• Journal of the Korean Chemical Society
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• v.37 no.5
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• pp.503-512
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• 1993

The purpose of this study was to investigate the retention behavior of phenols and to predict their retention in RPLC. The retention data of twenty-five phenols were measured on a $\mu-{Bondapak}\;C_{18}$ and a polymeric $C_{18}$ columns with methanol-water and acetonitrile-water as a mobile phase. From the observation of enthalpy-entropy compensation phenomenon, the following conclusions are drawn with regard to the retention mechanism: 1) the retention mechanism of nitrophenols in different from that of metheyl-and chlorophenols in both mobile phase; 2) in methanol-water mobile phase, the retention mechanism of methyl-and chlorophenols is consistent in the range of methanol-water composition; 3) on the other hand, in the case of acetonitrile-water mobile phase, the retention mechanism depends on the volume fraction of acetonitrile. It means that the retention mechanism can not be explained only by a simple interaction. Based on retention data as compared with two columns, it may be said that the hydrophobic interaction of phenols with polymeric $C_{18}$ column was greater than that with monomeric $C_{18}$ column. The equations for predicting the retention of phenols were derived by using hydrophobic substituent constant $(\pi)$ and the sum of Hammett's constant $(\sigma)$ and Taft's steric constant $(E_s)$.

• Journal of the Korean Chemical Society
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• v.37 no.5
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• pp.513-522
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• 1993

The purpose of this study is to optimize the selectivity of mobile phase solvents for separation of 25 phenols in reversed phase liquid chromatography and to accomplish the simultaneous preconcentration and separation of trace phenols from water samples. Phenols used in this study were classified into three groups, chloro-, methyl-, and nitrophenols. Quaternary solvent mobile phases were employed to improve the selectivity. Overlapping resolution maps(ORM) as a statistical simplex techniques was used to predict the optimum solvent system. Additional criterion such as pH and temperature were also investigated. In order to improve the resolution and decrease the analysis time, isoselective multisolvent gradient elution system was employed with ORM-Prism method. The simultaneous preconcentration and separation of trace phenols from water samples were performed by using XAD-2/Dowex 1-X8 tandem column. When the extraction efficiency was evaluated by sampling up to 1 L of distilled water, recovery of the phenols, except phenol, was above 90% and the limit of detection of the phenols was 5 ppb. The XAD-2/Dowex 1-X8 method was superior to C18 cartridge in terms of recovery and selectivity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.1
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• pp.171-180
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• 2007

The purpose of this study is to determine the possibility of using Flos Sophora japonica as natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of Flos Sophora japonica a were measured. The contents of carbohydrate, crude protein, crude lipid and ash are 67.76%, 19.87%, 4.61% and 7.76%. And the calories of Flos Sophora japonica Linne was 318.32 Kcal. Total dietary fiber was 25.35% of total carbohydrates. The percentages of water soluble dietary fiber to insoluble dietary fiber were 1.80 % and 23.56 %, respectively. The protein were contained total 18 different kinds of amino acids. The contents of non-essential and essential amino acids were 4,898.78mg and 5,953.51mg. The K was the largest mineral followed by Ca, P and Mg, which means Flos Sophora japonica Linne is alkali material. The contents of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids were 29.69%, 34.93% and 35.38%. Therefore, the amount of the total unsaturated fatty acid was higher than that of any other plant. The content of vitamin C in Flos Sophora japonica Linne was higher than that of any other plant, which suggest that it could increase blood elasticity. The content of rutin, which is responsible for capillary vessel permeability, was 22.60%. The contents of water soluble antioxidative materials in 1 mL of water-extracted Flos Sophora japonica Linne were 3.9 ${\mu}$g which is comparable to 1233.0 mmol of vitamin C in antioxidant effect. The general nutrients and other antioxidatant bioactive materials in Flos Sophora japonica Linne were also potential materials for good health food. It is expected that follow up study of Flos Sophora japonica Linne through developing processed food and evaluation of their functional properties would provide useful information as a source of medicinal foods.

