• Journal of Life Science
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• 제11권2호
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• pp.83-86
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• 2001

The long terminal repeat (LTR) elements of human endogenous retrovirus (HERV) have been found to be coexpressed with genes located nearby. It has been suggested that the LTR elements have contributed to the genetic variation of human genome connected to various diseases. Recently, HERV-W family was identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Using genomic DNAs derived from schizophrenia, we performed PCR amplification and identified six HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (87.7-99.5%) with HERV-W LTR (AF072500). Sequence analysis of the HERV-W LTR elements revealed that clone W-sch1 showed identical sequence with the AC003014 (PAC clone RP1-290B4) derived from human Xq23. Clone W-sch2 was closely related to the AC0072442 derived from human Y chromosome by phylogenetic analysis. Our data suggest that new HERV-W LTR elements in schizophrenia may be very useful for further studies to understand neuropsychiatric diseases.

• Mycobiology
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• 제31권4호
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• pp.196-199
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• 2003

A very reliable and specific method for the identification of fungi in ectotrophic mycorrhizal symbiosis was developed using a specific PCR assay based on the amplification of the ITS1 region. To obtain specific data, an ITS-diagnostic assay was carried out that reveals genera and species specific sequences. Here, an application of one method is presented, which covers the identification of pure mycelia, basidiocarps as well as mixed samples such as ectomycorrhizal roots that were mingled with remains of the host plant. For this purpose a protocol was established that allowed the extraction of DNA from single mycorrhizal roots. In order to perform a specific ITS analysis we generated a new ITS-primer(ITS8) by a multiple alignment of five different genera and species of mycorrhizal fungi. The utilization of ITS1 and ITS8 resulted in specific PCR amplicons, which were characterized by sequencing without purification steps, even when the template DNA was associated with roots.

• Animal cells and systems
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• 제6권2호
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• pp.167-170
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• 2002

The long terminal repeats (LTRs) of human endogenous retrovirus (HERV) have been found to be coexpressed with sequences of closely located genes. It has been suggested that the LTR elements have contributed to the structural change or genetic variation of human genome connected to various diseases and evolution. We examined the HERV-W LTR elements in various cancer cells (2F7, A43l , A549, HepG2, MIA-PaCa-2, PC-3, RT4, SiHa, U-937, and UO-31). Using genomic DNA from the cancer cells, we performed PCR amplification and identified twelve new HERV-W LTR elements. Those LTR elements showed a high degree of sequence similarity (88-99％) with HERV-W LTR (AF072500). A phylogenetic tree obtained by the neighbor-joining method revealed that HERV-W LTR elements could be mainly divided into two groups through evolutionary divergence. Three HERV-W LTR elements (RT4-2, A43l-1, and UO3l-2) belonged to Group 1, whereas nine LTR elements (2F7-2, A549-1, A549-3, HepG2-3, MP2-2, PC3-1, SiHa-8, SiHa-10, and U937-1) belonged to Group 11. Taken together, our new sequence data of the HERV-W LTR elements may contribute to an understanding of tissue-specific cancer by genomic instability of LTR integration.

• 한국해양바이오학회지
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• 제2권4호
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• pp.263-266
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• 2007

Vibrio vulnificusis a causative agent of serious diseases in humans resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the rpoS gene, a general stress regulator, to detect V. vulnificus. PCR specificity was identified by amplification of 8 V. vulnificus templates and by the loss of a PCR product with 36 non-V. vulnificus strains. The PCR assay had the 273-bp fragment and the sensitivity of 10 pg DNA from V. vulnificus. SYBR Green I-based real-time PCR assay targeting the rpoS gene showed a melting temperature of approximately $84^{\circ}C$ for V. vulnificus strains. The minimum level of detection by real-time PCR was 2 pg of purified genomic DNA, or $10^3$ V. vulnificus cells from pure cultured broth and $10^3$ cells in 1g of oyster tissue homogenates. These data indicate that real-time PCR is a sensitive, species-specific, and rapid method for detecting this bacterium using the rpoS gene in pure cultures and in infected oyster tissues.

• 한국지진공학회논문집
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• 제20권7_spc호
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• pp.537-543
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• 2016

In low to moderate seismic regions, there are limited earthquake ground motion data recorded from past earthquakes. In this regard, the Gyeongju earthquake (M=5.8)occurred on September 12, 2016 produces valuable information on ground motions. Ground motions were recorded at various recording stations located widely in Korean peninsula. Without actual recoded ground motions, it is impossible to make a ground motion prediction model. In this study, a point source model is constructed to accurately simulate ground motions recorded at different stations located on different soil conditions during the Gyeongju earthquake. Using the model, ground motions are generated at all grid locations of Korean peninsula. Each grid size has $0.1^{\circ}(latitude){\times}0.1^{\circ}(longitude)$. Then a contour hazard map is constructed using the peak ground acceleration of the simulated ground motions.

