Browse > Article

Calves Derived from in Vivo Frozen-Thawed Embryos Collected from Canada Holstein Friesian Cows with High Genetic Background  

Lee, Won-You (Embryo Research Center, Seoul Dairy Cooperative)
Lee, Woo-Sung (Embryo Research Center, Seoul Dairy Cooperative)
Kim, Hyung-Jong (Embryo Research Center, Seoul Dairy Cooperative)
Kim, Bong-Han (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University)
Hong, So-Gun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University)
Lee, Byeong-Chun (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University)
Jang, Goo (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Veterinary Clinics / v.27, no.2, 2010 , pp. 154-158 More about this Journal
Abstract
Embryo Transfer (ET) is one of the assisted reproductive technologies and a useful tool for improving herds. The purpose of this study is to produce the calves using frozen embryos which were produced in the top one percent Holstein in Canada by ET. One hundred seventeen recipients were used for surrogate mothers and seventy cows were diagnosed to be pregnant. Fifty seven calves were born successfully and thirteen out of them failed to produce viable calves (abortion: 4, stillbirth: 9). Their gestational length, birth body weight and sex ratio for all the viable calves(n = 57) were $278.1{\pm}3.6$ days (range: from 271 to 286 days), $44.0{\pm}3.0\;kg$ (range: from 37 to 49 kg) and 57.9 vs. 42.1 % (male 33 and female 24), respectively. Microsatellite analysis confirmed that they were derived from frozen embryos. In conclusion, this study demonstrated that viable calves derived from frozen-thawed embryos from Canada were born by ET.
Keywords
frozen embryos; embryo transfer; calves; microsatellite;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Lim KT, Jang G, Ko KH, Lee WW, Park HJ, Kim JJ, Kang SK, Lee BC. Improved cryopreservation of bovine preimplantation embryos cultured in chemically defined medium. Anim Reprod Sci 2008; 103: 239-248.   DOI   ScienceOn
2 Merton JS, de Roos APW, Mullaart E, de Ruigh L, Kaal L, Vos PLAM, Dieleman SJ. Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry. Theriogenology 2003; 59: 651-674.   DOI   ScienceOn
3 Lohuis MM. Potential benefits of bovine embryo-manipulation technologies to genetic improvement programs. Theriogenology 1995; 43: 51-60.   DOI   ScienceOn
4 Martinez AG, Brogliatti GM, Valcarcel A, de las H. Pregnancy rates after transfer of frozen bovine embryos: a field trial. Theriogenology 2002; 58: 963-972.   DOI   ScienceOn
5 Callesen H, Liboriussen T, Greve T. Practical aspects of multiple ovulation-embryo transfer in cattle. Animal Reproduction Science 1996; 42: 215-226.   DOI
6 Son G, song S, Jeong W, Park C, Lee J, IK K. Factors Affecting on pregnancy rate of recipients following transfer of HanWoo embryo produced by in vitro. Journal of embryo transfer 2008; 23: 37-42.   과학기술학회마을
7 Spell AR, Beal WE, Corah LR, Lamb GC. Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle. Theriogenology 2001; 56: 287-297.   DOI   ScienceOn
8 Stringfellow D, Mahajan R, Smith. Manual of the International Embryo Transfer Society Third edition: American Society of Civil Engineers, 1998; 170.
9 Funk DA. Major advances in globalization and consolidation of the artificial insemination industry. J Dairy Sci 2006; 89: 1362-1368.   DOI   ScienceOn
10 Choi J, Jung K. In vivo embryo production and transfer in Korean Brindle. Journal of embryo transfer 2008; 28: 11-20.
11 Gordon I. Laboratory production of cattle embryos, Second edition: CABI publishing, 2003.
12 Hasler JF. The current status and future of commercial embryo transfer in cattle. Animal Reproduction Science 2003; 79: 245-264.   DOI
13 Kim S, Ryoo Z, Park Y. Effect of Estrus Synchronization Protocols and GnRH Treatments on the Pregnancy a nd Fetal Loss Rate after Transfer of Korean Native Cattle Embryos to Holstein Recipients. Journal of embryo transfer 2008; 23: 109-114.   과학기술학회마을
14 Lafri M, Ponsart C, Nibart M, Durand M, Morel A, Jeanguyot N, Badinand F, De Mari K, Humblot P. Influence of CIDR treatment during superovulation on embryo production and hormonal patterns in cattle. Theriogenology 2002; 58: 1141-1151.   DOI   ScienceOn
15 Lim KT, Jang G, Ko KH, Lee WW, Park HJ, Kim JJ, Lee SH, Hwang WS, Lee BC, Kang SK. Improved in vitro bovine embryo development and increased efficiency in producing viable calves using defined media. Theriogenology 2007; 67: 293-302.   DOI   ScienceOn
16 Lim KT, Lee BC, Kang SK, Hwang WS. Effects of protein source and energy substrates on the in vitro development of bovine embryos in a two-step culture system. J Vet Sci 2003; 4: 73-78.
17 Bo GA, Baruselli PS, Chesta PM, Martins CM. The timing of ovulation and insemination schedules in superstimulated cattle. Theriogenology 2006; 65: 89-101.   DOI   ScienceOn