• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2014년도 제49차 동계학술대회논문집 22권1호
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• pp.429-432
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• 2014

국내 제약산업의 경쟁력을 제고시키기 위해서는 신약의 심사/허가 기간을 단축시켜 급변하게 변하는 글로벌 제약시장에서 경쟁 우위적 위치를 선점할 수 있도록 기회를 제공할 수 있도록 체계 개선이 시급하다. 신약허가를 위해서는 임상시험 결과에 대한 안전성과 유효성 등에 대한 심사가 수행되게 된다. 하지만 현재 신약허가를 위해서 제약사와 임상시험수탁기관(Contract Research Organization, CRO)에서 데이터 정보체계인 Domain, Variable 및 Parameter 등의 표준을 따르지 않고 다양한 유형의 임상정보데이터를 심사기관에 제출하고 있어 이로 인한 심사기간 증가와 심사업무 비효율성을 야기시키고 있다. 따라서 본 연구에서는 국제민간기구인 CDISC (Clinical Data Interchange Standards Consortium)에서 제정한 글로벌 임상데이터 표준인 CDISC 표준을 준용한 국내 임상시험정보관리 체계 (eCTD 시스템)및 의약품 전주기적 관리체계를 제시하고자 하며, 본 연구를 통한 기대효과로는 국제표준의 임상정보관리 인프라 구축으로 인한 국내 신약개발 및 해외 진출 환경을 마련하여 글로벌 시장선점의 기회를 제공할 수 있고, 규제기관 차원에서는 의약품 허가, 심사업무의 효율성 증가는 물론 전주기적 의약품 안전관리체계를 마련할 수 있을 것으로 사료된다.

• 한국양봉학회지
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• 제33권4호
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• pp.227-237
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• 2018

The majority parasitic bee mites of Thailand in genus Tropilaelaps are infesting colonies of native bees (Apis dorsata) and introduced bees (A. mellifera). The investigation aims to study morphological and genetic variation of Tropilaelaps mites infected different hosts. Adult mites were collected from honey bee brood throughout Thailand. Traditional and geometrical morphometrics were measured on photograph by using TPS program. Additional, COI gene variations were examined by PCR-RFLP and nucleotides sequencing. Tree of mites relationships were constructed by NJ and MP assumptions. Morphometric results indicated T. mercedesae were major species infesting on A. dorsata and A. mellifera. Mophological variation represented at anal and epigynial plate, which the shape of the anal plate apex margin has been key character to identify between T. mercedesae (bell to blunt shape) and T. koenigerum (pear shape). However, the discriminant analysis suggested that geometric results were potential to classify Thai Tropilaelaps populations from different hosts better than traditional morphometric. Otherwise, PCR-RFLP clearly detected the site of Dra I and Xba I digestion of Thai Tropilaelaps morphotypes. The COI sequences of T. koenigerum were founded infesting only A. dorsata in Thailand and four sequences that related to the Thai T. mercedesae morphotypes. The NJ and MP tree were clearly classified Thai Tropilaelaps species which were suggested both from morphological and molecular analysis. This information might be basically of taxonomic status, but this should have implication for controlling these mites in Thailand and other countries.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제16권5호
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• pp.1708-1734
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• 2022

In the development of logistics system, the breakthrough of important technologies such as technology platform for logistics information management and control is the key content of the study. Based on Javascript and JQuery, the logistics system realizes real-time monitoring, collection of historical status data, statistical analysis and display, intelligent recommendation and other functions. In order to strengthen the cooperation of warehouse storage, enhance the utilization rate of resources, and achieve the purpose of real-time and visual supervision of transportation equipment and cargo tracking, this paper studies the VNF dynamic deployment and SFC routing problem in the network load change scenario based on the logistics system. The BIP model is used to model the VNF dynamic deployment and routing problem. The optimization objective is to minimize the total cost overhead generated by each SFCR. Furthermore, the application of the SFC mapping algorithm in the routing topology solving problem is proposed. Based on the concept of relative cost and the idea of topology transformation, the SFC-map algorithm can efficiently complete the dynamic deployment of VNF and the routing calculation of SFC by using multi-layer graph. In the simulation platform based on the logistics system, the proposed algorithm is compared with VNF-DRA algorithm and Provision Traffic algorithm in the network receiving rate, throughput, path end-to-end delay, deployment number, running time and utilization rate. According to the test results, it is verified that the test results of the optimization algorithm in this paper are obviously improved compared with the comparison method, and it has higher practical application and promotion value.

