• Title/Summary/Keyword: Damage analysis

Search Result 7,769, Processing Time 0.051 seconds

Saprolegnia parasitica Isolated from Rainbow Trout in Korea: Characterization, Anti-Saprolegnia Activity and Host Pathogen Interaction in Zebrafish Disease Model

  • Shin, Sangyeop;Kulatunga, D.C.M.;Dananjaya, S.H.S.;Nikapitiya, Chamilani;Lee, Jehee;De Zoysa, Mahanama
    • Mycobiology
    • /
    • v.45 no.4
    • /
    • pp.297-311
    • /
    • 2017
  • Saprolegniasis is one of the most devastating oomycete diseases in freshwater fish which is caused by species in the genus Saprolegnia including Saprolegnia parasitica. In this study, we isolated the strain of S. parasitica from diseased rainbow trout in Korea. Morphological and molecular based identification confirmed that isolated oomycete belongs to the member of S. parasitica, supported by its typical features including cotton-like mycelium, zoospores and phylogenetic analysis with internal transcribed spacer region. Pathogenicity of isolated S. parasitica was developed in embryo, juvenile, and adult zebrafish as a disease model. Host-pathogen interaction in adult zebrafish was investigated at transcriptional level. Upon infection with S. parasitica, pathogen/antigen recognition and signaling (TLR2, TLR4b, TLR5b, NOD1, and major histocompatibility complex class I), pro/anti-inflammatory cytokines (interleukin $[IL]-1{\beta}$, tumor necrosis factor ${\alpha}$, IL-6, IL-8, interferon ${\gamma}$, IL-12, and IL-10), matrix metalloproteinase (MMP9 and MMP13), cell surface molecules ($CD8^+$ and $CD4^+$) and antioxidant enzymes (superoxide dismutase, catalase) related genes were differentially modulated at 3- and 12-hr post infection. As an anti-Saprolegnia agent, plant based lawsone was applied to investigate on the susceptibility of S. parasitica showing the minimum inhibitory concentration and percentage inhibition of radial growth as $200{\mu}g/mL$ and 31.8%, respectively. Moreover, natural lawsone changed the membrane permeability of S. parasitica mycelium and caused irreversible damage and disintegration to the cellular membranes of S. parasitica. Transcriptional responses of the genes of S. parasitica mycelium exposed to lawsone were altered, indicating that lawsone could be a potential anti-S. parasitica agent for controlling S. parasitica infection.

A Study on Optimization for Location and type of Dam Considering the Characteristic of Large Fault (대규모 단층특성을 고려한 최적 댐위치 및 형식 선정)

  • Kim, Han-Jung;Lyu, Young-Gwon;Kim, Young-Geun;Lim, Hee-Dae
    • Tunnel and Underground Space
    • /
    • v.22 no.4
    • /
    • pp.227-242
    • /
    • 2012
  • Youngju multipurpose dam is planned to minimizing the damage by flood and obtaining the water for industrial use in Nakdong river region. Faults in rock mass have strong influences on the behaviors of dam structure. Thus, it is very important to analyse for the characteristics of fault rocks in dam design. However, due to the limitation of geotechnical investigation in design stages, engineers have to carry out the additional geological survey including directional boring to find the distribution of faults and the engineering properties of faults for stability of dam. Especially, the selection of location of dam and type of dam considering fault zone must be analyzed through various experimental and numerical analysis. In this study, various geological survey and field tests were carried out to analyse the characteristics of the large fault zone through the complex dam is designed in foundation region. Also, the distribution of structural geology, the shape of faults and the mechanical properties of fault rock were studied for the reasonable design of the location and type of dam for long-term stability of the complex dam.

