• Title/Summary/Keyword: DUS test

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Effect of Dental Practicality Index training using an online video on decision-making and confidence level in treatment planning by dental undergraduates

  • Zhai Wei See;Ming Sern Lee;Abhishek Parolia;Shalini Kanagasingam;Shilpa Gunjal;Shanon Patel
    • Restorative Dentistry and Endodontics
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    • v.49 no.1
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    • pp.8.1-8.12
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    • 2024
  • Objectives: The purpose of this study was to evaluate the effect of Dental Practicality Index (DPI) training using an online video on the treatment planning decisions and confidence level of dental undergraduates (DUs). Materials and Methods: Ninety-four DUs were shown 15 clinical case scenarios and asked to decide on treatment plans based on 4 treatment options. The most appropriate treatment plan had been decided by a consensus panel of experienced dentists. DUs then underwent DPI training using an online video. In a post-DPI-training test, DUs were shown the same clinical case scenarios and asked to assign the best treatment option. After 6 weeks, DUs were retested to assess their knowledge retention. In all 3 tests, DUs completed the confidence level scale questionnaire. Data were analyzed using the related-samples Wilcoxon signed rank test and the independent-samples Mann-Whitney U test with the level of significance set at p < 0.05. Results: DPI training significantly improved the mean scores of the DUs from 7.53 in the pre-DPI-training test to 9.01 in the post-DPI-training test (p < 0.001). After 6 weeks, the mean scores decreased marginally to 8.87 in the retention test (p = 0.563). DPI training increased their confidence level from 5.68 pre-DPI training to 7.09 post-DPI training. Conclusions: Training DUs using DPI with an online video improved their decision-making and confidence level in treatment planning.

Legal Aspects for Quality Evaluation Standard of Plant Variety and Seed (식물 품종 및 종자의 품질평가 기준에 대한 규정현황)

  • 최근진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47
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    • pp.200-215
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    • 2002
  • There are some international standards and organization controling the quality characteristics of varieties and seeds such as UPOV, OECD, ISTA, AOSA. The National List system to check variety performance such as quality characteristics of plant varieties and seeds was established by each countries. The UPOV (International Union for the Protection of New varieties of Plant) regulates the requirement of plant variety protection that is distinctness (D), uniformity (U), stability (S) (here in after called DUS), Novelty and Denomination, and some quality characteristics are used to examine the requirement of plant variety protection by each crops. OECD (Organization for Economic Cooperation Development) seed scheme regulates the seed certification for seed trade between countries. ISTA (International Seed Testing Association) and AOSA(Association for Official Seed Analyst) regulates the seed analysis standard and methods. Most of the countries in Europe has the National List system, which check the value for cultivation and use(VCU), that is to say, variety performance such as yield, quality and stress to environment. All the seeds should be enlisted in the National List before sell the seed in their country. All the quality characteristics checked variety performance are for instance, amylose and amylopectin content in rice, molting quality, $\beta$-glucan, protein in barley, protein and sugar content in soybean, sugar and amylopectin content in corn etc. Conditions for the protection of new variety of plant are DUS. Quality characteristics may be the important characteristics and used to check DUS in crop by crop. It is very important to develop a new characteristics and establish standard method fur examine the VCU and DUS test for each crop.

Effect of Light Intensity on Growth Characteristic and Flower Color Change of New Guinea Impatiens 'Fishlimp 149' (광도 차이에 따라 나타나는 뉴기니아 봉선화 생육 특성 및 화색 변화)

  • Lee, Ho-Sun;Kim, Su-Jeong;Shin, Woo-Gun;Yoo, Byeong-Cheon
    • Journal of Bio-Environment Control
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    • v.15 no.4
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    • pp.406-411
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    • 2006
  • New Guinea impatiens (Impatiens hawkeri) shows very sensitive responses to different light conditions. Due to these phenomena, testers of DUS (distinctness, uniformity and stability) for granting plant variety protection right often have problems distinguishing genetic or physiological differences. New Guinea impatiens 'Fishlimp 149' was grown under several light intensities in a rain-sheltered vinyl house to observe differences of plant growth and flowering. As compared with the control (avg. $1,010{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), treatments of shade-1 (avg. $599{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and shade-2 (avg. $88{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) showed increased plant height, plant width, leaf size and pedicel length. On the contrary, these growth parameters decreased in shade-3 (avg. $30{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) with the lowest light intensity. Shade-1 treatment enhanced flower characteristics such as flower diameter, upper petal width, side petal width and lower petal length. However, these characteristics were suppressed by lower light intensities than that in shade-1. Anthocyanin contents of shoot, leaf and pedicel decreased with increasing shading, but that of flower petal was the greatest of the shade-1 treatment. Shade-1 treatment showed the greatest Hunter a value analyzed by a colorimeter, and L and b values increased with increasing shading. Shade-1 treatment seemed to be provided the most proper light condition for DUS test of New Guinea impatiens. Additionally, anthocyanin accumulation on New Guinea impatiens during DUS test was due to not genetic differences but physiological phenomenon.

