• Journal of Ginseng Research
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• 제40권1호
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• pp.62-67
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• 2016

Background: Ginseng, which is widely used in functional foods and as an herbal medicine, has been reported to reduce the proliferation of prostate cancer cells by mechanisms that are not yet fully understood. Methods: This study was designed to investigate the changes in ginsenoside content in ginseng after treatment with a microwave-irradiation thermal process and to verify the anticancer effects of the extracts. To confirm the anticancer effect of microwave-irradiated processed ginseng (MG), it was tested in three human prostate cancer cell lines (DU145, LNCaP, and PC-3 cells). Involvements of apoptosis and autophagy were assessed using Western blotting. Results: After microwave treatment, the content of ginsenosides Rg1, Re, Rb1, Rc, Rb2, and Rd in the extracts decreased, whereas the content of ginsenosides 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5 increased. Antiproliferation results for the human cancer cell lines treated with ginseng extracts indicate that PC-3 cells treated with MG showed the highest activity with an half maximal inhibitory concentration of $48{\mu}g/mL$. We also showed that MG suppresses the growth of human prostate cancer cell xenografts in athymic nude mice as an in vivo model. This growth suppression by MG is associated with the inductions of cell death and autophagy. Conclusion: Therefore, heat processing by microwave irradiation is a useful method to enhance the anticancer effect of ginseng by increasing the content of ginsenosides Rg3, Rg5, and Rk1.

• 한국재료학회지
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• 제11권2호
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• pp.137-145
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• 2001

수용액 매체에서 젤케스팅을 이용하여 복잡한 형상의 세라믹스를 제조하기 위한 새로운 공정을 연구하였다 용융실리카 분말과 첨가제로서 지르콘과 코디어라이트 조성으로 혼합된 현탁액을 electrosteric 방법으로 안정화시켰다. 슬립은 다성분계 세라믹 현탁액에 단량체, 이량체 그러고 분산제를 혼합하고, 볼밀하여 준비하였다. 슬립의 유동학적 특성을 평가하기 위해 점도를 측정하였으며 , 낮은 점도를 갖는 50vo1%의 고농도 다성분계 세라믹 슬립의 제조가 가능하였다. 슬립의 점도는 고분자 분산제의 함량과 단량체 및 이량체의 혼합비에 크게 의존하였다. 성형체는 안정화시킨 슬립을 몰드에 부어 상온에서 젤화시킨 후 $25^{\circ}C$, 80~85% 상대습도 분위기 하에서 48시간동안 건조시켜 제조하였으며 건조된 성형체에는 균열이 발생하지 않았다.

• Molecules and Cells
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• 제45권4호
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• pp.202-215
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• 2022

The androgen receptor (AR) is an important therapeutic target for treating prostate cancer (PCa). Moreover, there is an increasing need for understanding the AR-independent progression of tumor cells such as neuroendocrine prostate cancer (NEPC). Menin, which is encoded by multiple endocrine neoplasia type 1 (MEN1), serves as a direct link between AR and the mixed-lineage leukemia (MLL) complex in PCa development by activating AR target genes through histone H3 lysine 4 methylation. Although menin is a critical component of AR signaling, its tumorigenic role in AR-independent PCa cells remains unknown. Here, we compared the role of menin in AR-positive and AR-negative PCa cells via RNAi-mediated or pharmacological inhibition of menin. We demonstrated that menin was involved in tumor cell growth and metastasis in PCa cells with low or deficient levels of AR. The inhibition of menin significantly diminished the growth of PCa cells and induced apoptosis, regardless of the presence of AR. Additionally, transcriptome analysis showed that the expression of many metastasis-associated genes was perturbed by menin inhibition in AR-negative DU145 cells. Furthermore, wound-healing assay results showed that menin promoted cell migration in AR-independent cellular contexts. Overall, these findings suggest a critical function of menin in tumorigenesis and provide a rationale for drug development against menin toward targeting high-risk metastatic PCa, especially those independent of AR.