• 전기학회논문지
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• 제65권5호
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• pp.857-861
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• 2016

In this study, we proposed a new method that can be measure ECG (Electrocardiography) and PPG (Photoplethysmography) in realtime on the site of the wrist for check the state of health in daily life. For convenience measurement of ECG the lead I method was used on the wrist, and omit the reference junction ECG I was measured in the right hand and the left hand of the potential difference. Then the measured electrocardiogram was amplified by the differential amplifier and the signals were passed HPF, LPF, and BPF filters. For removing the PPG's noise from the Motion artifact and temperature, we apply the reflective photoelectric volume pulse wave measurement method using green LED as a light source. The circuits was designed to be able to check the waveform using higher active amplification method at weak signals. For the validation of our device, the measured signals were compared with E2-KIT on same time. The results shows that the error does not exceed the maximum one, most of the data is confirmed to be issued Peak inspection of the same number.

• 혜화의학회지
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• 제9권2호
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• pp.315-324
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• 2001

To investigate the capacity of anti-inflammatory cytokines and biological response modifiers (BRMs) to induce IL-$1{\beta}$, IL-6, TNF-${\alpha}$ gene overexpression from mouse macrophages, we isolated the resident peritoneal macrophages from BALB/c mouse (8 week old) and incubated for 6 h with lipopolysaccaride (LPS) and Moschus moschiferus (MOMS) extracts. Analysis of inflammatory cytokines gene expression was carried out by RT-PCR amplification. Amplified PCR products were electrophoresed on 1.2% agarose gel, and the analysis (Ht) was used to 1D-density program. 1. LPS and MOMS extract treatments resulted in a significant decrease in IL-$1{\beta}$, IL-6, TNF-${\alpha}$ mRNA expression level compared with the LPS treatment. 2. Among four sample of MOMS, Inhibitory effects of MOMS-A and MOMS-D for inflammatory cytokines gene expression were to be fine compared with the MOMS-Band MOMS-C. According to the above data, Because the anti- tumoral and anti-inflammatory response activities of macrophage are known to be dependent on the production of inflammatory cytokines (IL-$1{\beta}$, IL-6, TNF-${\alpha}$) by macrophages, we suggest that evaluations of BRM for the reduction of inflammatory cytokines production by macrophages are important for clinical application.

• 식물분류학회지
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• 제50권1호
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• pp.22-26
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• 2020

Chrysosplenium aureobracteatum Y. I. Kim & Y. D. Kim (Saxifragaceae) is a recently described endemic species growing in the central part of the Korean peninsula. It requires constant monitoring for conservation due to its limited distributions. There is also a need for molecular markers for proper assessments of the genetic differentiation of C. aureobracteatum from species morphologically similar to it. In this study, we developed microsatellite markers that can be used to evaluate the genetic diversity of this species, representing fundamental data with which to conserve the natural populations of the species. A total of 17 expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed by the Illumina pair-end sequencing of the transcriptomes of C. aureobracteatum. These markers were successfully applied to populations of C. aureobracteatum and to its most closely related species, C. barbatum, revealing high polymorphism in both species. The EST-SSR markers developed in this study were proven to be useful not only to monitor the population genetic structure of C. aureobracteatum for conservation purposes but also to study the genetic delimitation of the species from species closely related to it.

• Earthquakes and Structures
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• 제13권1호
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• pp.79-87
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• 2017

Seismic analysis of local site conditions is fundamental for a reliable site seismic hazard assessment. It plays a major role in mitigation of seismic damage potential through the prediction of surface ground motion in terms of amplitude, frequency content and duration. Such analysis requires the determination of the transfer function, which is a simple tool for characterizing a soil profile by estimating its vibration frequencies and its amplification potential. In this study, numerical simulations are carried out and are then combined with a statistical study to allow the characterization of design sites classified by the Algerian Building Seismic Code (RPA99, ver 2003), by average transfer functions. The mean transfer functions are thereafter used to compute RPA99 average site factors. In this regard, coming up seismic fields are simulated based on Power Spectral Density Functions (PSDF) defined at the rock basement. Results are also used to compute average site factor where, actual and synthetic time histories are introduced. In absence of measurement data, it is found that the proposed approach can be used for a better soil characterization.

• 한국해안해양공학회지
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• 제7권1호
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• pp.46-56
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• 1995

묵호항내 수면교란 현상을 분석하기 위하여 묵초항의 항외, 항입구 및 항내의 3개 정점에서 2회에 걸쳐 약 10일간 수압식 파고계를 이용하여 현상관측을 실시하였다. 관측자료의 스펙트럼 분석 결과 묵호항의 Helmholtz period는 약 10.0-14.3분, 제2 공진주기는 약 3.3분이 되는 것으로 나타났다. 정점 S3에서 Helmholtz period에서의 평균 증폭비는 약 6.8, 관측기간중의 항내에서의 해수위 변위 진폭은 약 5-10cm로 나타났다. 이러한 묵호항의 Helmholtz period 및 제2 첨단주기는 수치모형(정 등, 1993)으로 계산한 결과와 잘 일치하였다. 또한 수치모형실험을 통하여 묵호항의 제1 및 제3 공진주기로 7.5분과 1.9분을 각각 제시하였다.