• 제목/요약/키워드: DQ

검색결과 325건 처리시간 0.023초

HeLa.S3(SC) 세포계의 집락형성에 미치는 방사선의 효과 (Radition Effect on Colony Formation of HeLa.S3(SC) Cell Line)

  • 신세원;김성규;김명세
    • Journal of Yeungnam Medical Science
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    • 제10권1호
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    • pp.212-217
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    • 1993
  • 임상적으로 많이 이용되는 범위의 방사선 조사에서는 증식능의 상실을 세포사망으로 정의를 내린다. 본 연구는 실험용 orthovoltage조사기를 이용하여 확립된 세포주인 Hela.53(5C)를 대상으로 각각 100 cGy, 200 cGy, 400 cGy 및 600 cGy 방사선조사 후 2주일간 배양후 crystal violet 염색을 하여 육안적으로 판별가능한 크기의 집락의 수를 산출하여 다음의 결과를 얻었다. HeLa.S3(SC) 세포의 집락 형성능은 0.464이었다. 세포의 생존곡선의 형태는 다표적, 1 hit 형태를 보였다. Do는 150 cGy, Dq는 80 cGy, 외삽치는 1.7이었다. 이 연구결과는 향후 다양한 방사선 생물학 연구에 참고자료로 이용 가능하리라 사료된다.

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Cloning and Characterization of Squalene Synthase (SQS) Gene from Ganoderma lucidum

  • Zhao, Ming-Wen;Liang, Wan-Qi;Zhang, Da-Bing;Wang, Nan;Wang, Chen-Guang;Pan, Ying-Jie
    • Journal of Microbiology and Biotechnology
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    • 제17권7호
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    • pp.1106-1112
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    • 2007
  • This report provides the complete nucleotide sequences of the full-length cDNA encoding squalene synthase (SQS) and its genomic DNA sequence from a triterpene-producing fungus, Ganoderma lucidum. The cDNA of the squalene synthase (SQS) (GenBank Accession Number: DQ494674) was found to contain an open reading frame (ORF) of 1,404 bp encoding a 468-amino-acid polypeptide, whereas the SQS genomic DNA sequence (GenBank Accession Number: DQ494675) consisted of 1,984 bp and contained four exons and three introns. Only one gene copy was present in the G. lucidum genome. The deduced amino acid sequence of Ganoderma lucidum squalene synthase (GI-SQS) exhibited a high homology with other fungal squalene synthase genes and contained six conserved domains. A phylogenetic analysis revealed that G. lucidum SQS belonged to the fungi SQS group, and was more closely related to the SQS of U. maydis than to those of other fungi. A gene expression analysis showed that the expression level was relatively low in mycelia incubated for 12 days, increased after 14 to 20 days of incubation, and reached a relatively high level in the mushroom primordia. Functional complementation of GI-SQS in a SQS-deficient strain of Saccharomyces cerevisiae confirmed that the cloned cDNA encoded a squalene synthase.

사상체질(四象體質)과 HLA type과의 상관성(相關性)에 관(關)한 연구(硏究) (A study on the correlation between Sasang Constitution and HLA type)

  • 고병희;송일병;하만수
    • 사상체질의학회지
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    • 제14권1호
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    • pp.90-99
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    • 2002
  • Exact constitutional classification is especially important in Sasang Constitutional Medicine. Therefore, most studies on the Sasang Constitutional medicine have been focused on how accurately to classify Sasang Constitution. Recently, there have been reported on the studies about relationship between genetic polymorphism and Sansang Constitution. The purpose of this study is to investigate whether there is any correlation between Sasang Constitution and HLA type. Polymorphism of HLA genes have been known to be important role in transplantation, autoimmune disease, immune response against infection. This study was concentrated on a relationship of HLA-A, -C, -DR, and -DQ types with Sasang Constitution. Results are as following. 1. In the case of HLA-A, there was no significant difference. Only $HLA-A^*31$ has a tendency which Soyangin is less frequent than Soeumin and Taeumin. 2. In the case of HLA-C, there was significant difference between Sasang Constitution and HLA type. In persons having $HLA-C^*04$ allele, Taeumin is more frequent than Soyangin and Soeumin. On the other hand, Soyangin is more frequent than Taeumin and Soeumin in $HLA-C^*07$ allele. $HLA-C^*14$ has a tendency which Soeumin is more frequent than Soyangin and Taeumin. 3. In. the case of HLA-DR type and HLA-DQ type, there was no significant difference among Sansang Constitution. On the study of correlation between Sasang Constitution and HLA type, there was little statistical significance and tendency. This study was the first try about presentation of frequency on the HLA alleles corresponding with Sasang Constitution. I think that more study on the relationship between HLA gene and Sasang Constitution is necessary by method of large case research and various statistical analysis.