Improvement Effect of Stachys sieboldii MIQ. According to Mixing Ratio of Calcium on Memory Impairment in Scopolamine-induced Dementia Rats (칼슘 배합 비율에 따른 초석잠의 scopolamine 치매유도 흰쥐에 대한 기억손상 개선 효과)

  • Choe, Da-Jeong;Ahn, Hee-Young;Kim, Young-Wan;Kim, Tae-Hoon;Kim, Man-do;Cho, Young-Su
    • Journal of Life Science
    • /
    • v.26 no.7
    • /
    • pp.812-818
    • /
    • 2016
  • The aim of this study was to investigate the anti-amnesic effect of Stachys sieboldii MIQ. according to the mixing ratio of calcium on scopolamine-induced learning and memory impairment, in vivo. At the end of the adaptation period, SD rats were divided into a normal group (N), a control group (C: scopolamine), a positive control group (PC: scopolamine + tacrine), and a sample group (S: scopolamine + Stachys sieboldii MIQ., 1CS: scopolamine + low calcium-mixed Stachys sieboldii MIQ., 5CS: scopolamine + high calcium-mixed Stachys sieboldii MIQ.), and were tested with learning and memory tests. The C and CS groups were found to have a decreased scopolamine-induced memory deficit in the Y-maze and water maze tests. Brain tissue analysis showed that the CS group decreased acetylcholinesterase (AChE) activity and increased acetylcholine (Ach) content, both of which are indicative of neuronal cell activity. From a light microscopy study, the nucleus of neurons in the hippocampus of the brain was more shrunken or condensed in the C group compared to the CS group. In the CS group, the damage to the neurons in the hippocampus of the brain was suppressed. These results suggest that Stachys sieboldii MIQ. according to the mixing ratio of calcium provides a significant anti-amnesic effect against scopolamine-induced cholinergic system deficits and cognitive impairment.

Tat-Fused Recombinant Human SAG Prevents Dopaminergic Neurodegeneration in a MPTP-Induced Parkinson's Disease Model

  • Sohn, Eun Jeong;Shin, Min Jea;Kim, Dae Won;Ahn, Eun Hee;Jo, Hyo Sang;Kim, Duk-Soo;Cho, Sung-Woo;Han, Kyu Hyung;Park, Jinseu;Eum, Won Sik;Hwang, Hyun Sook;Choi, Soo Young
    • Molecules and Cells
    • /
    • v.37 no.3
    • /
    • pp.226-233
    • /
    • 2014
  • Excessive reactive oxygen species (ROS) generated from abnormal cellular process lead to various human diseases such as inflammation, ischemia, and Parkinson's disease (PD). Sensitive to apoptosis gene (SAG), a RING-FINGER protein, has anti-apoptotic activity and anti-oxidant activity. In this study, we investigate whether Tat-SAG, fused with a Tat domain, could protect SH-SY5Y neuroblastoma cells against 1-methyl-4-phenylpyridinium ($MPP^+$) and dopaminergic (DA) neurons in the substantia nigra (SN) against 1-methyl-4-phenyl-1,2,3,6-tetra-hydropyridine (MPTP) toxicity. Western blot and immunohistochemical analysis showed that, unlike SAG, Tat-SAG transduced efficiently into SH-SY5Y cells and into the brain, respectively. Tat-SAG remarkably suppressed ROS generation, DNA damage, and the progression of apoptosis, caused by $MPP^+$ in SH-SY5Y cells. Also, immunohistochemical data using a tyrosine hydroxylase antibody and cresyl violet staining demonstrated that Tat-SAG obviously protected DA neurons in the SN against MPTP toxicity in a PD mouse model. Tat-SAG-treated mice showed significant enhanced motor activities, compared to SAG- or Tat-treated mice. Therefore, our results suggest that Tat-SAG has potential as a therapeutic agent against ROS-related diseases such as PD.

Analysis of diversity of hemolytic microbiome from aquafarm of arkshell, Scapharca broughtonii (피조개 양식장 내 용혈성 미생물의 다양성 분석)

  • Gwon, Byeong-Geun;Kim, Young-Ok;Nam, Bo-Hye;Kim, Woo-Jin;Kong, Hee Jeong;Kim, Bong-Seok;Jee, Young-Ju;Lee, Sang-Jun;An, Cheul Min;Kim, Dong-Gyun
    • Journal of fish pathology
    • /
    • v.26 no.3
    • /
    • pp.193-206
    • /
    • 2013
  • The ark shell, Scapharca broughtonii is a marine bivalve mollusks belonging to the family Arcidae and important seafood for Korean and Japanese, and southern coast is brisk bays for the ark shell aquaculture. However, productivity of ark shell from these regions were rapidly reduced during the last decade due to mass mortality. The reason of this great damage has not yet been identified. To overcome this economic loss, diverse investigations were focused on environmental factors that affects in the physiology of S. broughtonii, but microbiological researches were performed insufficiently. Hemoglobin is one of the major blood component of ark shell and is damaged by some species of bacterial toxins. We concentrated on this red pigment because hemolysis could be the cause of ark shell mortality. In this study, we analyzed microbial diversity of underwater sediments in coastal regions and also existences in the body of S. broughtonii. We investigate about 4,200 isolates collected from June to September for microbial diversity of sediments and ark shell. We screened all of culturable microorganisms, and identified 25 genera 118 species, 24 genera 89 species, 30 genera 109 species and 39 genera 141 species, and selected 140 unique colonies for identification and challenge assay.

