• Journal of Microbiology and Biotechnology
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• v.25 no.2
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• pp.152-161
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• 2015

CodY is a highly conserved protein in low G+C gram-positive bacteria that regulates genes involved in sporulation and stationary-phase adaptation. Bacillus thuringiensis is a grampositive bacterium that forms spores and parasporal crystals during the stationary phase. To our knowledge, the regulatory mechanism of CodY in B. thuringiensis is unknown. To study the function of CodY protein in B. thuringiensis, BMB171codY^- was constructed in a BMB171 strain. A shuttle vector containing the ORF of cry1Ac10 was transformed into BMB171 and BMB171codY^-, named BMB171cry1Ac and BMB171codY^-cry1Ac, respectively. Some morphological and physiological changes of codY mutant BMB171codY^-cry1Ac were observed. A comparative proteomic analysis was conducted for both BMB171codY^-cry1Ac and BMB171cry1Ac through two-dimensional gel electrophoresis and MALDI-TOF-MS/MS analysis. The results showed that the proteins regulated by CodY are involved in microbial metabolism, including branched-chain amino acid metabolism, carbohydrate metabolism, fatty acid metabolism, and energy metabolism. Furthermore, we found CodY to be involved in sporulation, biosynthesis of poly-β-hydroxybutyrate, growth, genetic competence, and translation. According to the analysis of differentially expressed proteins, and physiological characterization of the codY mutant, we performed bacterial one-hybrid and electrophoretic mobility shift assay experiments and confirmed the direct regulation of genes by CodY, specifically those involved in metabolism of branched-chain amino acids, ribosomal recycling factor FRR, and the late competence protein ComER. Our data establish the foundation for in-depth study of the regulation of CodY in B. thuringiensis, and also offer a potential biocatalyst for functions of CodY in other bacteria.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.22 no.11
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• pp.2594-2603
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• 1997

A novel method of adaptive beam forming is presented in this paper. The proposed technique provides for a suboptimal beam pattern that increases the Signal to Noise/Interference Ratio (SNR/SIR), thus, eventually increases the capacity of the communication channel, under an assumption that the desired signal is dominant compared to each component of interferences at the receiver, which is precoditionally achieved in Code Division Multiple Access (CDMA) mobile communications by the chip correlator. The main advantages of the new technique are:(1)The procedure requires neither reference signals nor training period, (2)The signal interchoerency does not affect the performance or complexity of the entire procedure, (3)The number of antennas does not have to be greater than that of the signals of distinct arrival angles, (4)The entire procedure is iterative such that a new suboptimal beam pattern be generated upon the arrival of each new data of which the arrival angle keeps changing due tot he mobility of the signal source, (5)The total amount of computation is tremendously reduced compared to that of most conventional beam forming techniques such that the suboptimal beam pattern be produced at vevery snapshot on a real-time basis. The total computational load for generating a new set of weitht including the update of an N-by-N(N is the number of antenna elements) autocovariance matrix is $0(3N^2 + 12N)$. It can further be reduced down to O(11N) by approximating the matrix with the instantaneous signal vector.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.6A
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• pp.609-616
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• 2011

The Delay/Disruption Tolerant Network (DTN) is a network designed to operate effectively using the mobility and storage of intermediate nodes under no end-to-end guaranteed network. This new network paradigm is well-suited for networks which have unstable path and long latencies (e.g. interplanetary network, vehicular network). In this paper, we first found that each taxi has its own regularly visiting area and define this property as spatial regularity. We analyze 4000 taxi trace data in Shanghai and show the existence of spatial regularity experimentally. Based on a spatial regularity in urban environment, we present a new DTN routing method. We introduce a Weighted Center (WC) which represents spatial regularity of each node. Through the association with evenly distributed access points (APs) in urban environment, most of vehicles get their grid locations and calculate their WCs. Since our routing method only uses neighbors' WCs for building routing paths, it can be regarded as distributed and practical protocols. Our experiments involving realistic network scenarios created by the traces of about 1500 Shanghai taxies show that our routing method achieves the higher performance compared to ECT, LET by 10%~110%.

• IMMUNE NETWORK
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• v.3 no.3
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• pp.201-210
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• 2003

