• Title/Summary/Keyword: Damage tolerance

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Antioxidative and Probiotic Properties of Lactobacillus gasseri NLRI-312 Isolated from Korean Infant Feces

  • Kim, H.S.;Jeong, S.G.;Ham, J.S.;Chae, H.S.;Lee, J.M.;Ahn, C.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.9
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    • pp.1335-1341
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    • 2006
  • We selected a Lactobacillus spp. from Korean healthy infant feces based upon their antioxidant activity. This strain was identified as Lactobacillus gasseri by 16S rDNA sequencing, and named Lactobacillus gasseri NLRI-312. In the present study, we investigate the protective effect of this strain on the $H_2O_2$ induced damage to cellular membrane lipid and DNA in Jurkat cells. To estimate the extent of cellular lipid peroxidation inhibition, MDA (malondialdehyde) was measured, and DNA damage was tested by the comet assay. We also examined probiotic properties including tolerance to acid and bile, antibiotic resistance. From the results obtained, the supplementation of Jurkat cells with NLRI-312 decreased in DNA damage, while no effect was shown on MDA decrease. In probiotic properties, this strain was resistance to both acid and bile, showed considerably higher survival when incubated in pH 2 or 1% bile salts (w/v). We concluded that the NLRI-312 could be used as potential probiotic bacteria, with the effect of reducing DNA damage induced by $H_2O_2$.

Residual Strength of Fiber Metal Laminates After Impact (충격손상을 받은 섬유 금속 적층판의 잔류 강도 연구)

  • Nam, Hyun-Wook;Lee, Young-Tae;Jung, Chang-Kyu;Han, Kyung-Seop
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.27 no.3
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    • pp.440-449
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    • 2003
  • Residual strength of fiber metal laminates after impact was studied. 3/4 lay up FML was fabricated using 4 ply prepreg, 2 ply aluminum sheets, and 1 ply steel sheet. Quasi isotropic ([0/45/90/-45]s) and orthotropic ([0/90/0/90]s) FRP were also fabricated to compare with FML. Impact test were conducted by using instrumented drop weight impact machine (Dynatup, Model 8250). Penetration load and absorbed energy of FML were superior to those of FRPs. Tensile tests were conducted to evaluate the residual strength after impact. Strength degradation of FML was less than that of FRP. This means that the damage tolerance of FML is excellent than that of FRP. Residual strength of each specimen was predicted by using Whitney and Nuismer(WN) Model. Impact damage area is assumed as a circular notch in WN model. Damage width is defined as the average of back face and top face damage width of each specimen. Average stress and point stress criterions were used to calculate the characteristic length. It is supposing that a characteristic length is a constant. The distribution of characteristic length shows that the assumption is reasonable. Prediction was well matched with experiment under both stress criterions.

Design and Analysis on Composite Structure for Aircraft Certification (항공기 인증을 위한 복합재 구조물 설계/해석)

  • Kim, Sung-Joon;Choi, Ik-Hyeon;Ahn, Seok-Min;Yeom, Chan-Hong
    • Aerospace Engineering and Technology
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    • v.8 no.1
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    • pp.42-48
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    • 2009
  • There are a number of factors affecting the continued airworthiness of composite structure. Unlike metal structure, damages made in manufacturing processes or maintenance repair procedures need to be considered. The different levels of degradation and damage, which may occur, must be considered for structural substantiation of static strength, stiffness, flutter, and damage tolerance. This can start with an evaluation of environmental effects for the particular composite material. Matrix-dominated composite properties, such as compressive strength, are most sensitive to moisture absorption and temperatures. Static strength substantiation includes the smaller damages that will not be detected in production or maintenance inspection while damage tolerance addresses larger damages that need to be repaired once discovered. In this paper, we intend to list the airworthiness regulations and advisory circular that are deemed closely related to the certification of composite airplanes.

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Effect of Nitric Oxide on Paraquat-Tolerance in Lettuce Leaves (상추잎의 Paraquat 내성에 미치는 Nitric oxide의 영향)

