• 대한의용생체공학회:의공학회지
• /
• 제21권3호
• /
• pp.255-260
• /
• 2000

본 연구는 중환자실, 응급실, 수술실등 병실내에서 환자를 구속하지 않고. 무선으로 환자의 활력징후 신호등을 관찰할 수 있는 생체신호 무선 전송장치를 설계 제작하는데 목적을 두었다. 본 무선 환자감시장치는 활력징후 신호 수집기, 무선 송수신 장치 및 활력징후 관찰기로 구성된다. 활력징후 신호 수집부는 생체신호를 증폭하기 위한 아날로그신호 증폭기와 디지털데이터 변환을 위한 단일 칩 마이크로 컨트롤러로 구성된다. 전송신호의 품질을 확보하고 신호처리 및 구성이 간단하여 저 비용으로 구성할 수 있는 주파수편이변조(FSK) 방법을 사용하였고 디지털신호는 UHF 대역의 미약 무선주파수에 의하여 송수신되었다. 활력징후 신호 관찰기는 무선 수신기에 의해 디지털 데이터를 복조하고 활력징후 신호를 상시 모니터링하기 위한 액정모니터(LCD) 및 신호를 기록하기 위한 감열기록장치(thermal Printer)로 설계 제작되었다.

• 한국발생생물학회지:발생과생식
• /
• 제14권3호
• /
• pp.155-162
• /
• 2010

The trophectoderm is one of the earliest cell types to differentiate in the forming placenta. It is an important for the initial implantation and placentation during pregnancy. Trophoblast stem cells (TBSCs) develop from the blastocyst and are maintained by signals emanating from the inner cell mass. However, several limitations including rarity and difficulty in isolation of trophoblast stem cells derived from blastocyst still exist. To establish a model for trophoblast differentiation, we isolated TBSCs from human term placenta ($\geq$38 weeks) and characterized. Cell cycle was analyzed by measuring DNA content by FACS analysis and phenotype of TBSCs was characterized by RT-PCR and FACS analysis. TBSCs have expressed various markers such as self-renewal markers (Nanog, Sox2), three germ layer markers (hNF68, alpha-cardiac actin, hAFP), trophoblast specific markers (CDX-2, CK7, HLA-G), and TERT gene. In FACS analysis, TBSCs isolated from term placenta showed that the majority of cells expressed CD13, CD44, CD90, CD95, CD105, HLA-ABC, cytokeratin 7, and HLA-G. Testing for CD31, CD34, CD45, CD71, vimentin and HLA-DR were negative. TBSCs were shown to decrease the growth rate when cultured in conditioned medium without FGF4/heparin as well as the morphology was changed to a characteristic giant cell with a large cytoplasm and nucleus. In invasion assay, TBSCs isolated from term placenta showed invasion activities in in vivo using nude mice and in vitro Matrigel system. Taken together, these results support that an isolation potential of TBSCs from term placenta as well as a good source for understanding of the infertility mechanism.

• 한국발생생물학회지:발생과생식
• /
• 제22권1호
• /
• pp.39-53
• /
• 2018

The purpose of this study is to investigate the early life history of the Rhodeus fish, Rhodeus uyekii and R. ocellatus, in the Nakdong River to use as the preliminary data for the systematic study. The embryos used in the study were fertilized eggs (embryo) and larvae after artificial fertilization. The long diameter of the eggs of the R. uyekii was 3.39-3.97 mm (average $3.68{\pm}0.41mm$, n=30) and the short diameter was 1.36-1.55 mm (average $1.45{\pm}0.13mm$, n=30). The long diameter of the eggs of the R. ocellatus was 2.53-2.71 mm (average $2.62{\pm}0.12mm$, n=30) and the short diameter was 1.47-1.60 mm (average $1.53{\pm}0.09mm$, n=30). Hatching time was 48 hours for the R. uyekii and 50 hours for the R. ocellatus given that the average water temperature was $21.5^{\circ}C$. The hatched larvae were 4.95-5.00 mm (average $4.98{\pm}0.04mm$, n=5) for the R. uyekii and the total length was 3.66-3.69 mm (average $3.67{\pm}0.02mm$, n=5) for the R. ocellatus. R. uyekii was found to be 15.5-15.8 mm at total length (average $15.6{\pm}0.21mm$, n=5) on the 56 days after hatching with the number of dorsal fins being ⅲ-9, anal fins ⅲ-10, ventral fins ⅲ-5. The R. ocellatus was found to be 15.8-16.0 mm (average $15.9{\pm}0.14mm$, n=5) at total length on the 58 days with the number of dorsal fins being ⅲ-11, anal fins ⅲ-12 and ventral fins ⅲ-5 where the number of all fin stalks reached maximum.

