• Asian-Australasian Journal of Animal Sciences
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• 제33권8호
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• pp.1265-1272
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• 2020

Objective: To evaluate the effect of feeding acidified milk on the growth and fecal microbial diversity of dairy calves. Methods: Twenty healthy 3-day-old female Holstein calves with similar body weights were selected and randomly divided into two groups. One group was fed pasteurized milk (PM, Control), while the other was fed acidified milk (AM) ad libitum until weaned (day 60). The experiment lasted until day 180. Results: There was no difference in the nutritional components between PM and AM. The numbers of Escherichia coli and total bacteria in AM were lower than in PM. At 31 to 40 and 41 to 50 days of age, the milk intake of calves fed AM was higher than that of calves fed PM (p<0.05), and the solid feed intake of calves fed AM was higher than that of calves fed PM at 61 to 90 days (p<0.05). The average daily gain of calves fed AM was also higher than that of calves fed PM at 31 to 60, 61 to 180, and 7 to 180 days (p<0.05). The calves fed AM tended to have a lower diarrhea rate than those fed PM (p = 0.059). Bacteroides had the highest abundance in the feces of calves fed AM on day 50, while Ruminococcaceae_UCG_005 had the highest abundance in the feces of calves fed AM on day 90 and calves fed PM on days 50 and 90. At the taxonomic level, the linear discriminant analysis scores of 27 microorganisms in the feces of calves fed AM and PM on days 50 and 90 were higher than 4.0. Conclusion: Feeding AM increased calf average daily gain and affected fecal bacterial diversity.

• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1711-1717
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• 2011

The objective of this experiment was to evaluate the effect of dietary supplementation of glutathione (GSH) on health, solid feed consumption, nutrient intake, body weight gain (BWG), feed efficiency, blood metabolites and the occurrence of diarrhea in Holstein neonatal calves. The calves were fed plain milk as a control (CON) or milk with GSH supplementation. Sixteen calves were separated from their mothers immediately after birth, moved into individual cages and fed colostrum for the first three days. For GSH supplementation, three grams of GSH powder were mixed in 1.8 L of heat-treated milk and placed in a plastic bottle with a rubber nipple. The calves were fed GSH-supplemented milk only once out of four daily feedings. For the first 25 d, calves were fed 1.8 L of milk four times per day. Milk feeding frequency was reduced to three times per day from days 26 to 30, followed by twice a day from days 31 to 44, and once a day from days 45 to 49, after which they were weaned at day 50. Body weight gain (BWG), feed consumption, and growth performance were monitored until day 70. The dietary supplementation of GSH had no effect on daily feed intake and growth performance in growing calves. Hematological results revealed red blood cell distribution width (RDW) was lower, and mean corpuscular volume (MCV) was significantly higher in calves fed GSH. Serum lactate dehydrogenase (LDH) concentrations were lower in calves fed GSH. Rectal temperature at day 70 was higher in calves that did not receive GSH, while mean frequency of diarrhea and enteritis was less in calves fed GSH. It is concluded from the present study that BW gain, total dry matter intake (DMI), feed efficiency, and breathing rate did not differ between groups. However, there were some positive blood parameters and the mean frequency of diarrhea and enteritis was less in calves fed GSH compared to CON which did not receive GSH. With the results obtained, supplementation of GSH is highly recommended.

• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.637-642
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• 2002

Thirty Holstein calves were used to determine effects of age, environment and sex on blood metabolite concentrations during 1 to 90 d of age. Calves were weaned at 75 d of age. Environmental effects are grouped by the difference in month at birth and site of feeding. Blood samples were obtained every 2 or 3 d. The mean metabolite concentration every 3 d was used for the statistical analysis. Dairy bodyweight gain was not affected by environmental group and sex effect. Concentrations of plasma glucose, nonesterified fatty acids (NEFA), triglyceride, total cholesterol and total ketone changed with growth. These developmental changes in metabolite levels would be caused by ruminal maturation with increment of grain intake. Levels of plasma urea nitrogen, glucose, NEFA, triglyceride and total cholesterol drastically changed during a few weeks after birth, indicating that the physiological state in calves greatly changed during that time. Effects of the environmental group and sex were significant in almost all metabolites. Temperature influenced plasma metabolite concentrations. The plasma metabolite concentrations were affected more intensely by heat stress in the infant period than in the neonatal period.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2001년도 발생공학 국제심포지움 및 학술대회 발표자료집
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• pp.37-43
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• 2001

1. About fifty thousand of cattle embryos were transferred and 16000 ET-calves were born in 1999. Eighty percents of embryos were collected from Japanese Black beef donors and transferred to dairy Holstein heifers and cows. Since 1985, we have achieved in bovine in vitro fertilization using immature oocytes Collected from ovaries of slaughterhouse. Now over 8000 embryos fertilized by Japanese Black bull, as Kitaguni 7 -8 or Mitsufuku, famousbulls as high marbling score of progeny tests were sold to dairy farmers and transferred to their dairy cattle every year. 2. Embryo splitting for identical twins is demonstrated an useful tool to supply a bull for semen collection and a steer for beef performance test. According to the data of Dr.Hashiyada (2001), 296 pairs of split-half-embryos were transferred to recipients and 98 gave births of 112 calves (23 pairs of identical twins and 66 singletons). 3. A blastomere-nuclear-transferred cloned calf was born in 1990 by a joint research with Drs.Tsunoda, National Institute of Animal Industry (NIAI) and Ushijima, Chiba Prefectural Farm Animal Center. The fruits of this technology were applied to the production of a calf from a cell of long-term-cultured inner cell mass (1998, Itoh et al, ZEN-NOH Central Research Institute for Feed and Livestock) and a cloned calf from three-successive-cloning (1997, Tsunoda et al.). According to the survey of MAFF of Japan, over 500 calves were born until this year and a half of them were already brought to the market for beef. 4. After the report of "Dolly", in February 1997, the first somatic cell clone female calves were born in July 1998 as the fruits of the joint research organized by Dr. Tsunoda in Kinki University (Kato et al, 2000). The male calves were born in August and September 1998 by the collaboration with NIAI and Kagoshima Prefecture. Then 244 calves, four pigs and a kid of goat were now born in 36 institutes of Japan. 5. Somatic cell cloning in farm animal production will bring us an effective reproductive method of elite-dairy- cows, super-cows and excellent bulls. The effect of making copy farm animal is also related to the reservation of genetic resources and re-creation of a male bull from a castrated steer of excellent marbling beef. Cloning of genetically modified animals is most promising to making pig organs transplant to people and providing protein drugs in milk of pig, goat and cattle.

• Asian-Australasian Journal of Animal Sciences
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• 제15권9호
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• pp.1267-1272
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• 2002