  • Lee, Jee-Na;Hong, Jung-Hee
    • Journal of Environmental Science International
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    • v.20 no.12
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    • pp.1509-1519
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    • 2011
  • The protective effect of nitric oxide (NO) on the antioxidant system under paraquat(PQ) stress was investigated in leaves of 8-week-old lettuce (Lactuca sativa L.) plants. PQ stress caused a decrease of leaf growth including leaf length, width and weight. Application of NO donor, sodium nitroprusside (SNP), significantly alleviated PQ stress induced growth suppression. SNP permitted the survival of more green leaf tissue preventing chlorophyll content reduction and of higher quantum yield for photosystem II than in non-treated controls under PQ exposure, suggesting that NO has protective effect on chloroplast membrane in lettuce leaves. Flavonoids and anthocyanin were significantly accumulated in the leaves upon PQ exposure. However, the rapid increase of these compounds was alleviated in the SNP treated leaves. PQ treatment resulted in lipid peroxidation and induced accumulation of hydrogen peroxide ($H_2O_2$) in the leaves, while SNP prevented PQ induced increase in malondialdehyde (MDA) and $H_2O_2$. These results demonstrate that SNP serves as an antioxidant agent able to scavenge $H_2O_2$ to protect plant cells from oxidative damage. The activities of two antioxidant enzymes that scavenge reactive oxygen species, superoxide dismutase (SOD) and catalase (CAT) in lettuce leaves in the presence of NO donor under PQ stress were higher than those under PQ stress alone. Application of 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), a specific NO scavenger, to the lettuce leaves arrested SNP mediated protective effect on leaf growth, photosynthetic pigment and antioxidant systems. However, PTIO had little effect on lettuce leaves under PQ stress compared with that of PQ stress alone. The obtained data suggest that the damage caused by PQ stress is in part due to increased generation of active oxygen by maintaining increased antioxidant enzyme activities and SNP protects plants from oxidative stress. From these results it is suggested that NO might act as a signal in activating active oxygen scavenging system that protects plants from oxidative damage induced by PQ stress and thus confer PQ tolerance.

Creating damage tolerant intersections in composite structures using tufting and 3D woven connectors

  • Clegg, Harry M.;Dell'Anno, Giuseppe;Partridge, Ivana K.
    • Advances in aircraft and spacecraft science
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    • v.6 no.2
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    • pp.145-156
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    • 2019
  • As the industrial desire for a step change in productivity within the manufacture of composite structures increases, so does the interest in Through-Thickness Reinforcement technologies. As manufacturers look to increase the production rate, whilst reducing cost, Through-Thickness Reinforcement technologies represent valid methods to reinforce structural joints, as well as providing a potential alternative to mechanical fastening and bolting. The use of tufting promises to resolve the typically low delamination resistance, which is necessary when it comes to creating intersections within complex composite structures. Emerging methods include the use of 3D woven connectors, and orthogonally intersecting fibre packs, with the components secured by the selective insertion of microfasteners in the form of tufts. Intersections of this type are prevalent in aeronautical applications, as a typical connection to be found in aircraft wing structures, and their intersections with the composite skin and other structural elements. The common practice is to create back-to-back composite "L's", or to utilise a machined metallic connector, mechanically fastened to the remainder of the structure. 3D woven connectors and selective Through-Thickness Reinforcement promise to increase the ultimate load that the structure can bear, whilst reducing manufacturing complexity, increasing the load carrying capability and facilitating the automated production of parts of the composite structure. This paper provides an overview of the currently available methods for creating intersections within composite structures and compares them to alternatives involving the use of 3D woven connectors, and the application of selective Through-Thickness Reinforcement for enhanced damage tolerance. The use of tufts is investigated, and their effect on the load carrying ability of the structure is examined. The results of mechanical tests are presented for each of the methods described, and their failure characteristics examined.

Rotational Wireless Video Sensor Networks with Obstacle Avoidance Capability for Improving Disaster Area Coverage

  • Bendimerad, Nawel;Kechar, Bouabdellah
    • Journal of Information Processing Systems
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    • v.11 no.4
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    • pp.509-527
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    • 2015
  • Wireless Video Sensor Networks (WVSNs) have become a leading solution in many important applications, such as disaster recovery. By using WVSNs in disaster scenarios, the main goal is achieving a successful immediate response including search, location, and rescue operations. The achievement of such an objective in the presence of obstacles and the risk of sensor damage being caused by disasters is a challenging task. In this paper, we propose a fault tolerance model of WVSN for efficient post-disaster management in order to assist rescue and preparedness operations. To get an overview of the monitored area, we used video sensors with a rotation capability that enables them to switch to the best direction for getting better multimedia coverage of the disaster area, while minimizing the effect of occlusions. By constructing different cover sets based on the field of view redundancy, we can provide a robust fault tolerance to the network. We demonstrate by simulating the benefits of our proposal in terms of reliability and high coverage.

Dose Effect of Phytosanitary Irradiation on the Postharvest Quality of Cut Flowers

  • Kwon, Song;Kwon, Hye Jin;Ryu, Ju Hyun;Kim, Yu Ri
    • Journal of People, Plants, and Environment
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    • v.23 no.2
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    • pp.171-178
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    • 2020
  • The present study was conducted to determine the effects of electron beam irradiation on the postharvest quality of cut flowers. Cut flowers were irradiated with electron beam at 100, 200, 400, 600, 800, 1,000, and 2,000 Gy with a 10 MeV linear electron beam accelerator to evaluate their irradiation tolerance. Postharvest quality was determined by monitoring fresh weight loss, flower longevity, flower diameter, flowering rate, visual quality of flowers and leaves, and chlorophyll content. Cut flowers showed a radiation-induced damage with increasing the irradiation dose. Flower longevity and fresh weight of cut flowers decreased when the irradiation dose was increased. Flower bud opening was also inhibited in a dose-dependent manner. The effective irradiation doses for 10% reduction of postharvest quality (ED10) values were 144.4, 451.6, and 841.2 Gy in the 'Medusa' lily, 'Montezuma' carnation, and 'Rosina White' eustoma, respectively. Although tolerance of cut flowers to electron beam irradiation vary according to species, cultivars, or maturity stage conditions, it is conceivable that 'Montezuma' carnation and 'Rosina White' eustoma could be tolerated and maintained overall postharvest quality up to 400 Gy, the generic irradiation dose approved by the Animal and Plant Health Inspection Service (APHIS) and the International Plant Protection Convention (IPPC) for postharvest phytosanitary treatments.

