• The Korean Journal of Physiology and Pharmacology
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• v.15 no.3
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• pp.123-128
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• 2011

Caesalpinia sappan (C. sappan) is a medicinal plant used for promoting blood circulation and removing stasis. During a screening procedure on medicinal plants, the ethylacetate extract of the lignum of C. sappan (CLE) showed inhibitory activity on arginase which has recently been reported as a novel therapeutic target for the treatment of cardiovascular diseases such as atherosclerosis. CLE inhibited arginase II activity prepared from kidney lysate in a dose-dependent manner. In HUVECs, inhibition of arginase activity by CLE reciprocally increased NOx production through enhancement of eNOS dimer stability without any significant changes in the protein levels of eNOS and arginase II expression. Furthermore, CLE-dependent arginase inhibition resulted in increase of NO generation and decrease of superoxide production on endothelium of isolated mice aorta. These results indicate that CLE augments NO production on endothelium through inhibition of arginase activity, and may imply their usefulness for the treatment of cardiovascular diseases associated with endothelial dysfunction.

• Journal of Korean Medical classics
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• v.25 no.1
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• pp.141-161
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• 2012

Objective : In "Guanja" there is a clear system with xin and shengren as the central figure, which can be correlated with the united thought can be found in "Huangjenaegyeong". Method : In studying Oriental medicine, we will try to compare the Guanza and Huangjenaegyeong, in that what Sin is doing and how Seongin(聖人) does the work to achieve his prupose, and how Sin has philosophical meaning. Result : Guanja targets a unified government with the king centered, always issues an order, at the same time Guanja survey the system of the military, economy, retainer, people in the gross. In Guanja the Seongin is the ideal king, who understands the four seasons and command in timely, and rule his people using Muui(無爲). Sin is the center which achieve Dao(道) in mankind, and in the government system it is compared as the king to the rellation between king and retainer. "Huangjenaegyeong" is a medicine book which persued the united medical theory and algorithm. It made the general system algorithm, and united many different theories with the Seongin centered, who understand the 天氣 (including four seasons) and can control the human body. In this book, Sin is the center which control other organs. Conclusion : Political philoshphy in Guanja and the united thought in "Huangjenaegyeong" can be correlated in that two both aim for the systematization and the unity.

• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1295-1304
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• 2021

Modified Renshen Wumei decoction (MRWD), a famous traditional Chinese medicine, is widely used for treating persistent diarrhea. However, as the mechanism by which MRWD regulates diarrhea remains unknown, we examined the protective effects of MRWD on intestinal barrier integrity in a diarrhea model. In total, 48 male rats were randomly distributed to four treatment groups: the blank group (CK group), model group (MC group), Medilac-Vita group (MV group) and Chinese herb group (MRWD group). After a 21-day experiment, serum and colon samples were assessed. The diarrhea index, pathological examination findings and change in ᴅ-lactate and diamine oxidase (DAO) contents illustrated that the induction of diarrhea caused intestinal injury, which was ameliorated by MV and MRWD infusion. Metabolomics analysis identified several metabolites in the serum. Some critical metabolites, such as phosphoric acid, taurine, cortisone, leukotriene B4 and calcitriol, were found to be significantly elevated by MRWD infusion. Importantly, these differences correlated with mineral absorption and metabolism and peroxisome proliferator-activated receptor (PPAR) pathways. Moreover, it significantly increased the expression levels of TLR4, MyD88 and p-NF-κB p65 proteins and the contents of IL-1 and TNF-α, while the expression levels of occludin, claudin-1 and ZO-1 proteins decreased. These deleterious effects were significantly alleviated by MV and MRWD infusion. Our findings indicate that MRWD infusion helps alleviate diarrhea, possibly by maintaining electrolyte homeostasis, improving the intestinal barrier integrity, and inhibiting the TLR4/NF-κB axis.

