• Molecules and Cells
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• 제41권2호
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• pp.127-133
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• 2018

Chromatin remodeling factors are involved in many cellular processes such as transcription, replication, and DNA damage response by regulating chromatin structure. As one of chromatin remodeling factors, remodeling and spacing factor 1 (RSF1) is recruited at double strand break (DSB) sites and regulates ataxia telangiectasia mutated (ATM) -dependent checkpoint pathway upon DNA damage for the efficient repair. RSF1 is overexpressed in a variety of cancers, but regulation of RSF1 levels remains largely unknown. Here, we showed that protein levels of RSF1 chromatin remodeler are temporally upregulated in response to different DNA damage agents without changing the RSF1 mRNA level. In the absence of SNF2h, a binding partner of RSF1, the RSF1 protein level was significantly diminished. Intriguingly, the level of RSF1-3SA mutant lacking ATM-mediated phosphorylation sites significantly increased, and upregulation of RSF1 levels under DNA damage was not observed in cells overexpressing ATM kinase. Furthermore, failure in the regulation of RSF1 level caused a significant reduction in DNA repair, whereas reconstitution of RSF1, but not of RSF1-3SA mutants, restored DSB repair. Our findings reveal that temporal regulation of RSF1 levels at its post-translational modification by SNF2h and ATM is essential for efficient DNA repair.

• Journal of Ginseng Research
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• 제47권1호
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• pp.65-73
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• 2023

Background: Age-related macular degeneration (AMD) is a significant visual disease that induces impaired vision and irreversible blindness in the elderly. However, the effects of ginseng berry extract (GBE) on the retina have not been studied. Therefore, this study aimed to investigate the protective effects of GBE on blue light (BL)-induced retinal damage and elucidate its underlying mechanisms in human retinal pigment epithelial cells (ARPE-19 cells) and Balb/c retina. Methods: To investigate the effects and underlying mechanisms of GBE on retinal damage in vitro, we performed cell viability assay, pre-and post-treatment of sample, reactive oxygen species (ROS) assay, quantitative real-time PCR (qRT-PCR), and western immunoblotting using A2E-laden ARPE-19 cells with BL exposure. In addition, Balb/c mice were irradiated with BL to induce retinal degeneration and orally administrated with GBE (50, 100, 200 mg/kg). Using the harvested retina, we performed histological analysis (thickness of retinal layers), qRT-PCR, and western immunoblotting to elucidate the effects and mechanisms of GBE against retinal damage in vivo. Results: GBE significantly inhibited BL-induced cell damage in ARPE-19 cells by activating the SIRT1/PGC-1α pathway, regulating NF-kB translocation, caspase 3 activation, PARP cleavage, expressions of apoptosis-related factors (BAX/BCL-2, LC3-II, and p62), and ROS production. Furthermore, GBE prevented BL-induced retinal degeneration by restoring the thickness of retinal layers and suppressed inflammation and apoptosis via regulation of NF-kB and SIRT1/PGC-1α pathway, cleavage of caspase 3 and PARP, and expressions of apoptosis-related factors in vivo. Conclusions: GBE could be a potential agent to prevent dry AMD and progression to wet AMD.

• Journal of Digital Convergence
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• 제22권1호
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• pp.23-32
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• 2024

This study selected in-depth discharge damage survey data and analyzed 92,364 patients whose main diagnosis was S00-T98 (damage, addiction, and specific other results due to external factors) based on the Korean Standard Classification of Diseases and Deaths (KCD-7th) among patients discharged from the hospital after inpatient treatment from January 2016 to December 2018. As a result of analyzing the general characteristics of injured and traumatic patients, the incidence rate of men was higher in gender, and the incidence rate of women increased as the year increased. As a result of analyzing the characteristics of injury and trauma patients other than injury, the injury intention had a high rate of unintentional damage, the damage place was the highest on the road/road, and it showed a decreasing trend as the year increased, and it showed an increasing trend in the residential area. It can be used as basic data for the establishment of a related system to prevent damage as a result of subsang.

• Korean Journal of Acupuncture
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• 제20권2호
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• pp.101-120
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• 2003

This study is performed to investigate the cause, symptom and acupuncture on Dudurugi through the literature of oriental medicine. The findings of this study are as follows: 1. Dudurugi is caused by exogenous pathogenic factors(wind, heat, cold, damp), sthenic inter damage factors(heat accumulated in the intestine and stomach, blood-heat, blood-stasia) and asthenia inter damage factors(asthenia of the spleen and stomach, blood-asthenia, asthenia of energy-blood, yin-asthenia and blood-dryness, yan-asthenia and energy-asthenia). 2. The symptom of Dudurugi is appeared in the skin and its surface is processed apparently or itch. 3. The treatment of Dudurugi was used by expelling the wind with removing pathogen. 4. In the treatment of Dudurugi, The su-yangmyong taejang-kyong of 12 merdians was mainly used and, the kokchi(LI11) of acupoints was most used in the acupuncture and moxibustion. 5. In the treatment of Dudurugi, acupoints of tok-maek and chok-t'aeyangkyong were mainly used in the case of showing symptoms caused by exogenous pathogenic factors and, acupoints of chok-t'aemkyong were mainly used in the case of showing symptoms caused by damp-heat accumulated in the intestine and stomach. When there were any other symptoms accompanied, other acupoints were more used.