Simulation-Based Fault Analysis for Resilient System-On-Chip Design

  • Han, Chang Yeop;Jeong, Yeong Seob;Lee, Seung Eun
    • Journal of information and communication convergence engineering
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    • v.19 no.3
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    • pp.175-179
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    • 2021
  • Enhancing the reliability of the system is important for recent system-on-chip (SoC) designs. This importance has led to studies on fault diagnosis and tolerance. Fault-injection (FI) techniques are widely used to measure the fault-tolerance capabilities of resilient systems. FI techniques suffer from limitations in relation to environmental conditions and system features. Moreover, a hardware-based FI can cause permanent damage to the target system, because the actual circuit cannot be restored. Accordingly, we propose a simulation-based FI framework based on the Verilog Procedural Interface for measuring the failure rates of SoCs caused by soft errors. We execute five benchmark programs using an ARM Cortex M0 processor and inject soft errors using the proposed framework. The experiment has a 95% confidence level with a ±2.53% error, and confirms the reliability and feasibility of using proposed framework for fault analysis in SoCs.

A Study on the Ecological Carrying Capacity of Donggung and Wolji, Gyeongju - Centering around the Used Pattern and Damage Degree of Trail Routes - (경주 동궁과 월지의 생태적 수용력에 관한 연구 - 탐방로의 이용행태 및 손상도를 중심으로 -)

  • Li, Hong;Kang, Tai-Ho
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.6
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    • pp.70-78
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    • 2012
  • In order to calculate the ecological carrying capacity of Donggung and Wolji, Gyeongju, the number and time for various activities of tourists were surveyed and then the injury tolerance on lawns was predicted with seven types of various activities. According to the injury tolerance in various activities for the suitable carrying capacity, the normal carrying capacity and the limited carrying capacity were explicitly determined in four lawns. According to the number and time for various activities, the injury tolerance of various activities was predicted in four lawns, and the highest average injury tolerance was 2.15 in walking, the lowest average injury tolerance was 0.05 in lying. The highest injury tolerance was 1.31 in lawn 4, and then the order was 0.97 in lawn 3, 0.91 in lawn1, and 0.70 in lawn 2. The ecological carrying capacity was predicted with a damaged area according to the injury tolerance of various activities. The suitable carrying capacity was done separately to 40 persons, 249 persons, 107 persons, 37 persons, the limited carrying capacity was 116, 713, 306, 107 respectively and the normal carrying capacity was 75, 463, 198, 69 in four lawns. When managed in Donggung and Wolji, Gyeongju, the number of tourists was under the limited carrying capacity, the conformation would grow better.

Nucleus-DNA Damage and Different Response of Plant Cells to Paraquat in Relation to Enzyme Activity of Superoxide Dismutase. (Superoxide dismutase의 활성차이에 따른 식물세포의 paraquat에 대한 반응과 핵 DNA 손상 검정)

  • 권순태;이명현;오세명;정도철;김길웅
    • Journal of Life Science
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    • v.14 no.4
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    • pp.614-619
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    • 2004
  • This study was undertaken to investigate the different responses of cultured plant cells to paraquat treatment and nucleus-DNA damage in relation to enzyme activity of superoxide dismutase (SOD). Furthermore, this study was also carried out to understand the antioxidative mechanism of plant cells to environmental stress. We selected two different species of plant cultured cells, Ipomoea batatas as high-SOD species and Lonicera japonica as low-SOD species. The total activity and specific activity of SOD in a chlorophyllous cell of I. batatas were 3,736 unit/gㆍfresh weight and 547 unit/mgㆍprotein, respectively, and those in L. japonica were 23 unit/gㆍfresh weight and 13 unit/mgㆍprotein, respectively SOD activity in chlorophyllous I. batatas cells reached its maximum level at 10 to 15 days after subculture, whereas that in L. japonica remained at a very low SOD level during the whole period of subculture. In comparison to L. japonica, I. batatas, a high-SOD species, showed high tolerance to paraquat 10 and 50 mg/l treatment in terms of cell viability and electrolyte leakage. Based on the result of comet assay, the nucleus-DNA damage of two species by paraquat 50 mg/l treatment was not significantly different. However, I. batatas cells repaired their damaged DNA more effectively than the cells of the low-SOD species, L. japonica.