• Bulletin of the Korean Chemical Society
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• v.33 no.9
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• pp.3079-3082
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• 2012

• Journal of Korean Medical classics
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• v.18 no.3 s.30
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• pp.116-125
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• 2005

1) Objective ${\lceil}Wai-Tai-Mi-Yao{\rfloor}$ had been made by Wang-Dao(王燾) in Tang Dynasty(唐朝). It included fumigation therapy and aroma therapy. Therefore we would like to bring out use sphere and detailed method of Fumigation therapy and Aroma therapy in Tang Dynasty and before period. 2) Conclusions (1) Fumigation therapies of ${\lceil}Wai-Tai-Mi-Yao{\rfloor}$ have contained boil and burn. The effects of fumigation therapy are made by cooperation effect of medicine and heat. (2) Aroma therapies of ${\lceil}Wai-Tai-Mi-Yao{\rfloor}$ have been used to cure infectious, internal, psychologic, dental, pediatric, dermatologic and surgical diseases. Especially these therapies have a good effect on cough. (4) Aroma therapies of ${\lceil}Wai-Tai-Mi-Yao{\rfloor}$ have been used to cure infectious, internal, psychologic, dental, ophthalmic, otolaryngologic, obstetrics, gynecologic, dermatologic and surgical diseases. Expecially this therapy has an good effect on nightmare.

• Korean Journal of Acupuncture
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• v.26 no.1
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• pp.1-25
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• 2009

Objectives : Medical qigong, originated from Mawangdui Daoyintu (馬王堆 導引圖), mainly consists of meditation, physical movements, and breathing exercises. It has been widely used to cure a variety of diseases as a regimen in Oriental medicine. This study was aimed to analyze the characteristics of medical qigong movements in the Mawangdui Daoyintu and to observe a link between each medical qigong movement and meridian system. Methods : We extracted fourteen medical qigong movements from forty-four figures in Mawangdui Daoyintu. We compared the 14 medical qigong movements of the Mawangdui Daoyintu with other types' movements of medical qigong methods. We also analyzed each movement of medical qigong in Mawangdui Daoyintu with a perspective of meridian system. Results : We found that there were common features between the medical qigong movements of the Mawangdui Daoyintu and other types' movements of medical qigong methods, including Yukjagyeol (六字訣), Paldangum (八段錦), Yukgengyeng (易筋經) and so on. From the comparison of each movement and meridian qi flow, we also found several movements related with Liver meridian and one movement related with several meridians. Conclusions : Our findings would be beneficial to understand the movements of medical qigong in the Mawangdui Daoyintu from the perspective of meridian system. This would be useful to develop a new medical qigong movements applying the meridian qi system for health and healing.

• Journal of Pharmacopuncture
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• v.16 no.2
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• pp.28-32
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• 2013

Objective: This study was performed to analyze the single-dose toxicity of D-amino acid oxidase (DAAO) extracts. Methods: All experiments were conducted at the Korea Testing & Research Institute (KTR), an institution authorized to perform non-clinical studies, under the regulations of Good Laboratory Practice (GLP). Sprague-Dawley rats were chosen for the pilot study. Doses of DAAO extracts, 0.1 to 0.3 cc, were administered to the experimental group, and the same doses of normal saline solution were administered to the control group. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results: In all 4 groups, no deaths occurred, and the $LD_{50}$ of DAAO extracts administered by IV was over 0.3 ml/kg. No significant changes in the weight between the control group and the experimental group were observed. To check for abnormalities in organs and tissues, we used microscopy to examine representative histological sections of each specified organ, the results showed no significant differences in any organs or tissues. Conclusion: The above findings suggest that treatment with D-amino acid oxidase extracts is relatively safe. Further studies on this subject should be conducted to yield more concrete evidence.