Use of SSR Markers to Complement Tests of Distinctiveness, Uniformity, and Stability (DUS) of Pepper (Capsicum annuum L.) Varieties

  • Kwon, Yong-Sham;Lee, Je-Min;Yi, Gi-Bum;Yi, Seung-In;Kim, Kyung-Min;Soh, Eun-Hee;Bae, Kyung-Mi;Park, Eun-Kyung;Song, In-Ho;Kim, Byung-Dong
    • Molecules and Cells
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    • v.19 no.3
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    • pp.428-435
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    • 2005
  • This study was carried out to assess the potential of SSR markers for variety identification by comparing SSR markers and morphological traits in tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Twenty-seven SSR markers were polymorphic in 66 pepper varieties, revealing a total of 89 alleles. Average polymorphism information content (PIC) value was 0.529, ranging from 0.03 to 0.877. Cluster analysis of the band patterns separated the varieties into three groups corresponding to varietal types. Morphological trait-based clustering showed some degree of similarity to dendrogram topologies based on the SSR index. However, no significance correlation was found between the SSR and morphological data. SSR markers could be used to complement a DUS test of a candidate variety and to select complimentary varieties by pre-screening existing varieties in the context of protecting new varieties of pepper.

Cultivation and Characteristic Methods for DUS Test New Varieties of Actinidia arguta -Based on the Process of Writing the Manual- (다래(Actinidia arguta) 신품종 심사를 위한 재배 및 특성조사방법 -매뉴얼 작성과정을 기준으로-)

  • Mi-Yun An;Ro-Young Lee;Je-Min Park;Byeong-Hoon Yang;Ki-Yoon Kim
    • Korean Journal of Environmental Agriculture
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    • v.42 no.4
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    • pp.450-456
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    • 2023
  • The National Forest Seed and Variety Center aims to write a manual for cultivation and characteristic inspection of new seed varieties in Korea, serving dual purposes. The first is the enhancement of the test guidelines, particularly the distinctness, uniformity and stability (DUS) test, for new seed variety, where research and examination are conducted based on the test guidelines. However, if the crop-specific test guidelines are initially reorganized, the details are unclear. Therefore, it seeks to maintain a written record to prevent confusion when the person in charge is changed. The second is to encourage the development of new varieties. By incorporating general characteristics and cultivation techniques in the manual, it seeks to encourage breeders to develop new varieties. Additionally, it serves as an important chronological record of the creation process, thereby helping future manual authors. This article introduces the current status of new plant variety protection and outlines the significance of creating a manual for cultivation and characterization of Actinidia arguta for screening new varieties.

Impact Evaluation of Rubber Type, Hardness and Induced Prestress Force on the Dynamic Properties of a Damper (감쇠장치의 동적특성에 대한 고무의 종류, 경도 및 프리스트레스력의 영향 평가)

  • Im, Chae-Rim;Yang, Keun-Hyeok;Mun, Ju-Hyun;Won, Eun-Bee
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.26 no.6
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    • pp.167-174
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    • 2022
  • The objective of this study is to evaluate the dynamic properties of DUS (damping-up system) composed of the materials with excellent damping capacity, and to compare with those of the conventional hangar bolt. The main parameters are the type and hardness (𝜂H), of rubber and the prestress force (value converted from the compression strain (𝜂R) in the stress-strain relationship of rubber). The dynamic properties were examined from the natural frequency (𝜔n), maximum response acceleration (Am), amplification coefficient (𝛼p), maximum relative displacement (𝚫m), and damping ratio (𝜉D). The test results showed that the Am, 𝛼p, and 𝚫m values of DUS were 46.3%, 46.6% and 62.9% lower, respectively, and the 𝜉D value was 3.89 times higher, when compared to those of the conventional hangar bolt. In particular, the 𝛼p value was 1.3 for DUS, and 2.45 for the conventional hanger bolt, which were similar to those of rigid and flexible components specified in KDS 41 17 00, respectively. Consequently, in the optimal details of DUS, the 𝜂H values of 50 and 45 were required for the NR (natural rubber) and EPDM (ethylene propylene diene monomer), and the 𝜂R value of 5% was also recommended.

