• 제목/요약/키워드: Dark germination

검색결과 180건 처리시간 0.021초

식물공장 시스템을 이용한 약초의 채소화에 관한 기초연구 1. 채소화가능 약초종자의 발아에 미치는 온도 및 광의 영향 (A Study on Utilization of Medicinal Herb as Vegetable by Hydroponics I. The effect of light and temperature on the seed germination of medicinal herb.)

  • 최성규
    • 한국자원식물학회지
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    • 제7권2호
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    • pp.137-141
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    • 1994
  • In order to establish utilijzation of medicinal herb as vegetable by Hydroponics, the present studies were conducted to investigate the effect of temperature and light on the seed germination ofmedicinal herb. The results obtained are summaried as follows. The lower and upper temperaturelimit for herb germination was 10'c and 30'c within which the optimum was 20'C. Germinationof medicinal herb was similar in light and dark condition.

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맥아의 ${\alpha}-Amylase$ isozyme에 미치는 Red Light의 영향 (Effects of Red light on ${\alpha}-Amylase$ isozymes of the Germinated Barley (Hordeum distichum L.))

  • 김진구;신승렬;김광수;손태화
    • Applied Biological Chemistry
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    • 제31권4호
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    • pp.351-355
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    • 1988
  • 이조대맥(Hordeum distichum L. emend Lamark)에 적색광을 조사하여 발아일수에 따른 ${\alpha}-amylase$의 활성 및 isozymes에 미치는 적색광의 영향을 조사하였다. ${\alpha}-amylase$는 암소에서 비활성도가 발아 3일과 5일에 각각 355.0, 523.7 $units/mg{\cdot}protein$로 발아일수가 경과함에 따라 증가하였으며, 적색광조사에 의해서 비활성도가 발아 3일과 5일에 각각 암소에 비하여 48%, 15% 정도 증가하였다. CM-cellulose와 Sephacryl S-200에 의해 2종의 isozyme이 분리되었고 그 중 ${\alpha}-amylase$ I이 주 효소였다. ${\alpha}-amylase$ I, II의 활성비율은 암소에서 발아 3일과 5일에 다 같이 95 : 5 정도였으나 적색광조사구에서는 각각 60 : 40, 90 : 10으로 현저한 차이가 있었다. 특히 적색광조사에 의해서발아 3일에 ${\alpha}-amylase$ II의 활성비율이 현저히 높았다.

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파종전후 종자에 가해지는 광질, $GA_3$ 및 온도에 따른 담배의 발아율 (Effect of Light Quality,$GA_3$ and Temperature as Treatments Before or During Germination on Tobacco Seed Germinability)

  • 강진호
    • 한국자원식물학회지
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    • 제11권2호
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    • pp.124-130
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    • 1998
  • It failed occasionally to take a reasonable emergence rate since tobacco (Nicotiana tabacum L.) seeds were planted on late Jan. showing lowest temperature. This experiment was done to measure the effect of GA3 (concentration ; period) , light quality (red ; white ; dark) during or after its treatment, daily irradiation hours( 0 ; 8 ; 12 ;16) and germination termperature (20 or 10 $^{\circ}C$ ocnstant ; 20/1$0^{\circ}C$ alternating) on the germination rate. Red and white light given during grmination showed no differences between the other daily irradiation hours except that 8 hours red light delayed germination although their 12 hours irradiation had the gratest rate. The rate was increased with increased concentration to GA3 0.01 mM or increased imbibition period to 3 days although the rate of cv. NC 82 was less than that of cv. Burley 21 in the case of dark imbibition of GA3 but daily 12 hours irradiation during germination. Light quality forced during GA3 imbibition eliminated such effect of GA3 shown in the darkness so that only light quality pretreatment and termperature during germinition were affected on the rate. The germination rate of thecultivars was decreased in the order of red, white light, darkness meaning that it was highly influenced by the light quality during GA3 treatment. Regardless of GA3 or light quality treatment,on the other hand, the rate was greater in 20 $^{\circ}C$ constat than 1$0^{\circ}C$ constant and 20/1$0^{\circ}C$ alternating germination temperature having similar germinative patterns.

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Effect of dark incubation in germination of indirect date palm somatic embryos and conversion into plantlets

  • Mansour Abohatem;Yousra Al-Qubati;Hanan Abohatem
    • Journal of Plant Biotechnology
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    • 제50권
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    • pp.267-274
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    • 2023
  • All studies on date palm somatic embryogenesis have focused on germination in the presence of light while neglecting germination in darkness, which mimics the germination process of zygotic embryos within seeds. To improve the date palm micropropagation protocol, we investigated the effects of light and darkness incubation on the germination of indirect date palm somatic embryos and their subsequent conversion into plantlets. Darkness incubation emerged as a pivotal factor in the germination of indirect date palm somatic embryos and their successful conversion into plantlets. Darkness incubation significantly decreased the time required for the conversion of indirect somatic embryos into plantlets, halving the duration from 24 weeks to only 12 weeks. The micropropagation protocol was modified, consolidating the previous two distinct stages of germination and elongation under light incubation into a single stage under darkness incubation. These findings modified the protocol and significantly reduced the overall duration of the date palm micropropagation protocol.

