• 한국동물위생학회지
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• 제40권2호
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• pp.107-117
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• 2017

This study was conducted to investigate of hepatoprotective effect of dandelion water extract (DWE) according to repeated administration of thioacetamide (TAA) induced hepatotoxicity in Spraque-Dawley rats. Thirty rats were randomly assigned to 5 groups; normal control, DWE-control, TAA-control (TAA injection during the feeding of normal diet), TAA&DWE600 (TAA repeated injection during the feeding of DWE 600 mg/kg BW), TAA&DWE1200 (TAA repeated injection during the feeding of DWE 1,200 mg/kg BW). Rats in DWE-control and TAA&DWE groups were treated with DWE (600 or 1,200 mg/kg BW daily) by gavage for 20 days (twice a day). All the rats in the TAA-control and TAA&DWE groups were repeated injection of TAA (100 mg/kg BW) into the abdominal cavity 3 days interval and 12 hrs later, all rats were sacrificed. At the same time, normal control and DWE-control groups were injected normal saline. In TAA&DWE groups, serum alanine and aspartate aminotransferase (ALT, AST) were significantly decreased and triglyceride (TG) synthesis was significantly increased compared to TAA group. As well as total billilubin and GGT were slightly decreased by the treatment of DWE. Lipid peroxidation (MDA) concentration was significantly decreased and hepatic GSH content was slightly or significantly increased in the TAA&DWE groups compared to TAA group. Hepatic anti-oxidative enzyme activities, such as GSH, GST, SOD and catalase were slightly or significantly elevated by the treatment of DWE. According to these results, When dandelion extract was long term supplied, it could be used as a potential protective material for a longer time liver damage by repeated adminstration of the TAA.

• 한국동물위생학회지
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• 제36권4호
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• pp.233-242
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• 2013

The genus Taraxacum, known by the common name Dandelion, is a medicinal herb in the family Asteraceae. It has been traditionally used as a folk medicine for the treatment or prevention of various diseases due to its anti-inflammatory and anti-oxidative properties. In this study, we attempted to evaluate protective effects of Dandelion related with anti-oxidative activity to Thioacetamide (TAA)-induced liver damage. 36 rats were randomly assigned to six experimental groups : Control, Dandelion water extract (DWE), TAA, TAA&DWE 300, TAA&DWE 600, TAA&DWE 1,200 groups. Rats in DWE and TAA&DWE groups were pretreated with DWE (300, 600 or 1,200 mg/kg BW) by gavage for 7 days. All rats were treated intraperitoneally with TAA (200 mg/kg BW) or normal saline at 12 hours after last oral administration and sacrificed at 12 hours after last treatment. Levels of WBC and Neutrophil count were significantly decresed in TAA&DWE 1,200 group compared with that in TAA group (P<0.05). In TAA&DWE 600 and TAA&DWE 1,200 groups, serum AST, ALT, GGT levels were lower than TAA group (P<0.05). The serum TG level was significantly elevated in TAA&DWE groups compared with those in TAA group. Liver tissues from TAA group showed extensive histopathological changes, characterized by moderate or severe hepatocytes degeneration, inflammatory cell infiltration, and congestion. In the TAA&DWE group, The severity of histopathological lesions were decreased compared to those in the TAA group. The MDA concentration was significantly decreased and GSH content was significantly increased in the TAA&DWE 1,200 group compared to those in the TAA group. GR, CAT and GST activities in the TAA&DWE 1,200 group were significantly increased compared to those in the TAA group.

• 대한가정학회지
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• 제49권3호
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• pp.113-121
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• 2011

This study developed a method of calculating dynamic wearing ease (DWE) to make patterns for coverall work clothing. The DWE was measured from 3D body scan data of women in their 20's (n = 10). The body postures adopted for measuring the DWE were sitting and deep bending postures. Three types of experimental patterns (A, B, C) were developed. The DWE was applied at the waist and hip lines of the pattern. The location and size of the DWE varied from one pattern to another. For pattern B, DWE of 8.8cm was applied at the back of the waistline. For pattern C, 5.3cm was applied at the waist for the center back line, and 3.5cm was applied at the under hip level. The comfort of the experimental clothing was evaluated in six body postures. The results showed that patterns B and C were comfortable at the crotch, shoulders, and arm pits. Pattern C was comfortable at the hip and crotch areas in the arms lifting-up posture and the stepping-up posture. The appearance and fit of the experimental clothing were evaluated by the panels. Patterns B and C fitted better than pattern A at the abdomen. The results suggest that DWE should be applied at the waist and hip levels for making coverall work clothing patterns.