Hordein Fingerprinting for Cultivar Discrimination in National List of Barley (Hordein 분석을 통한 보리 국가목록등재품종의 품종식별)

  • 소은희;고은별;최수정;이종호;송인호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.3
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    • pp.256-260
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    • 2004
  • A major challenge facing those involved in the testing of new plant varieties for distinctness, uniformity and stability (DUS) is the need to compare new varieties against all those of common knowlege (reference varieties). One possible approach would be to group new (candidate) varieties and reference varieties using descriptions stored in databases prior to further of official test. testing. This study was carried out to manage a reference variety collection by databasing of hordein profiling. For this purpose, hordein subunits of the 48 National list barley (Hordeum vulgare L) cultivars were analysed. Total 22 of clear scorable hordein subunits were identified from D-subunit to B-subunit region and fifteen different hordein polypeptide patterns were obtained. Based on hordein subunit band pattern, UPGMA cluster analysis was conducted. Forty-eight cultivars were separated into three groups and genetic distance of cluster ranging from 0.55 to 1.00. Hordein subunits have a potential of selecting similar varieties compared with candidate varieties by controlling reference variety collection and playing an important complemental role in cultivar distinctness.

DNA fingerprinting analysis for soybean (Glycine max) varieties in Korea using a core set of microsatellite marker (핵심 Microsatellite 마커를 이용한 한국 콩 품종에 대한 Fingerprinting 분석)

  • Kwon, Yong-Sham
    • Journal of Plant Biotechnology
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    • v.43 no.4
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    • pp.457-465
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    • 2016
  • Microsatellites are one of the most suitable markers for identification of variety, as they have the capability to discriminate between narrow genetic variations. The polymorphism level between 120 microsatellite primer pairs and 148 soybean varieties was investigated through the fluorescence based automatic detection system. A set of 16 primer pairs showed highly reproducible polymorphism in these varieties. A total of 204 alleles were detected using the 16 microsatellite markers. The number of alleles per locus ranged from 6 to 28, with an average of 12.75 alleles per locus. The average polymorphism information content (PIC) was 0.86, ranging from 0.75 to 0.95. The unweighted pair group method using the arithmetic averages (UPGMA) cluster analysis for 148 varieties were divided into five distinctive groups, reflecting the varietal types and pedigree information. All the varieties were perfectly discriminated by marker genotypes. These markers may be useful to complement a morphological assessment of candidate varieties in the DUS (distinctness, uniformity and stability) test, intervening of seed disputes relating to variety authentication, and testing of genetic purity in soybean varieties.

Discrimination of Potato Varieties by Random Amplified Polymorphic DNA Analysis (RAPD에 의한 감자 품종의 구분)

  • Seo, Hyo Won;Yi, Jung Yoon;Cho, Hyun Mook;Park, Young Eun;Oh, Seung Eun
    • Horticultural Science & Technology
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    • v.19 no.1
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    • pp.29-33
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    • 2001
  • This study was carried out to discriminate potato cultivars and breeding lines by specific molecular markers using random amplified polymorphic DNA (RAPD) analysis. The genotypes of potatoes used for analysis were eight cultivars and five breeding lines. Some of those show much phenotypic resemblances among them because 'Jopung', 'Daekwan70', 'Gawon', and 'Daekwan72' have immediate parental relationship with 'Superior', 'Irish Cobbler', 'Namsuh', and 'Atlantic', respectively. So, there are many difficulties to distinguish the varieties by the morphological characteristics. Three URP primers, URP2, URP4, and URP8 were selected for promising primers to discriminate potato genotypes or cultivars. The three URP primers were shown very high reproducibility because of the relatively high annealing temperature and long primer size. Although the results of similarity analyses did not always reflect the genetic relationship between potato varieties, the reproducible pattern of amplified DNA bands by URP primers showed possibility for molecular markers for discrimination of potato genotype or cultivar.

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