• Biomolecules & Therapeutics
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• 제17권4호
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• pp.403-411
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• 2009

Thiacremonone, the main component isolated from heated garlic (Allium sativum L.), is interested for using as a cancer preventive or therapeutic agent since garlic has been known to be useful plant in the treatment of cancers. Nuclear factor kappaB (NF-${\kappa}B$) is constitutively activated in the prostate cancer and activation of NF-${\kappa}B$ is implicated in drug resistance in cancer cells. Docetaxel, a semisynthetic analog of paclitaxel, is an antineoplastic drug widely used for advanced various cancer. In previous studies, we found that thiacremonone inhibited activation of NF-${\kappa}B$ in cancer cells and marcrophages. In the present study, we investigated whether thiacremonone could increase susceptibility of prostate cancer cells (PC-3 and DU145) to docetaxel via inactivation of NF-${\kappa}B$. We found that the combination treatment of thiacremonone (50 ${\mu}g$/ml) with docetaxel (5 nM) was more effective in the inhibition of prostate cancer cell growth and induction of apoptosis accompanied with the significant inhibition of NF-${\kappa}B$ activity than those by the treatment of thiacremonone or docetaxel alone. It was also found that NF-${\kappa}B$ target gene expression of Bax, caspase-3 and caspase-9 was much more significantly enhanced, but the expression of Bcl-2 was also much more significantly inhibited by the combination treatment. These results indicate that thiacremonone inhibits NF-${\kappa}B$, and enhances the susceptibility of prostate cancer cells to docetaxel. Thus, thiacremonone could be useful as an adjuvant anti-cancer agent.

• 한국산림과학회지
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• 제81권2호
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• pp.139-145
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• 1992

본(本) 연구(硏究)는 강원대학교(江原大學校) 임과대학부속(林科大學附屬) 연습림(演習林)의 제(第)2임반(林班)을 대상으로 수치지형도(數値地形圖)를 이용, 임도개설목적(林道開設目的)에 따른 4개의 평가인자(評價因子), 즉 최단임도개설장(最短林道開設長), 평균집재거리(平均集材距離), 개발지수(開發指數) 및 집재불능지점수(集材不能地點數)의 비율(比率) 등을 설정하여 각각의 평가인자(評價因子)에 적합한 최적임도노선(最適林道路線)의 배치계획(配置計劃)에 대하여 검토하였다. 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 최단임도개설장(最短林道開設長)에 의한 노선배치(路線配置)는 총연장(總延長) 6035.6m, 임도밀도(林道密度) 12.73m/ha, 평균집재거리(平均集材距離) 279.9m, 개발지수(開發指數) 1.43, 집재불능지점수(集材不能地點數)의 비율(比率)15.7% 이다. 2. 평균집재거리(平均集材距離)에 의한 노선배치(路線配置)는 총연장(總延長) 7828.501, 임도밀도(林道密度) 16.52m/ha, 평균집재거리(平均集材距離) 198.4m, 개발지수(開發指數) 1.31, 집재불능지점수(集材不能地點數)의 비율(比率) 4.0%이다. 3. 개발지수(開發指數)에 의한 임도노선배치(林道路線配置)는 총연장(總延長) 7410.6m, 임도밀도(林道密度) 15.64m/ha, 평균집재거리(平均集材距離) 210.9m, 개발지수(開發指數) 1.26, 집재불능지점수(集材不能地點數)의 비율(比率) 5.0%이다. 4. 집재불능지점수(集材不能地點數)의 비율(比率)에 의한 임도노선배치(林道路線配置)는 총연장(總延長) 8307.1m, 임도밀도(林道密度) 17.53m/ha, 평균집재거리(平均集材距離) 184.9m, 개발지수(開發指數) 1.29, 집재불능지점수(集材不能地點數)의 비율(比率) 2.5%이다.

• 동의생리병리학회지
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• 제26권5호
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• pp.687-692
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• 2012

Schizandra chinensis extract has been known to possess a variety of efficacy including antitumor. However, it remains unclear how schizandrin, which is a major biological active ingredient of Schizandra chinensis, exerts antitumor effect. This study was designed to investigate the mechanism by which schizandrin inhibits tumor growth and metastasis. In in vivo test using tumor model mice injected with B16BL6 cell line, mice treated with 10 and 100 ${\mu}g/ml$ schizandrin showed a significant inhibition by $73.79{\pm}6.43%$ and $90.46{\pm}1.72%$, respectively, compared with positive tumor controls. Schizandrin did not exert a significant toxicity for the normal cells (HUVECs) and tumor cell lines (A549, B16BL6, Du145, Huh7). Treatment with schizandrin at 10 and 100 ${\mu}g$/head significantly inhibited the tumor-induced angiogenesis by $68.04{\pm}32.21%$ and $103.8{\pm}34.99%$ compared with the positive control group, respectively. Using in vivo lung metastasis model, tumor metastasis assay revealed that 10 and 100 ${\mu}g$/head schizandrin significantly decreased the metastatic lung tumor by $37.51{\pm}8.15%$ and $75.53{\pm}4.38%$ compared with positive controls, respectively. On the other hand, schizandrin did not affect the adherence of B16BL6 cell line to extracellular matrix protein. These results demonstrate that schizandrin exerts inhibitory effect on tumor growth and metastasis by inhibiting angiogenesis. This study thus suggest that schizandrin may be a candidate molecule target for cancer drug development.