The present research was undertaken to monitor the effects of restricted and ad. libitum feeding during last trimester of pregnancy on the performance of crossbred dairy cows and their calves. For this purpose two groups of crossbred cows having eight animals in each group were put into two plane of nutrition during their last three months of pregnancy. Dietary energy level in one group was calculated as per Ministry of Agriculture, Fisheries and Food, (MAFF, UK) and the cows on other group received ad. libitum feeding (concentrate level similar to MAFF, recommendation+ad. libitum roughage). After calving, the cows of both groups were given same diet (ad. libitum) to study the effect of feeding during pregnancy on subsequent lactation performance of the cows. It was observed that during pre-calving period cows on ad. libitum feeding gained significantly (p<0.05) more body weight than that of cows on restricted feeding ($38.50{\pm}6.04$ vs $21.37{\pm}8.04kg/cow$). There was no significant differences between the birth weight of calves of restricted ($21.31{\pm}2.18kg$) and ad. libitum ($20.31{\pm}3.27kg$) groups. Dietary energy intake of ad. libitum group ($50.22{\pm}4.24$ MJ ME/d/cow) was significantly higher (p<0.01) than that of restricted group ($39.76{\pm}1.03$ MJ ME/d/cow). During lactation period cows that were on restricted feeding produced little more milk (3.06 lit/cow/day), than that of the cows on ad. libitum feeding (2.84 lit/cow/day). Although growth rate of the calves of restricted group upto one month ($0.23{\pm}0.09kg/calf/d$) was significantly (p<0.01) higher than that of ad. libitum group ($0.17{\pm}0.08kg/calf/d$) but after four months the growth rate of the calves of two groups become nearly similar ($0.35{\pm}0.02$ vs $0.37{\pm}0.03kg/calf/d$) and difference between them was non-significant. During lactation period cows of restricted group gained little weight but cows of ad. libitum group lost significantly (p<0.05) more weight. Per day metabolizable energy intake in both group during lactation period was nearly similar (67.54 vs 69.58 MJ ME). It is concluded that ad. libitum feeding during last trimester of pregnancy is neither economic for getting maximum milk yield nor for calf growth rate and MAFF, recommended level of dietary energy during that period could be applied on our pregnant crossbred dairy cows.

• Asian-Australasian Journal of Animal Sciences
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• 제21권8호
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• pp.1151-1158
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• 2008

This study was designed to examine the effects of different dietary protein levels on growth performance, nutrient utilization, amino acid (AA) digestibility and serum biochemical parameters of preruminant dairy calves. Fifteen healthy new-born calves were randomly allotted to three experimental groups and fed with different milk replacer that contained 18% (LP), 22% (MP) or 26% (HP) of protein. The results showed that final BW, net gain and ADG were significantly higher in the MP group than in LP and HP groups (p<0.05). In addition, the apparent digestibility of CP in the MP group was significantly higher than in the other two groups (p<0.05). The values of N intake and fecal N excretion were significantly increased following the increase of dietary protein content (p<0.05). However, in all three groups of animals, dietary protein content had no significant effect on urinary N concentration (p>0.05). BUN concentration, on the other hand, decreased as calves grew but increased following the increase of dietary CP content. Furthermore, no significant differences in digestibility of amino acids were observed among these three groups of animals (p>0.05). We concluded that calves fed with milk replacer containing 22% of protein had better growth performance and nutrient utilization as compared to animals treated with milk replacer containing either 18% or 26% of protein.

• Asian-Australasian Journal of Animal Sciences
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• 제12권1호
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• pp.68-76
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• 1999

A formal survey was carried out in Nharira-Lancashire areas located in Chivhu to assess the calf rearing systems practiced in smallholder dairy farming areas of Zimbabwe. A total of 47 farmers, collectively owning 305 cows and 194 calves of various breeds, participated in the survey. All the farmers allowed their calves to suckle their dams all day to obtain colostrum. The colostrums intake period was significantly (p < 0.05) shorter (5.2 vs 4.1 days) in the small scale commercial area (SSCA) compared to communal area (CA). Milk was first sold to the Nharira-Lancashire Milk Centre a day after the colostrum intake period ended. Most of the CA (91.3%) and SSCA (77.8%) farmers penned their cows and calves together at night during the colostrum intake period. Thereafter the calves were penned separate from their dams. After colostrum intake, two types of calf suckling systems were practised; twice a day suckling and twice a day then changed to once a day suckling. In both systems, suckling was allowed for 30 minutes after the cows had been hand milked. There was no significant (p < 0.05) difference in the mean weaning age of calves between the CA and SSCA (5.8 vs 5.4 months). The most common weaning method was through separation of the calves from the dams. The limitaitions to calf production in Chivhu were the prohibitively high costs of calf meals, poor feed resources during the dry season, a general lack of knowledge on calf rearing diseases and inappropriate calf housing.