Objective: The role of prostaglandin $E_2$ (PGE2) in the etiopathogenesis of immune and inflammatory diseases has become the subject of recent debate. To determine the role of PGE2 in rheumatoid arthritis (RA), we tested the effect of exogenous PGE2 on the production of cyclooxygenase-2 (COX-2) by rheumatoid synoviocytes. Methods: Fibroblast-like synoviocytes (FLS) were prepared from the synovial tissues of RA patients, and cultured in the presence of PGE2. The COX-2 mRNA and protein expression levels were determined by RT-PCR and Western blot analysis, respectively. The PGE2 receptor subtypes in the FLS were analyzed by RT-PCR. Electrophoretic mobility shift assay (EMSA) was used to measure the NF-${\kappa}B$ binding activity for COX-2 transcription. The in vivoeffect of PGE2 on the development of arthritis was also tested in collagen induced arthritis (CIA) animals. Results: PGE2 ($10^{-11}$ to $10^{-5}M$) dose-dependently inhibited the expression of COX-2 mRNA and the COX-2 protein stimulated with IL-$1{\beta}$, but not COX-1 mRNA. NS-398, a selective COX-2 inhibitor, displayed an additive effect on PGE2-induced COX-2 downregulation. The FLS predominantly expressed the PGE2 receptor (EP) 2 and EP4, which mediated the COX-2 suppression by PGE2. Treatment with anti-IL-10 monoclonal antibodies partially reversed the PGE2-induced suppression of COX-2 mRNA, suggesting that IL-10 may be involved in modulating COX-2 by PGE2. Experiments using an inducer and an inhibitor of cyclic AMP (cAMP) suggest that cAMP is the major intracellular signal that mediates the regulatory effect of PGE2 on COX-2 expression. EMSA revealed that PGE2 inhibited the binding of NF-${\kappa}B$ in the COX-2 promoter via a cAMP dependent pathway. In addition, a subcutaneous injection of PGE2 twice daily for 2 weeks significantly reduced the incidence and severity of CIA as well as the production of IgG antibodies to type II collagen. Conclusion: Our data suggest that overproduced PGE2 in the RA joints may function as an autocrine regulator of its own synthesis by inhibiting COX-2 production and may, in part, play an anti-inflammatory role in the arthritic joints.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.43 no.10 s.352
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• pp.79-89
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• 2006

TCP is the most widely used transport protocol in Internet applications that guarantees a reliable data transfer. But, in the wireless multi-hop networks, TCP performance is degraded because it is designed for wired networks. The main reasons of TCP performance degradation are contention for wireless medium at the MAC layer, hidden terminal problem, exposed terminal problem, packet losses in the link layer, unfairness problem, reordering problem caused by path disconnection, bandwidth waste caused by exponential backoff of retransmission timer due to node's mobility and so on. Specially, in the mobile ad-hoc networks, discrepancy between a station's transmission range and interference range produces hidden terminal problem that decreases TCP performance greatly by limiting simultaneous transmission at a time. In this paper, we propose a new MAC algorithm for mobile ad-hoc networks to solve the problem that a node can not transmit and just increase CW by hidden terminal. In the IEEE 802.11 MAC DCF, a node increases CW exponentially when it fails to transmit, but the proposed algorithm, changes CW adaptively according to the reason of failure so we get a TCP performance enhancement. We show by ns-2 simulation that the proposed algorithm enhances the TCP performance by fairly distributing the transmission opportunity to the failed nodes by hidden terminal problems.

• The Journal of Advanced Prosthodontics
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• v.3 no.4
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• pp.229-235
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• 2011

PURPOSE. The aim of this retrospective study was to evaluate cumulative survival rate (CSR) of Implantium implants followed for 5 years and association between risk factors and the CSR. MATERIALS AND METHODS. A total of two hundred forty-nine Implantium Implants System (Dentium, Seoul, Korea) placed in ninety-five patients from 2004 to 2009 were investigated with several identified risk factors (sex, systemic disease, smoking, alchohol, reason of tooth loss, length, arch (maxilla or mandible), replace tooth type (incisor, canine, premolar or molar) Kennedy classification, prosthodontic type, prosthodontic design, opposite dentition, abutment type, occlusal material, occlusal unit, splint to tooth, cantilever, other surgery). Clinical examination (mobility, percussion, screw loosening, discomfort, etc.) and radiographic examination data were collected from patient records including all problems during follow-up period according to protocols described earlier. Life table analysis was undertaken to examine the CSR. Cox regression method was conducted to assess the association between potential risk factors and overall CSR. RESULTS. Five of 249 implants were failed. Four of these were lost before loading. The 5-year implant cumulative survival rate was 97.37%. Cox regression analysis demonstrated a significant predictive association between overall CSR and systemic disease, smoking, reason of tooth loss, arch, Kennedy classification and prosthodontic design (P<.05). The screw related complication was rare. Two abutment screw fractures were found. Another complications of prosthetic components were porcelain fracture, resin facing fracture and denture fracture (n=19). CONCLUSION. The 5-year CSR of Implantium implants was 97.37 %. Implant survival may be dependent upon systemic disease, smoking reason of tooth loss, arch, Kennedy classification and prosthodontic design (P<.05). The presence of systemic diseases and combination of other surgical procedures may be associated with increased implant failure.