• Journal of KIISE:Software and Applications
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• v.33 no.12
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• pp.1022-1030
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• 2006

The location awareness is an important part of many ubiquitous computing systems, but a perfect location system does not exist yet in spite of many researches. Among various location tracking systems, we choose the RFID system due to its wide applications. However, the sensed RSSI signal is too sensitive to the direction of a RFID reader antenna, the orientation of a RFID tag, the human interference, and the propagation media situation. So, the existing location tracking method in spite of using the particle filter is not working well. To overcome this shortcoming, we suggest a robust location tracking method with a double layered structure, where the first layer coarsely estimates a tag's location in the block level using a regression technique or the SVM classifier and the second layer precisely computes the tag's location, velocity and direction using the particle filter technique. Its layered structure improves the location tracking performance by restricting the moving degree of hidden variables. Many extensive experiments show that the proposed location tracking method is so precise and robust to be a good choice for implementing the location estimation of a person or an object in the ubiquitous computing. We also validate the usefulness of the proposed location tracking method by implementing it for a real-time people monitoring system in a noisy and complicate workplace.

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.9-19
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• 2016

Xylanases sourced from different bacteria have significantly different enzymatic properties. Therefore, studying xylanases from different bacteria is important to their applications in different fields. A potential xylanase degradation gene in Massilia was recently discovered through genomic sequencing. However, its xylanase activity remains unexplored. This paper is the first to report a xylanase (XynRBM26) belonging to the glycosyl hydrolase family (GH10) from the genus Massilia. The gene encodes a 383-residue polypeptide (XynRBM26) with the highest identity of 62% with the endoxylanase from uncultured bacterium BLR13. The XynRBM26 expressed in Escherichia coli BL21 is a monomer with a molecular mass of 45.0 kDa. According to enzymatic characteristic analysis, pH 5.5 is the most appropriate for XynRBM26, which could maintain more than 90% activity between pH 5.0 and 8.0. Moreover, XynRBM26 is stable at 37℃ and could maintain at least 96% activity after being placed at 37℃ for 1 h. This paper is the first to report that GH10 xylanase in an animal gastrointestinal tract (GIT) has salt tolerance, which could maintain 86% activity in 5 M NaCl. Under the optimum conditions, K_m, V_max, and k_cat of XynRBM26 to beechwood xylan are 9.49 mg/ml, 65.79 μmol/min/mg, and 47.34 /sec, respectively. Considering that XynRBM26 comes from an animal GIT, this xylanase has potential application in feedstuff. Moreover, XynRBM26 is applicable to high-salt food and seafood processing, as well as other high-salt environmental biotechnological fields, because of its high catalytic activity in high-concentration NaCl.

• Economic and Environmental Geology
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• v.6 no.2
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• pp.81-114
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• 1973