• Journal of the Korean Society of Environmental Restoration Technology
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• 제24권3호
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• pp.99-114
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• 2021

We examined a current status of damage in biodiversity and its causing factors in Hasidong Anin coastal dune, Gangneung-si, Gangwon province which is designated as ecological and landscape conservation area. In this study, we found that ecosystem and biodiversity have been primarily damaged by anthropogenic factors such as the construction of surrounding area, military facilities illegally dumped garbage and the expansion of windbreak forest. These factors occur to damage the landscape, ecosystem and biodiversity etc. There is a significant lack of basic data needed for preservation and restoration due to the lack of prior research and value assessment. In order to establish solutions for preservation and restoration, it is critical to collect fundamental data and implement value assessments. Therefore, further studies such as ecosystem services assessment, increasing biodiversity, spatial analysis and monitoring of various items related to coastal dunes are needed.

• Magazine of the Korea Concrete Institute
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• 제3권1호
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• pp.87-94
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• 1991

Many different damage models have been prolxlsed for concrete in the past. Most of these are not well suited to predict the residual strength of damaged RC members. This paper reviews some basic facts alxlut concrete damage and uses these to systematically model damage as a low-cycle fatigue phenomenon. Instead of the number of load cycles to failure the energy dissipation capacity of a member is taken as the main variable, which depends on many different factors. The model is capable of simulating reasonably well the strength and stiffness degradation of I{C members subjected to strong cyclic loads.

• Proceedings of the Korean Geotechical Society Conference
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• 한국지반공학회 2005년도 지반공학 공동 학술발표회
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• pp.330-335
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• 2005

Reliable predictions of the movement of earth retaining structures and the ground adjacent to braced walls in urban excavation are often difficult due to many variable factors. The ground settlement and the damage of adjacent structures in urban excavation has been an important issue. Therefore, the stability of the adjacent structures must be secured with the excavation support and research on the protection of adjacent structure is necessary. This study showed a countermeasure method for case of damage behavior in urban excavation.

• Journal of the Korean Institute of Educational Facilities
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• 제4권3호
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• pp.5-14
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• 1997

The purpose of this study is to present basic data in order to improve the efficiency and the objectivity of diagnosing educational structures built with the reinforced concrete. For achieving the purpose, this paper, firstly, researches damage aspects of 22 public educational of facilities in Seoul, and summarizes the results of that research. Through the analysis and the evaluation of damage aspects, this paper shows the conclusions as follows; (1) Main damage to reduce structural capacities of building is the differential settlement. (2) Though the steel corrosion is occurred by several factors, the main cause is the faulty construction. (3) To prevent the damage development, a proper repair strategy is very important.

• Structural Engineering and Mechanics
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• 제24권1호
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• pp.91-105
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• 2006

In this paper, post-earthquake capacity evaluation method of reinforced concrete buildings was studied. Substructure pseudo-dynamic test and static loading test of first story column in a four-story R/C building was carried out in order to investigate the validity of the evaluation method proposed in the Damage Assessment Guideline (JBDPA 2001). In pseudo-dynamic test, different levels of damage were induced in the specimens by pre-loading, and input levels of seismic motion, at which the specimens reached to the ultimate stage, were examined. From the experimental result, no significant difference in damage levels such as residual crack width between the specimens under static and pseudo-dynamic loading was found. It is shown that the seismic capacity reduction factors ${\eta}$ can provide a reasonable estimation of post-earthquake seismic capacity of R/C buildings suffered earthquakes.

• Journal of Forest and Environmental Science
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• 제24권3호
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• pp.137-142
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• 2008

In this paper, we apply a logistic regression model to the data of snow damage on sugi (Cryptomeria japonica) occurred in Toyama prefecture (in Japan) in 2004 for estimating the risk probability. In order to specify the factors effecting snow damage, we apply a model selection procedure determining optimal subset of explanatory variables. In this process we consider the following 3 information criteria, 1) Akaike's information criterion, 2) Baysian information criterion, 3) Bias-corrected Akaike's information criterion. For the selected variables, we give a proper interpretation from the viewpoint of natural disaster.