• Clinical and Experimental Pediatrics
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• 제55권11호
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• pp.420-423
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• 2012

Purpose: We phylogenetically analyzed the Escherichia coli strains isolated from children with urinary tract infection (UTI) in 2 regions of Korea. Virulence factors (VFs) and antibiotic resistance of the strains were also determined to compare the possible differences. Methods: A total of 138 E. coli strains were collected from the 2 regions; Gyeongin (78 strains) and Gyeongnam (60 strains). The phylogenetic groups were determined using the triplex polymerase chain reaction (PCR) method and multiplex PCRs were used to detect 7 VFs genes (fimH, papC, iutA, hlyA, sfa/focDE, afa/draBC, and kpsMT II). We also tested for antibiotic resistance. Results: Phylogenetic groups, B2 (61.6%) and D (26.8%), comprised the majority of all isolated strains. Regional comparisons revealed that more B2 strains and fewer non-B2 (A+B1+D) strains were found in Gyeongnam, than in the Gyeongin region (P=0.033), and certain VFs were predominantly detected in Gyeongnam (P<0.05). Neither regional nor phylogenetic differences, in antibiotic resistance of the strains, were significant. Conclusion: We were able to confirm that the geographic location is an important determinant of the distribution of the phylogenetic groups and VFs among the E. coli strains that cause UTI in children.

• Asian-Australasian Journal of Animal Sciences
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• 제17권6호
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• pp.743-749
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• 2004

The gene encoding human serum albumin (HSA) was cloned from human liver cDNA library by PCR. The HSA cDNA in size of 2,176 bp, including 1,830 bp of open reading frame, was cloned into the plasmid carried with the 5'flanking sequence of goat $\beta$-casein gene (-4,044 to +2,025 bp) to get a tissue specific expression vector in mammary gland named pGB562/HSA (12.5 kb). A 9.6 kb DNA fragment in which the sequence is in order of goat $\beta$-casein gene regulatory sequence, HSA cDNA and SV40 polyadenylation signals was isolated from the pGB562/HSA by SacI and DraIII cutting, and used to microinject into the pronuclei of mouse fertilized eggs to produce transgenic mice. Three transgenic mice (2 female and 1 male) were identified by PCR and dot Southern blot analysis. The copy numbers of integrated transgene were more than 10 copies in line #21 and #26 as well as over 50 copies in line #31 of transgenic mice. HSA protein collected from the milk of lactating transgenic mice was confirmed by immuno-detection of Western and slot blot. The concentrations of HSA in the milk were from 0.05 to 0.4 mg/ml. An obvious antigen and antibody conjugate could be observed in immunohistochemical stain of mammary gland tissue from lactating day 11 of HSA transgenic mice. The transmission of transgene and its expression was recognized according to the results of RT-PCR and sequences analyses of their progeny.

• 한국조경학회지
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• 제32권3호
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• pp.18-31
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• 2004

Ecological network can solve the ecological problems such as habitat cutting and fragmentation that are resulted from urbanization. Recently, rooftop biotope construction has been appeared as a usefull method for ecological networking in urban area. The objective of this study is to seek the possibility of rooftop greening introduction as well as to select target species for rooftop greening construction from the urban ecological network point of view. In order to select target species, we monitored two adjacent rooftop greening sites, where we found 22 species of birds and insects and 33 species of plants. We set criteria to select target species through documents research, and gave marks species according to the criteria with the help of specialists. We divided the target species into 5 groups on the basis of the 12 selecting criteria. In those two monitored rooftop greening sites, we did not find any species belong to group A, which is the best target species, but we found species belong to the other groups. Orthetrum albistylum, Sympetrum dawinianum belong to group B, which is the priority target species, 7 species besides Passer montanus, Coccinella axyridis, Agrionidae to group C, which is the possible target species, 6 species besides Atractomorpha lata to group D, which is the potential target species, and the others were found to be inadequate to the target species. We found Orthetrum albistylum, Sympetrum dawi-nianum to be the best appropriate target species in the two sites. According to the result of this study, the method of ecological network construction from the dra-gonfly habitat network point of view through the construction of rooftop habitat is ideal for Orthetrum albistylum, and Sympetrum dawinianum. For successful urban ecological network construction, selection criteria and construction techniqes & methods for rooftop habitat should be developed through future research.