The purpose of the present study is to clarify the stratigraphy and geologic structure of the Great Limestone Series by means of study on fossil conodonts and detail investigation of geologic structure. In recent years very few geologists in Korea argue without confident evidences against the age and stratigraphy of the Great Limestone Series which have been rather well established previously in most parts of the regions although it is ambiguous and has not been studied in other areas. Five type localities in the Kangweon basin where the Great Limestone Series is well cropped out were chosen for the study. Total 26 genus and 66 species of conodont were identified from 290 samples collected and treated. From the study on conodonts the age of each formations of the Great Limestone Series has been determined as follows: The Great Limestone Series of Duwibong type Duwibong limestone: Caradocian (mid-Ord.) Jikunsan shale: Landeilian (mid-Ord.) Maggol limestone: Llanvirn-Llandeilian (mid-Ord.) Dumugol: Arenigian (Ord.) Hwajeol: Upper Cambrian The Great Limestone Series of Yeongweol type Mungok (Samtaesan) : Ordovician Machari: upper Cambrian The Great Limestone Series of Jeongseon type Erstwhile Jeongseon limestone: mid-Ord. The erstwhile Jongseon Limestone formation in Jeongseon district is separated into Hwajeol, Dongjeom, Dumudong, and Maggol formations which were cropped out repeatedly by folding and faulting, but Maggol is predominant in areal distribution. Yemi Limestone Breccia bed is not a single bed but distributed in several horizons so that it bears no stratigraphic significance. The limestone bed above Yemi Limestone Breccia, which was believed by some geologists to be much younger than Ordovician, is identified to be Maggol and its age is determined to be mid-Ordovician. Sambangsan formation in Yeongweol district was believed to be Cambrian age and lower horizon than Machari formation by Kobayashi, but C. M. Son believed that it might belong to later than Ordovician and lies above the Great Limestone Series of Yeongweol type. It was identified to be upper Cambrian and lies beneath the Machari formation and above the Daeki formation, the lower most horizon of the Great Limestone Series. The age of Yeongweol type Choseon system is contemporaneous with that of Duwibong type Choseon system. The difference in lithofacies is not due to lateral facies change, but due to the difference in its depositional environment. The Yeongweol type Choseon system is believed to be deposited in the small Yeongweol basin which was separated from the main Kangweon sedimentary basin. Judging from these facts it is definitely concluded that there exists no Gotlandian formation in the regions studied. Structurally the Kangweon basin comprises five basins and two uplifted areas. These structures were originated by at least two crustal movements, that is, Songrim disturbance of Triassic and Daebo orogeny of Jurasic age.

• Journal of Korean Society for Atmospheric Environment
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• v.29 no.5
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• pp.668-681
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• 2013

The Korean government announced a national mid-term target to reduce 30% of greenhouse gas (GHG) emissions from business-as usual (BAU) level by 2020 in a voluntary and independent manner. In this study, we examined the $CO_2$ mitigation potential and conducted an economic effect analysis of green living actions in households in Korea. We also proposed some ways to implement green life to achieve the national target. If green lifestyle takes root in households nationwide, $CO_2$ emission would be reduced to 27.3% of the emission in 2007. This would save the country about 4.93 trillion won per year and each household could save about 300,000 won per year, which accounts for about 0.5% of GDP (as of 2007). Considering the five-year plan for green growth to invest 2% of GDP in green growth every year, this would not only reduce the economic burden on households, industries and the country but also increase economic growth potential by reinvesting the saved resources into green growth. Heating and lighting would be the greatest contributor to GHG mitigation of green life in the residential sector. It means we could achieve the national goal by reducing unnecessary heating and lighting and using energy-saving electric home appliances. The implementation of green living actions would reduce a significant amount of greenhouse gas emissions, ultimately relieving the burden on businesses to reduce GHG emissions. And it is one of the most cost-effective mitigation tools in order to achieve the mid-term GHG mitigation goal.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.11
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• pp.1721-1728
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• 2007