• 한국식품영양과학회지
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• 제37권2호
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• pp.177-183
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• 2008

본 연구에서는 실험동물에 민들레 열수추출물 식이를 급여한 후, GalN으로 간손상을 유발함으로써 그 예방효과를 혈액중의 생화학적 변화 및 간조직의 효소적인 변동을 통해서 규명하고자 하였다. GalN의 투여로 현저히 증가하였던 AST, ALT의 활성은 민들레 열수추출물 투여로 억제되었으나 군간의 차이는 보이지 않았고 ALP의 활성과 TBARS 함량은 3%의 추출물을 급여한 군에서 유의적인 감소를 보였다. GalN의 투여로 현격하게 높아졌던 혈중 $TNF-{\alpha}$의 농도 또한 감소하는 경향을 확인하였으나 유의적인 차이는 보이지 않았다. GalN의 투여로 억제되었던 catalase, GSH-reductase, Mn-SOD의 활성은 민들레 추출물 투여로 유의적인 회복이 관찰되었으나 GSH-px의 활성은 그 경향만을 확인할 수 있었다. 조직 검경을 통해 민들레 열수추출물의 간염 예방효과를 확인한 결과 GalN으로 인해 유발된 광범위한 간세포의 괴사와 변성, 지방변성 등이 민들레 열수추출물식이로 다소 감소하는 것을 관찰할 수 있었다. 이상의 결과로 민들레 열수추출물은 AST, ALT와 ALP의 활성 및 산화적 스트레스를 감소시키고 활성산소 해독계에 관여하는 효소의 활성을 증가시킴으로써 GalN으로 인한 간 손상을 예방하는 것으로 사료된다.

• 생명과학회지
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• 제28권12호
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• pp.1461-1468
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• 2018

알래스카 산 대구(Gadus chalcogrammus)는 북태평양에 분포하는 대구과 한류 어종이다. 알래스카 산 대구의 명란 원료, 프리미엄 고추장 명란, 프리미엄 발효 명란을 증류수와 에탄올로 추출하여 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+), 철 환원 항산화능(Ferric Reducing Antioxidant Potential, FRAP), 총 페놀함량을 평가하였다. 명란 원료의 증류수와 에탄올 추출물의 ABTS+ 소거능은 1.0 mg/ml 농도에서 각각 50.1%, 53.1%였다. 고추장 명란의 증류수와 에탄올 추출물의 ABTS+ 소거능은 1.0 mg/ml 농도에서 각각 71.3%, 71.6%였다. 발효 명란의 증류수와 에탄올 추출물의 ABTS+ 소거능은 1.0 mg/ml 농도에서 각각 68.7%, 70.4%였다. 명란원료의 증류수와 에탄올 추출물의 ABTS+에 대한 최소 저해 농도 값($EC_{50}$)은 각각 12.49 ug/ml과 12.21 ug/ml이었다. 고추장 명란의 증류수와 에탄올 추출물의 FRAP에 대한 최소 저해 농도 값($EC_{50}$)은 각각 10.67 ug/ml과 10.56 ug/ml이었다. 발효 명란의 증류수와 에탄올 추출물의 총 페놀 함량에 대한 최소 저해 농도 값($EC_{50}$)은 각각 10.45 ug/ml과 10.31 ug/ml이었다. Gallic acid를 대조구로 할 때 총 페놀 함량에 대한 상대적 활성은 명란 원료가 52.0%, 고추장 명란이 61.1%, 발효 명란이 63.6%였다. 명란 원료보다 고추장 명란과 발효 명란에서 ABTS+, FRAP 소거능 및 총 페놀 함량이 우수하였다.

• 대한본초학회지
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• 제20권4호
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• pp.53-60
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• 2005

Objectives : This study was carried out to determine if Dendrobii Herba have protective effect against cell injury induced by various toxic agents in rat kidney slices. Water(DWe) and methanol(DMe) extracts were prepared for this experiment. Methods : Cell injury was estimated by measuring lactate dehydrogenase(LDH). Lipid peroxidation was examined by measuring malondialdehyde, a product of lipid peroxidation. Results : DMe prevented the LDH release by $CCl_4$, menadione, tert-butyl hydroperoxide and mercury treatment in vitro in kidney slices, but DWe prevented the LDH release by $CCl_4$ and mercury. DMe also prevented reduction in GSH and lipid peroxidation induced by $CCl_4$ and mercury. Conclusion : Thus, DMe may have more powerful efficacy on anti-oxidative effects when compared with DWe. And further studies have to be followed concerned with extraction of Dendrobii Herba and its change of effects.

• 복식문화연구
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• 제19권5호
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• pp.1019-1030
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• 2011

The aim of this study was to develop a pattern of flight attendant uniform shirts to provide better comfort for their work postures. Flight attendants' work postures were evaluated to determine the problems of clothing and mobility during their work. The pattern of the flight attendants' uniform shirt was altered by applying dynamic wearing ease(DWE). DWE was calculated from four standardized dynamic postures and a static posture. An experimental garment was made with the altered postures. The researcher redesigned the pattern of the uniform shirts, which minimizes physical limitations in movements. The fit and mobility of the shirts were evaluated. Results of this study are as follows. First, the five representative work postures were selected by "clothing stress" and "repetitiveness." These postures included raised arms, twisting midriff and shoulder postures. Five representative postures were selected by using the ergonomic posture assessment device index(OWAS). Second, the experimental garment was developed by applying DWE across the back and at armhole depth, back length, and side length. Third, the fit and mobility of the experimental garments and the original uniform shirts were compared at the flight working environment set and 5 dynamic body postures of raising arms. The experimental garments made with an altered pattern provided better fit and mobility than the original sample shirts.

