-compromised, and effective vaccines and treatments are still non-existent. In this study, we aimed to elucidate the molecular mechanism of the previously identified NV replication inhibitor utilizing a vinyl-stilbene backbone, AC-1858. First, we confirmed the inhibition of the NV RNA replication by a structural analog of AC-1858, AC-2288 with its exclusive cytoplasmic sub-cellular localization. We further validated the induction of one specific host factor, the phosphorylated form of heat shock factor (HSF)-1, and its increased nuclear localization by AC-1858 treatment. Finally, we verified the positive and negative impact of the siRNA-mediated downregulation and lentivirus-mediated overexpression of HSF-1 on NV RNA replication. In conclusion, these data suggest the restrictive role of the host factor HSF-1 in overall viral RNA genome replication during the NV life cycle.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 한국해양정보통신학회 2008년도 추계종합학술대회 B
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• pp.53-56
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• 2008

Recently, the distributed system is being evolved into a new paradigm, named cloud computing, which provides users with efficient computing resources and services from data centers. Cloud computing would reduce the potential danger of Grid computing which utilizes resource sharing by constructing centralized data center. In this paper, a new data replication scheme is proposed for Hadoop distributed file system by changing 1:1 data transmission to 1:N. The proposed scheme considerably reduced the data transmission delay comparing to the current mechanism.

• The KIPS Transactions:PartD
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• 제8D권5호
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• pp.605-612
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• 2001

In order to solve the overload of academic affairs management system, we propose a method builds a distributed Replication server and uses this server with the present centralized system. Normal query transactions which are not required for data modification are composed of almost all DML sentences. So we construct the distributed replication servers according to the data characteristics and make them perform the query transaction without modification. In this way, we can simultaneously distribute users and data, and cut down processing time for every transaction. Also Replication server has the advantages of implemental efficiency and economical because it uses resources of present centralized system without and additional configurations. Usually, to distribute the overload of server, they can use way, Client-side overload distribution that user program get present overload status then can choose a less overloaded server, and the other way, Server-side overload distribution that make use of Application Layer Scheduling Technique and IP Layer Scheduling Technique. Our Replication server can reduce the overload of centralized system by eliminating or complementing those defects of overload distribution, referred to in the forehead.

• Molecules and Cells
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• 제44권2호
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• pp.101-115
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• 2021

The INO80 chromatin remodeling complex has roles in many essential cellular processes, including DNA replication. However, the mechanisms that regulate INO80 in these processes remain largely unknown. We previously reported that the stability of Ino80, the catalytic ATPase subunit of INO80, is regulated by the ubiquitin proteasome system and that BRCA1-associated protein-1 (BAP1), a nuclear deubiquitinase with tumor suppressor activity, stabilizes Ino80 via deubiquitination and promotes replication fork progression. However, the E3 ubiquitin ligase that targets Ino80 for proteasomal degradation was unknown. Here, we identified the C-terminus of Hsp70-interacting protein (CHIP), the E3 ubiquitin ligase that functions in cooperation with Hsp70, as an Ino80-interacting protein. CHIP polyubiquitinates Ino80 in a manner dependent on Hsp70. Contrary to our expectation that CHIP degrades Ino80, CHIP instead stabilizes Ino80 by extending its half-life. The data suggest that CHIP stabilizes Ino80 by inhibiting degradative ubiquitination. We also show that CHIP works together with BAP1 to enhance the stabilization of Ino80, leading to its chromatin binding. Interestingly, both depletion and overexpression of CHIP compromise replication fork progression with little effect on fork stalling, as similarly observed for BAP1 and Ino80, indicating that an optimal cellular level of Ino80 is important for replication fork speed but not for replication stress suppression. This work therefore idenitifes CHIP as an E3 ubiquitin ligase that stabilizes Ino80 via nondegradative ubiquitination and suggests that CHIP and BAP1 act in concert to regulate Ino80 ubiquitination to fine-tune its stability for efficient DNA replication.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.14-14
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• 2003

Potato virus X (PVX), the type member of Potexvirus genus, is a flexuous rod-shaped virus containing a single-stranded (＋) RNA. Infection by PVX produces genomic plus- and minus-strand RNAs and two major subgenomic RNAs (sgRNAs). To understand the mechanism for PVX replication, we are studying the cis- and/or trans-acting elements required for RNA replication. Previous studies have shown that the conserved sequences located upstream of two major sgRNAs, as well as elements in the 5' non-translated region (NTR) affect accumulation of genomic and sg RNAs. Complementarity between sequences at the 5' NTR and those located upstream of two major sgRNAs and the binding of host protein(s) to the 5' NTR have shown to be important for PVX RNA replication. The 5 NTR of PVX contains single-stranded AC-rich sequence and stem-loop structure. The potential role(s) of these cis-elements on virus replication, assembly, and their interaction with viral and host protein(s) during virus infection will be discussed based on the data obtained by in vitro binding, in vitro assembly, gel shift mobility assay, host gene expression profiling using various mutants at these regions.

• Biomedical Science Letters
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• 제17권3호
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• pp.185-190
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• 2011

Adenovirus type 5 (Ad5) vectors have been used for gene transfer to a wide variety of cell types in vivo and in vitro. The advantages of adenovirus vectors include the high titer of virus readily obtained in large scale preparations, their ability to transduce dividing and non dividing cells, and the high level of transgene expression. Since adenovirus vectors do not integrate in host cell DNA, there is a lack of insertional mutagenesis. However, many human tumor cells lack expression of the adenovirus 5 receptors and contain areas of hypoxia. In order to identify the pattern of replication and gene expression of oncolytic adenovirus in hypoxic condition, multiple different fiber modified Ads (Ad5F/S11, Ad5F/S35, Ad5F/K7, Ad5F/K21, and Ad5F/RGD) was compared. The replication of all fiber modified adenovirus was inhibited in hypoxic condition in HEK 293 cells, but gene expression has variety on different tumor cell lines and the level of coxackievirus and adenovirus receptor (CAR) expression. These data suggest that CAR expression pattern and hypoxic condition of tumor are considered for optimal oncolytic adenovirus application.