• Journal of Periodontal and Implant Science
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• v.44 no.1
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• pp.13-19
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• 2014

Purpose: The purpose of this study was to retrospectively evaluate the survival of periodontally hopeless teeth that were intentionally extracted and replanted after a delay and to compare the radiographic characteristics of the survival group with those of the failure group. Methods: The clinical and radiographic data from patients who underwent delayed intentional replantation between March 2000 and July 2010 were reviewed. Twenty-seven periodontally hopeless teeth were extracted and preserved in medium supplemented with antibiotics for 10-14 days. The teeth were then repositioned in the partially healed extraction socket and followed for 3 to 21 months. The radiographic parameters were analyzed using a paired t test and the cumulative survival rate was analyzed using Kaplan-Meier analysis. Results: Seven replanted teeth failed and the overall cumulative survival rate was 66.4%. In the survival group, the amount of bone loss was reduced from 68.45% to 34.66% three months after replantation. There was radiologic and clinical evidence of ankylosis with 5 teeth. However, no root resorption was found throughout the follow-up period. In the failure group, bone formation occurred from the bottom of the socket. However, a remarkable radiolucent line along the root of a replanted tooth existed. The line lengthened and thickened as time passed. Finally, in each case of failure, the tooth was extracted due to signs of inflammation and increased mobility. Conclusions: Delayed intentional replantation has many advantages compared to immediate intentional replantation and could serve as an alternative treatment for periodontally involved hopeless teeth. However, techniques for maintaining the vitality of periodontal structures on the tooth surface should be developed for improved and predictable results.

• The Journal of Korean society of community based occupational therapy
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• v.1 no.1
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• pp.7-17
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• 2011

Objective : This study aimed to understand the experience of taking a bus, investigate successful strategies to overcome the psychological difficulties of people with disabilities. Method : In this study, a phenomenological and hermeneutic approach was used to gain a deeper understanding of the behavior, the language, the process. For participants through interviews and participant observation data were collected. Phenomenological interpretation of the way through technology and to evaluate the results. Result : Ambulatory persons with disabilities in the community have confidence in the bus-independent movement in the community possible. And get on and off the bus at the moment of urgent strategies to use in the bus seats were taken. To cope with unexpected situations like that do not put stress was confirmed. In addition, by bus and go sit on the seat to look at the patient's burden was to. Conclusion : Ambulant disabled people's confidence in the bus had a significant impact on the independent movement. Always on the lookout for falling, and many psychological difficulties and to overcome unforeseen circumstances to predict and know that public transportation could be.

• Journal of the Korean Society of Radiology
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• v.1 no.2
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• pp.13-22
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• 2007

The purpose of this study was to survey a development of the wireless transmission system of medical images for ubiquitous medicine. There have been many changes in medical equipments and medical record medical treatment and medical record within hospital and PACS(Picture Archiving Communication System) which is picture management system for patients can be typical cases. It is difficult to use these automated medical systems unless they are within hospital and in case of rapid image reading in the emergency cases or in absence of doctor, it is difficult to perform it immediately. The present study implemented an image transmission system using CDMA connection so that images in the server can be viewed at any time and in any place. Remote wireless diagnosis based on medical images using PDA is applicable to medical areas that require mobility, and the use of PDA can be an ideal alternative for point of care. The use of PDA enables prompt and accurate access to digital medical images, which in turn reduces medical accidents and improves the quality of medical services through high productivity and efficiency of medical practitioners' works. It also enables quick response to patients' demands and high-quality medical services and, consequently, patients' high satisfaction.

• Molecules and Cells
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• v.38 no.3
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• pp.259-266
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• 2015

The regulation of flowering time has crucial implications for plant fitness. MicroRNA156 (miR156) represses the floral transition in Arabidopsis thaliana, but the mechanisms regulating its transcription remain unclear. Here, we show that two AGAMOUS-like proteins, AGL15 and AGL18, act as positive regulators of the expression of MIR156. Small RNA northern blot analysis revealed a significant decrease in the levels of mature miR156 in agl15 agl18 double mutants, but not in the single mutants, suggesting that AGL15 and AGL18 co-regulate miR156 expression. Histochemical analysis further indicated that the double mutants showed a reduction in MIR156 promoter strength. The double mutants also showed reduced abundance of pri-miR156a and pri-miR156c, two of the primary transcripts from MIR156 genes. Electrophoretic mobility shift assays demonstrated that AGL15 directly associated with the CArG motifs in the MIR156a/c promoters. AGL18 did not show binding affinity to the CArG motifs, but pull-down and yeast two-hybrid assays showed that AGL18 forms a heterodimer with AGL15. GFP reporter assays and bimolecular fluorescence complementation (BiFC) showed that AGL15 and AGL18 co-localize in the nucleus and confirmed their in vivo interaction. Overexpression of miR156 did not affect the levels of AGL15 and AGL18 transcripts. Taking these data together, we present a model for the transcriptional regulation of MIR156. In this model, AGL15 and AGL18 may form a complex along with other proteins, and bind to the CArG motifs of the promoters of MIR156 to activate the MIR156 expression.