• 천문학회보
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• 제41권2호
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• pp.57.3-58
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• 2016

A symbiotic star is a wide binary system consisting of a hot white dwarf and a mass losing giant, where the giant loses its material in the form of a slow stellar wind resulting in accretion onto the white dwarf through gravitational capture. Symbiotic stars are known to exhibit unique spectral features at 6825 and 7082, which are formed from O VI 1032 and 1038 through Raman scattering with atomic hydrogen. In this Monte Carlo study we investigate the flux ratio of 6825 and 7082 in a neutral region with a geometric shape of a slab, cylinder and sphere. By varying the amount of neutral hydrogen parametrized by the column density along a specified direction, we compute and compare the flux ratio of Raman scattered O VI 6825 and 7082. In the column density around 1020 cm-2, flux ratio changes in a complicated way, rapidly decreasing from the optically thin limit to unity the optically thick limit as the column density increases. It is also notable that when the neutral region is of a slab shape with the O VI source outside the slab, the optically thick limit is less than unity, implying a significant fraction of O VI photons escape through Rayleigh scattering near the boundary. We compare our high resolution CFHT data of HM Sge and AG Dra with the data simulated with finite cylinder models confirming that 'S' type symbiotic tend to be characterized by thicker HI region that 'D' type counterparts. It is expected that this study will be useful in interpretation of the clear disparity of Raman O VI 6825 and 7082 profiles, which will shed much light on the kinematics and the asymmetric distribution of O VI material around the hot white dwarf.

• 한국전자파학회논문지
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• 제26권5호
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• pp.445-454
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• 2015

본 논문에서는 고이득 이중 반사경 안테나(DTA: Dual Reflector Antenna)의 성능 향상을 위한 최적설계 기법으로 유전 알고리즘(GA: Genetic Algorithm)을 적용하였다. 또한, 최적설계과정에서 반복해석에 요구되는 계산 시간을 줄이고자 ADE 안테나의 각 반사경의 표면전류분포 계산에 반복적 물리광학법(IPO: Iterative Physical Optics)을 이용하였다. 물리광학법 적용시 음영지역에 대한 고려 및 다중반사에 의한 영향을 MFIE(Magnetic Field Integral Equation) 기반의 반복적인 계산을 통해서 해의 정확도를 향상시켰다. 또한, 설계변수의 축소 및 제작 가능한 부드러운 곡면 형성을 위하여 베지어 곡선을 적용하였다. 이럴 경우, 베지어 곡선의 제어점이 설계변수로 설정이 된다. 최적설계를 위한 목적함수로 HPBW(Half Power Beam Width), FNBW(First Null Beam Width), SLL(Side Lobe Level) 등을 고려하였으며, 설계 및 해석의 결과를 기존의 상용 해석프로그램과 비교하였다.

• 한국주조공학회지
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• 제21권1호
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• pp.7-14
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• 2001

The research on new DRA(discontinuous reinforced alloy) and CRA(continous reinforced alloy) composites has been carried out to improve the properties of ceramic fiber and particle reinforced metal matrix composites(MMCs). Effects of alloying elements and aging conditions on the microstructures and aging behavior of Al-Si-Cu-Mg-(Ni)-SiCp composite have been examined. The specimens used in this study were manufactured by duplex process. The first squeeze casting is the process to make precomposite and the second squeeze casting is the process to make final composite. The hardening behavior was accelerated with decreasing the size of SiCp particle in the composites. It is considered that the dislocation density increased with increasing SiCp size, due to the different thermal deformation between Al matrix and SiCp during quenching after the solution treatment. Peak aging time to obtain the maximum hardness in 3 ${\mu}m$ SiCp reinforced Al composite was reduced than that in large size(5, 10 ${\mu}m$) of SiCp because of difference in dislocation density. Aging hardening responce(${\Delta}H$ = $H_{Max}.-H_{S.T}$) of composites was greater than that of unreinforced Al alloy because of higher density of second phases in matrix.

• BMB Reports
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• 제43권3호
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• pp.164-169
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• 2010

CpG-DNA, which contains unmethylated CpG dinucleotides in the context of specific sequences, has remarkable and diverse immunological effects, including induction of proinflammatory cytokine expression and regulation of the Th1/Th2 immune response. Here, we examined the immunostimulatory activities of double-stranded (ds) CpG-DNA in the human B cell line RPMI8226. To investigate whether dsCpG-DNA stimulates immune cells, we constructed a plasmid containing repeated dsCpG-DNA and produced dsCpG-DNA by PCR amplification and EcoR I digestion. PCR-amplified dsCpG-DNA alone did not have immmunostimulatory activity. However, dsCpGDNA encapsulated with lipofectin induced IL-8 promoter activation, HLA-DRA expression, and IL-8 expression in a CG sequence-independent manner. The effects of encapsulated dsCpGDNA were independent of minor endotoxin contamination. These findings suggest the potential use of dsCpG-DNA as a therapy for immune response regulation.