• 한국약용작물학회지
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• 제18권6호
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• pp.409-415
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• 2010

In this study, the bioactivities of ethanol (EELS) and water extract (WELS) from the leaf of Lythrum salicaria L. were investigated. In the anti-cancer activity, the growths of both human prostate cancer (DU145) and human colonic carcinoma cell (HT29) were inhibited up 60% by adding 10 mg/$m{\ell}$ of EELS. Anti-inflammatory activity of EELS and WELS have been evaluated on lipopolysaccharide (LPS) induced release of nitric oxide (NO) by the macrophage RAW 264.7 cells. EELS and WELS inhibited inflammatory by 57.3 and 46.9% in 10 mg/$m{\ell}$, respectively. In the anti-oxidative activity, $IC_{50}$ of DPPH radical scavenging activity was respectively 60.71 and $92.90\;{\mu}g/m{\ell}$ by EELS and WELS. In the anti-diabetic activity, $IC_{50}$ of ${\alpha}$-amylase inhibitory activity of EELS and WELS were respectively 5,250 and $5,020\;{\mu}g/m{\ell}$. $IC_{50}$ of ${\alpha}$-glucosidase inhibitory activity was 7.96 and $68.41\;{\mu}g/m{\ell}$ by EELS and WELS. In the anti-obesity, $IC_{50}$ of lipase inhibitory activity was 880 and $9,840\;{\mu}g/m{\ell}$ by EELS and WELS. Finally, EELS and WELS exhibited anti-oxidative, anti-inflammatory, anti-diabetic activity and anti-obesity. It suggests that Lythrum salicaria L. could be potentially used as a resource of bioactive materials for health functional foods.

• 한국식품저장유통학회지
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• 제13권5호
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• pp.649-654
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• 2006

유자의 생리활성 식품 소재로서 검토하기 위해 비타민 C가 첨가된 유자 추출물의 항산화 효과와 암세포 억제활성에 대한 상승효과를 측정하였다. 과육과 과피의 추출물 중에 총 페놀 함량은 각각 건물당 $97.87{\mu}g/mg$와 $121.17{\mu}g/mg$으로서 과피가 과육보다 약 1.24배 높았으며, 총 플라보노이드 함량은 각각 건물당 $0.28{\mu}g/mg$와 $0.59{\mu}g/mg$로서 과피가 과육보다 2.11배 높았다. 전자공여능은 유자 과피의 추출물은 유자 과육의 추출물보다 약간 높았으며, 이들 추출물은 그 자체에 비하여 각각 추출물에 비타민 C를 첨가할 경우가 상승효과는 더 크게 나타났는데, 특히 저농도($30{\mu}g/mL$) 시료보다는 고농도($300{\mu}g/mL$)에서 전자공여능이 크게 증가하였다. 항산화능은 과피 추출물 $1,000{\mu}g$에 비타민 C $500{\mu}g$를 혼합한 경우의 비타민 C $500{\mu}g$ 단독만을 첨가한 경우보다 각각 20분 반응에서 2.6배, 40분 1.5배, 60분에는 1.2배가 높았다. 환원능은 유자 과피 추출물의 $30{\mu}g/mL$와 $50{\mu}g/mL$ 저 농도에서 고농도에서와 비슷하게 아주 높게 나타났다. 유자 과피 및 과육의 추출물로 처리한 간암 세포주에서 다른 암 세포주에 비하여 비교적 항암활성이 높게 나타났으며, $100{\mu}g/mL$에서는 각각 63.8%와 73%를 나타내었다. 유방암 세포주는 모든 시험농도에서 유자 과피의 추출물이 과육 추출물보다 항암활성이 모두 높게 나타났다. 결론적으로 유자 추출물의 항산화 및 항암활성 은 함유되어 있는 페놀과 플라보노이드 성분에 의한 것으로 판단된다.