• 생명과학회지
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• 제29권4호
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• pp.428-435
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• 2019

알래스카 산 대구(Gadus chalcogrammus)는 대구과(family Gadidae)의 해산어종이다. 알래스카 명란 원료, 고추장 명란, 발효 명란을 증류수와 에탄올로 추출하여 2, 2- diphenyl - 1- picrylhydroazyl (DPPH), 하이드록시 라디칼(OH), 산화질소(NO)의 항산화 소거능을 평가하였다. 철 이온 환원력(FRAP)을 조사하였다. ABTS+ 소거능은 Brand-Williams 등의 방법에 따랐다. 고추장 명란의 증류수 추출물의 DPPH 소거능은 1.0 mg/ml 농도에서 71.9%였으며 에탄올 추출물에서 소거능은 73.7%였다. 발효 명란의 증류수 추출물의 DPPH 소거능은 1.0 mg/ml 농도에서 78.0%였고 에탄올 추출물에서 소거능은 78.4%였다. 명란 원료의 증류수 추출물과 에탄올 추출물의 DPPH에 대한 최소 저해 농도 값($IC_{50}$)은 각각 11.65 ug/ml과 11.47 ug/ml이었다. 명란 원료, 고추장 명란, 발효 명란의 에탄올 추출물의 OH 소거능은 1.0 mg/ml 농도에서 70.9%, 79.0, and 80.6%였다. 명란 원료의 증류수 추출물과 에탄올 추출물의 NO의 최소 저해 농도 값은 11.45 ug/ml과 11.41 ug/ml였다. 본 연구 결과, 고추장 명란과 발효명란은 원료 명란 보다 높은 DPPH, OH, NO 소거능을 나타내었다. 고추장 명란과 발효 명란은 자연 라디칼 소거능이 있어 식품으로써 항산화 보충제가 될 수 있을 것이다.

• 한국식품과학회지
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• 제44권5호
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• pp.607-612
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• 2012

본 연구는 민들레에 각각 30, 50% 당을 첨가하고 15-20에서 120일간 발효를 하여 30% 당을 첨가하여 발효시킨 민들레 발효물(FD30), 50% 당을 첨가하여 발효시킨 민들레 발효물(FD50)로 진행되었다. 총 페놀성 화합물 함량은 FD30, FD50이 각각 $78.9{\pm}2.17$, $59.35{\pm}2.56$ mg/g으로 DWE(dandelion water extract)의 $54.2{\pm}1.49$ mg/g 보다 높은 결과를 나타냈다. $IC_{50}$으로 나타낸 DPPH 라디칼 소거활성은 FD30이 $118.7{\pm}2.03{\mu}g/mL$, FD50이 $123.40{\pm}2.15{\mu}g/mL$의 결과를 나타냈고 FD30이 높은 항산화 효과를 보인다는 것을 확인했다. 항염활성을 보기 위해 실시한 NO 소거능은 FD30, FD50 모두 2 mg/mL 농도로 처리했을 때 $8.8{\pm}1.72$, $11.8{\pm}2.87{\mu}M$, 같은 농도의 민들레추출물은 $4.9{\pm}1.20{\mu}M$를 나타냈다. 효소활성능은 FD50이 탄수화물 분해, 단백질 분해, 지질 분해에서 높은 활성을 보였다. 혈전용해 활성은 FD30, FD50이 각각 $0.56{\pm}0.28$, $1.39{\pm}0.20$ unit/mg protein 활성을 보였으며 DWE는 0.28 unit/mg protein 의 활성을 나타냈다. 이 연구를 통해 당을 첨가하여 발효시킨 민들레 발효물의 항산화 및 항염 활성을 확인하였고, 여러 가지 유용한 효소활성을 입증하였다.

• 한국데이타베이스학회:학술대회논문집
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• 한국데이타베이스학회 1998년도 국제 컨퍼런스: 국가경쟁력 향상을 위한 디지틀도서관 구축방안
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• pp.448-474
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• 1998

Data warehouse (DW) is important for analytical processing. Metadata is a key to its architecture. This paper proposes an architecture that consists of seven components. To illustrate data warehouse environment (DWE), this Paper proposes taxonomies having four flows. on the basis or the taxonomies and metadata, this paper proposes a methodology for building the data warehouse and metadata simultaneously. This integrated development methodology (IDM) consists of seven phases: (i) preparatory phase, (ii) requirement analysis phase, (iii) data warehouse development phase, (iv) operational data store development phase, (v) data mart development phase, (vi) metastore development phase, and (vii) maintenance phase. A metastore system is Proposed to help develop metadata interactively. An illustrative example is investigated to demonstrate the usefulness of IDM.