• 한국발생생물학회지:발생과생식
• /
• 제24권3호
• /
• pp.187-196
• /
• 2020

The caspase10 encodes an initiating caspase that plays an important role in the maintaining the cellular homeostasis by regulating the steps involved in the immune response and cell death. We investigated the expression of caspase10 during the different developmental stages and in olive flounder tissues. Caspase10 increased in the late stage of the formation of immune tissue, and high expression was observed in the gills, kidney, skin, and spleen. The current study analyzed the expressional changes of caspase10 in olive flounder infected with viral hemorrhagic septicemia virus (VHSV). One of the major causes of mass mortality, VHSV infection in olive flounder attributes to significant expression of caspase10 in the gills, spleen, skin, and kidneys. The results indicate a close association of caspase10 expression with the immune response to VHSV infection in olive flounder. The observations could form the basis data for exploration of other fish immune system.

• 한국발생생물학회지:발생과생식
• /
• 제24권3호
• /
• pp.159-165
• /
• 2020

Previously, we reported adverse effects of low-dose nonylphenol (NP) exposure on the reproductive parameters of F1 female mice. In the present study we further investigated the pathohistological effect of NP exposure on the reproductive organs in F1 female mice. NP exposures were continuously conducted from parental pre-mating period until the postnatal day (PND) 33 of F1 offspring for vaginal examination. Mice were sacrificed on PND 30 and the reproductive tissue weights were measured. The initial (at PND 21) body weights of the NP-50 group animals were significantly lower than those of control group animals, and the weight deficit were recovered when the terminal (PND 33) body weights were measured. Early vaginal opening was found in NP group animals (p<0.05). Pathohistological studies revealed that NP-treated F1 animals showed prominent increase in the ovarian follicle numbers (p<0.01), and decrease in the diameter of uterine myometrium (p<0.01), and increase in the diameter of luminal epithelium (p<0.05). The present study demonstrated that the subchronic low-dose NP exposure induced early beginning of puberty and pathohistological abnormalities in ovary and uterus of F1 mice. Further studies are needed to achieve a better understanding on the action mechanism of NP in pubertal onset and to find a way to avoid a hazardous situation provoked by NP exposure.

• 한국환경교육학회지:환경교육
• /
• 제21권2호
• /
• pp.80-88
• /
• 2008

As the gravity of the issue of the global warming surfaced, environmental protection and energy conservation have become one of the most serious issues that global society faces. At the status quo, there are criticisms for not effectively educating the students although the problem is very severe. In middle school, for instance, only 10% of the school offer environmental education while only $20{\sim}30%$ of the high school offer it. As witnessed by these examples, we can infer that there are not well-structured, effective environmental education for teenagers. Recently, NIE(Newspaper-In-Education) has been used as effective means for some fields of middle and/ or high school education. If so, it also could be used for environmental education. The researches, however, on the topic of NIE on environmental education are very rare. In this study, the effect of NIE on environmental education was empirically tested. Our basic assumption is that NIE will cause differences in environmental perception; the cognition and concern. And environment education through NIE will cause the high level of cognition, concern, and practice regarding environment issue. Using simple experimental design and regression analysis, we tested the effect of NIE on cognition, and, concern of environment issue, and then, on the practice of environment-improving action. The "cognition level" did not show significant difference between the experimental group(which was exposed to NIE) and control group(which was not exposed to NIE). The "concern level" for environment showed significant difference. Finally, the "practice level" showed highly significant difference between the experimental and the control group. According to regression analysis, "concern" and "NIE"(dummy variable) was turned out to be statistically significant variables for practice of environment improving action. Therefore, we came up with the conclusion that NIE, which provides the most up-to-date information regarding the environmental status, can serve as the good supplementary mechanism for the effective environment education in the present Korean middle school curriculum system. The statistical result also suggests that each individual school needs to employ and extend NIE method in its environment education curriculum.

• 의학교육논단
• /
• 제9권2호
• /
• pp.57-66
• /
• 2007

Purpose How can be a great doctor with excellence and ethics? In this study, I wanted to find out the characteristics of human environment to make a great doctor. Methods: First, I researched factors and construct of the human environment. So I conceived a model for analyzing human environment with two construction model : Howard Gardner's System Model and Bron-fenbrenner's ecological systems model. Second, I analyzed the life of the Oliver R. Evison M.D. and Ki Ryu Jang M.D. Oliver R. Evison was the pioneer of medicine of Korea and establisher of the Severance Hospital and medical college. Dr KiRyu Jang, who was called 'Schweitzer of Korea', was a good doctor of the poor and weak patients in Korea. Third, I tried to find out a new human environment model to make a great doctor. Results One model for analyzing human environment was made of relationship based on emotion. relationship teaching knowledge and skill, and relationship communicating on value. In the light of analyzing of two great doctors. Oliver R. Evison M.D. and KiRyu Jang M.D, I found out special interrelationship, Hardie, Allen, Severance for Evison, Kyosin Kim, Kyucheol Choi etc. for Ki Ryu Jang These special people were religious actors or social thinkers. Conclusions: To be a great doctor to excel and innovate medical field, medical students should have the chance to meet with people based on religious, ethical and social action, discuss on value across social fields, and can construct the idea to make and realize higher value of medical action. In sum, another important human environment for medical students would be a person who could be communicate with true value.