• 한국식생활문화학회지
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• 제15권2호
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• pp.139-145
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• 2000

A survey on the preference of kimchi and actual amounts of kimchi intake conducted from july to october 1999 to get basic information needed for the augmentation of kimchi intake. Total 1,241 of women and men aged $10{\sim}60$ in kyung-nam area participated in this survey. Except people aged $20{\sim}30$, all of subjects, especially in people aged 60 and over, liked fresh prepared kimchi best, and then fermented kimchi. But all of subjects disliked over fermented kimchi.(p<0.001) And women$(4.3{\pm}0.9)$ liked better fresh prepared kimchi than men$(4.2{\pm}1.0)$, men$(4.0{\pm}0.9)$ liked better fermented kimchi than women$(3.9{\pm}1.1)$.(p<0.05) In subjects aged above 40, as age grow up, the preference of fermented kimchi and over fermented kimchi grew lower. All of subjects best liked chinease cabbage kimchi, and then chonggak kimchi, kakdugi, yeulmu kimchi, dongchimi in order. And women liked better nabag kimchi, yeulmu kimchi, sesame leaf kimchi, bek kimchi, cucumber kimchi, godulppegi kimchi than men and men liked better chinease cabbage kimchi, kakdugi, dongchimi than women.(p<0.05) People aged $10{\sim}20$ and above 60 disliked chonggag kimchi. The preference of kakdugi were high in people aged $10{\sim}20$, but as age grow up, the preference of kakdugi became lower.(p<0.001) The preference of nabak kimchi and dongchimi with juice were significantly high in people aged 60 and over but people aged $10{\sim}20$ disliked. Also subjects aged $10{\sim}20$ disliked significantly yeulmu kimchi, sesame leaf kimchi, green onion kimchi, bek kimchi, cucumber kimchi, mustard leaf kimchi and godulppegi kimchi and subjects aged $20{\sim}30$ disliked significantly yeulmu kimchi, mustard leaf kimchi, godulppegi kimchi(p<0.001). But subject aged 30 and older liked all kinds of kimchi. The amounts of kimchi intake in Kyung-nam area were same level of average amount(100g) of korean intake but the amounts of kimchi intake of men$(106.4{\pm}74.0)$ were more than women$(96.9{\pm}69.5)$(p<0.05)

• 한국토양비료학회지
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• 제27권4호
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• pp.269-274
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• 1994

하우스내에서 메론 재배시(栽培時) 토양수분(土壤水分)이 넷트메론의 수량(收量) 및 품질(品質)에 미치는 영향(影響)을 구명(究明)코자 관수점(灌水點)을 -0.5bar, 관개시기(灌漑時期)를 전생육기(全生育期), 개화시(開花始)-개화후(開花後) 35일, 개화시(開花始)-개화후(開花後) 20일 및 개화시(開花始)-개화후(開花後) 5일 등으로 처리(處理)하여 증발산량, 생육(生育), 수량(收量) 및 품질(品質) 등을 조사(調査), 분석(分析)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 처리별(處理別) 관개량(灌漑量)은 전생육기(全生育期) 동안 관개(灌漑)처리 구에서는 170.5mm, 개화시(開花始)부터 개화후(開花後) 35일까지 관개(灌漑)처리 구에서는 145.0mm, 개화시(開花始)부터 개화후(開花後) 20일까지 관개(灌漑)처리 구에서는 126.9mm, 개화시(開花始)부터 개화후(開花後) 5일까지 관개(灌漑)처리 구에서는 78.8mm였으며 관개(灌漑)횟수는 각각 13, 10, 7, 4회 이었다. 2 메론의 전생육기간(全生育期間)동안 증발산량은 5월 : 106.3mm, 6월 : 109.2mm, 7월 : 62.2mm(상순(上旬)과 중순(中旬))였으며 메론의 일(日) 평균(平均) 증발산량은 3.31mm였고 착과(着果)이후 5일부터 15일까지 증발산량이 가장 많았다. 3. 메론의 수량(收量)은 전생육기간(全生育期間) 관개(灌漑)한 구(區)에서 총수량(總收量)이 3,149kg/10a로 높았으나 상품성(商品性) 수량(收量)은 2,299kg/10a로 낮았고, 개화시(開花始)부터 개화후(開花後) 20일까지 관개(灌漑)한 구(區)에서 상품성(商品性) 수량(收量)이 2,520kg/10a로 가장 양호하였다. 4. 당도 및 넷트는 개화시(開花始)-개화후(開花後) 5일까지 관개(灌漑)>개화시(開花始)-개화후(開花後) 20일까지 관개(灌漑)>개화시(開花始)-개화후(開花後) 35일까지 관개(灌漑)>전생육기(全生育期) 관개(灌漑) 순으로 양호하였다.