Protective Effect of Hericiumerinaceus Extracts on Hepatic Injury Induced by Benzo($\alpha$)pyrene in Mice (노루궁뎅이 버섯 추출물의 벤조피렌 유발 간 독성에 대한 보호효과)

  • 박선희;김지영;장종선;오은정;김옥미;배준태;김현정;하대중;이갑랑
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.30 no.5
    • /
    • pp.928-932
    • /
    • 2001
  • The protective effect of Hericium erinaceus methanol extract was investigated on benzo($\alpha$)pyrene(B($\alpha$)P) induced hepatotoxicity in mice, Hericium erinaceus extract was intraperitioneally injected once a hay for successive 5 days. followed by treatment with B($\alpha$)P on the fifth day. The elevated activities of serum aminotransferase and hepatic cytochrome P-450 by B($\alpha$)P was decreased by pretretament with Hericium erinaceus extract. Moreover, hepatic lipid peroxide content and glutathions S-transferase activity increased by B($\alpha$)P were significantly decrease, but depletion of glutathione content induced by B($\alpha$)P was prevented by Hericium erinaceus extract. In addition, the increased activities of superoxide diamutase, catalase and glutathions peroxidase after B($\alpha$)P-treatment were decreasd. Immunoblott analysis of hepatic microsomes showed that methanol extract of Hericium erinaceus suppressed protein level of the cytochrome P-450 1AI increaed by B($\alpha$)P. These results suggest that Hericium erinaceus extract may protect liver from damage induced by B($\alpha$)P.

  • PDF

Thermal Shock Properties of 316 Stainless Steel (316 스테인레스강의 열충격 특성)

  • Lee, Sang-Pill;Kim, Young-Man;Min, Byung-Hyun;Kim, Chang-Ho;Son, In-Soo;Lee, Jin-Kyung
    • Journal of Ocean Engineering and Technology
    • /
    • v.27 no.5
    • /
    • pp.22-27
    • /
    • 2013
  • The present work dealt with the high temperature thermal shock properties of 316 stainless steels, in conjunction with a detailed analysis of their microstructures. In particular, the effects of the thermal shock temperature difference and thermal shock cycle number on the properties of 316 stainless steels were investigated. A thermal shock test for 316 stainless steel was carried out at thermal shock temperature differences from $300^{\circ}C$ to $1000^{\circ}C$. The cyclic thermal shock test for the 316 stainless steel was performed at a thermal shock temperature difference of $700^{\circ}C$ up to 100 cycles. The characterization of 316 stainless steels was evaluated using an optical microscope and a three-point bending test. Both the microstructure and flexural strength of 316 stainless steels were affected by the high-temperature thermal shock. The flexural strength of 316 stainless steels gradually increased with an increase in the thermal shock temperature difference, accompanied by a growth in the grain size of the microstructure. However, a thermal shock temperature difference of $800^{\circ}C$ produced a decrease in the flexural strength of the 316 stainless steel because of damage to the material surface. The properties of 316 stainless steels greatly depended on the thermal shock cycle number. In other words, the flexural strength of 316 stainless steels decreased with an increase in the thermal shock cycle number, accompanied by a linear growth in the grain size of the microstructure. In particular, the 316 stainless steel had a flexural strength of about 500 MPa at 100 thermal-shock cycles, which corresponded to about 80% of the strength of the as-received materials.