An in vitro and a feeding trial were conducted to investigate the effect of xylanase supplementation on the feeding value of growing pig diets containing high proportions of Chinese double-low rapeseed meals (DLRM). Seven diets were formulated to meet NRC (1998) nutrient requirements. Diet 1 based on corn-soybean meal was used as positive control 1, and diet 2, a practical diet which incorporated a conventional level of Chinese DLRM (60 g/kg diet), as positive control 2. Diet 3 contained a higher level of DLRM (100 g/kg diet) as the negative control. Diet 3 plus xylanase at 0.10, 0.25, 0.50 and 0.70 g/kg diet created diets 4, 5, 6 and 7, respectively. The seven diets were incubated in triplicate with the in vitro two-stage enzyme incubation method to predict responses of diets to xylanase in terms of digestibility of dry matter (DM), crude protein (CP) and neutral detergent fibre (NDF). In vitro, the negative control had the lowest CP and NDF digestibility. Both DM and CP digestibility were increased (p<0.05) owing to xylanase supplementation either at 0.50 or 0.70 g/kg diet, and NDF digestibility was improved following xylanase addition at all of the test levels. There was a high linear correlation ($r^2>90$, p<0.05) between the activity concentration of the enzyme when transformed into its logarithmic value and in vitro digestibility coefficients of DM, CP or NDF. In the feeding trial, 112 crossbred pigs were randomly assigned to seven dietary treatments with 16 replicate pens of one pig each. An obvious dose effect on growth rate was observed ($r^2=0.79$, p<0.05) within the inclusion levels of xylanase. Compared with the negative control, xylanase addition at 0.70 g/kg diet resulted in significantly increased ADG (878 g/d vs. 828 g/d, p<0.05), and a tendency towards improved growth rate (868 g/d vs. 828 g/d, p = 0.10) was also observed following the inclusion of xylanase at 0.50 g/kg diet. It would appear that the nutrient utilization of corn and Chinese DLRM diets by pigs could be enhanced by an appropriate amount of xylanase addition. The in vitro and in vivo results suggested that the in vitro incubation method is feasible for predicting responses of pigs to exogenous enzymes and identifying those preparations that possess potential for improvement of the nutritive values of feedstuffs.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.7
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• pp.917-922
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• 2008

A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events that control ovulation. In this study, a 9,026-bp DNA sequence containing the full coding region, all 8 introns and part of the 5'and 3' untranslated region of the porcine ADAMTS1 gene was obtained. Analysis of the ADAMTS1 gene using the porcine radiation hybrid panel indicated that pig ADAMTS1 is closely linkage with microsatellite marker S0215, located on SSC13q49. The open reading frame of its cDNA covered 2,844 bp and encoded 947 amino acids. The coding region of porcine ADAMTS1 as determined by sequence alignments shared 85% and 81% identity with human and mouse cDNAs, respectively. The deduced protein contained 947 amino acids showing 85% sequence similarity both to the human and mouse proteins, respectively. Comparative sequencing of three pig breeds revealed one single nucleotide polymorphism (SNP) within exon 7 of which a G-C substitution at position 6006 changes a codon for arginine into a codon for proline. The substitution was situated within a PvuII recognition site and developed as a PCR-RFLP marker for further use in population variation investigations and association analysis with litter size. Allele frequencies of this SNP were investigated in seven pig breeds/lines. An association analysis in a new Qingping female line suggested that different ADAMTS1 genotypes have significant differences in litter size (p<0.01).

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.4
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• pp.471-477
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• 2008

The porcine PRKAG3 (RN) gene encodes the regulatory gamma subunit of adenosine monophosphate-activated protein kinase (AMPK), which is a good candidate gene affecting meat quality. In this study, the effects of two missense mutations A595G (Ile199Val) and G154A (Gly52Ser) in porcine PRKAG3 gene on meat quality traits were studied in M. Longissimus dorsi (LD), M. Semispinalis capitis (SC) and M. Biceps femoris (BF) from different populations of 326 pigs. The PRKAG3 alleles 199I, 199IV, 52S and 52G were identified with PCR-RFLPs and all genotypes - 199I/199I, 199I/199V, 199V/199V, 52S/52S, 52S/52G and 52G/52G - were found. The frequency of V allele was larger than that of I allele in all populations. I allele frequency was zero in Chinese Meishan pigs (population D) especially. G allele frequency was larger than that of S allele in all populations except Large White (population A). Both variations at the PRKAG3 locus significantly affected these meat quality traits. The pork meat quality has not previously been established in Meishan or crosses thereof. The results suggested that generally pH of LD, SC and BF was higher in Meishan pigs than that in other populations. Moreover, Meishan pigs showed higher water-holding capacity and intramuscular fat (IMF), lower water content and water loss percentage compared to other populations in terms of the two variations. The results present here supply new evidence that alleles V199I and G52S at the PRKAG3 locus affect pork meat quality and provide useful information on pork production.