암조건에서 발아시킨 은하콩의 발아특성 및 아이소플라본 함량 변화 (Changes in Isoflavones and Germination Characteristics of Eunhakong (Glycine max) by Germinated under Dark Condition)

  • 최원석;최선정;최웅규
    • 한국식품영양학회지
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    • 제26권2호
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    • pp.318-322
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    • 2013
  • 본 연구에서는 암조건에서 은하콩의 발아시 발아 특성과 색도 변화 및 아이소플라본 함량의 변화를 확인하였다. 발아수율은 발아 시간이 점차 증가함에 따라 수율도 높아져서 발아 60시간째에는 $299.7{\pm}15.0%$를 나타내었다. 건조 중량은 발아가 진행됨에 따라 지속적으로 감소하여 발아 60시간째에는 $65.8{\pm}8.2%$를 나타내었다. 뿌리길이는 발아 초기에서 보다는 발아 후기에 급격한 증가 패턴을 보이는 것으로 확인되었다. 대부분의 콩은 발아 60시간 후에는 발아가 이루어진 것을 확인할 수 있었다. L값은 발아 12시간째에 비교적 높은 폭으로 증가하였으며, 발아 60시간째에 $63.4{\pm}0.7$까지 상승하는 것으로 확인되었다. a값은 $-2.6{\pm}0.3$으로 나타났으며, 발아 초기에 a값은 급격히 증가하였다가 이후 감소하는 패턴을 보였다. 수침한 콩의 b값은 발아 12시간째에 큰 폭으로 증가하였다가 그 이후는 큰 변화가 없는 것으로 확인되었다. 은하콩의 총 아이소플라본 함량은 발아 12시간째에 급격히 증가한 후 감소하였으며, 48시간까지는 발아시키지 않은 콩에 비해서는 높은 함량을 유지하였다. 함량별로는 m-genistin > daidzin > m-daizin > genistein의 순이었다.

참취의 종자발아에 관한 연구 (The Germination of Aster scaber THUNB)

  • 박석근
    • 한국자원식물학회지
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    • 제7권1호
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    • pp.73-76
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    • 1994
  • This study was carried out to investigate the morphological craracteristics of seed and effect of Gibberellin and light in ffter rooter.The results obtained were summerized as follows :1, 000 seeds wt. was 1.9g, 1 liter wt. was 41.2g, seed length was 3.6mm, seed width was 1.1mm and seed thickness was 0.8mm.The germination rate was higher with light condition than dark and Gibberellin treatment waseffective.

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Seed Germination and Dormancy Breaking of Thalictrum rochebrunianum var. grandisepalum (H. Lev.) Nakai

  • Cho, Ju Sung;Kwon, Hyuk Joon;Lee, Cheol Hee
    • 한국자원식물학회지
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    • 제29권3호
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    • pp.339-346
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    • 2016
  • This study was carried out to develop an effective seed propagation method for Thalictrum rochebrunianum var. grandisepalum (H. Lev.) Nakai by analyzing seed dormancy types and germination characteristics. Seeds were collected between September to October at Gangwon province, and well-selected seeds were used while being dry-stored at 4±1℃. The seed size ranged 4.52 × 1.58 ㎜ and the weight of thousand seeds were 1,603.5 ± 0.02 ㎎. The moisture content was 7.2%. Seeds were achene type, and morphology characters showed an elliptical shape and rough texture, and light brown in color. Moist-chilling treatment was conducted for dormancy breaking because the seeds had an undeveloped embryo of liner type. The embryo had developed during a moist-chilling period, constantly, and fully developed in 10 weeks. Consequently, it seemed to be non-deep complex or intermediate complex type of morphophysiological dormancy, and embryo dormancy was broken by wet-chilling for 10 weeks. After 10 weeks of wet-chilling treatment, seed germination began. Germination percentage was higher in dark condition raher than light condition and recorded the maximum at 25℃ in the dark (16.3%). A pre-soaking treatment with a combined plant growth hormones promoted germination and shortened T50. Specifically, seed germination of 84.5% was achieved by pre-soaking of seeds with a combined solution of 500 ㎎/L GA3 and 10 ㎎/L kinetin for 24 h after a wet-chilling treatment for 10 weeks. Thus the effect of plant growth hormones coupled with chilling temperature on seed breaking dormancy provide asubsequent growth of seedlings for successful plantation.