• Parasites, Hosts and Diseases
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• 제47권4호
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• pp.417-420
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• 2009

Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dog is the definitive host for N. caninum and can infect dairy cattle. The aim of this study is to determine the prevalence of Neospora oocysts in feces of dogs from dairy farms. A total of 174 fecal samples was collected from 89 farm dogs and 85 household dogs during 2006 and 2008. Fecal samples of dogs were microscopically examined for detecting Hammondia Neospora-like oocysts (HNLO) by Mini $Parasep^{(R)}SF$ fecal parasite concentrator. HNLO were microscopically detected in 4 fecal samples (2.2%). The fecal samples with HNLO were examined by N. caninum-specific PCR. Two of the samples were positive for N. caninum. The 2 positive fecal samples were selected for inoculation to calves. Two inoculated calves were seronegative by ELISA for 4 months post-infection. This is the first report of finding N. caninum DNA in feces of farm dogs in Mashhad area, Iran.

• Animal Bioscience
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• 제37권2호
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• pp.253-260
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• 2024

Objective: The objectives of the present study were to evaluate the effects of once-a-day milk feeding on growth performance and routine behavior of preweaning dairy calves. Methods: At 22nd day of age, twenty-four Holstein calves were randomly assigned to one of two treatment groups (n = 12/treatment) based on milk feeding frequency (MF): i) 3 L of milk feeding two times a day; ii) 6 L of milk feeding once a day. The milk feeding amount was reduced to half for all calves between 56 and 60 days of age and weaning was done at 60 days of age. To determine the increase in weight and structural measurements, each calf was weighed and measured at 3 weeks of age and then at weaning. The daily behavioral activity of each calf was assessed from the 22nd day of age till weaning (60th day of age) through Nederlandsche Apparatenfabriek (NEDAP) software providing real-time data through a logger fitted on the calf's foot. Results: There was no interaction (p≥0.17) between MF and sex of the calves for routine behavioral parameters, body weight and structural measurements. Similarly, there was no effect of MF on routine behavioral parameters, body weight and structural measurements. However, the sex of the calves affected body weight gain in calves. Male calves had 27% greater total body weight and average daily gain than female calves. There was no effect of the sex of the calves on behavioral measurements. Collectively, in the current study, no negative effects of a once-a-day milk feeding regimen were found on routine behavioral and growth parameters of preweaning calves in group housing. Conclusion: Once-a-day milk feeding can be safely adopted in preweaning calves from 22nd day of age.

• 한국임상수의학회지
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• 제27권2호
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• pp.154-158
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• 2010

소에서 유전적 군 (herd)의 향상을 위한 유용한 도구는 보조 생식술 중 배아 이식 (embryo transfer)으로 알려져 왔다. 본 연구의 목적은 캐나다 유전 능력 상위 1% 이내의 젖소에서 생산된 냉동 배아를 수입하여, 국내 수란우군을 이용한 배아이식을 적용한 후, 송아지를 생산하는 것이다. 117번의 배아 이식이 이루어졌고, 그 중 70 마리에서 임신이 확인 되었으며, 총 57 마리의 송아지가 성공적으로 태어났다. 그들의 임신기간, 체중, 성별은 각각 $278.1{\pm}3.6$ 일 (범위: 271 에서 286일), $44.0{\pm}3.0\;kg$ (범위: 37 에서 49 kg) and 57.9 vs. 42.1% (수컷: 33, 암컷: 24) 이었다. 미세위성체검사를 통하여, 태어난 송아지는 모두 수입된 배아 유래임이 증명되었다. 결론적으로, 본 연구를 통하여 캐나다에서 수입된 배아로부터 살아있는 송아지를 성공적으로 얻을 수 있었으며, 이 송아지들은 앞으로 국내 낙농산업에 있어 종모우와 종빈우가 되는 좋은 유전자원이 될 것이다.