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Y 결선된 7상 BLDC 전동기의 구형파 전류 제어를 위한 새로운 전류 제어방식에 관한 연구 (A Study on New Current Control Method for Square Current Wave in Y Connected 7-Phase BLDC Motor Drive System)

  • 문종주;이원;김장목
    • 전기학회논문지
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    • 제65권4호
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    • pp.576-585
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    • 2016
  • The current control methods of Y-connected 7 Phase BLDC motor are sine wave current control and square wave control. The sine wave current control method needs dq axis transformation of $7{\times}7$ matrix for current control and very complex. Also this method is not suitable for multi Phase BLDC motor of trapezoidal back emf wave. Therefore, in Y connected multi phase BLDC motor, the square wave current control methods are required. Generally, in the 3Phase BLDC system, Average current control method is used for current control. The average current is obtained that the summation of absolute value of each phase current magnitude is divided by the number of conduction phase. However, if average current control method is applied to multi-phase system, there is a problem that each phase currents are different. This problem affects unbalance of each phase torque and fluctuation of total torque. This paper proposed each phase current control method of Y connected 7Phase BLDC system. Proposed method is used for PI controller of each phase for each phase current control. This method can perfect square wave current control. Also, configuration of the method is easier than DQ axis transformation. Proposed method is verified through simulation and experiments.

육군 항공기류 UHF 대역 송수신기 CE101 시험 (The CE101 Test of the Army Aircraft's UHF Band Transceiver)

  • 서정원;정병구;윤창배;신영준
    • 한국전자파학회논문지
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    • 제26권11호
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    • pp.992-998
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    • 2015
  • 육군 항공기류의 UHF 대역 송수신기는 규격인 MIL-STD-461F Figure CE101-4를 만족해야 한다. 항공기에 장착되는 장비이므로, 소모전력 최소화 및 주파수 간섭 최소화에 중점을 두고 장비를 설계하였다. 장비 설계 후 CE101 시험을 진행하면서 문제가 발생되었던 부분은 30 Hz에서 1 kHz에서 100 dBuA 규격 이상의 신호가 발생하였고, 발생된 신호는 송수신기에서 방사되는 10 W, 20 ms 단위의 $T_{DD}$ 50 Hz 신호로 분석되었다. 규격 만족을 위해 전류변화량을 줄여야 한다는 시험 결과를 토대로 출력증폭기의 $I_{dq}$$I_d$ 값을 유사하게 수정하였고, 기존 설계의 소모 전력과 주파수 간섭과 크게 차이가 없게 설계 및 제작하였다.

패턴인식 기법을 이용한 유도전동기 구동용 전압형 인버터의 고장진단 (Fault Diagnosis of Voltage-Fed Inverters Using Pattern Recognition Techniques for Induction Motor Drive)

  • 박장환;박성무;이대종;김동화;전명근
    • 조명전기설비학회논문지
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    • 제19권3호
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    • pp.75-84
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    • 2005
  • 유도전동기 구동시스템의 예상치 않은 고장은 많은 산업 응용분야에서 심각한 문제를 초래시킬 수 있으므로, 유도전동기 구동을 위한 전압형 PWM 인버터의 고장진단에 대해 연구한다. 진단의 고려 대상은 정류기 다이오드, 스위칭 장치 및 입력단의 개방회로 고장이며, 진단신호는 전동기 전류로부터 유도한다. 고장의 특징추출은 dq-전류 경로의 크기를 이용하였고, 진단은 PCA와 LDA를 적용한다. 또한, 본 논문에서는 일반적인 중${\cdot}$소형 유도전동기 구동 시스템의 제어기에 진단 소프트웨어를 추가하여 사용하는 것에 대한 가능성을 제시하며, 그에 관련해 수행속도에 따른 진단결과들을 보여준다. 최종적으로, MATLAB을 이용하여 인버터의 고장진단에 대한 모의실험을 수행 하였고, 제안된 알고리즘의 유용성을 검증하였다.

고장진단을 위한 영구자식 동기전동기의 권선 단락에 의한 고장모델 연구 및 특성해석 (A Study on Stator Winding Turn-Fault Model for Fault Diagnosis in Inverter-Driven Permanent Magnet Moor Drives)

  • 김경화;최동욱;구본관;정인성
    • 조명전기설비학회논문지
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    • 제23권5호
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    • pp.18-28
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    • 2009
  • 고정자 권선의 단락으로 인한 고장을 해석하고 진단 알고리즘의 효과적인 시험 평가를 위해 사용될 수 있는 인버터 구동 영구자석 동기전동기의 고장모델이 제시된다. 기존에 전동기의 해석과 제어에 많이 사용되는 dq 모델은 상전압 모델을 변환한 것으로 전동기 고정자의 권선 단락 시에는 더 이상 3상평형 조건이 성립하지 않기 때문에 인버터 극전압으로부터 전동기 입력 전압을 구하기가 쉽지 않아 고장모델의 해석을 위해서 사용하기 어렵다. 이를 해결하기 위해 전동기 3상 변수와 선전압 관계식을 이용한 전동기의 고장모델이 제안된다. 제안된 고장모델의 타당성을 입증하기 위해 시뮬레이션이 수행되며 내부 고정자의 권선 단락이 가능하도록 제작된 전동기와 DSP TMS320F28335를 이용한 제어 시스템에 의해 동일 고장 조건에서 비교 실험이 수행된다.