Active Water-Level and Distance Measurement Algorithm using Light Beam Pattern (광패턴을 이용한 능동형 수위 및 거리 측정 기법)

  • Kim, Nac-Woo;Son, Seung-Chul;Lee, Mun-Seob;Min, Gi-Hyeon;Lee, Byung-Tak
    • Journal of the Institute of Electronics and Information Engineers
    • /
    • v.52 no.4
    • /
    • pp.156-163
    • /
    • 2015
  • In this paper, we propose an active water level and distance measurement algorithm using a light beam pattern. On behalf of conventional water level gauge types of pressure, float-well, ultrasonic, radar, and others, recently, extensive research for video analysis based water level measurement methods is gradually increasing as an importance of accurate measurement, monitoring convenience, and much more has been emphasized. By turning a reference light beam pattern on bridge or embankment actively, we suggest a new approach that analyzes and processes the projected light beam pattern image obtained from camera device, measures automatically water level and distance between a camera and a bridge or a levee. As contrasted with conventional methods that passively have to analyze captured video information for recognition of a watermark attached on a bridge or specific marker, we actively use the reference light beam pattern suited to the installed bridge environment. So, our method offers a robust water level measurement. The reasons are as follows. At first, our algorithm is effective against unfavorable visual field, pollution or damage of watermark, and so on, and in the next, this is possible to monitor in real-time the portable-based local situation by day and night. Furthermore, our method is not need additional floodlight. Tests are simulated under indoor environment conditions from distance measurement over 0.4-1.4m and height measurement over 13.5-32.5cm.

A New Malting Barley Variety, 'Baegho' with Resistance to Barley Yellow Mosaic Virus and Powdery Mildew (보리호위축병과 흰가루병 저항성 맥주보리 신품종 '백호')

  • Kim, Mi-Jung;Hyun, Jong-Nae;Park, Jong-Chul;Kim, Yang-Kil;Lee, Mi-Ja;Kim, Dae-Ho;Kang, Sung-Ju;Kim, Sung-Taeg
    • Korean Journal of Breeding Science
    • /
    • v.43 no.6
    • /
    • pp.538-542
    • /
    • 2011
  • A new malting barley variety, 'Baegho', was developed from a cross between Azuma Golden and Nishinochikara in 1998. An elite line, YMB2139-2B-1-10-2, was selected in 2005 and designated as Milyang137. It showed good agronomic performance in the regional adaptation yield trials (RYT) from 2006 to 2008 and was released with the name of 'Baegho' having high yielding and BaYMV and powdery mildew resistance. The average heading and maturing dates of 'Baegho' were April 21 and May 28, which were 3 and 2 day later than those of Hopum, check variety, at the RYT, respectively. 'Baegho' has shoter culm length (78 cm), more spikes ($1,062per\;m^2$) and heavier 1,000 grain weight (40.7 g) than those of Hopum, respectively. 'Baegho' is resistant to domestic BaYMV strains I, III and IV and powdery mildew at Iksan. In growth habit, 'Baegho' is close to type (IV) that is distinguished from other Korean malting barley cultivars, which could tolerant to premature heading damage that is major concern for the practice of the malting barley cropping. The yield potential of 'Baegho' in the RYT was about 5.57 MT/ha, which is about 13% higher than those of Hopum. This variety has good malting quality in grain and malting analysis showed better 1,000 grain weight, husk rate, diastatic power than those of Hopum. 'Baegho' applied for protection of new varieties of plant in 2009.

The Effects of Co-cultivation Medium and Culture Conditions on Rice Transformation Efficiency (공동배양과정의 배지조성과 배양조건이 벼 형질전환효율에 미치는 영향)

  • Kim, Yul-Ho;Park, Hyang-Mi;Choi, Man-Soo;Yun, Hong-Tai;Choi, Im-Soo;Shin, Dong-Bum;Kim, Chung-Kon;Lee, Jang-Yong
    • Korean Journal of Breeding Science
    • /
    • v.41 no.3
    • /
    • pp.252-260
    • /
    • 2009
  • Rice is the most important cereal crop not only in supplying the basic staple food for more than half of the world's population but also as a model plant for functional genomic studies of monocotyledons. Although rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5 mg/L L-cysteine, 1 mM sodium thiosulfate, 1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5 mg/L silver nitrate). Co-cultivation temperature ($23.5^{\circ}C$ for 1 day, $26.5^{\circ}C$ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.