ISTA 변온조건에서 퍼레니얼 라이그래스 신품종 8종류의 발아특성 및 일일 발아패턴 (Germination Characteristics and Daily Seed Germinating Pattern of 8 New Varieties of Perennial Ryegrass under Alternative Conditions Required by ISTA)

  • 김경남;정기완
    • 한국환경복원기술학회지
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    • 제12권3호
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    • pp.72-82
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    • 2009
  • Research was initiated to investigate early establislment characteristics and germination pattern of perennial ryegrass (PR, Lolium perenne L.). Eight varieties of PR were evaluated in the study. An alternative environmental condition for a PR germination test required by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination pattern were measured and analyzed daily for 30 days. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 75% germination, and final germination rate. Germination rates were variable among the varieties, being 66.00 to 93.75% at the end of study. There were considerable variations in early germination characteristics and germination pattern among PR varieties. The first germination in all entries tested was initiated from 3 to 5 DAS (days after seeding) under ISTA conditions, being 2 days in differences among the varieties. It was 4 to 7 DAS in days to the 50% germination, being 1 to 3 days later from the first germination and three days were variable among them. Days to the 75% germination were 5.93 to 10.50 DAS under ISTA conditions, being 4.57 days in variety differences. Pennant II and Brightstar II were the fastest varieties. The slowest ones were Revenge GLX and Brightstar SLT. Considering days to the first germination, days to 75% germination, and germination pattern etc, Pennant II and Brightstar II were regarded as excellent varieties under ISTA conditions. From tills study, information on differences in germination characteristics and pattern would be usefully applied for golf course design and construction, when established with PR.

들깨의 발아가 들깨지방질의 특성에 미치는 영향 (Effects of Seed Germination on Characteristics of Perilla Seed Lipids)

  • 최은옥;황현숙
    • 한국식품조리과학회지
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    • 제27권3호
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    • pp.51-57
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    • 2011
  • Color, lipid and fatty acid composition, and tocopherols and polyphenols contents of perilla seed lipids in response to seed germination were studied. Perilla seeds were germinated at $30^{\circ}C$ in the dark for 12, 36, or 48 h, after which total lipids were extracted by the Folch method using chloroform and methanol (2:1, v/v). Seed germination resulted in a decrease in yellowness and greenness in perilla seed lipids, but there were no significant changes in composition of the lipids including major neutral lipids (>90%). Contents of phosphatidylcholine and phosphatidylethanolamine in the perilla seed lipids significantly increased in response to germination. Linolenic acid (>63%) was the most abundant fatty acid. Seed germination tended to decrease the relative content of linolenic acid and increase the contents of oleic and stearic acids. Contents of antioxidants, especially ${\alpha}$-tocopherol and polyphenols, increased in response to seed germination. As the germination period was extended, the antioxidant content increased. Therefore, increases in useful components, phosphatidylcholine, phosphatidylethanolamine, ${\alpha}$-tocopherol, and polyphenols contents by seed germination can contribute to the improvement of perilla seed utilization in food industry.

Estimation of Cardinal Temperatures for Germination of Seeds from the Common Ice Plant Using Bilinear, Parabolic, and Beta Distribution Models

  • Cha, Mi-Kyung;Park, Kyoung Sub;Cho, Young-Yeol
    • 원예과학기술지
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    • 제34권2호
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    • pp.236-241
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    • 2016
  • The common ice plant (Mesembryanthemum crystallinum L.) has some medicinal uses and recommended plant in closed-type plant factory. The objective of this study was to estimate the cardinal temperatures for seed germination of the common ice plant using bilinear, parabolic, and beta distribution models. Seeds of the common ice plant were germinated in the dark in a growth chamber at four constant temperatures: 16, 20, 24, and $28^{\circ}C$. For this, four replicates of 100 seeds were placed on two layers of filter paper in a 9-cm petri dish and radicle emergence of 0.1 mm was scored as germination. The times to 50% germination were 4.3, 2.5, 2.0, and 1.8 days at 16, 20, 24, and $28^{\circ}C$, respectively, indicating that the germination of this warm-weather crop increased with temperature. Next, the time course of germination was modeled using a logistic function. For the selection of an accurate model, seeds were germinated in the dark at constant temperatures of 6, 12, 32, and $36^{\circ}C$. Germination started earlier and increased rapidly at temperatures above $20^{\circ}C$. The minimum, optimal, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate, as a function of the temperature gradient. The different functions estimated differing minimum, optimal and maximum temperatures, with 5.7, 27.7, and $36.5^{\circ}C$, respectively for the bilinear function, 13.4, 25.0, and $36.6^{\circ}C$, respectively, for the parabolic function and 7.8, 25.9, and $36.0^{\circ}C$, respectively, for the beta distribution function. The models estimated that the inverse of time to 50% germination rate was 0 at 6 and $36^{\circ}C$. The observed final germination rates at 12 and $32^{\circ}C$ were 62 and 97%, respectively. Our data show that a beta distribution function provides a useful model for estimating the cardinal temperatures for germination of seed from the common ice plant.