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Changes in Isoflavones and Germination Characteristics of Eunhakong (Glycine max) by Germinated under Dark Condition

암조건에서 발아시킨 은하콩의 발아특성 및 아이소플라본 함량 변화

  • Choi, Won-Seok (Dept. of Food Science & Technology, Korea National University of Transportation) ;
  • Choi, Seon-Jeong (Dept. of Food Science & Technology, Korea National University of Transportation) ;
  • Choi, Ung-Kyu (Dept. of Food Science & Technology, Korea National University of Transportation)
  • 최원석 (한국교통대학교 식품공학과) ;
  • 최선정 (한국교통대학교 식품공학과) ;
  • 최웅규 (한국교통대학교 식품공학과)
  • Received : 2013.03.21
  • Accepted : 2013.06.07
  • Published : 2013.06.30

Abstract

This study was designed to investigate changes in germination characteristics and color as well as the content of isoflavones of Eunhakong germinated under dark conditions. The wet weight of Enhakong was increased as germination progressed. It reached $299.7{\pm}15.0%$ at 60 h after the beginning of sprouting. The relative dry weight was consistently decreased as germination progressed, and it reached $65.8{\pm}8.2%$ at 60 h after the beginning of sprouting. The length of sprout was rapidly increased at the latter stage compared to the beginning of germination. Most soybean seeds were successfully germinated at 60 h after beginning of sprouting. The L value was considerably increased at the early stage of germination and it reached $63.4{\pm}0.7$ at 60 h. The a value was rapidly increased at the beginning of germination and decreased thereafter. The b value was increased at the beginning of germination and did not changed thereafter. It was confirmed that the total isoflavone content, in which the m-genistin was highest followed by daidzin, and than slightly decreased.

본 연구에서는 암조건에서 은하콩의 발아시 발아 특성과 색도 변화 및 아이소플라본 함량의 변화를 확인하였다. 발아수율은 발아 시간이 점차 증가함에 따라 수율도 높아져서 발아 60시간째에는 $299.7{\pm}15.0%$를 나타내었다. 건조 중량은 발아가 진행됨에 따라 지속적으로 감소하여 발아 60시간째에는 $65.8{\pm}8.2%$를 나타내었다. 뿌리길이는 발아 초기에서 보다는 발아 후기에 급격한 증가 패턴을 보이는 것으로 확인되었다. 대부분의 콩은 발아 60시간 후에는 발아가 이루어진 것을 확인할 수 있었다. L값은 발아 12시간째에 비교적 높은 폭으로 증가하였으며, 발아 60시간째에 $63.4{\pm}0.7$까지 상승하는 것으로 확인되었다. a값은 $-2.6{\pm}0.3$으로 나타났으며, 발아 초기에 a값은 급격히 증가하였다가 이후 감소하는 패턴을 보였다. 수침한 콩의 b값은 발아 12시간째에 큰 폭으로 증가하였다가 그 이후는 큰 변화가 없는 것으로 확인되었다. 은하콩의 총 아이소플라본 함량은 발아 12시간째에 급격히 증가한 후 감소하였으며, 48시간까지는 발아시키지 않은 콩에 비해서는 높은 함량을 유지하였다. 함량별로는 m-genistin > daidzin > m-daizin > genistein의 순이었다.

Keywords

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