• BMB Reports
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• v.45 no.4
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• pp.247-252
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• 2012

Although previous studies have demonstrated that BMP9 is highly capable of inducing osteogenic differentiation of mesenchymal stem cells, the molecular mechanism involved remains to be fully elucidated. In this study, we showed that BMP9 simultaneously promotes the activation of Smad1/5/8, p38 and ERK1/2 in C3H10T1/2 cells. Knockdown of Smad4 with RNA interference reduced nuclear translocation of Smad1/5/8, and disrupted BMP9-induced osteogenic differentiation. BMP9-induced osteogenic differentiation was blocked by p38 inhibitor SB203580, whereas enhanced by ERK1/2 inhibitor PD98059. SB203580 decreased BMP9-activated Smads singling, and yet PD98059 stimulated Smads singling in C3H10T1/2 cells. The effects of inhibitor were reproduced with adenovirus expressing siRNA targeted p38 and ERK1/2, respectively. Taken together, our findings revealed that Smads, p38 and ERK1/2 are involved in BMP9-induced osteogenic differentiation. Also, it is noteworthy that p38 and ERK1/2 may play opposing regulatory roles in mediating BMP9-induced osteogenic differentiation of C3H10T1/2 cells.

• Korean Journal of Oriental Medicine
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• v.11 no.1
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• pp.43-60
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• 2005

Objective : ${\ulcorner}$Wai-Tai-Mi-Yao${\lrcorner}$ had been made by Wang-Dao(王燾) in Tang Dynasty(唐朝). It included not only in those days medical knowledge, but also before medical knowledge. So it is regarded as important classic in the oriental medicine. And there are various bathing therapy methods. Therefore we would like to bring out use sphere and detailed method of bathing therapy in Tang Dynasty and before period. Methodologies :We have researched bathing therapy of ${\ulcorner}$Wai-Tai-Mi-Yao${\lrcorner}$ according to below the procedure. (1) Making a related words list: We have used existing external treatments technical books to make a list. The list is consist of 23 words and includes not only technical terms, but also general terms. (2) Searching sentence: We have searched sentence that contain terms that related with bathing therapies. (3) Analysis of related sentence: We have searched and classified sentence by disease. Conclusions :(1) ${\ulcorner}$Wai-Tai-Mi-Yao${\lrcorner}$ has described 15,180 records. Bathing therapies of ${\ulcorner}$Wai-Tai-Mi-Yao${\lrcorner}$ had been used to cure 726 records from the whole volume. The contents account for 4.8% of the whole volume. (2) ${\ulcorner}$Wai-Tai-Mi-Yao${\lrcorner}$ has described 1,104 diseases. Bathing therapies of ${\ulcorner}$Wai-Tai-Mi-Yao${\lrcorner}$ had been used to cure 293 diseases from the whole diseases. The contents account for 26.5% of the whole volume. (3) These diseases belong to dermatologic, internal, ophthalmic, otolaryngologic, obstetrics, gynecologic, pediatric, surgical and veterinary diseases.

• Journal of Preventive Medicine and Public Health
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• v.53 no.4
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• pp.236-244
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• 2020

Objectives: Although the World Health Organization (WHO) initiative "My 5 Moments for Hand Hygiene" has been lauded as effective in preventing hospital-associated infections, little is known about healthcare workers (HCWs)' hand hygiene behavior. In this study, we sought to assess knowledge and attitudes towards the concepts in this initiative, as well as associated factors, among Vietnamese HCWs at a general hospital. Methods: A structured questionnaire was administered to HCWs at a central Vietnamese general hospital in 2015. Multiple logistic regression analysis was used to identify factors associated with HCWs' knowledge and attitudes towards hand hygiene. Results: Of 120 respondents, 65.8% and 67.5% demonstrated appropriate knowledge and a positive attitude, respectively, regarding all 5 hand hygiene moments. Logistic regression indicated better knowledge of hand hygiene in workers who were over 30 years old, who were direct HCWs (rather than managers), who had frequent access to clinical information, and who received their clinical information from training. Those who worked in infectious and tropical disease wards, who had frequent access to clinical information, and who received information from training were more likely to have a positive attitude towards hand hygiene than their counterparts. Conclusions: Although many Vietnamese HCWs displayed moderate knowledge and positive attitudes towards the WHO hand hygiene guidelines, a key gap remained. Regular education and training programs are needed to increase knowledge and to improve attitudes and practices towards hand hygiene. Furthermore, a combination of multimodal strategies and locally-adapted interventions is needed for sustainable hand hygiene adherence.