Dynamic stability of FG-CNT-reinforced viscoelastic micro cylindrical shells resting on nonhomogeneous orthotropic viscoelastic medium subjected to harmonic temperature distribution and 2D magnetic field

  • Tohidi, H.;Hosseini-Hashemi, S.H.;Maghsoudpour, A.;Etemadi, S.
    • Wind and Structures
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    • 제25권2호
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    • pp.131-156
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    • 2017
  • This paper deals with the dynamic stability of embedded functionally graded (FG)-carbon nanotubes (CNTs)-reinforced micro cylindrical shells. The structure is subjected to harmonic non-uniform temperature distribution and 2D magnetic field. The CNT reinforcement is either uniformly distributed or FG along the thickness direction where the effective properties of nano-composite structure are estimated through Mixture low. The viscoelastic properties of structure are captured based on the Kelvin-Voigt theory. The surrounding viscoelastic medium is considered nonhomogeneous with the spring, orthotropic shear and damper constants. The material properties of cylindrical shell and the viscoelastic medium constants are assumed temperature-dependent. The first order shear deformation theory (FSDT) or Mindlin theory in conjunction with Hamilton's principle is utilized for deriving the motion equations where the size effects are considered based on Eringen's nonlocal theory. Based on differential quadrature (DQ) and Bolotin methods, the dynamic instability region (DIR) of structure is obtained for different boundary conditions. The effects of different parameters such as volume percent and distribution type of CNTs, mode number, viscoelastic medium type, temperature, boundary conditions, magnetic field, nonlocal parameter and structural damping constant are shown on the DIR of system. Numerical results indicate that the FGX distribution of CNTs is better than other considered cases. In addition, considering structural damping of system reduces the resonance frequency.

Nonlinear forced vibration of FG-CNTs-reinforced curved microbeam based on strain gradient theory considering out-of-plane motion

  • Allahkarami, Farshid;Nikkhah-bahrami, Mansour;Saryazdi, Maryam Ghassabzadeh
    • Steel and Composite Structures
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    • 제26권6호
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    • pp.673-691
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    • 2018
  • The main goal of this research is to examine the in-plane and out-of-plane forced vibration of a curved nanocomposite microbeam. The in-plane and out-of-plane displacements of the structure are considered based on the first order shear deformation theory (FSDT). The curved microbeam is reinforced by functionally graded carbon nanotubes (FG-CNTs) and thus the extended rule of mixture is employed to estimate the effective material properties of the structure. Also, the small scale effect is captured using the strain gradient theory. The structure is rested on a nonlinear orthotropic viscoelastic foundation and is subjected to concentrated transverse harmonic external force, thermal and magnetic loads. The derivation of the governing equations is performed using energy method and Hamilton's principle. Differential quadrature (DQ) method along with integral quadrature (IQ) and Newmark methods are employed to solve the problem. The effect of various parameters such as volume fraction and distribution type of CNTs, boundary conditions, elastic foundation, temperature changes, material length scale parameters, magnetic field, central angle and width to thickness ratio are studied on the frequency and force responses of the structure. The results indicate that the highest frequency and lowest vibration amplitude belongs to FGX distribution type while the inverse condition is observed for FGO distribution type. In addition, the hardening-type response of the structure with FGX distribution type is more intense with respect to the other distribution types.

A Simplified Synchronous Reference Frame for Indirect Current Controlled Three-level Inverter-based Shunt Active Power Filters

  • Hoon, Yap;Radzi, Mohd Amran Mohd;Hassan, Mohd Khair;Mailah, Nashiren Farzilah;Wahab, Noor Izzri Abdul
    • Journal of Power Electronics
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    • 제16권5호
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    • pp.1964-1980
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    • 2016
  • This paper presents a new simplified harmonics extraction algorithm based on the synchronous reference frame (SRF) for an indirect current controlled (ICC) three-level neutral point diode clamped (NPC) inverter-based shunt active power filter (SAPF). The shunt APF is widely accepted as one of the most effective current harmonics mitigation tools due to its superior adaptability in dynamic state conditions. In its controller, the SRF algorithm which is derived based on the direct-quadrature (DQ) theory has played a significant role as a harmonics extraction algorithm due to its simple implementation features. However, it suffers from significant delays due to its dependency on a numerical filter and unnecessary computation workloads. Moreover, the algorithm is mostly implemented for the direct current controlled (DCC) based SAPF which operates based on a non-sinusoidal reference current. This degrades the mitigation performances since the DCC based operation does not possess exact information on the actual source current which suffers from switching ripples problems. Therefore, three major improvements are introduced which include the development of a mathematical based fundamental component identifier to replace the numerical filter, the removal of redundant features, and the generation of a sinusoidal reference current. The proposed algorithm is developed and evaluated in MATLAB / Simulink. A laboratory prototype utilizing a TMS320F28335 digital signal processor (DSP) is also implemented to validate effectiveness of the proposed algorithm. Both simulation and experimental results are presented. They show significant improvements in terms of total harmonic distortion (THD) and dynamic response when compared to a conventional SRF algorithm.