• 한국수자원학회:학술대회논문집
• /
• 한국수자원학회 2017년도 학술발표회
• /
• pp.128-128
• /
• 2017

준설(Dredging)은 수중에서의 토사굴착이며, 하천유로의 확장, 항만의 수심증가, 매립이나 축제용의 토사채취 등의 목적으로 행해진다. 하천의 준설은 하천의 흐름 특성 및 제반 여건 변화를 가져오기도 한다. 준설 직후 낮아진 하상으로 수위가 낮아지는 경우가 있는 반면, 수위 저하를 동반하지 않는 경우 준설 부근에서 퇴적이 발생하며 이는 하천의 수위 상승으로 이어 질 수 있다. 또한 퇴적으로 인하여, 최심 하상고가 높아지면 상승정도에 따라 준설 시기를 결정해야 한다. 따라서 본 연구는 SCHISM(Semi-implicit Cross-scale Hydroscience Integrated System Model)모형을 이용하여 이상화 수로에서 준설로 인한 하상변동 후 기존 단면으로 복구되기까지 소요되는 시간과 그에 따른 하천의 수리특성 영향을 검토하였다. SCHISM 수치모형은 Virginia Institute of Marine Science의 Dr. Zhang 교수가 개발한 3차원 수치모의 프로그램으로 현재 중국에서 황허강(Yellow River)의 하상변동 관련 연구를 수행하는데 많이 사용되고 있다. 하천 지형은 이상화 수로로 하천설계기준(2009)을 참고하여 제방 경사는 1:3이며, 수로 제원은 4대강 살리기 사업을 통하여 준설이 실시된 국가하천 자료를 참고하였다. 격자간격은 10 m인 사각격자 이며 모의 시간은 하상변동을 일으키는 유량 개념인 유효유량을 적용하여 60일로 설정하였다. 수치모의를 통하여, 최심 하상고 변화 및 하상변동량을 확인하여 침식 및 퇴적 구간을 구분하였고, 시간에 따른 기준 단면으로의 복구 정도를 유량과 유사량을 변경하면서 민감도 분석을 수행하였다. 향후 본 연구는 하천 준설 계획 시 참고자료로 활용이 가능할 것이며, 준설로 인한 하천수리특성 변화 및 준설시기 결정의 선행연구로써 의미가 있다고 판단된다.

• 한국발생생물학회지:발생과생식
• /
• 제23권1호
• /
• pp.35-42
• /
• 2019

Fish shows great difference in growth rate between individuals during larval development and early growth. This difference seriously reduces the production efficiency in fish culture. Growth hormone (GH)/Insulin-like growth factor 1 (IGF1) system is said to play some pivotal roles in fish growth. In this study, we investigated differences of GH, IGF1 and GHR gene expressions in juvenile red spotted grouper (Epinephelus akaara) with different growth performance. Red spotted groupers were reared under the same environmental condition (water temperature $24{\pm}1^{\circ}C$, natural light) for 96 days after hatching. They were divided into 3 groups by size (fast growing, middle growing and slow growing groups: FGG, MGG, and SGG, respectively). RNA was extracted from the brain, liver and muscle tissues from each group, and target gene expression was examined by real-time PCR. In the brain with pituitary gland, expression of GH gene in FGG was significantly higher than the expression in SGG, but the expression of IGF1 and GHR genes in the muscle was highest in SGG. Difference of GHR and IGF1 mRNA in the liver between groups with different growth performance was less clear than that in other tissues, although level of IGF1 mRNA was higher in SGG than in MGG. These results suggest that hormonal governing of growth is not the same in fast growing and slow growing fish, and size grading could cause a shift of hormonal state and growth pattern in this species.

• 한국발생생물학회지:발생과생식
• /
• 제23권2호
• /
• pp.139-148
• /
• 2019

Rising of water temperature due to global warming is a great concern to aquaculturists and fishery biologists. Hence, the present study aimed to investigate the effects of high water temperature on juvenile red spotted grouper, Epinephelus akaara based on the evaluation of stress responses in blood. E. akaara juveniles were exposed to different thermal conditions ($25^{\circ}C$, $28^{\circ}C$, $31^{\circ}C$, and $34^{\circ}C$) for 6 weeks following 2 weeks of acclimation at $25^{\circ}C$. Blood cell morphology and number were examined at three sampling points (2, 7, and 42 days) from a total of 180 fish. Major erythrocytic cellular abnormalities (ECA) observed in blood smears of thermally stressed groups ($31^{\circ}C$ and $34^{\circ}C$) after 6 weeks were echinocytes, teardrop-like cells, swollen cells and vacuolated cells. Both red and white blood cell number (RBC and WBC) were significantly (p<0.05) elevated in $31^{\circ}C$ and $34^{\circ}C$ group after 6 weeks thermal exposure. Differential leucocytes number showed significant increases in neutrophil (N) and decreases in lymphocytes (L) in the highest temperature ($34^{\circ}C$). Different N:L ratio was observed at different thermal conditions which can be used as a reliable alternative to measure stress response. Taken together, these results suggest that higher temperature ($31^{\circ}C$ and $34^{\circ}C$) can interfere the immune system of red spotted grouper by altering the blood cell morphology and number.